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WO2009000789A1 - Amélioration du rendement de plantes par modulation du gène myb-ada2 du maïs - Google Patents

Amélioration du rendement de plantes par modulation du gène myb-ada2 du maïs Download PDF

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Publication number
WO2009000789A1
WO2009000789A1 PCT/EP2008/057926 EP2008057926W WO2009000789A1 WO 2009000789 A1 WO2009000789 A1 WO 2009000789A1 EP 2008057926 W EP2008057926 W EP 2008057926W WO 2009000789 A1 WO2009000789 A1 WO 2009000789A1
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WO
WIPO (PCT)
Prior art keywords
plant
zmada2
polypeptide
seq
sequence
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Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/EP2008/057926
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English (en)
Inventor
Wesley Bruce
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CropDesign NV
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CropDesign NV
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Filing date
Publication date
Application filed by CropDesign NV filed Critical CropDesign NV
Publication of WO2009000789A1 publication Critical patent/WO2009000789A1/fr
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/415—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from plants
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
    • Y02A40/10—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
    • Y02A40/146—Genetically Modified [GMO] plants, e.g. transgenic plants

Definitions

  • a "ZmADA2" or “ZmADA2 sequence” comprises a polynucleotide encoding or a polypeptide having the ZmADA2 zinc finger domain, a SANT domain, and/or a SWIRM domain or a biologically active variant or fragment of the ZmADA2 zinc finger domain, a SANT domain, and/or a SWIRM domain. See, for example, Jurata and Gill (1997) MoI Cell Biol 17:5688-98; and Franks, et al., (2002) Development * ⁇ 29:253-63.
  • Polynucleotides that are fragments of a ZmADA2 nucleotide sequence, or a polynucleotide sequence comprising a ZmADA2 zinc finger domain, SANT domain, or SWIRM domain comprise at least 16, 20, 50, 75, 100, 150, 200, 250, 300, 350, 400, 450, 500, 550, 600, 650, 700, 800, 900, 1 ,000, 1 ,100, 1 ,200, 1 ,300 contiguous nucleotides or up to the number of nucleotides present in a full-length ZmADA2 zinc finger domain, SANT domain, SWIRM domain or in a ZmADA2 polynucleotide (i.e., SEQ ID NO: 1 , 1343 nucleotides).
  • Biologically active variants of a ZmADA2 protein of the invention a ZmADA2 zinc finger domain, SANT domain, or SWIRM domain will have at least about 40%, 45%, 50%, 55%, 60%, 65%, 70%, 75%, 80%, 85%, 90%, 91 %, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99% or more sequence identity to the amino acid sequence for the ZmADA2 protein or the consensus ZmADA2 zinc finger domain, SANT domain, or SWIRM domain as determined by sequence alignment programs and parameters described elsewhere herein.
  • determining if a sequence has ZmADA2 activity can be assayed by monitoring histone acetylation in in vitro assays (Balasubramanian, R., et al., (2002) J Biol Chem 277:7989-95).
  • methods to assay for a modulation of transcriptional activity can include monitoring for an alteration in the phenotype of the plant. For example, as discussed in further detail elsewhere herein, modulating the level of a ZmADA2 polypeptide can result in abnormal flower formation or alterations of yield. Methods to assay for these changes are discussed in further detail elsewhere herein.
  • the T m is the temperature (under defined ionic strength and pH) at which 50% of a complementary target sequence hybridizes to a perfectly matched probe. T m is reduced by about 1 °C for each 1% of mismatching; thus, T m , hybridization, and/or wash conditions can be adjusted to hybridize to sequences of the desired identity. For example, if sequences with >90% identity are sought, the T m can be decreased 10 0 C.
  • stringent conditions are selected to be about 5°C lower than the thermal melting point (T m ) for the specific sequence and its complement at a defined ionic strength and pH.
  • Computer implementations of these mathematical algorithms can be utilized for comparison of sequences to determine sequence identity. Such implementations include, but are not limited to: CLUSTAL in the PC/Gene program (available from Intelligenetics, Mountain View, California); the ALIGN program (Version 2.0) and GAP, BESTFIT, BLAST, FASTA, and TFASTA in the GCG Wisconsin Genetics Software Package, Version 10 (available from Accelrys Inc., 9685 Scranton Road, San Diego, California, USA). Alignments using these programs can be performed using the default parameters.
  • the various polynucleotides employed in the methods and compositions of the invention can be provided in expression cassettes for expression in the plant of interest.
  • the cassette will include 5' and 3' regulatory sequences operably linked to a polynucleotide of the invention.
  • "Operably linked" is intended to mean a functional linkage between two or more elements.
  • an operable linkage between a polynucleotide of interest and a regulatory sequence i.e., a promoter
  • Operably linked elements may be contiguous or non-contiguous. When used to refer to the joining of two protein coding regions, by operably linked is intended that the coding regions are in the same reading frame.
  • the polynucleotides of the present invention may also be used in the sense orientation to suppress the expression of endogenous genes in plants.
  • Methods for suppressing gene expression in plants using polynucleotides in the sense orientation are known in the art.
  • the methods generally involve transforming plants with a DNA construct comprising a promoter that drives expression in a plant operably linked to at least a portion of a polynucleotide that corresponds to the transcript of the endogenous gene.
  • a nucleotide sequence has substantial sequence identity to the sequence of the transcript of the endogenous gene, optimally greater than about 65% sequence identity, more optimally greater than about 85% sequence identity, most optimally greater than about 95% sequence identity. See, U.S.
  • the methods and compositions can be used to increase yield in a plant.
  • improved yield means any improvement in the yield of any measured plant product.
  • the improvement in yield can comprise a 0.1%, 0.5%, 1%, 3%, 5%, 10%, 15%, 20%, 30%, 40%, 50%, 60%, 70%, 80%, 90% or greater increase in measured plant product.
  • the increased plant yield can comprise about a 0.5 fold, 1 fold, 2 fold, 4 fold, 8 fold, 16 fold or 32 fold increase in measured plant products.
  • an increase in the bu/acre yield of soybeans or corn derived from a crop having the present treatment as compared with the bu/acre yield from untreated soybeans or corn cultivated under the same conditions would be considered an improved yield.
  • increased yield is also intended at least one of an increase in total seed numbers, an increase in total seed weight, an increase in root biomass and an increase in harvest index.
  • Harvest index is defined as the ratio of yield biomass to the total cumulative biomass at harvest.
  • An isolated polynucleotide comprising a nucleotide sequence selected from the group consisting of: (a) the nucleotide sequence set forth in SEQ ID NO: 1 ;
  • nucleotide sequence having at least 90% sequence identity to SEQ ID NO: 1 wherein said nucleotide sequence encodes a polypeptide having ZmADA2 protein activity;
  • nucleotide sequence comprising at least 50 consecutive nucleotides of SEQ ID NO: 1 wherein said nucleotide sequence encodes a polypeptide having ZmADA2 protein activity;
  • the resulting expression vector pGOS2::ZmADA2a was transformed into Agrobacterium tumefaciens strain LBA4044 and subsequently into Oryza sativa var. Nipponbare plants (see, Chan, et al., (1993) Plant MoI Biol 22(3):491-506, and Chan, et al., (1992) Plant Cell Physiol 33(5):577-583). Transformed rice plants were grown and examined for various growth characteristics as described herein in Example 4.
  • the plates with callus are incubated in the dark at 27-28°C for 3-5 days. Prior to bombardment, the filters with callus are transferred to CM supplemented with 0.25 M mannitol and 0.25 M sorbitol for 3 hr in the dark. The petri dish lids are then left ajar for 20-45 minutes in a sterile hood to allow moisture on tissue to dissipate.
  • Plants were also passed through a root-imaging system that digitally photographs the root morphology and mass from the base of the clear-bottom pots. Plant above-ground area and root mass were determined by counting the total number of pixels from plant parts discriminated from the background. The above-ground value was averaged for the pictures taken on the same time point from the different angles and was converted to a physical surface value expressed in square mm by calibration. Experiments have shown that the above-ground plant area, which corresponds to the total maximum area, measured this way correlates with the biomass of plant parts above-ground.
  • Soybean cultures are initiated twice each month with 5-7 days between each initiation.
  • SB 103 solid medium (per liter) comprises: 1 pkg. MS salts (GIBCO/BRL - Cat# 1 1 117-066); 1 ml B5 vitamins 1000X stock; 60 g maltose; 750 mg MgCI 2 hexahydrate; pH 5.7; and, 2 g gelrite.

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Molecular Biology (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biophysics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Cell Biology (AREA)
  • Botany (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)

Abstract

L'invention concerne des compositions et des méthodes pour moduler le développement des organes floraux, la formation des feuilles, le phototropisme, la dominance apicale, le développement des fruits et l'initiation des racines, ainsi que pour augmenter le rendement d'une plante. Ces compositions comprennent une séquence ZmADA2. Les compositions de l'invention comprennent des séquences d'acides aminés et des séquences nucléotidiques choisies parmi les séquences SEQ ID NO: 1, 2, 4, 5, 8, 9, 10, 11 et 12, ainsi que des variants et des fragments de celles-ci. Les séquences nucléotidiques codant pour ZmADA2 sont situées dans des constructions d'ADN en vue d'une expression dans une plante d'intérêt. L'invention concerne également des méthodes destinées à moduler le niveau d'une séquence ZmADA2 dans une plante ou une partie d'une plante. Ces méthodes consistent à introduire, dans une plante ou une partie d'une plante, un polynucléotide hétérologue comprenant une séquence ZmADA2 de l'invention. Le niveau du polypeptide ZmADA2 peut être augmenté ou abaissé. Une telle méthode peut permettre d'augmenter le rendement de plantes. Dans un mode de réalisation, elle permet d'augmenter la production de grains dans des céréales.
PCT/EP2008/057926 2007-06-22 2008-06-23 Amélioration du rendement de plantes par modulation du gène myb-ada2 du maïs Ceased WO2009000789A1 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US94560907P 2007-06-22 2007-06-22
US60/945,609 2007-06-22

Publications (1)

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WO2009000789A1 true WO2009000789A1 (fr) 2008-12-31

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AR (1) AR067138A1 (fr)
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Cited By (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2011020746A1 (fr) * 2009-08-19 2011-02-24 Basf Plant Science Company Gmbh Plantes ayant des caractères liés au rendement améliorés et leur procédé de fabrication
EP2574622A1 (fr) * 2007-11-26 2013-04-03 BASF Plant Science GmbH Plantes dotées de caractéristiques de rendement améliorées et procédé de fabrication de celles-ci
WO2013077419A1 (fr) * 2011-11-22 2013-05-30 国立大学法人名古屋大学 Gène possédant une fonction d'augmentation des grains pour des végétaux, et applications de ce gène
US8722072B2 (en) 2010-01-22 2014-05-13 Bayer Intellectual Property Gmbh Acaricidal and/or insecticidal active ingredient combinations
US9265252B2 (en) 2011-08-10 2016-02-23 Bayer Intellectual Property Gmbh Active compound combinations comprising specific tetramic acid derivatives
EP3681899A4 (fr) * 2017-09-14 2022-02-23 Pioneer Hi-Bred International, Inc. Compositions et procédés destinés à modifier la taille des plantes
US12410439B2 (en) 2019-12-17 2025-09-09 Pioneer Hi-Bred International, Inc. Reduced stature maize and mads-box transcription factors

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2000003026A2 (fr) * 1998-07-13 2000-01-20 E.I. Du Pont De Nemours And Company Coactivateurs de transcription
WO2006130156A2 (fr) * 2004-07-14 2006-12-07 Mendel Biotechnology, Inc Polynucleotides de vegetaux destines a un rendement et une qualite ameliores

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2000003026A2 (fr) * 1998-07-13 2000-01-20 E.I. Du Pont De Nemours And Company Coactivateurs de transcription
WO2006130156A2 (fr) * 2004-07-14 2006-12-07 Mendel Biotechnology, Inc Polynucleotides de vegetaux destines a un rendement et une qualite ameliores

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
RIYAZ BHAT ET AL: "Interaction of maize Opaque-2 and the transcriptional co-activators GCN5 and ADA2, in the modulation of transcriptional activity", PLANT MOLECULAR BIOLOGY, KLUWER ACADEMIC PUBLISHERS, DORDRECHT, NL, vol. 55, no. 2, 1 May 2004 (2004-05-01), pages 239 - 252, XP019262491, ISSN: 1573-5028 *
VLACHONASIOS KONSTANTINOS E ET AL: "Disruption mutations of ADA2b and GCN5 transcriptional adaptor genes dramatically affect Arabidopsis growth, development, and gene expression.", PLANT CELL, vol. 15, no. 3, March 2003 (2003-03-01), pages 626 - 638, XP002496617, ISSN: 1040-4651 *

Cited By (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP2574622A1 (fr) * 2007-11-26 2013-04-03 BASF Plant Science GmbH Plantes dotées de caractéristiques de rendement améliorées et procédé de fabrication de celles-ci
US8697948B2 (en) 2007-11-26 2014-04-15 Basf Plant Science Gmbh Plants having enhanced yield-related traits and a method for making the same
WO2011020746A1 (fr) * 2009-08-19 2011-02-24 Basf Plant Science Company Gmbh Plantes ayant des caractères liés au rendement améliorés et leur procédé de fabrication
EP2949661A1 (fr) * 2009-08-19 2015-12-02 BASF Plant Science Company GmbH Installations dotées de caractéristiques de rendement améliorées et procédé de fabrication de celles-ci
US8722072B2 (en) 2010-01-22 2014-05-13 Bayer Intellectual Property Gmbh Acaricidal and/or insecticidal active ingredient combinations
US9265252B2 (en) 2011-08-10 2016-02-23 Bayer Intellectual Property Gmbh Active compound combinations comprising specific tetramic acid derivatives
WO2013077419A1 (fr) * 2011-11-22 2013-05-30 国立大学法人名古屋大学 Gène possédant une fonction d'augmentation des grains pour des végétaux, et applications de ce gène
EP3681899A4 (fr) * 2017-09-14 2022-02-23 Pioneer Hi-Bred International, Inc. Compositions et procédés destinés à modifier la taille des plantes
US12410439B2 (en) 2019-12-17 2025-09-09 Pioneer Hi-Bred International, Inc. Reduced stature maize and mads-box transcription factors

Also Published As

Publication number Publication date
AR067138A1 (es) 2009-09-30

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