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WO2008136183A1 - 皮膚または歯肉の軟組織の欠損を補修するための組成物，及び自己線維芽細胞の培養方法 - Google Patents

皮膚または歯肉の軟組織の欠損を補修するための組成物，及び自己線維芽細胞の培養方法 Download PDF

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Publication number
WO2008136183A1
WO2008136183A1 PCT/JP2008/001088 JP2008001088W WO2008136183A1 WO 2008136183 A1 WO2008136183 A1 WO 2008136183A1 JP 2008001088 W JP2008001088 W JP 2008001088W WO 2008136183 A1 WO2008136183 A1 WO 2008136183A1
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aggregate
composition
autologous fibroblasts
autologous
skin
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PCT/JP2008/001088
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English (en)
French (fr)
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Yasuyuki Ishizuka
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Applied Cell Biotechnologies Inc
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Applied Cell Biotechnologies Inc
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Priority to JP2008549706A priority Critical patent/JP4347408B2/ja
Priority to US12/597,915 priority patent/US20100124782A1/en
Publication of WO2008136183A1 publication Critical patent/WO2008136183A1/ja
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    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
    • A61L27/36—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix
    • A61L27/38—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix containing added animal cells
    • A61L27/3839—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix containing added animal cells characterised by the site of application in the body
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
    • A61L27/36—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix
    • A61L27/38—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix containing added animal cells
    • A61L27/3804—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix containing added animal cells characterised by specific cells or progenitors thereof, e.g. fibroblasts, connective tissue cells, kidney cells
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
    • A61L27/36—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix
    • A61L27/38—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix containing added animal cells
    • A61L27/3895—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix containing added animal cells using specific culture conditions, e.g. stimulating differentiation of stem cells, pulsatile flow conditions
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
    • A61L27/50—Materials characterised by their function or physical properties, e.g. injectable or lubricating compositions, shape-memory materials, surface modified materials
    • A61L27/60—Materials for use in artificial skin
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0652—Cells of skeletal and connective tissues; Mesenchyme
    • C12N5/0656—Adult fibroblasts
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
    • A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
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    • C12N2500/00—Specific components of cell culture medium
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    • C12N2500/38—Vitamins
    • C—CHEMISTRY; METALLURGY
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    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2533/00—Supports or coatings for cell culture, characterised by material
    • C12N2533/50—Proteins
    • C12N2533/54—Collagen; Gelatin

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  • Cell Biology (AREA)
  • Veterinary Medicine (AREA)
  • Public Health (AREA)
  • Dermatology (AREA)
  • Medicinal Chemistry (AREA)
  • Oral & Maxillofacial Surgery (AREA)
  • Transplantation (AREA)
  • Epidemiology (AREA)
  • Animal Behavior & Ethology (AREA)
  • Botany (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • Rheumatology (AREA)
  • Urology & Nephrology (AREA)
  • Microbiology (AREA)
  • Vascular Medicine (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Developmental Biology & Embryology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Materials For Medical Uses (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)

Abstract

　【課題】　本発明は，糸状に凝集した自己線維芽細胞を製造するための自己線維芽細胞の凝集塊の製造方法，及びそのような方法で製造された凝集塊を提供することを目的とする。また，本発明は，治療箇所に長期間留まることで効果的な治療効果を持続させることができる，皮膚または歯肉の軟組織の欠損を補修するための組成物，又はそのような組成物を具備する注射剤を提供することを目的とする。 　【解決手段】　上記課題は，自己線維芽細胞を，ビタミンＣが添加された培養培地で培養する工程と，自己線維芽細胞を前記自己線維芽細胞により分泌されたコラーゲンを含む細胞外マトリックスにより糸状の凝集塊となるように，回転培養，振とう培養，または傾斜培養のいずれかの培養方法で培養する工程を含む自己線維芽細胞の凝集塊の製造方法，及び製造される凝集塊などにより解決される。 　
PCT/JP2008/001088 2007-04-28 2008-04-25 皮膚または歯肉の軟組織の欠損を補修するための組成物，及び自己線維芽細胞の培養方法 Ceased WO2008136183A1 (ja)

Priority Applications (2)

Application Number Priority Date Filing Date Title
JP2008549706A JP4347408B2 (ja) 2007-04-28 2008-04-25 皮膚または歯肉の軟組織の欠損を補修するための組成物，及び自己線維芽細胞の培養方法
US12/597,915 US20100124782A1 (en) 2007-04-28 2008-04-25 Composition For Repairing Defect In Skin Or Gingival Soft Tissue And Method Of Culturing Autologous Fibroblasts

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
JP2007-120371 2007-04-28
JP2007120371 2007-04-28

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WO2008136183A1 true WO2008136183A1 (ja) 2008-11-13

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PCT/JP2008/001088 Ceased WO2008136183A1 (ja) 2007-04-28 2008-04-25 皮膚または歯肉の軟組織の欠損を補修するための組成物，及び自己線維芽細胞の培養方法

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US (1) US20100124782A1 (ja)
JP (1) JP4347408B2 (ja)
WO (1) WO2008136183A1 (ja)

Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
RU2380106C1 (ru) * 2009-01-20 2010-01-27 Владимир Валентинович Давыденко Способ коррекции возрастных изменений кожи
JP2016135102A (ja) * 2014-12-22 2016-07-28 ザ チャイニーズ ユニバーシティ オブ ホンコン 体細胞から多能性幹細胞を作製するための機械的操作とプログラミンの併用
JP2017176025A (ja) * 2016-03-30 2017-10-05 国立大学法人 岡山大学 三次元細胞培養物の製造方法
WO2020111271A1 (ja) * 2018-11-30 2020-06-04 株式会社 資生堂 皮膚の色素沈着を治療または予防するための組成物
US10676720B2 (en) 2014-12-22 2020-06-09 The Chinese University Of Hong Kong Combinational use of mechanical manipulation and programin derivatives to increase Oct4, Sox2, Nanog or c-Myc expression in fibroblasts

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103534346A (zh) * 2011-04-21 2014-01-22 细胞移植组织工程有限公司 细胞合成颗粒
US20150110750A1 (en) * 2012-04-30 2015-04-23 The John Hopkins University Methods for using autologous fibroblasts to alter skin identity
JP6813923B1 (ja) * 2020-06-16 2021-01-13 バイオチューブ株式会社 体性幹細胞集積組織構造体及びその製造器具

Citations (3)

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JP2004105742A (ja) * 1995-07-28 2004-04-08 Isolagen Technologies Inc 皮膚および軟組織の欠損修復のための自己皮膚繊維芽細胞の使用
JP2005023080A (ja) * 2003-07-03 2005-01-27 Merck Patent Gmbh 組成物を調製することを目的とする、バウヒニアの水性または水性アルコールエキスの使用
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JP2006510399A (ja) * 2002-11-21 2006-03-30 イソラゲン テクノロジーズ，インコーポレーテッド 未分化間葉細胞を用いた組織の治療
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Cited By (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
RU2380106C1 (ru) * 2009-01-20 2010-01-27 Владимир Валентинович Давыденко Способ коррекции возрастных изменений кожи
JP2016135102A (ja) * 2014-12-22 2016-07-28 ザ チャイニーズ ユニバーシティ オブ ホンコン 体細胞から多能性幹細胞を作製するための機械的操作とプログラミンの併用
US10676720B2 (en) 2014-12-22 2020-06-09 The Chinese University Of Hong Kong Combinational use of mechanical manipulation and programin derivatives to increase Oct4, Sox2, Nanog or c-Myc expression in fibroblasts
JP2017176025A (ja) * 2016-03-30 2017-10-05 国立大学法人 岡山大学 三次元細胞培養物の製造方法
WO2020111271A1 (ja) * 2018-11-30 2020-06-04 株式会社 資生堂 皮膚の色素沈着を治療または予防するための組成物
CN113164783A (zh) * 2018-11-30 2021-07-23 株式会社资生堂 用于治疗或预防皮肤的色素沉积的组合物
JPWO2020111271A1 (ja) * 2018-11-30 2021-10-14 株式会社 資生堂 皮膚の色素沈着を治療または予防するための組成物
JP7495354B2 (ja) 2018-11-30 2024-06-04 株式会社 資生堂 皮膚の色素沈着を治療または予防するための組成物
US12357560B2 (en) 2018-11-30 2025-07-15 Shiseido Company, Ltd. Composition for treating or preventing skin pigmentation

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JP4347408B2 (ja) 2009-10-21
JPWO2008136183A1 (ja) 2010-07-29
US20100124782A1 (en) 2010-05-20

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