[go: up one dir, main page]

WO2009032779A3 - Methods and compositions for the size-specific seperation of nucleic acid from a sample - Google Patents

Methods and compositions for the size-specific seperation of nucleic acid from a sample Download PDF

Info

Publication number
WO2009032779A3
WO2009032779A3 PCT/US2008/074689 US2008074689W WO2009032779A3 WO 2009032779 A3 WO2009032779 A3 WO 2009032779A3 US 2008074689 W US2008074689 W US 2008074689W WO 2009032779 A3 WO2009032779 A3 WO 2009032779A3
Authority
WO
WIPO (PCT)
Prior art keywords
nucleic acid
methods
seperation
compositions
sample
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/US2008/074689
Other languages
French (fr)
Other versions
WO2009032779A2 (en
Inventor
Min Seob Lee
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Sequenom Inc
Original Assignee
Sequenom Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Sequenom Inc filed Critical Sequenom Inc
Publication of WO2009032779A2 publication Critical patent/WO2009032779A2/en
Publication of WO2009032779A3 publication Critical patent/WO2009032779A3/en
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6858—Allele-specific amplification

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Analytical Chemistry (AREA)
  • Biophysics (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Physics & Mathematics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

Provided herein are methods and kits for the separation, extraction and enrichment of short- stranded nucleic acid in the presence of a high background of long-stranded genomic material (e.g., host or maternal nucleic acids). The methods rely on primers that selectively bind to the long- stranded nucleic acid, whereby the primers may be used to subsequently separate the long and short nucleic acid.
PCT/US2008/074689 2007-08-29 2008-08-28 Methods and compositions for the size-specific seperation of nucleic acid from a sample Ceased WO2009032779A2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US96887607P 2007-08-29 2007-08-29
US60/968,876 2007-08-29

Publications (2)

Publication Number Publication Date
WO2009032779A2 WO2009032779A2 (en) 2009-03-12
WO2009032779A3 true WO2009032779A3 (en) 2009-05-07

Family

ID=40429663

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2008/074689 Ceased WO2009032779A2 (en) 2007-08-29 2008-08-28 Methods and compositions for the size-specific seperation of nucleic acid from a sample

Country Status (1)

Country Link
WO (1) WO2009032779A2 (en)

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9367663B2 (en) 2011-10-06 2016-06-14 Sequenom, Inc. Methods and processes for non-invasive assessment of genetic variations
US9404150B2 (en) 2007-08-29 2016-08-02 Sequenom, Inc. Methods and compositions for universal size-specific PCR
US9580741B2 (en) 2009-04-03 2017-02-28 Sequenom, Inc. Nucleic acid preparation compositions and methods

Families Citing this family (101)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US10083273B2 (en) 2005-07-29 2018-09-25 Natera, Inc. System and method for cleaning noisy genetic data and determining chromosome copy number
US11111543B2 (en) 2005-07-29 2021-09-07 Natera, Inc. System and method for cleaning noisy genetic data and determining chromosome copy number
US10081839B2 (en) 2005-07-29 2018-09-25 Natera, Inc System and method for cleaning noisy genetic data and determining chromosome copy number
US11111544B2 (en) 2005-07-29 2021-09-07 Natera, Inc. System and method for cleaning noisy genetic data and determining chromosome copy number
US9424392B2 (en) 2005-11-26 2016-08-23 Natera, Inc. System and method for cleaning noisy genetic data from target individuals using genetic data from genetically related individuals
EP3260556B1 (en) 2006-05-31 2019-07-31 Sequenom, Inc. Methods for the extraction of nucleic acid from a sample
US7902345B2 (en) 2006-12-05 2011-03-08 Sequenom, Inc. Detection and quantification of biomolecules using mass spectrometry
CA2680588A1 (en) 2007-03-26 2008-10-02 Sequenom, Inc. Restriction endonuclease enhanced polymorphic sequence detection
US7888127B2 (en) 2008-01-15 2011-02-15 Sequenom, Inc. Methods for reducing adduct formation for mass spectrometry analysis
EP2276858A4 (en) 2008-03-26 2011-10-05 Sequenom Inc RESULTS IMPROVED BY POLYMORPHIC SEQUENCES IMPROVED BY RESTRICTION ENDUCLEASE
CA3116156C (en) 2008-08-04 2023-08-08 Natera, Inc. Methods for allele calling and ploidy calling
US8476013B2 (en) 2008-09-16 2013-07-02 Sequenom, Inc. Processes and compositions for methylation-based acid enrichment of fetal nucleic acid from a maternal sample useful for non-invasive prenatal diagnoses
US8962247B2 (en) 2008-09-16 2015-02-24 Sequenom, Inc. Processes and compositions for methylation-based enrichment of fetal nucleic acid from a maternal sample useful for non invasive prenatal diagnoses
CN102597266A (en) 2009-09-30 2012-07-18 纳特拉公司 Methods for non-invasive prenatal ploidy calling
EP3088532B1 (en) 2009-12-22 2019-10-30 Sequenom, Inc. Processes and kits for identifying aneuploidy
SG185544A1 (en) * 2010-05-14 2012-12-28 Fluidigm Corp Nucleic acid isolation methods
US9677118B2 (en) 2014-04-21 2017-06-13 Natera, Inc. Methods for simultaneous amplification of target loci
US12545960B2 (en) 2010-05-18 2026-02-10 Natera, Inc. Methods for simultaneous amplification of target loci
US11326208B2 (en) 2010-05-18 2022-05-10 Natera, Inc. Methods for nested PCR amplification of cell-free DNA
US11332793B2 (en) 2010-05-18 2022-05-17 Natera, Inc. Methods for simultaneous amplification of target loci
US10316362B2 (en) 2010-05-18 2019-06-11 Natera, Inc. Methods for simultaneous amplification of target loci
US11339429B2 (en) 2010-05-18 2022-05-24 Natera, Inc. Methods for non-invasive prenatal ploidy calling
US20190010543A1 (en) 2010-05-18 2019-01-10 Natera, Inc. Methods for simultaneous amplification of target loci
CA3207599A1 (en) 2010-05-18 2011-11-24 Natera, Inc. Methods for non-invasive prenatal ploidy calling
US11332785B2 (en) 2010-05-18 2022-05-17 Natera, Inc. Methods for non-invasive prenatal ploidy calling
US11322224B2 (en) 2010-05-18 2022-05-03 Natera, Inc. Methods for non-invasive prenatal ploidy calling
US11939634B2 (en) 2010-05-18 2024-03-26 Natera, Inc. Methods for simultaneous amplification of target loci
US12152275B2 (en) 2010-05-18 2024-11-26 Natera, Inc. Methods for non-invasive prenatal ploidy calling
US12221653B2 (en) 2010-05-18 2025-02-11 Natera, Inc. Methods for simultaneous amplification of target loci
US11408031B2 (en) 2010-05-18 2022-08-09 Natera, Inc. Methods for non-invasive prenatal paternity testing
RU2620959C2 (en) 2010-12-22 2017-05-30 Натера, Инк. Methods of noninvasive prenatal paternity determination
JP6153874B2 (en) 2011-02-09 2017-06-28 ナテラ， インコーポレイテッド Method for non-invasive prenatal ploidy calls
US20140235474A1 (en) 2011-06-24 2014-08-21 Sequenom, Inc. Methods and processes for non invasive assessment of a genetic variation
US10196681B2 (en) 2011-10-06 2019-02-05 Sequenom, Inc. Methods and processes for non-invasive assessment of genetic variations
US10424394B2 (en) 2011-10-06 2019-09-24 Sequenom, Inc. Methods and processes for non-invasive assessment of genetic variations
WO2013052907A2 (en) 2011-10-06 2013-04-11 Sequenom, Inc. Methods and processes for non-invasive assessment of genetic variations
US9984198B2 (en) 2011-10-06 2018-05-29 Sequenom, Inc. Reducing sequence read count error in assessment of complex genetic variations
ES2886508T3 (en) 2011-10-06 2021-12-20 Sequenom Inc Methods and procedures for the non-invasive evaluation of genetic variations
US8688388B2 (en) 2011-10-11 2014-04-01 Sequenom, Inc. Methods and processes for non-invasive assessment of genetic variations
EP3243908B1 (en) 2011-10-11 2019-01-02 Sequenom, Inc. Methods and processes for non-invasive assessment of genetic variations
CA2861856C (en) 2012-01-20 2020-06-02 Sequenom, Inc. Diagnostic processes that factor experimental conditions
US9605313B2 (en) 2012-03-02 2017-03-28 Sequenom, Inc. Methods and processes for non-invasive assessment of genetic variations
US9920361B2 (en) 2012-05-21 2018-03-20 Sequenom, Inc. Methods and compositions for analyzing nucleic acid
ES2902401T3 (en) 2012-05-21 2022-03-28 Sequenom Inc Methods and processes for the non-invasive evaluation of genetic variations
US10504613B2 (en) 2012-12-20 2019-12-10 Sequenom, Inc. Methods and processes for non-invasive assessment of genetic variations
US10497461B2 (en) 2012-06-22 2019-12-03 Sequenom, Inc. Methods and processes for non-invasive assessment of genetic variations
HK1206792A1 (en) 2012-07-13 2016-01-15 Sequenom, Inc. Processes and compositions for methylation-based enrichment of fetal nucleic acid from a maternal sample useful for non-invasive prenatal diagnoses
AU2012385961B9 (en) * 2012-07-24 2017-05-18 Natera, Inc. Highly multiplex PCR methods and compositions
US20140100126A1 (en) 2012-08-17 2014-04-10 Natera, Inc. Method for Non-Invasive Prenatal Testing Using Parental Mosaicism Data
US10482994B2 (en) 2012-10-04 2019-11-19 Sequenom, Inc. Methods and processes for non-invasive assessment of genetic variations
EP2904534B1 (en) 2012-10-04 2021-12-15 Sequenom, Inc. Methods and processes for non-invasive assessment of genetic variations
US20130309666A1 (en) 2013-01-25 2013-11-21 Sequenom, Inc. Methods and processes for non-invasive assessment of genetic variations
US20130189684A1 (en) 2013-03-12 2013-07-25 Sequenom, Inc. Quantification of cell-specific nucleic acid markers
EP3597774A1 (en) 2013-03-13 2020-01-22 Sequenom, Inc. Primers for dna methylation analysis
US9305756B2 (en) 2013-03-13 2016-04-05 Agena Bioscience, Inc. Preparation enhancements and methods of use for MALDI mass spectrometry
HUE061261T2 (en) 2013-04-03 2023-05-28 Sequenom Inc Methods and processes for non-invasive assessment of genetic variations
IL309903A (en) 2013-05-24 2024-03-01 Sequenom Inc Methods and processes for non-invasive evaluation of genetic variations
ES3037160T3 (en) 2013-06-21 2025-09-29 Sequenom Inc Methods and processes for non-invasive assessment of genetic variations
US10577655B2 (en) 2013-09-27 2020-03-03 Natera, Inc. Cell free DNA diagnostic testing standards
US9499870B2 (en) 2013-09-27 2016-11-22 Natera, Inc. Cell free DNA diagnostic testing standards
US10262755B2 (en) 2014-04-21 2019-04-16 Natera, Inc. Detecting cancer mutations and aneuploidy in chromosomal segments
DK3053071T3 (en) 2013-10-04 2024-01-22 Sequenom Inc METHODS AND PROCESSES FOR NON-INVASIVE ASSESSMENT OF GENETIC VARIATIONS
CA2925111C (en) 2013-10-07 2024-01-16 Sequenom, Inc. Methods and processes for non-invasive assessment of chromosome alterations
US11365447B2 (en) 2014-03-13 2022-06-21 Sequenom, Inc. Methods and processes for non-invasive assessment of genetic variations
WO2015159979A1 (en) * 2014-04-18 2015-10-22 凸版印刷株式会社 Method for recovering short-chain nucleic acids
US12492429B2 (en) 2014-04-21 2025-12-09 Natera, Inc. Detecting mutations and ploidy in chromosomal segments
EP3957749A1 (en) 2014-04-21 2022-02-23 Natera, Inc. Detecting tumour specific mutations in biopsies with whole exome sequencing and in cell-free samples
CA3191504A1 (en) 2014-05-30 2015-12-03 Sequenom, Inc. Chromosome representation determinations
US20180173846A1 (en) 2014-06-05 2018-06-21 Natera, Inc. Systems and Methods for Detection of Aneuploidy
ES3047391T3 (en) 2014-07-30 2025-12-03 Sequenom Inc Methods and processes for non-invasive assessment of genetic variations
EP3730629A1 (en) 2014-10-10 2020-10-28 Sequenom, Inc. Methods and processes for non-invasive assessment of genetic variations
EP4428863A3 (en) 2015-05-11 2024-12-11 Natera, Inc. Methods and compositions for determining ploidy
CN109477138A (en) 2016-04-15 2019-03-15 纳特拉公司 Lung cancer detection method
EP3464626B1 (en) 2016-05-27 2022-04-06 Sequenom, Inc. Methods for detecting genetic variations
US11299780B2 (en) 2016-07-15 2022-04-12 The Regents Of The University Of California Methods of producing nucleic acid libraries
WO2018022890A1 (en) 2016-07-27 2018-02-01 Sequenom, Inc. Genetic copy number alteration classifications
CA3030894A1 (en) 2016-07-27 2018-02-01 Sequenom, Inc. Methods for non-invasive assessment of genomic instability
US11485996B2 (en) 2016-10-04 2022-11-01 Natera, Inc. Methods for characterizing copy number variation using proximity-litigation sequencing
GB201618485D0 (en) 2016-11-02 2016-12-14 Ucl Business Plc Method of detecting tumour recurrence
US10011870B2 (en) 2016-12-07 2018-07-03 Natera, Inc. Compositions and methods for identifying nucleic acid molecules
CA3194557A1 (en) 2017-01-20 2018-07-26 Sequenom, Inc. Sequencing adapter manufacture and use
EP3571614A1 (en) 2017-01-20 2019-11-27 Sequenom, Inc. Methods for non-invasive assessment of copy number alterations
EP3571615B1 (en) 2017-01-20 2024-01-24 Sequenom, Inc. Methods for non-invasive assessment of genetic alterations
EP3574424A1 (en) 2017-01-24 2019-12-04 Sequenom, Inc. Methods and processes for assessment of genetic variations
CA3049139A1 (en) 2017-02-21 2018-08-30 Natera, Inc. Compositions, methods, and kits for isolating nucleic acids
US12421550B2 (en) 2017-03-17 2025-09-23 Sequenom, Inc. Methods and processes for assessment of genetic mosaicism
US12084720B2 (en) 2017-12-14 2024-09-10 Natera, Inc. Assessing graft suitability for transplantation
EP3737774B1 (en) 2018-01-12 2024-12-25 Claret Bioscience, LLC Method for analyzing nucleic acid
US12398389B2 (en) 2018-02-15 2025-08-26 Natera, Inc. Methods for isolating nucleic acids with size selection
WO2019200228A1 (en) 2018-04-14 2019-10-17 Natera, Inc. Methods for cancer detection and monitoring by means of personalized detection of circulating tumor dna
WO2019236726A1 (en) 2018-06-06 2019-12-12 The Regents Of The University Of California Methods of producing nucleic acid libraries and compositions and kits for practicing same
US12234509B2 (en) 2018-07-03 2025-02-25 Natera, Inc. Methods for detection of donor-derived cell-free DNA
EP4538390A3 (en) 2019-04-05 2025-07-09 Claret Bioscience, LLC Methods and compositions for analyzing nucleic acid
CN114144531A (en) * 2019-05-23 2022-03-04 美国分子实验室公司 Method for detecting rare DNA sequences in fecal samples
US12305235B2 (en) 2019-06-06 2025-05-20 Natera, Inc. Methods for detecting immune cell DNA and monitoring immune system
AU2020363787A1 (en) 2019-10-09 2022-04-21 Claret Bioscience, Llc Methods and compositions for analyzing nucleic acid
EP4052259A1 (en) 2019-10-31 2022-09-07 Sequenom, Inc. Application of mosaicism ratio in multifetal gestations and personalized risk assessment
JP7802707B2 (en) 2020-06-24 2026-01-20 クラレット バイオサイエンス， エルエルシー Methods and compositions for analyzing nucleic acids
WO2022076574A1 (en) 2020-10-08 2022-04-14 Claret Bioscience, Llc Methods and compositions for analyzing nucleic acid
WO2024186778A1 (en) 2023-03-03 2024-09-12 Laboratory Corporation Of America Holdings Methods and systems for positive cfdna screening on genetic variations using mosaicism ratio
AU2024271681A1 (en) 2023-05-15 2025-09-18 Laboratory Corporation Of America Holdings Machine-learning approaches to pan-cancer screening in whole genome sequencing

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5387505A (en) * 1990-05-04 1995-02-07 Eastman Kodak Company Preparation and isolation of single-stranded biotinylated nucleic acids by heat avidin-biotin cleavage
US5484701A (en) * 1990-01-26 1996-01-16 E. I. Du Pont De Nemours And Company Method for sequencing DNA using biotin-strepavidin conjugates to facilitate the purification of primer extension products
US20010031467A1 (en) * 1999-12-10 2001-10-18 Johannes Dapprich Method for selectively isolating a nucleic acid
US20020022224A1 (en) * 2000-04-28 2002-02-21 Hornby David P. Method for isolating single-stranded DNA

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5484701A (en) * 1990-01-26 1996-01-16 E. I. Du Pont De Nemours And Company Method for sequencing DNA using biotin-strepavidin conjugates to facilitate the purification of primer extension products
US5387505A (en) * 1990-05-04 1995-02-07 Eastman Kodak Company Preparation and isolation of single-stranded biotinylated nucleic acids by heat avidin-biotin cleavage
US20010031467A1 (en) * 1999-12-10 2001-10-18 Johannes Dapprich Method for selectively isolating a nucleic acid
US20020022224A1 (en) * 2000-04-28 2002-02-21 Hornby David P. Method for isolating single-stranded DNA

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9404150B2 (en) 2007-08-29 2016-08-02 Sequenom, Inc. Methods and compositions for universal size-specific PCR
US9580741B2 (en) 2009-04-03 2017-02-28 Sequenom, Inc. Nucleic acid preparation compositions and methods
US9367663B2 (en) 2011-10-06 2016-06-14 Sequenom, Inc. Methods and processes for non-invasive assessment of genetic variations

Also Published As

Publication number Publication date
WO2009032779A2 (en) 2009-03-12

Similar Documents

Publication Publication Date Title
WO2009032779A3 (en) Methods and compositions for the size-specific seperation of nucleic acid from a sample
WO2007140417A3 (en) Methods and compositions for the extraction and amplification of nucleic acid from a sample
WO2010115016A3 (en) Nucleic acid preparation compositions and methods
WO2009048530A3 (en) Highly multiplexed particle-based assays
WO2008092016A3 (en) Methods, compositions, and kits for detection of micro rna
WO2008002740A3 (en) Methods, compositions, and kits for rna extraction
WO2009049916A3 (en) Method for determining methylation at cytosine residues
WO2009032781A3 (en) Methods and compositions for universal size-specific polymerase chain reaction
WO2008105896A3 (en) Analyte determination utilizing mass tagging reagents comprising a non-encoded detectable label
WO2010028382A3 (en) Collecting and processing complex macromolecular mixtures
EP4442366A3 (en) Extreme pcr
WO2007092916A3 (en) Methods of extracting rna
EP4219746A3 (en) Analysis of chromatin using a nicking enzyme
WO2009015863A3 (en) Methods of detecting methylated dna at a specific locus
WO2010107816A3 (en) Nucleic acid binding dyes and uses therefor
WO2009092035A3 (en) Methods and compositions for the analysis of biological molecules
WO2009045344A3 (en) Error-free amplification of dna for clonal sequencing
EP4671381A3 (en) Methods for synthesis and detection of nucleic acids
NZ571352A (en) Separation of nucleic acids from a liquid sample using a liquid flow device
WO2009143500A3 (en) Methods for removing nucleic acid contamination from reagents
WO2009002937A3 (en) Methods for isolating long fragment rna from fixed samples
WO2008127356A3 (en) Methods and kits for isolating cells
WO2008140829A3 (en) Multiplex detection of agricultural pathogens
WO2009153667A3 (en) Method for the detection of ovarian cancer
BR112015008268A2 (en) composition of material, kit, device, cartridge and methods for nucleic acid amplification

Legal Events

Date Code Title Description
121 Ep: the epo has been informed by wipo that ep was designated in this application

Ref document number: 08798912

Country of ref document: EP

Kind code of ref document: A2

NENP Non-entry into the national phase

Ref country code: DE

122 Ep: pct application non-entry in european phase

Ref document number: 08798912

Country of ref document: EP

Kind code of ref document: A2