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WO2009030211A3 - Transglutaminases stables - Google Patents

Transglutaminases stables Download PDF

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Publication number
WO2009030211A3
WO2009030211A3 PCT/DE2008/001463 DE2008001463W WO2009030211A3 WO 2009030211 A3 WO2009030211 A3 WO 2009030211A3 DE 2008001463 W DE2008001463 W DE 2008001463W WO 2009030211 A3 WO2009030211 A3 WO 2009030211A3
Authority
WO
WIPO (PCT)
Prior art keywords
transglutaminases
thermostable
aim
help
resistant
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/DE2008/001463
Other languages
German (de)
English (en)
Other versions
WO2009030211A2 (fr
Inventor
Markus Pietzsch
Thomas Hertel
Christian Marx
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Martin Luther Universitaet Halle Wittenberg
Original Assignee
Martin Luther Universitaet Halle Wittenberg
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Martin Luther Universitaet Halle Wittenberg filed Critical Martin Luther Universitaet Halle Wittenberg
Priority to DE112008003024T priority Critical patent/DE112008003024A5/de
Publication of WO2009030211A2 publication Critical patent/WO2009030211A2/fr
Publication of WO2009030211A3 publication Critical patent/WO2009030211A3/fr
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1025—Acyltransferases (2.3)
    • C12N9/104—Aminoacyltransferases (2.3.2)
    • C12N9/1044—Protein-glutamine gamma-glutamyltransferase (2.3.2.13), i.e. transglutaminase or factor XIII
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y203/00—Acyltransferases (2.3)
    • C12Y203/02—Aminoacyltransferases (2.3.2)
    • C12Y203/02013—Protein-glutamine gamma-glutamyltransferase (2.3.2.13), i.e. transglutaminase or factor XIII

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Peptides Or Proteins (AREA)

Abstract

L'invention concerne des transglutaminases utilisées entre autres pour la réticulation covalente de protéines. On connaît une transglutaminase microbienne, obtenue à partir de Streptomyces mobaraensis, qui présente un faible niveau de stabilité à des températures plus élevées. La transglutaminase commercialisée sous l'appellation d'Activa WM est utilisée principalement dans la technologie alimentaire. Pour des applications ne relevant pas du secteur alimentaire, il faut utiliser des transglutaminases plus thermostables et plus actives pour des réactions de réticulation effectuées à des températures plus élevées. L'invention vise de ce fait à produire des transglutaminases présentant une meilleure thermostabilité. Des transglutaminases modifiées présentant une meilleure stabilité ont pu être produites, par mutagenèse aléatoire, par amplification en chaîne par polymérase (PCR) sujette à erreurs. Les séquences géniques de ces transglutaminases ont été analysées et la séquence d'aminoacide modifiée correspondante a été dérivée. Les régions présentant une meilleure thermostabilité comparativement aux enzymes connues ont pu être identifiées dans la protéine, au moyen de cette information de séquence et des structures cristallines connues. L'invention s'applique à la technologie alimentaire, au secteur pharmaceutique et cosmétique, ainsi qu'à la synthèse polymère.
PCT/DE2008/001463 2007-09-07 2008-08-30 Transglutaminases stables Ceased WO2009030211A2 (fr)

Priority Applications (1)

Application Number Priority Date Filing Date Title
DE112008003024T DE112008003024A5 (de) 2007-09-07 2008-08-30 Thermostabile Transglutaminasen

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE200710042727 DE102007042727A1 (de) 2007-09-07 2007-09-07 Thermostabile Transglutaminasen
DE102007042727.3 2007-09-07

Publications (2)

Publication Number Publication Date
WO2009030211A2 WO2009030211A2 (fr) 2009-03-12
WO2009030211A3 true WO2009030211A3 (fr) 2009-06-25

Family

ID=40429436

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/DE2008/001463 Ceased WO2009030211A2 (fr) 2007-09-07 2008-08-30 Transglutaminases stables

Country Status (2)

Country Link
DE (2) DE102007042727A1 (fr)
WO (1) WO2009030211A2 (fr)

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20100297726A1 (en) 2008-02-13 2010-11-25 Amano Enzyme Inc. Stabilized transglutaminase and process for production thereof
WO2018004014A1 (fr) * 2016-07-01 2018-01-04 国立大学法人九州大学 Protéine recombinée ayant une activité transglutaminase
CA3080512A1 (fr) * 2017-11-07 2019-05-16 Codexis, Inc. Variants de transglutaminase
US20200370026A1 (en) * 2017-11-30 2020-11-26 Amano Enzyme Inc. Modified transglutaminase
CN115210379A (zh) * 2020-03-03 2022-10-18 天野酶制品株式会社 修饰型转谷氨酰胺酶
CN111593011B (zh) * 2020-07-07 2021-12-24 上海东之汇生物科技有限公司 一种转谷氨酰胺酶生产菌
CN116254244B (zh) * 2023-04-17 2025-04-25 华南理工大学 谷氨酰胺转氨酶突变体及其应用

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1310560A1 (fr) * 2000-08-17 2003-05-14 Ajinomoto Co., Inc. Procede de modification de transglutaminase de micro-organismes (mtg)
WO2007020290A1 (fr) * 2005-08-18 2007-02-22 Novo Nordisk Health Care Ag Variantes de transglutaminase de spécificité améliorée

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE19814860A1 (de) * 1998-04-02 1999-10-07 Fuchsbauer Hans Lothar Bakterielle Transglutaminasen
CN101506233A (zh) * 2006-08-18 2009-08-12 诺沃-诺迪斯克保健股份有限公司 具有提高的特异性的转谷氨酰胺酶变异体

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1310560A1 (fr) * 2000-08-17 2003-05-14 Ajinomoto Co., Inc. Procede de modification de transglutaminase de micro-organismes (mtg)
WO2007020290A1 (fr) * 2005-08-18 2007-02-22 Novo Nordisk Health Care Ag Variantes de transglutaminase de spécificité améliorée

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
EIJSINK VINCENT G H ET AL: "Directed evolution of enzyme stability.", BIOMOLECULAR ENGINEERING JUN 2005, vol. 22, no. 1-3, June 2005 (2005-06-01), pages 21 - 30, XP002523728, ISSN: 1389-0344 *
MARX C K ET AL: "Random mutagenesis of a recombinant microbial transglutaminase for the generation of thermostable and heat-sensitive variants", JOURNAL OF BIOTECHNOLOGY, ELSEVIER SCIENCE PUBLISHERS, AMSTERDAM, NL, vol. 136, no. 3-4, 10 September 2008 (2008-09-10), pages 156 - 162, XP024338589, ISSN: 0168-1656, [retrieved on 20080627] *
MARX ET AL: "Soluble expression of a pro-transglutaminase from Streptomyces mobaraensis in Escherichia coli", ENZYME AND MICROBIAL TECHNOLOGY, STONEHAM, MA, US, vol. 40, no. 6, 29 March 2007 (2007-03-29), pages 1543 - 1550, XP022003981, ISSN: 0141-0229 *

Also Published As

Publication number Publication date
DE112008003024A5 (de) 2010-08-19
WO2009030211A2 (fr) 2009-03-12
DE102007042727A1 (de) 2009-09-24

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