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WO2008128144A3 - Monoclonal antibody selecting system, and making and using thereof - Google Patents

Monoclonal antibody selecting system, and making and using thereof Download PDF

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Publication number
WO2008128144A3
WO2008128144A3 PCT/US2008/060165 US2008060165W WO2008128144A3 WO 2008128144 A3 WO2008128144 A3 WO 2008128144A3 US 2008060165 W US2008060165 W US 2008060165W WO 2008128144 A3 WO2008128144 A3 WO 2008128144A3
Authority
WO
WIPO (PCT)
Prior art keywords
cells
transgenic
chimeric protein
monoclonal antibody
domain
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/US2008/060165
Other languages
French (fr)
Other versions
WO2008128144A2 (en
Inventor
Shuang Zhang
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Individual
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Individual filed Critical Individual
Publication of WO2008128144A2 publication Critical patent/WO2008128144A2/en
Publication of WO2008128144A3 publication Critical patent/WO2008128144A3/en
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

Links

Classifications

    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
    • A01K67/027—New or modified breeds of vertebrates
    • A01K67/0275—Genetically modified vertebrates, e.g. transgenic
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C12N15/8509—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells for producing genetically modified animals, e.g. transgenic
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2217/00—Genetically modified animals
    • A01K2217/05—Animals comprising random inserted nucleic acids (transgenic)
    • A01K2217/052—Animals comprising random inserted nucleic acids (transgenic) inducing gain of function
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2227/00—Animals characterised by species
    • A01K2227/10—Mammal
    • A01K2227/105—Murine
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2267/00—Animals characterised by purpose
    • A01K2267/01—Animal expressing industrially exogenous proteins
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/40—Fusion polypeptide containing a tag for immunodetection, or an epitope for immunisation

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • Zoology (AREA)
  • Veterinary Medicine (AREA)
  • General Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Chemical & Material Sciences (AREA)
  • Environmental Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Animal Behavior & Ethology (AREA)
  • Molecular Biology (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Biodiversity & Conservation Biology (AREA)
  • Biochemistry (AREA)
  • Animal Husbandry (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

The present invention provides a DNA encoding a chimeric protein that comprises one transmembrane domain and one extracellular Fc-binding domain and provides transgenic myeloma cells and transgenic mice having the DNA integrated into their genome. The invention also provides methods of preparing transgenic myeloma cell and transgenic mice expressing the chimeric protein. The invention further provides methods of using the transgenic myeloma cells and transgenic mice said mice to facilitate isolation of monoclonal antibodies-producing B-cells. Specifically, antibody-producing cells are engineered to express a chimeric protein that comprises a transmembrane domain and an extracellular Protein G' domain, rendering the cells binding to antibodies released by themselves. Such character simplifies the subsequent selection of monoclonal antibody-producing cells.
PCT/US2008/060165 2007-04-15 2008-04-14 Monoclonal antibody selecting system, and making and using thereof Ceased WO2008128144A2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US91188907P 2007-04-15 2007-04-15
US60/911,889 2007-04-15

Publications (2)

Publication Number Publication Date
WO2008128144A2 WO2008128144A2 (en) 2008-10-23
WO2008128144A3 true WO2008128144A3 (en) 2009-02-12

Family

ID=39864636

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2008/060165 Ceased WO2008128144A2 (en) 2007-04-15 2008-04-14 Monoclonal antibody selecting system, and making and using thereof

Country Status (1)

Country Link
WO (1) WO2008128144A2 (en)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8354389B2 (en) 2009-08-14 2013-01-15 Regeneron Pharmaceuticals, Inc. miRNA-regulated differentiation-dependent self-deleting cassette
EP3221455B1 (en) * 2014-11-20 2020-08-12 Yissum Research and Development Company of the Hebrew University of Jerusalem Ltd. Compositions and methods for producing polypeptides with a modified glycosylation pattern in plant cells
CN114134163A (en) * 2021-11-05 2022-03-04 中国农业大学 Single antigen specific transgenic hybridoma cell screening method

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6180343B1 (en) * 1998-10-08 2001-01-30 Rigel Pharmaceuticals, Inc. Green fluorescent protein fusions with random peptides
US20030162947A1 (en) * 2001-02-20 2003-08-28 Meagher Richard B. Rapid production of monoclonal antibodies
US20060228710A1 (en) * 2003-02-14 2006-10-12 Morris David W Novel therapeutic targets in cancer
US7166423B1 (en) * 1992-10-21 2007-01-23 Miltenyi Biotec Gmbh Direct selection of cells by secretion product

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7166423B1 (en) * 1992-10-21 2007-01-23 Miltenyi Biotec Gmbh Direct selection of cells by secretion product
US6180343B1 (en) * 1998-10-08 2001-01-30 Rigel Pharmaceuticals, Inc. Green fluorescent protein fusions with random peptides
US20030162947A1 (en) * 2001-02-20 2003-08-28 Meagher Richard B. Rapid production of monoclonal antibodies
US20060228710A1 (en) * 2003-02-14 2006-10-12 Morris David W Novel therapeutic targets in cancer

Also Published As

Publication number Publication date
WO2008128144A2 (en) 2008-10-23

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