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WO2009064389A3 - Particules d'hydrogel intelligentes ; récolte de biomarqueurs ; purification d'affinité en une étape, exclusion diffusion et protection contre la dégradation - Google Patents

Particules d'hydrogel intelligentes ; récolte de biomarqueurs ; purification d'affinité en une étape, exclusion diffusion et protection contre la dégradation Download PDF

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Publication number
WO2009064389A3
WO2009064389A3 PCT/US2008/012666 US2008012666W WO2009064389A3 WO 2009064389 A3 WO2009064389 A3 WO 2009064389A3 US 2008012666 W US2008012666 W US 2008012666W WO 2009064389 A3 WO2009064389 A3 WO 2009064389A3
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WO
WIPO (PCT)
Prior art keywords
protection against
size exclusion
affinity purification
hydrogel particles
against degradation
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/US2008/012666
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English (en)
Other versions
WO2009064389A2 (fr
Inventor
Alessandra Luchini
David H. Geho
Emanuel Petricoin Iii
Lance A. Liotta
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Individual
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Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
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Publication date
Application filed by Individual filed Critical Individual
Publication of WO2009064389A2 publication Critical patent/WO2009064389A2/fr
Publication of WO2009064389A3 publication Critical patent/WO2009064389A3/fr
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

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Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54313—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals the carrier being characterised by its particulate form
    • G01N33/54346—Nanoparticles

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  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Analytical Chemistry (AREA)
  • Biotechnology (AREA)
  • Nanotechnology (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Cell Biology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Peptides Or Proteins (AREA)
  • Investigating Or Analysing Biological Materials (AREA)

Abstract

L'invention porte sur un procédé pour récolter des biomarqueurs suivant lequel une molécule appât d'affinité est introduite dans du N-isopropylacrylamide pour produire une particule qui effectuera au moins trois fonctions indépendantes en l'espace de quelques minutes. Ceci induit un criblage de dimension moléculaire, une capture d'affinité de toutes les molécules cibles en phase de solution, et une protection complète de protéines récoltées vis-à-vis d'une dégradation enzymatique, les analytes capturés pouvant alors être facilement électro-élués pour analyse.
PCT/US2008/012666 2007-11-09 2008-11-10 Particules d'hydrogel intelligentes ; récolte de biomarqueurs ; purification d'affinité en une étape, exclusion diffusion et protection contre la dégradation Ceased WO2009064389A2 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US98680307P 2007-11-09 2007-11-09
US60/986,803 2007-11-09

Publications (2)

Publication Number Publication Date
WO2009064389A2 WO2009064389A2 (fr) 2009-05-22
WO2009064389A3 true WO2009064389A3 (fr) 2010-03-18

Family

ID=40639377

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2008/012666 Ceased WO2009064389A2 (fr) 2007-11-09 2008-11-10 Particules d'hydrogel intelligentes ; récolte de biomarqueurs ; purification d'affinité en une étape, exclusion diffusion et protection contre la dégradation

Country Status (1)

Country Link
WO (1) WO2009064389A2 (fr)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN114306376B (zh) * 2022-01-04 2023-08-08 北京理工大学 一种花生过敏原蛋白亲和吸附试剂、制备方法及其应用

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2004103351A1 (fr) * 2003-05-21 2004-12-02 The University Of Manchester Particules d'excipient
WO2007012876A1 (fr) * 2005-07-29 2007-02-01 The University Of Manchester Particule d'hydrogel
WO2007038523A2 (fr) * 2005-09-27 2007-04-05 Center For Applied Proteomics And Molecular Medicine Methode d'isolement d'analytes provenant d'un echantillon

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2004103351A1 (fr) * 2003-05-21 2004-12-02 The University Of Manchester Particules d'excipient
WO2007012876A1 (fr) * 2005-07-29 2007-02-01 The University Of Manchester Particule d'hydrogel
WO2007038523A2 (fr) * 2005-09-27 2007-04-05 Center For Applied Proteomics And Molecular Medicine Methode d'isolement d'analytes provenant d'un echantillon

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
JONES ET AL.: "Synthesis and Characterization of Multiresponsive Core-Shell Microgels.", MACROMOLECULES, vol. 33, 2000, pages 8301 - 8306 *
PELTON.: "Temperature-Sensitive Aqueous Microgels.", ADVANCES IN COLLOID AND INTERFACE SCIENCE, vol. 85, 2000, pages 1 - 33 *
WU ET AL.: "The Kinetics of Poly(N-isopropylacrylamide) Microgel Latex Formation.", COLLOID POLYMER SCIENCE, vol. 272, 1994, pages 467 - 477 *

Also Published As

Publication number Publication date
WO2009064389A2 (fr) 2009-05-22

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