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WO2008136339A1 - 細胞培養方法、細胞培養システム、及び培地調整装置 - Google Patents

細胞培養方法、細胞培養システム、及び培地調整装置 Download PDF

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Publication number
WO2008136339A1
WO2008136339A1 PCT/JP2008/057900 JP2008057900W WO2008136339A1 WO 2008136339 A1 WO2008136339 A1 WO 2008136339A1 JP 2008057900 W JP2008057900 W JP 2008057900W WO 2008136339 A1 WO2008136339 A1 WO 2008136339A1
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cell culture
medium
culture
adjusting
partial pressure
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English (en)
French (fr)
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Satoshi Tanaka
Yoichi Ishizaki
Ryo Suenaga
Yoriko Tokita
Masahito Kogure
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Toyo Seikan Group Holdings Ltd
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Toyo Seikan Kaisha Ltd
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Application filed by Toyo Seikan Kaisha Ltd filed Critical Toyo Seikan Kaisha Ltd
Priority to EP08751999.7A priority Critical patent/EP2141226B2/en
Priority to US12/450,480 priority patent/US9714408B2/en
Priority to CN2008800134564A priority patent/CN101668844B/zh
Publication of WO2008136339A1 publication Critical patent/WO2008136339A1/ja
Anticipated expiration legal-status Critical
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    • C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/30—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration
    • C12M41/32—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration of substances in solution
    • C—CHEMISTRY; METALLURGY
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    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M1/00—Apparatus for enzymology or microbiology
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    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/02—Form or structure of the vessel
    • C12M23/14—Bags
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/26—Constructional details, e.g. recesses, hinges flexible
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M27/00—Means for mixing, agitating or circulating fluids in the vessel
    • C12M27/02—Stirrer or mobile mixing elements
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    • C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
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    • C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
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Abstract

　密封された培養容器での細胞培養において、培養環境が徐々に悪化することを防止する。 　細胞と培地が封入された培養容器に、新たに培地を追加して細胞を培養する細胞培養方法であって、追加する培地のｐＨを細胞の培養に最適なｐＨより高く調整し、及び／又は、追加する培地の溶存二酸化炭素分圧を細胞の培養に最適な圧力より低く調整し、及び／又は、追加する培地の溶存酸素分圧を細胞の培養に最適な圧力より高く調整し、調整した培地と、培養容器内の培地とを混合することで、培養容器内の培地の少なくともｐＨ、溶存二酸化炭素分圧、又は溶存酸素分圧のいずれかを細胞の培養に最適な条件に調整して、培養容器内の細胞を培養する方法とする。
PCT/JP2008/057900 2007-04-27 2008-04-24 細胞培養方法、細胞培養システム、及び培地調整装置 Ceased WO2008136339A1 (ja)

Priority Applications (3)

Application Number Priority Date Filing Date Title
EP08751999.7A EP2141226B2 (en) 2007-04-27 2008-04-24 Cell culture method, cell culture system and medium adjuster
US12/450,480 US9714408B2 (en) 2007-04-27 2008-04-24 Cell culture method
CN2008800134564A CN101668844B (zh) 2007-04-27 2008-04-24 细胞培养方法、细胞培养器械及培养基调整装置

Applications Claiming Priority (2)

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JP2007-119113 2007-04-27
JP2007119113A JP5023795B2 (ja) 2007-04-27 2007-04-27 細胞培養方法、細胞培養システム、及び培地調整装置

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US (1) US9714408B2 (ja)
EP (1) EP2141226B2 (ja)
JP (1) JP5023795B2 (ja)
KR (1) KR101474042B1 (ja)
CN (1) CN101668844B (ja)
WO (1) WO2008136339A1 (ja)

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WO2009123173A1 (ja) * 2008-04-01 2009-10-08 東洋製罐株式会社 培養容器、培養方法、及び培養装置
WO2010103748A1 (ja) 2009-03-09 2010-09-16 東洋製罐株式会社 細胞培養方法、細胞培養装置、容器内の計数対象物の計数方法、及び計数用装置
JP2011239734A (ja) * 2010-05-19 2011-12-01 Toyo Seikan Kaisha Ltd 細胞培養方法、細胞培養装置、及び細胞培養装置の制御方法
JPWO2020004406A1 (ja) * 2018-06-27 2021-07-08 日本曹達株式会社 培養装置、培養対象回収方法、および担体押圧ローラ
EP4245839A1 (en) * 2022-03-14 2023-09-20 Fluigent Method for monitoring at least one substance produced or consumed by a living entity

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CN101668843A (zh) * 2007-04-27 2010-03-10 东洋制罐株式会社 细胞培养装置、细胞培养体系及细胞培养方法
JP5496488B2 (ja) 2008-10-22 2014-05-21 学校法人東京女子医科大学 細胞パターン回収ツール
CN102443534B (zh) * 2011-10-14 2013-12-25 天津开发区合普工贸有限公司 细胞间断缺氧模拟实验设备
KR101909000B1 (ko) * 2012-02-01 2018-10-17 도요세이칸 그룹 홀딩스 가부시키가이샤 세포 배양용 키트 및 세포 배양용 키트의 사용 방법
KR20140107672A (ko) * 2012-02-01 2014-09-04 도요세이칸 그룹 홀딩스 가부시키가이샤 세포 회수 방법
CN112400900A (zh) 2012-03-09 2021-02-26 韦斯塔隆公司 毒肽产生、植物中的肽表达和富含半胱氨酸的肽的组合
KR101508007B1 (ko) 2013-05-31 2015-04-07 재단법인대구경북과학기술원 엘라스틴 유사 인공세포외 기질에 형성된 3차원 세포 군집체를 이용한 체외독성시험방법
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JP6427872B2 (ja) * 2013-12-18 2018-11-28 東洋製罐グループホールディングス株式会社 細胞培養方法、及び細胞培養装置
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Cited By (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2009123173A1 (ja) * 2008-04-01 2009-10-08 東洋製罐株式会社 培養容器、培養方法、及び培養装置
WO2010103748A1 (ja) 2009-03-09 2010-09-16 東洋製罐株式会社 細胞培養方法、細胞培養装置、容器内の計数対象物の計数方法、及び計数用装置
EP2772533A2 (en) 2009-03-09 2014-09-03 Toyo Seikan Kaisha, Ltd. Cell culture method, cell culture device, method for counting subject matters to be counted in container and device for counting
JP2011239734A (ja) * 2010-05-19 2011-12-01 Toyo Seikan Kaisha Ltd 細胞培養方法、細胞培養装置、及び細胞培養装置の制御方法
JPWO2020004406A1 (ja) * 2018-06-27 2021-07-08 日本曹達株式会社 培養装置、培養対象回収方法、および担体押圧ローラ
JP7345194B2 (ja) 2018-06-27 2023-09-15 株式会社BrainGild 培養装置、培養対象回収方法、および担体押圧ローラ
EP4245839A1 (en) * 2022-03-14 2023-09-20 Fluigent Method for monitoring at least one substance produced or consumed by a living entity
WO2023174828A1 (en) * 2022-03-14 2023-09-21 Fluigent Method for monitoring at least one substance produced or consumed by a living entity

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Publication number Publication date
CN101668844A (zh) 2010-03-10
CN101668844B (zh) 2013-12-18
KR101474042B1 (ko) 2014-12-24
JP2008271850A (ja) 2008-11-13
EP2141226A4 (en) 2013-06-12
JP5023795B2 (ja) 2012-09-12
EP2141226B1 (en) 2018-01-03
KR20100015668A (ko) 2010-02-12
US20100062530A1 (en) 2010-03-11
EP2141226B2 (en) 2020-11-11
US9714408B2 (en) 2017-07-25
EP2141226A1 (en) 2010-01-06

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