[go: up one dir, main page]

WO2008134069A3 - Agents comportant un ligand ancré - Google Patents

Agents comportant un ligand ancré Download PDF

Info

Publication number
WO2008134069A3
WO2008134069A3 PCT/US2008/005528 US2008005528W WO2008134069A3 WO 2008134069 A3 WO2008134069 A3 WO 2008134069A3 US 2008005528 W US2008005528 W US 2008005528W WO 2008134069 A3 WO2008134069 A3 WO 2008134069A3
Authority
WO
WIPO (PCT)
Prior art keywords
ligand
target
target molecule
binding
active site
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/US2008/005528
Other languages
English (en)
Other versions
WO2008134069A2 (fr
Inventor
Weihong Tan
Zehui Cao
Youngmi Kim
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
University of Florida
University of Florida Research Foundation Inc
Original Assignee
University of Florida
University of Florida Research Foundation Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by University of Florida, University of Florida Research Foundation Inc filed Critical University of Florida
Publication of WO2008134069A2 publication Critical patent/WO2008134069A2/fr
Publication of WO2008134069A3 publication Critical patent/WO2008134069A3/fr
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/115—Aptamers, i.e. nucleic acids binding a target molecule specifically and with high affinity without hybridising therewith ; Nucleic acids binding to non-nucleic acids, e.g. aptamers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/16—Aptamers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/30—Chemical structure
    • C12N2310/35—Nature of the modification
    • C12N2310/351—Conjugate
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2320/00—Applications; Uses
    • C12N2320/50—Methods for regulating/modulating their activity

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Molecular Biology (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)

Abstract

L'invention concerne de nouveaux agents thérapeutiques comprenant des molécules de ligand telles que des aptamères, liées à un élément d'ancrage moléculaire (MA). En l'absence du MA, les ligands se diffusent rapidement dans une solution principale après s'être dissociés d'une molécule cible. Grâce au MA lié étroitement à la molécule cible, les molécules à ligand ancré de l'invention restent à proximité étroite du site actif de la molécule cible, même après s'être dissociées de la cible, ce qui permet au ligand de se lier à nouveau rapidement à sa cible. La réversibilité de la liaison au site actif peut être obtenue efficacement et rapidement au moyen d'antidotes comprenant les séquences d'ADN complémentaires. Dans une forme de réalisation préférée, l'invention concerne un agent anticoagulant très efficace dont l'efficacité de liaison à la thrombine est multipliée par rapport à un ligand correspondant à base d'aptamère non ancré.
PCT/US2008/005528 2007-04-26 2008-04-28 Agents comportant un ligand ancré Ceased WO2008134069A2 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US91424207P 2007-04-26 2007-04-26
US60/914,242 2007-04-26

Publications (2)

Publication Number Publication Date
WO2008134069A2 WO2008134069A2 (fr) 2008-11-06
WO2008134069A3 true WO2008134069A3 (fr) 2009-02-12

Family

ID=39926277

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2008/005528 Ceased WO2008134069A2 (fr) 2007-04-26 2008-04-28 Agents comportant un ligand ancré

Country Status (1)

Country Link
WO (1) WO2008134069A2 (fr)

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5756291A (en) * 1992-08-21 1998-05-26 Gilead Sciences, Inc. Aptamers specific for biomolecules and methods of making
US20020037506A1 (en) * 2000-04-18 2002-03-28 Yun Lin Aptamer based two-site binding assay
US20030175703A1 (en) * 2000-09-26 2003-09-18 Sullenger Bruce A. RNA aptamers and methods for identifying the same
US20050123936A1 (en) * 2003-01-16 2005-06-09 Ansari Aseem Z. Method, composition, and kit to design, evaluate, and/or test compounds that modulate regulatory factor binding to nucleic acids
WO2007025049A2 (fr) * 2005-08-26 2007-03-01 Archemix Corp. Aptameres se liant a la thrombine avec une forte affinite

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5756291A (en) * 1992-08-21 1998-05-26 Gilead Sciences, Inc. Aptamers specific for biomolecules and methods of making
US20020037506A1 (en) * 2000-04-18 2002-03-28 Yun Lin Aptamer based two-site binding assay
US20030175703A1 (en) * 2000-09-26 2003-09-18 Sullenger Bruce A. RNA aptamers and methods for identifying the same
US20050123936A1 (en) * 2003-01-16 2005-06-09 Ansari Aseem Z. Method, composition, and kit to design, evaluate, and/or test compounds that modulate regulatory factor binding to nucleic acids
WO2007025049A2 (fr) * 2005-08-26 2007-03-01 Archemix Corp. Aptameres se liant a la thrombine avec une forte affinite

Also Published As

Publication number Publication date
WO2008134069A2 (fr) 2008-11-06

Similar Documents

Publication Publication Date Title
PH12018501850A1 (en) Antigen-binding molecule capable of binding to two or more antigen molecules repeatedly
MX2009005949A (es) Plegamiento de union de oligonucleotido/oligosacarido (ob) usado como estructura para diseño de nuevos enlaces especificos.
WO2007094005A3 (fr) Molécules et leurs procédés d'utilisation pour traiter des maladies associées au ccr5 et aux ligands du ccr5
WO2009111508A3 (fr) Protéines de liaison à la métalloprotéinase 9 et à la métalloprotéinase 2
WO2008083327A3 (fr) Enrichissement à travers des molécules hétéroduplex
IL196732A0 (en) Antigen binding molecules that bind egfr, vectors encoding same, and uses thereof
EA201600338A1 (ru) Биспецифическая связывающая молекула, связывающаяся с vegf и ang2
IN2015DN00255A (fr)
IL197061A (en) Compounds that inhibit the interaction of bcl proteins with binding partners, their medicinal products and their use
DK2185574T3 (da) Antistoffer og relaterede molekyler, der bindes til 24P4C12-proteiner
WO2005113601A8 (fr) Anticorps et molecules derivees de ceux-ci se liant aux proteines steap-1
WO2009136352A8 (fr) Composés anti-angiogéniques
WO2012035050A3 (fr) Variants du facteur viii à absorption cellulaire réduite
WO2010040772A3 (fr) Construction de ciblage comprenant un anticorps anti-polymere et des liposomes ou des microvesicules se liant à ceux-ci
WO2012162547A3 (fr) Conjugués de deux hormones longue durée
WO2011028883A3 (fr) Protéines de liaison à la métalloprotéinase 9 et à la métalloprotéinase 2
WO2008122088A8 (fr) Procédés permettant la détection d'une séquence nucléotidique cible dans un échantillon et utilisant un complexe nucléase/aptamère
WO2009097397A3 (fr) Protéines de liaison à la métalloprotéinase
WO2008134069A3 (fr) Agents comportant un ligand ancré
WO2007008072A3 (fr) Composes de liaison de structures beta-croisees
WO2008007960A3 (fr) Molécules se liant spécifiquement à gainac et leurs utilisations
WO2007026972A3 (fr) Molecule de proteine de liaison
WO2013059439A3 (fr) Polythérapie comprenant une protéine de liaison à la mmp-14
WO2005000870A3 (fr) Peptides ny-eso-1 isoles se liant a des molecules hla de classe ii et leurs utilisations
WO2008124134A3 (fr) Repliement haute pression de protéines en présence de partenaires de liaison

Legal Events

Date Code Title Description
121 Ep: the epo has been informed by wipo that ep was designated in this application

Ref document number: 08743408

Country of ref document: EP

Kind code of ref document: A2

NENP Non-entry into the national phase

Ref country code: DE

122 Ep: pct application non-entry in european phase

Ref document number: 08743408

Country of ref document: EP

Kind code of ref document: A2