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WO2009085574A3 - Extraction d'antigènes par énergie micro-onde dans des solvants non aqueux - Google Patents

Extraction d'antigènes par énergie micro-onde dans des solvants non aqueux Download PDF

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Publication number
WO2009085574A3
WO2009085574A3 PCT/US2008/085834 US2008085834W WO2009085574A3 WO 2009085574 A3 WO2009085574 A3 WO 2009085574A3 US 2008085834 W US2008085834 W US 2008085834W WO 2009085574 A3 WO2009085574 A3 WO 2009085574A3
Authority
WO
WIPO (PCT)
Prior art keywords
antigen retrieval
aqueous solvents
antigen
microwave antigen
retrieval solution
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/US2008/085834
Other languages
English (en)
Other versions
WO2009085574A2 (fr
Inventor
Marc E. Key
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Spring Bioscience Corp
Original Assignee
Spring Bioscience Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Spring Bioscience Corp filed Critical Spring Bioscience Corp
Publication of WO2009085574A2 publication Critical patent/WO2009085574A2/fr
Publication of WO2009085574A3 publication Critical patent/WO2009085574A3/fr
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/44—Sample treatment involving radiation, e.g. heat

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  • Physics & Mathematics (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Sampling And Sample Adjustment (AREA)
  • Investigating Or Analysing Biological Materials (AREA)

Abstract

L'invention concerne des procédés destinés à l'immunohistochimie, permettant d'extraire des antigènes à haute température. Dans des exemples particuliers, le procédé selon l'invention consiste à mettre en contact un échantillon comprenant un antigène qui a été fixé par un agent de réticulation à une solution d'extraction d'antigènes comprenant un solvant non aqueux, et à chauffer la solution d'extraction d'antigènes à une température comprise ente 100° C et 200° C à l'aide d'énergie micro-onde. Dans certains exemples, la solution d'extraction d'antigènes comprend au moins un solvant polaire ou un liquide ionique.
PCT/US2008/085834 2007-12-28 2008-12-08 Extraction d'antigènes par énergie micro-onde dans des solvants non aqueux Ceased WO2009085574A2 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US1741407P 2007-12-28 2007-12-28
US61/017,414 2007-12-28

Publications (2)

Publication Number Publication Date
WO2009085574A2 WO2009085574A2 (fr) 2009-07-09
WO2009085574A3 true WO2009085574A3 (fr) 2009-09-03

Family

ID=40824979

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2008/085834 Ceased WO2009085574A2 (fr) 2007-12-28 2008-12-08 Extraction d'antigènes par énergie micro-onde dans des solvants non aqueux

Country Status (1)

Country Link
WO (1) WO2009085574A2 (fr)

Families Citing this family (16)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9874573B2 (en) 2008-10-02 2018-01-23 Gaia Medical Institute Health test for a broad spectrum of health problems
WO2010040097A1 (fr) * 2008-10-02 2010-04-08 Gaia Medical Institute Examen de santé pour un large éventail de problèmes de santé
US8067241B2 (en) * 2009-08-26 2011-11-29 General Electric Company Method and apparatus for antigen retrieval process
US9714943B2 (en) * 2010-12-30 2017-07-25 University Of South Florida Methods and materials for capture antibody targeted fluorescent in-situ hybridization (CAT-FISH)
WO2013078650A1 (fr) * 2011-11-30 2013-06-06 Wang Xiaoya Liquide de récupération d'antigènes et procédé de récupération d'antigènes
GB201303666D0 (en) * 2013-03-01 2013-04-17 Goldsborough Andrew S Sample fixation and stabilisation
FI126757B (en) * 2014-02-28 2017-05-15 Teknologian Tutkimuskeskus Vtt Oy EUTEXT SOLVENT MIXTURES AND THEIR USE
CN105987840B (zh) * 2016-06-03 2018-09-28 浙江世纪康大医疗科技股份有限公司 一种柠檬酸抗原修复液
CN105987839B (zh) * 2016-06-03 2018-09-28 浙江世纪康大医疗科技股份有限公司 一种edta-柠檬酸抗原修复液
EP3427829A1 (fr) 2017-07-12 2019-01-16 Lunaphore Technologies SA Procédés d'extraction in situ d'antigènes d'un échantillon biologique et son imagerie
CN112041648A (zh) * 2018-03-14 2020-12-04 泰坦公司 使用深共晶物的温度升降指示器
CN108614059B (zh) * 2018-06-26 2020-09-22 河南师范大学 疏水性低共熔溶剂涡旋辅助分散液液微萃取-高效液相色谱法检测亚硝酸根的方法
CN111744326B (zh) * 2019-03-26 2022-07-26 天津大学 一种低共熔溶剂及其用于二氧化硫吸收的方法
CN111122760A (zh) * 2019-12-30 2020-05-08 浙江工业大学 基于天然低共熔溶剂预处理-顶空固相微萃取提取植物中挥发性成分的方法
CN111207987A (zh) * 2020-03-13 2020-05-29 东莞市东阳光生物药研发有限公司 一种石蜡包埋三维悬浮细胞团的免疫荧光染色方法
CN113495138A (zh) * 2021-06-22 2021-10-12 南京弗瑞思生物科技有限公司 一种组织切片抗原修复液及其使用方法

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5244787A (en) * 1991-01-31 1993-09-14 Biogenex Laboratories Antigen retrieval in formalin-fixed tissues using microwave energy
WO2002026701A2 (fr) * 2000-09-27 2002-04-04 Scionix Limited Liquides ioniques et leur utilisation comme solvants
WO2006007841A2 (fr) * 2004-07-23 2006-01-26 Dakocytomation Denmark A/S Methodes de recuperation d'antigenes par un traitement a la chaleur et compositions associees

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5244787A (en) * 1991-01-31 1993-09-14 Biogenex Laboratories Antigen retrieval in formalin-fixed tissues using microwave energy
WO2002026701A2 (fr) * 2000-09-27 2002-04-04 Scionix Limited Liquides ioniques et leur utilisation comme solvants
WO2006007841A2 (fr) * 2004-07-23 2006-01-26 Dakocytomation Denmark A/S Methodes de recuperation d'antigenes par un traitement a la chaleur et compositions associees

Also Published As

Publication number Publication date
WO2009085574A2 (fr) 2009-07-09

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