[go: up one dir, main page]

WO2008118998A3 - Procédé de détection multiplex et de quantification d'acides nucléiques - Google Patents

Procédé de détection multiplex et de quantification d'acides nucléiques Download PDF

Info

Publication number
WO2008118998A3
WO2008118998A3 PCT/US2008/058333 US2008058333W WO2008118998A3 WO 2008118998 A3 WO2008118998 A3 WO 2008118998A3 US 2008058333 W US2008058333 W US 2008058333W WO 2008118998 A3 WO2008118998 A3 WO 2008118998A3
Authority
WO
WIPO (PCT)
Prior art keywords
quantitation
nucleic acids
multiplex detection
sample
methods
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/US2008/058333
Other languages
English (en)
Other versions
WO2008118998A2 (fr
Inventor
Vladimir Slepnev
John Unger
Elizabeth Garcia
Kyle Hart
Benjamin Stone
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Primera Biosystems Inc
Original Assignee
Primera Biosystems Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Primera Biosystems Inc filed Critical Primera Biosystems Inc
Publication of WO2008118998A2 publication Critical patent/WO2008118998A2/fr
Publication of WO2008118998A3 publication Critical patent/WO2008118998A3/fr
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6827—Hybridisation assays for detection of mutation or polymorphism
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6862—Ligase chain reaction [LCR]

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

La présente invention concerne des procédés de détection quantitative de séquences cibles dans un échantillon. Lesdits procédés peuvent être utilisés dans la détection multiplex SNP quantitative en vue de déterminer les changements de profils de mutation dans un échantillon au fil du temps. Il peut s'agir par exemple de mutations survenant à la suite d'une intervention thérapeutique, telle qu'une chimiothérapie dans le traitement du cancer.
PCT/US2008/058333 2007-03-27 2008-03-27 Procédé de détection multiplex et de quantification d'acides nucléiques Ceased WO2008118998A2 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US92023307P 2007-03-27 2007-03-27
US60/920,233 2007-03-27

Publications (2)

Publication Number Publication Date
WO2008118998A2 WO2008118998A2 (fr) 2008-10-02
WO2008118998A3 true WO2008118998A3 (fr) 2008-11-27

Family

ID=39713869

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2008/058333 Ceased WO2008118998A2 (fr) 2007-03-27 2008-03-27 Procédé de détection multiplex et de quantification d'acides nucléiques

Country Status (1)

Country Link
WO (1) WO2008118998A2 (fr)

Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US10131951B2 (en) 2010-08-06 2018-11-20 Ariosa Diagnostics, Inc. Assay systems for genetic analysis
US10233496B2 (en) 2010-08-06 2019-03-19 Ariosa Diagnostics, Inc. Ligation-based detection of genetic variants
US10533223B2 (en) 2010-08-06 2020-01-14 Ariosa Diagnostics, Inc. Detection of target nucleic acids using hybridization
US10718019B2 (en) 2011-01-25 2020-07-21 Ariosa Diagnostics, Inc. Risk calculation for evaluation of fetal aneuploidy
US11203786B2 (en) 2010-08-06 2021-12-21 Ariosa Diagnostics, Inc. Detection of target nucleic acids using hybridization

Families Citing this family (18)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP2280079A1 (fr) * 2009-07-31 2011-02-02 Qiagen GmbH Procédé à base de ligature de quantification normalisée d'acides nucléiques
US10167508B2 (en) 2010-08-06 2019-01-01 Ariosa Diagnostics, Inc. Detection of genetic abnormalities
AU2015201176B2 (en) * 2010-08-06 2017-09-07 F. Hoffmann-La Roche Ag Assay systems for determination of source contribution in a sample
US11031095B2 (en) 2010-08-06 2021-06-08 Ariosa Diagnostics, Inc. Assay systems for determination of fetal copy number variation
US20140342940A1 (en) 2011-01-25 2014-11-20 Ariosa Diagnostics, Inc. Detection of Target Nucleic Acids using Hybridization
US20120034603A1 (en) * 2010-08-06 2012-02-09 Tandem Diagnostics, Inc. Ligation-based detection of genetic variants
US11270781B2 (en) 2011-01-25 2022-03-08 Ariosa Diagnostics, Inc. Statistical analysis for non-invasive sex chromosome aneuploidy determination
US9994897B2 (en) 2013-03-08 2018-06-12 Ariosa Diagnostics, Inc. Non-invasive fetal sex determination
US10131947B2 (en) 2011-01-25 2018-11-20 Ariosa Diagnostics, Inc. Noninvasive detection of fetal aneuploidy in egg donor pregnancies
US8712697B2 (en) 2011-09-07 2014-04-29 Ariosa Diagnostics, Inc. Determination of copy number variations using binomial probability calculations
JP6255353B2 (ja) 2012-02-03 2017-12-27 カリフォルニア インスティチュート オブ テクノロジー 多重生化学アッセイにおける信号エンコードおよびデコード
US10289800B2 (en) 2012-05-21 2019-05-14 Ariosa Diagnostics, Inc. Processes for calculating phased fetal genomic sequences
CA2878280A1 (fr) 2012-07-19 2014-01-23 Ariosa Diagnostics, Inc. Detection a base de ligature sequentielle multiplexe de variants genetiques
US9791372B2 (en) 2012-08-03 2017-10-17 California Institute Of Technology Multiplexing and quantification in PCR with reduced hardware and requirements
US20190112636A1 (en) * 2017-10-16 2019-04-18 ChromaCode, Inc. Methods and compositions for nucleic acid detection
CN112424377A (zh) 2018-04-17 2021-02-26 克罗玛科德公司 用于多重分析的方法和系统
US12203129B2 (en) 2018-07-03 2025-01-21 ChromaCode, Inc. Formulations and signal encoding and decoding methods for massively multiplexed biochemical assays
DE102018213027A1 (de) * 2018-08-03 2020-02-06 Robert Bosch Gmbh Reaktionsgemisch, Verfahren und Kit zur Durchführung einer quantitativen Echtzeit-PCR

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1997045559A1 (fr) * 1996-05-29 1997-12-04 Cornell Research Foundation, Inc. Detection de differences dans des sequences d'acides nucleiques utilisant une combinaison de la detection par ligase et de reactions d'amplification en chaine par polymerase
EP1130113A1 (fr) * 2000-02-15 2001-09-05 Johannes Petrus Schouten Méthode d'amplification dépendant de ligatures multiples
EP1319718A1 (fr) * 2001-12-14 2003-06-18 Keygene N.V. Analyse et détection de séquences cibles multiples à haut rendement

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1997045559A1 (fr) * 1996-05-29 1997-12-04 Cornell Research Foundation, Inc. Detection de differences dans des sequences d'acides nucleiques utilisant une combinaison de la detection par ligase et de reactions d'amplification en chaine par polymerase
EP1130113A1 (fr) * 2000-02-15 2001-09-05 Johannes Petrus Schouten Méthode d'amplification dépendant de ligatures multiples
EP1319718A1 (fr) * 2001-12-14 2003-06-18 Keygene N.V. Analyse et détection de séquences cibles multiples à haut rendement

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
BRINSON E C ET AL: "INTRODUCTION TO PCR/OLA/SCS, A MULTIPLEX DNA TEST, AND ITS APPLICATION TO CYSTIC FIBROSIS", GENETIC TESTING, LARCHMONT, NY, US, vol. 1, no. 1, 1 January 1997 (1997-01-01), pages 61 - 68, XP009057884, ISSN: 1090-6576 *

Cited By (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US10131951B2 (en) 2010-08-06 2018-11-20 Ariosa Diagnostics, Inc. Assay systems for genetic analysis
US10233496B2 (en) 2010-08-06 2019-03-19 Ariosa Diagnostics, Inc. Ligation-based detection of genetic variants
US10533223B2 (en) 2010-08-06 2020-01-14 Ariosa Diagnostics, Inc. Detection of target nucleic acids using hybridization
US11203786B2 (en) 2010-08-06 2021-12-21 Ariosa Diagnostics, Inc. Detection of target nucleic acids using hybridization
US10718019B2 (en) 2011-01-25 2020-07-21 Ariosa Diagnostics, Inc. Risk calculation for evaluation of fetal aneuploidy
US10718024B2 (en) 2011-01-25 2020-07-21 Ariosa Diagnostics, Inc. Risk calculation for evaluation of fetal aneuploidy

Also Published As

Publication number Publication date
WO2008118998A2 (fr) 2008-10-02

Similar Documents

Publication Publication Date Title
WO2008118998A3 (fr) Procédé de détection multiplex et de quantification d'acides nucléiques
WO2012094492A3 (fr) Traceurs adn combinatoires et procédés de préparation et utilisation de ceux-ci
WO2008069906A3 (fr) Expression numérisée de l'analyse génétique
HK1213944A1 (zh) 用於靶向基因组分析
MY161966A (en) Methods and genotyping panels for detecting alleles, genomes, and transcriptomes
WO2009151691A3 (fr) Polymorphismes génétiques associés à une thrombose veineuse, leurs procédés de détection et leurs utilisations
WO2009006577A3 (fr) Compositions et méthodes pour inhiber la protéine ezh2
WO2009120374A3 (fr) Procédés et compositions pour la préparation d’échantillon d’acide nucléique
WO2011106541A3 (fr) Méthodes de diagnostic impliquant une perte d'hétérozygosité
WO2010127304A3 (fr) Procédés de séquençage
WO2010018563A3 (fr) Compositions et procédés de pronostic d'un lymphome
ATE533838T1 (de) Verfahren zur verbesserten einführung von dna in bakterienzellen
SI2089343T1 (sl) Klik kemija za proizvodnjo poročevalskih molekul
WO2008088860A3 (fr) Polymorphismes présents dans la voie de signalisation de l'egfr utilisés comme marqueurs pour le traitement du cancer
WO2008087040A3 (fr) Procédés et acides nucléiques pour analyses des troubles prolifératifs cellulaires
WO2008073177A3 (fr) Profils d'expression génique et protéique associés à l'efficacité thérapeutique de l'irinotécan
WO2011153354A8 (fr) Procédés et kits de détection in situ de séquences de nucléotides
WO2009014612A3 (fr) Nucléotides modifiés, procédés pour les préparer et les utiliser
WO2012084173A3 (fr) Méthodes et compositions de détection d'une mutation dans le gène du récepteur du facteur de croissance épidermique humain
EP2208795A4 (fr) Procédé de détection d'acide nucléique, et coffret de détection d'acide nucléique
WO2008122088A8 (fr) Procédés permettant la détection d'une séquence nucléotidique cible dans un échantillon et utilisant un complexe nucléase/aptamère
WO2010065961A3 (fr) Compositions et procédés concernant le mir-31
WO2010009377A3 (fr) Procédés et compositions pour une analyse pharmacogénétique de médicaments anti-inflammatoires dans le traitement d'une arthrite rhumatoïde et d'autres maladies inflammatoires
GB2439437B (en) Method for determining the concentration of nucleic acids
WO2011021177A3 (fr) Compositions et procédés de pronostic et de traitement du cancer de la prostate

Legal Events

Date Code Title Description
121 Ep: the epo has been informed by wipo that ep was designated in this application

Ref document number: 08744418

Country of ref document: EP

Kind code of ref document: A2

NENP Non-entry into the national phase

Ref country code: DE

122 Ep: pct application non-entry in european phase

Ref document number: 08744418

Country of ref document: EP

Kind code of ref document: A2