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WO2009131043A1 - Micropuce - Google Patents

Micropuce Download PDF

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Publication number
WO2009131043A1
WO2009131043A1 PCT/JP2009/057573 JP2009057573W WO2009131043A1 WO 2009131043 A1 WO2009131043 A1 WO 2009131043A1 JP 2009057573 W JP2009057573 W JP 2009057573W WO 2009131043 A1 WO2009131043 A1 WO 2009131043A1
Authority
WO
WIPO (PCT)
Prior art keywords
liquid
microchip
dummy
reagent
reagent liquid
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/JP2009/057573
Other languages
English (en)
Japanese (ja)
Inventor
義一 栗原
継吾 玉木
彰久 中島
理英 村瀬
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Konica Minolta Medical and Graphic Inc
Original Assignee
Konica Minolta Medical and Graphic Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Konica Minolta Medical and Graphic Inc filed Critical Konica Minolta Medical and Graphic Inc
Priority to JP2010509151A priority Critical patent/JPWO2009131043A1/ja
Publication of WO2009131043A1 publication Critical patent/WO2009131043A1/fr
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

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Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/50273—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by the means or forces applied to move the fluids
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/10—Integrating sample preparation and analysis in single entity, e.g. lab-on-a-chip concept
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/14—Process control and prevention of errors
    • B01L2200/142—Preventing evaporation
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/16—Reagents, handling or storing thereof
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/02—Identification, exchange or storage of information
    • B01L2300/025—Displaying results or values with integrated means
    • B01L2300/027—Digital display, e.g. LCD, LED
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/06—Auxiliary integrated devices, integrated components
    • B01L2300/0627—Sensor or part of a sensor is integrated
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0809—Geometry, shape and general structure rectangular shaped
    • B01L2300/0816—Cards, e.g. flat sample carriers usually with flow in two horizontal directions
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0887—Laminated structure
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/10—Means to control humidity and/or other gases
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/18—Means for temperature control
    • B01L2300/1805—Conductive heating, heat from thermostatted solids is conducted to receptacles, e.g. heating plates, blocks
    • B01L2300/1822—Conductive heating, heat from thermostatted solids is conducted to receptacles, e.g. heating plates, blocks using Peltier elements
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/04—Moving fluids with specific forces or mechanical means
    • B01L2400/0475—Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure
    • B01L2400/0487—Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure fluid pressure, pneumatics
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/52—Containers specially adapted for storing or dispensing a reagent
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B65—CONVEYING; PACKING; STORING; HANDLING THIN OR FILAMENTARY MATERIAL
    • B65D—CONTAINERS FOR STORAGE OR TRANSPORT OF ARTICLES OR MATERIALS, e.g. BAGS, BARRELS, BOTTLES, BOXES, CANS, CARTONS, CRATES, DRUMS, JARS, TANKS, HOPPERS, FORWARDING CONTAINERS; ACCESSORIES, CLOSURES, OR FITTINGS THEREFOR; PACKAGING ELEMENTS; PACKAGES
    • B65D81/00—Containers, packaging elements, or packages, for contents presenting particular transport or storage problems, or adapted to be used for non-packaging purposes after removal of contents
    • B65D81/18—Containers, packaging elements, or packages, for contents presenting particular transport or storage problems, or adapted to be used for non-packaging purposes after removal of contents providing specific environment for contents, e.g. temperature above or below ambient
    • B65D81/20—Containers, packaging elements, or packages, for contents presenting particular transport or storage problems, or adapted to be used for non-packaging purposes after removal of contents providing specific environment for contents, e.g. temperature above or below ambient under vacuum or superatmospheric pressure, or in a special atmosphere, e.g. of inert gas
    • B65D81/2069—Containers, packaging elements, or packages, for contents presenting particular transport or storage problems, or adapted to be used for non-packaging purposes after removal of contents providing specific environment for contents, e.g. temperature above or below ambient under vacuum or superatmospheric pressure, or in a special atmosphere, e.g. of inert gas in a special atmosphere
    • B65D81/2084—Containers, packaging elements, or packages, for contents presenting particular transport or storage problems, or adapted to be used for non-packaging purposes after removal of contents providing specific environment for contents, e.g. temperature above or below ambient under vacuum or superatmospheric pressure, or in a special atmosphere, e.g. of inert gas in a special atmosphere in a flexible container
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
    • G01N35/00029—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor provided with flat sample substrates, e.g. slides
    • G01N2035/00099—Characterised by type of test elements
    • G01N2035/00148—Test cards, e.g. Biomerieux or McDonnel multiwell test cards

Definitions

  • the present invention relates to a microchip having a reagent liquid stored therein.
  • micromachine technology In recent years, by making full use of micromachine technology and ultrafine processing technology, devices and means (for example, pumps, valves, flow paths, sensors, etc.) for performing conventional sample preparation, chemical analysis, chemical synthesis, etc. have been miniaturized.
  • a system integrated on a chip has been developed (for example, Patent Document 1). This is also called ⁇ -TAS (Micro Total Analysis System).
  • a sample for example, urine, saliva, blood extracted from DNA subjected to DNA treatment
  • a reagent liquid In this method, the characteristics of the specimen are examined by mixing and detecting the reaction.
  • Microchip performs a photolithographic process (a method of creating a groove by etching a pattern image with a chemical) or a groove processing using laser light on a substrate made of a resin material or a glass material, and causes a reagent liquid or specimen to flow.
  • a fine flow path that can be used and a reservoir for storing reagent liquid are provided, and various patterns have been proposed (for example, Patent Document 1).
  • a small amount of reagent liquid in a fine channel and a sample are mixed at a predetermined mixing ratio to perform a reaction or the like.
  • it is necessary to accurately control the mixing ratio of the two, and for that purpose, it is important to manage the amount of the reagent liquid.
  • a reagent liquid in advance in the microchip and newly inject only the sample when performing the test.
  • the reagent liquid will evaporate, so the mixing ratio can be controlled. May be difficult.
  • Patent Document 2 discloses a microchip that prevents the reagent liquid from evaporating by sealing the outside of the reagent liquid in the flow path with an inert liquid.
  • an object of the present invention is to provide a microchip having a reagent liquid stored therein that can be stored for a long period of time.
  • a microchip stored in a packaging bag having a moisture barrier property or a gas barrier property A microfluidic channel; a reagent liquid reservoir that stores a reagent liquid for feeding the microfluidic channel and mixing it with a sample; a dummy liquid reservoir that stores a dummy liquid for evaporation; and the dummy liquid An opening connected to the reservoir,
  • microchip as described in 1 above, further comprising an inlet for injecting the reagent liquid into the reagent liquid reservoir.
  • microchip housed in the packaging bag, wherein the injection port is closed by a sealing member, and the opening is not closed. Microchip.
  • the present invention it is possible to provide a microchip that can be stored for a long period of time and stores a reagent liquid therein.
  • FIG. 2A is a top view of the microchip 100
  • FIG. 2B is a side view of the microchip 100
  • FIG. 1 is a schematic diagram showing an internal structure of a microchip 100.
  • FIG. It is a perspective view of the microchip 100 accommodated in the packaging bag 5 in this embodiment.
  • FIG. 5A is a top view of the microchip 100 according to the second embodiment
  • FIG. 5B is a schematic diagram showing the internal structure of the microchip 100. It is a graph which shows transition of the evaporation amount of the reagent liquid with the elapsed days in an Example and a comparative example.
  • microchip refers to a chip in a micro total analysis system used for various applications such as synthesis and inspection, and “microchip” particularly used for inspection of biological materials.
  • the “fine channel” may refer only to a narrow channel portion excluding a structure portion that may be formed wide, but in a broad sense includes such a structure portion.
  • the fluid flowing in the communicating fine flow path is actually a liquid, and specifically, various reagent liquids, sample liquids, denaturant liquids, cleaning liquids, driving liquids, and the like are applicable.
  • the present invention can be applied to a reaction detection apparatus using a microchip regardless of the use of the microchip.
  • embodiments of the present invention will be described with reference to the drawings.
  • FIG. 1 is a schematic diagram illustrating an example of a micro analysis system.
  • FIG. 1 shows a state in which the microchip 100 according to the present embodiment is loaded on the inspection apparatus 20 that is the micro-analysis system according to the present embodiment.
  • the inspection apparatus 20 is included in a micropump unit 210 that feeds liquid in the microchip, a heating / cooling unit 230 for promoting and suppressing the reaction in the microchip, and a product liquid obtained by the reaction in the microchip.
  • a detection unit 250 that detects the target substance to be detected
  • a drive control unit 270 that drives, controls, and detects each unit in the inspection apparatus.
  • the micropump unit 210 includes a micropump 211 that performs liquid feeding, a chip connection unit 213 that connects the micropump 211 and the microchip 100, a driving liquid tank 215 that is filled with driving liquid for liquid feeding, and a driving liquid tank 215.
  • the driving liquid supply unit 217 and the like for supplying the driving liquid to the micro pump 211.
  • the driving liquid tank 215 can be removed and replaced from the driving liquid supply unit 217 for replenishment of the driving liquid.
  • the micropump 211 is formed by one or a plurality of pumps. In the case of a plurality of micropumps 211, the micropumps 211 can be driven independently or in conjunction with each other.
  • the heating / cooling unit 230 includes a cooling unit 231 configured with a Peltier element and the like, a heating unit 233 configured with a heater and the like. Of course, the heating unit may also be composed of Peltier elements.
  • the detection unit 250 includes a light emitting diode (LED) 251 and a light receiving element (PD) 253, and optically detects a target substance contained in a product solution obtained by a reaction in the microchip.
  • LED light emitting diode
  • PD light receiving element
  • the microchip 100 and the micropump 211 are connected and communicated with each other via a chip connection unit 213, and when the micropump 211 is driven, various reagent liquids and specimens stored in a plurality of storage units in the microchip 100 are transferred. Then, the liquid is fed through the air by the driving liquid flowing into the microchip 100 from the micropump 211 via the chip connection portion 213.
  • FIG. 2A is a top view of the microchip 100
  • FIG. 2B is a side view of the microchip 100.
  • the microchip 100 includes a groove forming substrate 108 and a covering substrate 109 that covers the groove forming substrate 108.
  • the groove forming substrate 108 is formed with respective structural parts such as a reagent liquid storage part, which will be described later, and fine flow channels r1 and r2 (see FIG. 3) for communicating these structural parts.
  • the width and height of the fine channels r1 and r2 are about several ⁇ m to several hundred ⁇ m.
  • the size of the microchip 100 is usually about several tens mm in length and width and about several mm in height.
  • the microchip 100 is configured by bringing the covering substrate 109 into close contact with the groove forming substrate 108 and covering these structural portions and the fine flow path.
  • at least to-be-detected part needs to use the light-transmitting material with respect to detection light among said structure parts.
  • the microchip 100 is usually manufactured by appropriately combining one or more molding materials.
  • the molding material for the microchip 100 include plastic resins, various inorganic glasses, silicon, ceramics, and metals.
  • plastic resins plastic resins
  • the substrate for forming and processing the flow path such as the groove forming substrate 108, the flow path is not easily deformed due to water absorption, and a hydrophobic and water-repellent plastic is used so that a small amount of sample liquid can be fed without loss in the middle. preferable.
  • polystyrene examples include resins such as polystyrene, polyethylene, polypropylene, polyethylene terephthalate, polyethylene naphthalate, polyethylene vinyl alcohol, polycarbonate, polymethylpentene, fluorocarbon, and saturated cyclic polyolefin.
  • resins such as polystyrene, polyethylene, polypropylene, polyethylene terephthalate, polyethylene naphthalate, polyethylene vinyl alcohol, polycarbonate, polymethylpentene, fluorocarbon, and saturated cyclic polyolefin.
  • polystyrene is preferable as a material for forming the groove forming substrate 108 because it is excellent in transparency, mechanical properties, and moldability, and can be finely processed.
  • the coated substrate 109 is made of a flexible material such as polyolefin, and is provided with openings (holes) that connect the internal structures and the outside air, or the fine flow path and the outside air.
  • openings include an injection port for injecting a reagent liquid or a specimen sample, a connection port connected to a micropump via a chip connection portion, and various holes opened to the outside air.
  • polyolefins include polypropylene, polycarbonate, polystyrene, polyethylene, and cycloolefin polymers.
  • FIG. 3 is a schematic diagram showing the internal structure of the microchip 100 and shows a state where the covering substrate 109 is removed.
  • 133a and 133b are reagent liquid storage units for storing reagent liquids
  • 137 is a sample storage unit for storing sample samples.
  • constituent elements are generically indicated by reference numerals with alphabetic suffixes omitted, and individual constituent elements are indicated by reference numerals with suffixes.
  • an inlet i1 for injecting a reagent liquid and an inlet i3 for injecting a specimen sample are provided beside each storage unit.
  • connection ports 132a and 132b opened from one surface of the microchip 100 to the outside are provided on the upstream side in the liquid feeding direction of the storage unit.
  • the microchip 100 is connected to the micropump 211 through the connection ports 132 a and 132 b and the chip connection part 213 so as to be connected to the micropump 211.
  • a reaction unit 139 that mixes and reacts the reagent liquid from the reagent liquid storage unit 133 and the liquid from the sample storage unit 137 is provided downstream of the reagent liquid storage unit 133 and the sample storage unit 137.
  • a detected part 148 is provided downstream of the reaction part 139, and a waste liquid part 60 is provided further downstream.
  • the detection unit 250 detects the reaction of the detected unit 148.
  • the reagent liquid stored in the reagent liquid storage part 133 is driven via air by the driving liquid sent from the micro pump 211 communicating with the connection port 132a (flowed) and flows into the reaction part 139.
  • the sample stored in the specimen storage unit 137 is driven by the driving liquid sent from the separate micro pump 211 communicating with the connection port 132b and flows into the reaction unit 139.
  • the reaction part 139 the reagent liquid sent from the reagent liquid storage part 133 and the sample sent from the sample storage part 137 are mixed.
  • the reagent liquid and the sample mixed in the reaction unit 139 are heated by the heating unit 233 provided in the inspection apparatus 20 to start the reaction.
  • the liquid after the reaction is sent to the detected part 148.
  • the target substance is detected by, for example, an optical detection method.
  • the liquid detected by the detected part 148 is sent to the waste liquid part 60.
  • the present invention is not limited to this, and the pressurized air is directly connected by the syringe pump.
  • the reagent liquid or the like may be fed by being fed into the port 132.
  • the dummy liquid storage unit 150 stores a dummy liquid.
  • the dummy liquid is not used for inspection. If the reagent liquid is an aqueous solution, the dummy liquid uses an aqueous solution or pure water. If the reagent liquid is an alcohol solution, the dummy liquid also uses an alcohol solution or alcohol. By doing so, the dummy liquid or its solvent is shared with the reagent liquid solvent. That is, the evaporation component from the dummy liquid is common with at least a part of the evaporation component from the reagent liquid, and therefore the evaporation component from the dummy liquid (or its solvent) that more actively evaporates causes the inside of the packaging bag 5 to be inside. Can be prevented from evaporating from the reagent liquid using the same solvent.
  • dummy liquids not only one type of dummy liquid but also different types of dummy liquids may be stored in the plurality of dummy liquid storage units 150.
  • reagent liquids using different types of solvents are stored in the reagent liquid storage units 133a and 133b, it is preferable to use dummy liquids corresponding to the plurality of types of solvents.
  • the dummy liquid is injected from the opening i2 into the dummy liquid storage unit 150, and the opening i2 and the dummy liquid storage unit 150 are connected by the fine flow path r1.
  • the dummy liquid storage unit 150 is connected to a fine channel r2 having a different path from the fine channel r1 to which the reagent liquid storage unit 133 storing the reagent liquid is connected.
  • FIG. 4 is a perspective view of the microchip 100 housed in the packaging bag according to the present embodiment.
  • Reference numeral 5 denotes a packaging bag in which the microchip 100 is accommodated in an inside sealed with an adhesive 51.
  • the packaging bag 5 is made of a flexible packaging material having gas barrier properties or moisture barrier properties.
  • the “packaging bag having moisture barrier property or gas barrier property” of the present invention refers to a packaging bag formed of a packaging material having a performance higher than a moisture permeability of 10.0 g / m 2 / day.
  • the moisture permeability is preferably 0.5 g / m2 / day or less, and more preferably 0.05 g / m2 / day or less.
  • the moisture permeability is measured under the conditions of 40 ° C. and 90% RH according to the method described in JIS-Z-0208. The lower the numerical value of moisture permeability, the better because the gas in which the liquid inside has evaporated does not leak to the outside of the packaging bag.
  • the microchip 100 in which the dummy liquid of the same system as the reagent liquid is stored is accommodated in the packaging bag 5 having a low value of moisture permeability of 0.05 g / m 2 / day.
  • the dummy liquid also evaporates along with the reagent liquid, so that the inside of the packaging bag 5 can be quickly brought into a saturated state of the evaporated gas (saturated water vapor state if water). Thereby, evaporation derived from the reagent liquid can be suppressed.
  • the dummy liquid can be stored in a larger amount than the reagent liquid, and the dummy liquid storage portion Since the flow path 150 communicates with the plurality of openings i2, the evaporation of the dummy liquid is more dominant than the evaporation of the reagent liquid, so that the evaporation of the reagent liquid can be further suppressed.
  • the dummy liquid storage part 150 and the opening part i2 of the dummy liquid are connected by the fine flow path r2, excessive evaporation can be prevented and, in turn, the occurrence of condensation within the packaging bag 5 can be suppressed. it can. Further, since the liquid is connected to the outside via the fine channel r2, the liquid dummy liquid is difficult to move in the fine channel r2 due to its surface tension. As a result, even when the microchip 100 is tilted during transportation or the like, the liquid dummy liquid can be prevented from leaking outside the microchip 100.
  • the injection port i1 of the microchip 100 Prior to being housed in the packaging bag 5, the injection port i1 of the microchip 100 is closed with a sealing member such as a thermoplastic resin, and the opening i2 is not closed. .
  • the microchip 100 in such a state is stored in the packaging bag 5.
  • the reagent liquid in the reagent liquid storage unit 133 does not come into direct contact with the outside air. Therefore, the evaporation of the reagent liquid to the outside of the microchip 100 (inside the packaging bag 5) occurs on the groove forming substrate 108. Only the amount that evaporates through the substrate.
  • the packaging bag 5 is made of a material having a moisture barrier property or a gas barrier property, the reagent liquid is difficult to evaporate after becoming saturated, and thus the evaporation of the reagent liquid can be further suppressed. Thereby, the amount of the reagent liquid can be accurately maintained over a long period of time, and a long-term storage of the microchip in which the reagent liquid is stored can be realized.
  • FIG. 5A is a top view of the microchip 100 according to the second embodiment
  • FIG. 5B is a schematic diagram showing the internal structure of the microchip 100. 2 correspond to FIG. 2A and FIG. 3, respectively, and components having the same functions as those of the microchip 100 in the embodiment shown in FIG. 2, FIG.
  • the microchip 100 in the second embodiment is not provided with the injection ports i1 and i3. Instead of the opening i2 for evaporating the dummy liquid, a line of openings g is newly provided.
  • the dummy liquid is injected into the dummy liquid reservoir 150 and the reagent liquid is injected into the reagent liquid reservoir 133.
  • the injection port can be omitted and there is no need to seal with a sealing member.
  • injection of the specimen sample into the specimen reservoir 137 is performed, for example, by inserting a syringe needle into the coated substrate 109 above the specimen reservoir 137.
  • the opening g is provided above the fine channel r2 adjacent to the dummy liquid reservoir 150, and the opening g and the dummy liquid reservoir 150 are communicated with each other via the fine channel r1.
  • the dummy liquid is evaporated from the opening g.
  • FIG. 6 is a graph showing the transition of the evaporation amount of the reagent liquid with the elapsed days in the example and the comparative example.
  • evaporation occurs initially but tends to be saturated.
  • the reduction in reagent liquid due to evaporation at saturation is approximately 1%.
  • the comparative example it can be seen that the evaporation rate of the reagent liquid is high, and no saturation tendency is observed, and evaporation continues at a constant rate.
  • the decrease in reagent liquid due to evaporation is about 6% when 120 days have passed.
  • the storage period is longer than 120 days, since the saturation tendency is not shown in the embodiment, the difference further increases.

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  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Dispersion Chemistry (AREA)
  • Analytical Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Hematology (AREA)
  • Clinical Laboratory Science (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Automatic Analysis And Handling Materials Therefor (AREA)

Abstract

L'invention porte sur une micropuce qui peut stocker en interne un réactif liquide pendant une longue période de temps. La micropuce comporte par conséquent un microcanal (r1), un réservoir de réactif liquide (133) qui stocke un réactif liquide qui est transféré à travers le microcanal (r1) et mélangé à un échantillon, et un réservoir de liquide factice (150) qui stocke un liquide factice pour une évaporation. La micropuce est stockée dans un sac de conditionnement ayant des propriétés de barrière contre l'humidité ou des propriétés de barrière contre les gaz.
PCT/JP2009/057573 2008-04-25 2009-04-15 Micropuce Ceased WO2009131043A1 (fr)

Priority Applications (1)

Application Number Priority Date Filing Date Title
JP2010509151A JPWO2009131043A1 (ja) 2008-04-25 2009-04-15 マイクロチップ

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
JP2008115105 2008-04-25
JP2008-115105 2008-04-25

Publications (1)

Publication Number Publication Date
WO2009131043A1 true WO2009131043A1 (fr) 2009-10-29

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PCT/JP2009/057573 Ceased WO2009131043A1 (fr) 2008-04-25 2009-04-15 Micropuce

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JP (1) JPWO2009131043A1 (fr)
WO (1) WO2009131043A1 (fr)

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2013160648A (ja) * 2012-02-06 2013-08-19 Sony Corp マイクロチップ
JP2015142893A (ja) * 2014-01-31 2015-08-06 株式会社日立製作所 医薬品提供システムおよび医薬品提供方法
WO2019097984A1 (fr) * 2017-11-15 2019-05-23 コニカミノルタ株式会社 Boîtier d'inspection

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2006234590A (ja) * 2005-02-25 2006-09-07 Shimadzu Corp 反応容器
JP2006521829A (ja) * 2003-04-03 2006-09-28 フルイディグム コーポレイション マイクロ流体装置およびその使用方法

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2006521829A (ja) * 2003-04-03 2006-09-28 フルイディグム コーポレイション マイクロ流体装置およびその使用方法
JP2006234590A (ja) * 2005-02-25 2006-09-07 Shimadzu Corp 反応容器

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JP2013160648A (ja) * 2012-02-06 2013-08-19 Sony Corp マイクロチップ
JP2015142893A (ja) * 2014-01-31 2015-08-06 株式会社日立製作所 医薬品提供システムおよび医薬品提供方法
US10005060B2 (en) 2014-01-31 2018-06-26 Hitachi, Ltd. Drug provision system and drug provision method
WO2019097984A1 (fr) * 2017-11-15 2019-05-23 コニカミノルタ株式会社 Boîtier d'inspection
JPWO2019097984A1 (ja) * 2017-11-15 2020-11-19 コニカミノルタ株式会社 検査パッケージ
JP7237008B2 (ja) 2017-11-15 2023-03-10 大塚製薬株式会社 検査パッケージ

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