[go: up one dir, main page]

WO2008128360A1 - Espace utile présentant des moyens de suppression des germes étrangers - Google Patents

Espace utile présentant des moyens de suppression des germes étrangers Download PDF

Info

Publication number
WO2008128360A1
WO2008128360A1 PCT/CH2007/000191 CH2007000191W WO2008128360A1 WO 2008128360 A1 WO2008128360 A1 WO 2008128360A1 CH 2007000191 W CH2007000191 W CH 2007000191W WO 2008128360 A1 WO2008128360 A1 WO 2008128360A1
Authority
WO
WIPO (PCT)
Prior art keywords
copper
space
subspace
alloy
chamber
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/CH2007/000191
Other languages
German (de)
English (en)
Inventor
Ernst LÖBACH
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
LiCONiC AG
Original Assignee
LiCONiC AG
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by LiCONiC AG filed Critical LiCONiC AG
Priority to PCT/CH2007/000191 priority Critical patent/WO2008128360A1/fr
Publication of WO2008128360A1 publication Critical patent/WO2008128360A1/fr
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

Links

Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L1/00—Enclosures; Chambers
    • B01L1/02—Air-pressure chambers; Air-locks therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/12—Means for regulation, monitoring, measurement or control, e.g. flow regulation of temperature
    • C12M41/14—Incubators; Climatic chambers

Definitions

  • the invention relates to a device with a usable space for storing and / or preparing biological samples and / or goods, in particular an air-conditioning cabinet, according to the preamble of the independent. Claims.
  • a typical climatic cabinet has a utility room for receiving biological samples and may be used as an incubator or refrigerator, e.g. in biological laboratories.
  • Br must generally be set to a defined starting state (zero state, "reset") before the input of samples, in particular the Mutzraum, a possible built-in mechanical structures, be free from germs of any kind.
  • the germs intended for storage, cultivation and propagation are transported through special loading hatches or simply manually through the front door into the working space .
  • the media required for storage, cultivation or multiplication of the useful germs are either automatically added to the mutzraum together with the useful germs or later.
  • the biological task exact climatic conditions, especially in zug on temperature, humidity and gas atmosphere, must be given.
  • the biological task requires that only the useful germs thrive or conserve in the working area.
  • other germs in particular harmful germs, in the following referred to as foreign germs, can be introduced during their storage, cultivation and / or propagation, which may have a negative or even destructive effect on the Kutzkaime.
  • the present invention therefore has as its object the obstruction of the propagation of introduced foreign germs and, in the most favorable case, even killing these germs without impairing the useful germs.
  • the foreign germs pass either airborne from the gas phase or object-worn, for example, over the user's hands, sample containers or sample manipulators into the mutzraum. From the intake air, for example, foreign germs are transported airborne through the leaks of the useful space container, in particular at the edges of access openings, and through the access openings themselves, especially during manual or automatic sample changes during the breeding or storage phase.
  • the invention is based on the idea that foreign nuclei must be located either on surfaces or in the air space within the useful space due to random events.
  • the invention is based, in a first aspect of the Gadanke, on killing the airborne foreign germs directly or indirectly. In a second case She is based on this; to kill or at least prevent growth on solid, moist or in liquid sites foreign germs, as far as the Nutzkeirae are not destroyed or disturbed.
  • the device is designed so as to allow sterilization of the air in the working space after the samples have been taken, i. during the presence of the samples in the work space.
  • a sterilization of the air should not affect the simultaneous development of the NutzkeiTne.
  • the UV radiation is preferably shielded from the useful germs located in the useful space (20).
  • the air in the working space 20 is set in circulation and passed completely or partially through a subspace (40), which is penetrated by germicidal ultraviolet radiation.
  • a second aspect of the invention relates to the killing or obstruction of foreign objects in places that are not necessarily reached by the UV radiation, either because they are in design-related niches and columns, or if it is because the foreign nuclei in immediate nearer to be protected useful germs.
  • At least a part of the surfaces of the useful space is designed for the emission of copper ions.
  • this part of the surfaces is made of an alloy comprising pluck and nails.
  • FIG. 1 shows a section through a climatic chamber.
  • Fig. 1 shows a schematic, vertical section through a climatic cabinet 48. He has a Nutsraum 20, in which fittings 25 are provided. This may be, for example, one or more storage shafts for receiving several laboratory samples on top of each other. Further, in the work space 20, e.g. a transport device for manipulating the stored in the internals 25 laboratory samples may be provided. A user door consisting of outside door 27 and inside door 26 allows manual access to the utility room. Additionally or alternatively, an automated door may be provided for access by robot. An example of a corresponding device is e.g. in US Pat. No. 6,478,524.
  • a subspace 40 is further arranged, through which circulates air with a fan 42 from the rest of Mutsraum.
  • the subspace 40 is arranged vertically, a lower air inlet opening 44 and an upper air outlet opening in the area of the fan 42 suit.
  • the climatic chamber is using funds; which are directed to the fact that all in the Nutsraum 20 foreign germs that are still airborne, are intercepted and killed.
  • the curly arrows 22 are the Indicate air movement within the usable space 20 including the subspace 40.
  • the internal air delivery is, as mentioned, forced by a fan 42, which sits at one end of the subspace 40.
  • the air inlet opening 44 at the other end of the subspace 40 and the air outlet opening on the fan 42 are barred or provided with labyrinths, so that no direct light from the lumen of the subspace can get into the rest of the lumen of the work space.
  • an outside air flow 41 is forced by means of fan 39 between the pre-air opening 38 and the exhaust opening 29. This air flow 41 serves to cool a suitable UV emitter, which sits concentrically in the quartz tube 8.
  • the air streams 22 and 41 are completely decoupled and thus the energy exchange between the lumens of 8 and 40 largely, especially because of the very weak thermal conductivity of the quartz glass of 8.
  • the electrical heat loss of the UV lamp 21 can almost completely with the air flow 41 are discharged, except for a rest, which is caught by the temperature control of the climatic chamber.
  • UV lamps 21 are also relatively higher power than previously possible.
  • the glasses of both the tube 8 and the UV emitter 21 have the same spectral transmittance for the characteristic wavelengths of a suitable UV emitter, in particular 254 nm, but not for the ozonogenic UV radiation, so that the air in the partial room 40 is acted upon by germicidal but not ozonogenic radiation.
  • the partial space wall 47 preferably bears transverse webs 45 on the side facing the UV emitter 21, which effect a swirling of the flow 22, such that airborne germs on the inner UV emitter surfaces of the partial space 40 are liable to adhere with great probability.
  • At least a part or surfaces of the subspace 40 are preferably coated with titanium dioxide, in particular titanium oxide particles 46. It is known that titanium dioxide particles have a strong catalytic effect, which kills bacteria of diverse species under the irradiation of UV light, in particular in conjunction with the high humidity in the flowing air 22.
  • the probability of destroying a free air-borne foreign germ with one or more UV light quanta is smaller by orders of magnitude, that is negligible, than that of a foreign germ accommodated on a wall.
  • the foreign nucleation strategy described so far thus provides i.a. to fix the initially airborne introduced Premdkeime as quickly as possible to surfaces that are acted upon by UV light.
  • premi-germs part of the introduced premi-germs will also be accommodated outside the UV-irradiated areas, especially near the beneficial germs, which may not be exposed to UV radiation by intention.
  • another strategy must be pursued.
  • Cu ions copper ions
  • microorganisms such as viruses, bacteria, fungi, etc. have a killing or at least growth-inhibiting effect. vice On the other hand, microorganisms that are resistant to Cu ions are virtually unknown.
  • a probable explanation is the disruption or blockage of the redox systems in the cytochrome complexes of the mitochondrial respiratory chain of the microorganisms by Cu ions in the cell fluid (see E. Strasburger, Textbook of botany, 35th edition, 2002, 3 321).
  • the Cu ions directly attack the mentioned ionic redox couple by electron scavenging.
  • the energy-supplying electron transport in the respiratory chain as a central metabolic function comes to a standstill.
  • Cu-ion supplying surfaces in the utility rooms of climatic cabinets are known per se for a long time.
  • this technique has not prevailed because copper surfaces, are unstable to the changing atmospheric conditions within the mining areas and are passivated after more or less long periods of time ; in the sense that they can donate little or no more Cu ions in an aqueous medium.
  • a closed copper sulfide surface is not at all, a closed copper (II) oxide surface is almost unable to form Cu ions.
  • the storage shafts for receiving the laboratory samples with copper-ion emitting surfaces may be configured or consist at least partially of a copper-nickel alloy.
  • the surfaces mentioned can now be solid or only superficially made of Cu-ion supplying materials.
  • the materials must be chosen so that on the one hand the surfaces are stable, on the other hand they are able to release Cu ions in sufficient quantities. So a compromise between Cu-ion activity and surface passivity has to be found.
  • the said objects or the substrates of the surfaces are made of an alloy comprising copper and nickel, in particular a copper-nickel alloy, with a nickel content of between 10% and 30%.
  • the copper content should be at least 70, but not more than 90%.
  • An object made of this material automatically overlaps normality. aphot or after chemical treatment with a nickel hydroxide layer, which exerts a passivating effect on the surface on the one hand and on the other hand still enough Cu ions from deeper layers delivers.
  • concentration interval of 10% to 30% nickel gives the optimum in terms of the desired compromise mentioned above.
  • the objects made of aluminum can first be treated by conventional anodic oxidation processes and subsequently the conventionally produced oxide layers can be penetrated by means of copper salt solution using alternating current with copper.
  • objects made of aluminum or aluminum alloys in the work space such as e.g. Parts of the internals 25, be provided on its surface with a copper-containing layer.
  • the invention also relates to the method described above for producing these objects.

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Thermal Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Sustainable Development (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Clinical Laboratory Science (AREA)
  • Apparatus For Disinfection Or Sterilisation (AREA)

Abstract

Dans l'espace utile d'un dispositif de conservation/préparation d'échantillons biologiques, il est prévu un système de stérilisation aux UV comportant un émetteur d'UV (21). Cet émetteur d'UV est disposé dans un espace partiel (40), non occupé par les germes utiles, de façon que la lumière UV ne puisse atteindre les germes utiles. Ceci permet une réduction significative des germes transportés par l'air, dans l'air de circulation, même pendant le fonctionnement, établi pour les déterminations, du dispositif. En outre, au moins une partie des surfaces de l'espace utile est en un alliage cuivre-nickel, lequel empêche la croissance des germes étrangers nuisibles sur ces surfaces.
PCT/CH2007/000191 2007-04-20 2007-04-20 Espace utile présentant des moyens de suppression des germes étrangers Ceased WO2008128360A1 (fr)

Priority Applications (1)

Application Number Priority Date Filing Date Title
PCT/CH2007/000191 WO2008128360A1 (fr) 2007-04-20 2007-04-20 Espace utile présentant des moyens de suppression des germes étrangers

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
PCT/CH2007/000191 WO2008128360A1 (fr) 2007-04-20 2007-04-20 Espace utile présentant des moyens de suppression des germes étrangers

Publications (1)

Publication Number Publication Date
WO2008128360A1 true WO2008128360A1 (fr) 2008-10-30

Family

ID=38828476

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/CH2007/000191 Ceased WO2008128360A1 (fr) 2007-04-20 2007-04-20 Espace utile présentant des moyens de suppression des germes étrangers

Country Status (1)

Country Link
WO (1) WO2008128360A1 (fr)

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP2583752A1 (fr) * 2011-10-21 2013-04-24 Yang-Te Hsu Dispositif à flux laminaire
WO2016070971A1 (fr) * 2014-11-05 2016-05-12 Deutsche Sporthochschule Köln Contenant d'expédition et de stockage de cartes de prélèvement de goutte de sang séché

Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US2541083A (en) * 1945-08-25 1951-02-13 Sperry Corp Electroplating on aluminum
JPH11348175A (ja) * 1998-06-05 1999-12-21 Daido Steel Co Ltd 抗菌性金属板およびその製造方法
EP1046705A1 (fr) * 1999-04-23 2000-10-25 Sanyo Electric Co., Ltd. Enceinte pour régulateur d'humidité
WO2001014515A1 (fr) * 1999-08-25 2001-03-01 Spx Corporation Sterilisation aux ultraviolets d'incubateur pour culture cellulaire a atmosphere renfermant du co¿2?
EP1262540A2 (fr) * 1998-09-29 2002-12-04 SANYO ELECTRIC Co., Ltd. Appareil de culture avec lampe de stérilisation
EP1721684A2 (fr) * 2005-05-09 2006-11-15 Liconic Ag Enceinte utilitaire avec dispositif in situ de désinfection

Patent Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US2541083A (en) * 1945-08-25 1951-02-13 Sperry Corp Electroplating on aluminum
JPH11348175A (ja) * 1998-06-05 1999-12-21 Daido Steel Co Ltd 抗菌性金属板およびその製造方法
EP1262540A2 (fr) * 1998-09-29 2002-12-04 SANYO ELECTRIC Co., Ltd. Appareil de culture avec lampe de stérilisation
EP1046705A1 (fr) * 1999-04-23 2000-10-25 Sanyo Electric Co., Ltd. Enceinte pour régulateur d'humidité
WO2001014515A1 (fr) * 1999-08-25 2001-03-01 Spx Corporation Sterilisation aux ultraviolets d'incubateur pour culture cellulaire a atmosphere renfermant du co¿2?
EP1721684A2 (fr) * 2005-05-09 2006-11-15 Liconic Ag Enceinte utilitaire avec dispositif in situ de désinfection

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
DATABASE WPI Week 200010, Derwent World Patents Index; AN 2000-110676, XP002463271 *

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP2583752A1 (fr) * 2011-10-21 2013-04-24 Yang-Te Hsu Dispositif à flux laminaire
WO2016070971A1 (fr) * 2014-11-05 2016-05-12 Deutsche Sporthochschule Köln Contenant d'expédition et de stockage de cartes de prélèvement de goutte de sang séché

Similar Documents

Publication Publication Date Title
DE69905675T2 (de) Kulturvorrichtung mit Sterilisationslampe
AT397202B (de) Verwendung von chlordioxidgas als chemisches sterilisierungsmittel
EP0655002B1 (fr) Objets bactericides et/ou fongicides en matieres plastiques a usage medical
EP2152324B1 (fr) Procédé et dispositif de protection phytosanitaire pour la lutte contre les parasites des plantes à l'aide de radicaux oxydants produits par voie électrolytique, de lumière uvc et de la technologie de pulvérisation électrostatique assistée par air
EP1721684A2 (fr) Enceinte utilitaire avec dispositif in situ de désinfection
EP2235462B1 (fr) Tour de refroidissement à contamination microbienne réduite
WO2017082201A1 (fr) Agent de régulation de micro-organismes et composition acaricide
EP0075566B1 (fr) Procede pour la preparation de corps moules pour l'aseptisation de l'eau et de solutions aqueuses
EP0109352B1 (fr) Procédé pour stériliser des ustensiles, en particulier en matériaux thermolabiles
WO2008128360A1 (fr) Espace utile présentant des moyens de suppression des germes étrangers
DE102004056189C5 (de) Desinfektionseinrichtung für einen Kryostaten
DE60002083T2 (de) Verfahren zum behandeln von kontaminierenden teilchen enthaltenden gasförmigen medien
DE60313944T2 (de) Kühlschrank mit einem Silberteilchen im Nanobereich aufweisenden Fertigbearbeitungsmaterial
DE202012101333U1 (de) Mobiles mehrteiliges Klimasystem
EP1064028B1 (fr) Desinfection de surfaces planes et formant des locaux au moyen de vapeur d'eau
EP2604292B1 (fr) Procédé de nettoyage pour l'espace utile d'une étuve
DE102010031393A1 (de) Vorrichtung und Verfahren zur Kühlung von Nahrungsmittelmaschinen
DE60202979T2 (de) Einstellbarer Speicherbehälter
DE102014214042A1 (de) System zur Wasserdesinfektion
RU2307668C1 (ru) Состав для дегазации и дезинфекции закрытых помещений
DE102020006226B4 (de) Mobile Vorichtung für die Reinigung und Desinfektion von Raumluft, Bausätze für ihren Zusammenbau und ihre Verwendung
CH644087A5 (en) Process and agent for maintaining the sterility of water and aqueous solutions
WO2008128305A1 (fr) Procédé amélioré de décontamination et/ou de désodorisation des systèmes de conditionnement d'air et systèmes de couverture et dispositif de couverture correspondant
DE19618210C2 (de) Verfahren und Einrichtung zum Bekämpfen von Schädlingen
DE19808895A1 (de) Verfahren zur Veränderung der Konzentration von Inhaltsstoffen in einem Gas, insbesondere Raumluft, und Vorrichtung zur Durchführung dieses Verfahrens

Legal Events

Date Code Title Description
121 Ep: the epo has been informed by wipo that ep was designated in this application

Ref document number: 07720088

Country of ref document: EP

Kind code of ref document: A1

NENP Non-entry into the national phase

Ref country code: DE

122 Ep: pct application non-entry in european phase

Ref document number: 07720088

Country of ref document: EP

Kind code of ref document: A1