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WO2009044868A1 - グルコース・マンノース・キシロース並行発酵性菌およびそれを用いるバイオエタノールの製造方法 - Google Patents

グルコース・マンノース・キシロース並行発酵性菌およびそれを用いるバイオエタノールの製造方法 Download PDF

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Publication number
WO2009044868A1
WO2009044868A1 PCT/JP2008/068073 JP2008068073W WO2009044868A1 WO 2009044868 A1 WO2009044868 A1 WO 2009044868A1 JP 2008068073 W JP2008068073 W JP 2008068073W WO 2009044868 A1 WO2009044868 A1 WO 2009044868A1
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WIPO (PCT)
Prior art keywords
bacterium
mannose
bioethanol
xylose
cellulose
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Ceased
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PCT/JP2008/068073
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English (en)
French (fr)
Inventor
Hideshi Yanase
Kenji Okamoto
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Tottori University NUC
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Tottori University NUC
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Publication date
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Priority to CA2700826A priority Critical patent/CA2700826A1/en
Priority to CN200880119451XA priority patent/CN101889078B/zh
Priority to EP08834910.5A priority patent/EP2206771B1/en
Priority to BRPI0818432-1A2A priority patent/BRPI0818432A2/pt
Priority to US12/681,520 priority patent/US8383377B2/en
Publication of WO2009044868A1 publication Critical patent/WO2009044868A1/ja
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
    • C12P7/04—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
    • C12P7/06—Ethanol, i.e. non-beverage
    • C12P7/08—Ethanol, i.e. non-beverage produced as by-product or from waste or cellulosic material substrate
    • C12P7/10—Ethanol, i.e. non-beverage produced as by-product or from waste or cellulosic material substrate substrate containing cellulosic material
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1022—Transferases (2.) transferring aldehyde or ketonic groups (2.2)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1205—Phosphotransferases with an alcohol group as acceptor (2.7.1), e.g. protein kinases
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/90—Isomerases (5.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
    • C12P7/04—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
    • C12P7/06—Ethanol, i.e. non-beverage
    • C12P7/065—Ethanol, i.e. non-beverage with microorganisms other than yeasts
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
    • Y02E50/00—Technologies for the production of fuel of non-fossil origin
    • Y02E50/10—Biofuels, e.g. bio-diesel

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

　セルロース系、リグノセルロース系バイオマス資源の糖化液を発酵してエタノールを生産できるグルコース・マンノース・キシロース並行発酵性菌を創製し、バイオエタノールの省エネルギー型高効率転換プロセスを構築することを目的として、エシェリヒア・コリ（Escherichia coli）由来のホスホマンノースイソメラーゼをコードする遺伝子を相同組換え法によるダブルクロスオーバーによって染色体上のレバンスクラーゼ遺伝子内に組み込み、かつ、エシェリヒア・コリ由来のキシロースイソメラーゼ、キシルロキナーゼ、トランスアルドラーゼおよびトランスケトラーゼをコードする遺伝子を含有するＤＮＡ断片をベクターに結合させてなる組換えＤＮＡを導入してなるザイモモナス・モビリス（Zymomonas mobilis）、および当該ザイモモナス・モビリスを固定化した系でセルロース系バイオマス資源の糖化液を連続的に発酵させるエタノールの製造方法を提供する。
PCT/JP2008/068073 2007-10-05 2008-10-03 グルコース・マンノース・キシロース並行発酵性菌およびそれを用いるバイオエタノールの製造方法 Ceased WO2009044868A1 (ja)

Priority Applications (5)

Application Number Priority Date Filing Date Title
CA2700826A CA2700826A1 (en) 2007-10-05 2008-10-03 Bacterium capable of fermenting glucose, mannose and xylose simultaneously, and method for production of bioethanol using the bacterium
CN200880119451XA CN101889078B (zh) 2007-10-05 2008-10-03 能够同时发酵葡萄糖、甘露糖和木糖的细菌和使用该细菌生产生物乙醇的方法
EP08834910.5A EP2206771B1 (en) 2007-10-05 2008-10-03 Bacterium capable of fermenting glucose, mannose and xylose simultaneously, and method for production of bioethanol using the bacterium
BRPI0818432-1A2A BRPI0818432A2 (pt) 2007-10-05 2008-10-03 Bactéria fermentativa capaz de fermentar glicose, manose e xilose simultaneamente, e, processo para produzir etanol
US12/681,520 US8383377B2 (en) 2007-10-05 2008-10-03 Bacterium capable of fermenting glucose, mannose and xylose simultaneously, and method for production of bioethanol using the bacterium

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
JP2007261860A JP4124270B1 (ja) 2007-10-05 2007-10-05 グルコース・マンノース・キシロース並行発酵性菌およびそれを用いるバイオエタノールの製造方法
JP2007-261860 2007-10-05

Publications (1)

Publication Number Publication Date
WO2009044868A1 true WO2009044868A1 (ja) 2009-04-09

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PCT/JP2008/068073 Ceased WO2009044868A1 (ja) 2007-10-05 2008-10-03 グルコース・マンノース・キシロース並行発酵性菌およびそれを用いるバイオエタノールの製造方法

Country Status (7)

Country Link
US (1) US8383377B2 (ja)
EP (1) EP2206771B1 (ja)
JP (1) JP4124270B1 (ja)
CN (1) CN101889078B (ja)
BR (1) BRPI0818432A2 (ja)
CA (1) CA2700826A1 (ja)
WO (1) WO2009044868A1 (ja)

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102965297A (zh) * 2012-06-25 2013-03-13 黑龙江省科学院微生物研究所 一株降解纤维素的大肠埃希氏菌
JP2013220398A (ja) * 2012-04-18 2013-10-28 Hitachi Zosen Corp 厨芥類のエタノール発酵における雑菌増殖抑制方法

Families Citing this family (8)

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JPWO2009081941A1 (ja) * 2007-12-25 2011-05-06 国立大学法人鳥取大学 木質系および草本系バイオマス糖化液からのバイオエタノール並行発酵菌
US9428775B2 (en) 2011-02-23 2016-08-30 Macrogen Inc. Transformant for production of lactic acid of high optical purity and method for producing lactic acid using the same
US20130316416A1 (en) * 2012-02-23 2013-11-28 Massachusetts Institute Of Technology Engineering microbes and metabolic pathways for the production of ethylene glycol
CN117904081A (zh) * 2013-07-29 2024-04-19 诺维信公司 蛋白酶变体以及对其进行编码的多核苷酸
CN104131023A (zh) * 2014-07-08 2014-11-05 四川大学 运动发酵单胞菌red重组系统表达质粒及其构建方法与应用
EP3322796A4 (en) 2015-07-13 2019-03-27 Mara Renewables Corporation IMPROVEMENT OF MICROGENONAL FLUID CHANGES OF XYLOSE
EP3208340B1 (en) * 2016-02-22 2020-02-12 versalis S.p.A. Process for propagating a yeast capable to ferment glucose and xylose
AU2020398231B2 (en) * 2019-12-06 2025-09-18 Jacobs Engineering Group, Inc. System and methods for improved anaerobic digestion

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US6566107B1 (en) 2000-11-01 2003-05-20 Midwest Research Institute Recombinant Zymomonas mobilis with improved xylose utilization

Non-Patent Citations (3)

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DIEN BS ET AL.: "Bacteria engineered for fuel ethanol production: current status.", APPL MICROBIOL BIOTECHNOL, vol. 63, no. 3, 2003, pages 258 - 266, XP009133875 *
WEISSER P ET AL.: "Expression of the Escherichia coli pmi gene, encoding phosphomannose-isomerase in Zymomonas mobilis, leads to utilization of mannose as a novel growth substrate, which can be used as a selective marker.", APPL ENVIRON MICROBIOL, vol. 62, no. 11, 1996, pages 4155 - 4161, XP008132392 *

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2013220398A (ja) * 2012-04-18 2013-10-28 Hitachi Zosen Corp 厨芥類のエタノール発酵における雑菌増殖抑制方法
CN102965297A (zh) * 2012-06-25 2013-03-13 黑龙江省科学院微生物研究所 一株降解纤维素的大肠埃希氏菌
CN102965297B (zh) * 2012-06-25 2014-03-12 黑龙江省科学院微生物研究所 一株降解纤维素的大肠埃希氏菌

Also Published As

Publication number Publication date
CA2700826A1 (en) 2009-04-09
EP2206771B1 (en) 2013-04-10
EP2206771A1 (en) 2010-07-14
CN101889078A (zh) 2010-11-17
BRPI0818432A2 (pt) 2014-10-14
JP2010158170A (ja) 2010-07-22
US20100311140A1 (en) 2010-12-09
CN101889078B (zh) 2013-09-11
EP2206771A4 (en) 2010-09-29
JP4124270B1 (ja) 2008-07-23
US8383377B2 (en) 2013-02-26

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