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WO2009022536A1 - 細胞の物性値測定方法及び物性測定装置 - Google Patents

細胞の物性値測定方法及び物性測定装置 Download PDF

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Publication number
WO2009022536A1
WO2009022536A1 PCT/JP2008/063500 JP2008063500W WO2009022536A1 WO 2009022536 A1 WO2009022536 A1 WO 2009022536A1 JP 2008063500 W JP2008063500 W JP 2008063500W WO 2009022536 A1 WO2009022536 A1 WO 2009022536A1
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Prior art keywords
permittivity
cell
physical property
measuring physical
determined
Prior art date
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Ceased
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PCT/JP2008/063500
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English (en)
French (fr)
Inventor
Yoichi Katsumoto
Yoshihito Hayashi
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Sony Corp
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Sony Corp
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Priority to CN200880101833.XA priority Critical patent/CN101772700B/zh
Priority to US12/733,031 priority patent/US8478546B2/en
Priority to EP08791735.7A priority patent/EP2175262A4/en
Publication of WO2009022536A1 publication Critical patent/WO2009022536A1/ja
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

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    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • G01N33/5008—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
    • G01N33/5044—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics involving specific cell types
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/483—Physical analysis of biological material
    • G01N33/487—Physical analysis of biological material of liquid biological material
    • G01N33/48707—Physical analysis of biological material of liquid biological material by electrical means
    • G01N33/48728—Investigating individual cells, e.g. by patch clamp, voltage clamp
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/02—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating impedance
    • G01N27/026—Dielectric impedance spectroscopy

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Immunology (AREA)
  • Hematology (AREA)
  • Physics & Mathematics (AREA)
  • Urology & Nephrology (AREA)
  • Molecular Biology (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • Pathology (AREA)
  • General Physics & Mathematics (AREA)
  • General Health & Medical Sciences (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Cell Biology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Microbiology (AREA)
  • Toxicology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Investigating Or Analyzing Materials By The Use Of Electric Means (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Measurement Of Resistance Or Impedance (AREA)

Abstract

　先ず、測定対象の細胞の形状をモデル化し、その細胞にＡＣ電場が印可されたときの複素誘電率応答を数値解析により求める（ステップＳ１）。その結果に基づいて、膜キャパシタンスＣｍ及び細胞質導電率κｉの値を変化させながら数値計算して、誘電スペクトルを算出する（ステップＳ２）。算出した誘電スペクトルを誘電緩和式に回帰させて、比誘電率上昇Δε及び緩和時間τを求める（ステップＳ３）。次に、（Δε，τ）の（Ｃｍ，κｉ）への依存性を求め、測定対象の細胞の形状に応じた回帰式を作成する（ステップＳ４）。細胞の誘電スペクトルを実測し、その値と回帰式とを比較することで、測定対象の細胞の膜キャパシタンスＣｍ，ｅｘｐ及び細胞質導電率κｉ，ｅｘｐを求める（ステップＳ５）。
PCT/JP2008/063500 2007-08-10 2008-07-28 細胞の物性値測定方法及び物性測定装置 Ceased WO2009022536A1 (ja)

Priority Applications (3)

Application Number Priority Date Filing Date Title
CN200880101833.XA CN101772700B (zh) 2007-08-10 2008-07-28 测量细胞的物理性质值的方法和物理性质测量设备
US12/733,031 US8478546B2 (en) 2007-08-10 2008-07-28 Method for measuring physical property values of a cell
EP08791735.7A EP2175262A4 (en) 2007-08-10 2008-07-28 METHOD FOR MEASURING THE PHYSICAL PROPERTIES OF A CELL AND SYSTEM FOR MEASURING PHYSICAL PROPERTIES

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
JP2007209108A JP4893526B2 (ja) 2007-08-10 2007-08-10 細胞の物性値測定方法及び物性測定装置
JP2007-209108 2007-08-10

Publications (1)

Publication Number Publication Date
WO2009022536A1 true WO2009022536A1 (ja) 2009-02-19

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PCT/JP2008/063500 Ceased WO2009022536A1 (ja) 2007-08-10 2008-07-28 細胞の物性値測定方法及び物性測定装置

Country Status (5)

Country Link
US (1) US8478546B2 (ja)
EP (1) EP2175262A4 (ja)
JP (1) JP4893526B2 (ja)
CN (1) CN101772700B (ja)
WO (1) WO2009022536A1 (ja)

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2010079844A1 (ja) * 2009-01-09 2010-07-15 ソニー株式会社 流路デバイス、複素誘電率測定装置及び誘電サイトメトリー装置
CN101975898A (zh) * 2010-09-17 2011-02-16 西安交通大学 一种利用超声脉冲动电研究细胞介电特性的装置

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JP5098817B2 (ja) 2008-05-29 2012-12-12 ソニー株式会社 物性測定装置及び物性測定方法
KR101119381B1 (ko) * 2009-06-18 2012-03-07 한국과학기술원 세포 특성 측정 장치 및 시스템과 이를 이용한 세포 특성 측정 방법
JP5556144B2 (ja) * 2009-11-26 2014-07-23 ソニー株式会社 血球細胞分析装置、血球細胞分析方法及び血球細胞分析システム
JP5549484B2 (ja) * 2010-09-01 2014-07-16 ソニー株式会社 液体試料の電気特性測定のためのサンプルカートリッジと装置
JP5617532B2 (ja) * 2010-10-29 2014-11-05 ソニー株式会社 誘電サイトメトリ装置及び誘電サイトメトリによる細胞分取方法
JP5824950B2 (ja) * 2011-08-08 2015-12-02 ソニー株式会社 血液分析装置および血液分析方法
WO2014038298A1 (ja) * 2012-09-04 2014-03-13 ソニー株式会社 細胞判別装置、細胞判別システム、細胞判別方法及び細胞判別用プログラム
JP2014115256A (ja) 2012-12-12 2014-06-26 Sony Corp 電気的測定用容器、並びに電気的測定用装置および電気的測定方法
CN108931632A (zh) 2013-01-28 2018-12-04 索尼公司 电测量容器、阻抗测量装置及其操作方法
CN105008906B (zh) 2013-03-13 2020-01-07 索尼公司 血液状态分析装置、血液状态分析系统
JP6421749B2 (ja) 2013-03-15 2018-11-14 ソニー株式会社 血液状態解析装置、血液状態解析システム、血液状態解析方法、および該方法をコンピューターに実現させるための血液状態解析プログラム
WO2014156370A1 (ja) 2013-03-29 2014-10-02 ソニー株式会社 血液状態評価装置、血液状態評価システム、血液状態評価方法及びプログラム
US10948478B2 (en) 2013-03-29 2021-03-16 Sony Corporation Blood state analysis device, blood state analysis system, blood state analysis method, and program
JP6569209B2 (ja) 2014-01-07 2019-09-04 ソニー株式会社 電気的測定用カートリッジ、並びに電気的測定装置及び電気的測定方法
JP2015169432A (ja) 2014-03-04 2015-09-28 ソニー株式会社 電気的測定用カートリッジ、並びに電気的測定装置及び電気的測定方法
JP6515677B2 (ja) 2014-07-24 2019-05-22 ソニー株式会社 コンタクト構造体、及び該コンタクト構造体を用いた生体試料用電気的測定装置
JP2016024161A (ja) 2014-07-24 2016-02-08 ソニー株式会社 電気的測定用カートリッジ、生体試料用電気的測定装置、生体試料用電気的測定システム及び生体試料用電気的測定方法
CN107209134B (zh) 2015-02-20 2020-05-26 索尼公司 电特性测量设备、电特性测量系统、电特性测量方法以及用于使计算机实现所述方法的电特性测量程序
RU2730438C2 (ru) 2016-05-11 2020-08-21 Нова Биомедикэл Корпорэйшн Датчик SO2 цельной крови
US10758151B2 (en) * 2017-01-23 2020-09-01 NovaScan, Inc. Techniques for detecting cancerous cells in excised tissue samples using impedance detection
JP6876911B2 (ja) 2017-02-21 2021-05-26 ソニーグループ株式会社 電気的測定用カートリッジ、電気的測定装置及び電気的測定方法
KR102221953B1 (ko) * 2018-08-31 2021-03-04 연세대학교 원주산학협력단 유전영동 힘 인가변화 조절에 따른 세포의 전기분극 특성 분석법
JP6705550B1 (ja) * 2019-03-22 2020-06-03 Toto株式会社 静電チャック
US12019040B2 (en) 2019-09-30 2024-06-25 Cornell University System and devices for monitoring cell-containing materials and methods of their use

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See also references of EP2175262A4

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2010079844A1 (ja) * 2009-01-09 2010-07-15 ソニー株式会社 流路デバイス、複素誘電率測定装置及び誘電サイトメトリー装置
JP2010181399A (ja) * 2009-01-09 2010-08-19 Sony Corp 流路デバイス、複素誘電率測定装置及び誘電サイトメトリー装置
US10222314B2 (en) 2009-01-09 2019-03-05 Sony Corporation Flow channel device, complex permittivity measuring apparatus, and dielectric cytometry system
CN101975898A (zh) * 2010-09-17 2011-02-16 西安交通大学 一种利用超声脉冲动电研究细胞介电特性的装置

Also Published As

Publication number Publication date
JP4893526B2 (ja) 2012-03-07
CN101772700A (zh) 2010-07-07
CN101772700B (zh) 2012-05-02
EP2175262A1 (en) 2010-04-14
US8478546B2 (en) 2013-07-02
US20100136606A1 (en) 2010-06-03
JP2009042141A (ja) 2009-02-26
EP2175262A4 (en) 2014-12-17

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