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WO2008109674A3 - Cell expansion system and methods of use - Google Patents

Cell expansion system and methods of use Download PDF

Info

Publication number
WO2008109674A3
WO2008109674A3 PCT/US2008/055915 US2008055915W WO2008109674A3 WO 2008109674 A3 WO2008109674 A3 WO 2008109674A3 US 2008055915 W US2008055915 W US 2008055915W WO 2008109674 A3 WO2008109674 A3 WO 2008109674A3
Authority
WO
WIPO (PCT)
Prior art keywords
methods
cell expansion
loops
expansion system
hollow fibers
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/US2008/055915
Other languages
French (fr)
Other versions
WO2008109674A2 (en
Inventor
Glen Delbert Antwiler
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Terumo BCT Inc
Original Assignee
Terumo BCT Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Terumo BCT Inc filed Critical Terumo BCT Inc
Priority to CA2680130A priority Critical patent/CA2680130C/en
Priority to EP08731441.5A priority patent/EP2129764B1/en
Priority to JP2009552860A priority patent/JP2010519937A/en
Publication of WO2008109674A2 publication Critical patent/WO2008109674A2/en
Publication of WO2008109674A3 publication Critical patent/WO2008109674A3/en
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0652—Cells of skeletal and connective tissues; Mesenchyme
    • C12N5/0662—Stem cells
    • C12N5/0663—Bone marrow mesenchymal stem cells (BM-MSC)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M25/00—Means for supporting, enclosing or fixing the microorganisms, e.g. immunocoatings
    • C12M25/10—Hollow fibers or tubes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M25/00—Means for supporting, enclosing or fixing the microorganisms, e.g. immunocoatings
    • C12M25/10—Hollow fibers or tubes
    • C12M25/12—Hollow fibers or tubes the culture medium flowing outside the fiber or tube
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
    • C12M29/10—Perfusion
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
    • C12M29/16—Hollow fibers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2521/00—Culture process characterised by the use of hydrostatic pressure, flow or shear forces

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Genetics & Genomics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Sustainable Development (AREA)
  • Immunology (AREA)
  • Developmental Biology & Embryology (AREA)
  • Analytical Chemistry (AREA)
  • Hematology (AREA)
  • Rheumatology (AREA)
  • Cell Biology (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • External Artificial Organs (AREA)
  • Separation Using Semi-Permeable Membranes (AREA)

Abstract

Cell expansion systems and methods of use are provided. The cell expansion systems generally include a hollow fiber cell growth chamber, and first and second circulation loops (intracapillary loops and extracapillary loops) associated with the interior of the hollow fibers and exterior of the hollow fibers, respectively. Detachable flow circuits and methods of expanding cells are also provided.
PCT/US2008/055915 2007-03-05 2008-03-05 Cell expansion system and methods of use Ceased WO2008109674A2 (en)

Priority Applications (3)

Application Number Priority Date Filing Date Title
CA2680130A CA2680130C (en) 2007-03-05 2008-03-05 Cell expansion system and methods of use
EP08731441.5A EP2129764B1 (en) 2007-03-05 2008-03-05 Cell expansion system and methods of use
JP2009552860A JP2010519937A (en) 2007-03-05 2008-03-05 Cell proliferation system and method of use

Applications Claiming Priority (14)

Application Number Priority Date Filing Date Title
US89290807P 2007-03-05 2007-03-05
US89291107P 2007-03-05 2007-03-05
US89296607P 2007-03-05 2007-03-05
US89297707P 2007-03-05 2007-03-05
US60/892,908 2007-03-05
US60/892,977 2007-03-05
US60/892,911 2007-03-05
US60/892,966 2007-03-05
US91139307P 2007-04-12 2007-04-12
US60/911,393 2007-04-12
US91159407P 2007-04-13 2007-04-13
US60/911,594 2007-04-13
US97149407P 2007-09-11 2007-09-11
US60/971,494 2007-09-11

Publications (2)

Publication Number Publication Date
WO2008109674A2 WO2008109674A2 (en) 2008-09-12
WO2008109674A3 true WO2008109674A3 (en) 2009-04-09

Family

ID=39739089

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2008/055915 Ceased WO2008109674A2 (en) 2007-03-05 2008-03-05 Cell expansion system and methods of use

Country Status (5)

Country Link
US (3) US8309347B2 (en)
EP (1) EP2129764B1 (en)
JP (2) JP2010519937A (en)
CA (1) CA2680130C (en)
WO (1) WO2008109674A2 (en)

Families Citing this family (53)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ES2665024T3 (en) * 2007-03-05 2018-04-24 Terumo Bct, Inc. Methods to control cell movement in hollow fiber bioreactors
CA2680130C (en) 2007-03-05 2016-01-12 Caridianbct, Inc. Cell expansion system and methods of use
EP2168668A1 (en) * 2008-09-25 2010-03-31 Gambro Lundia AB Membrane for cell expansion
EP2344620B1 (en) * 2008-10-06 2014-05-28 Mohr, Ulrich Culture/exposure device for cell and/or bacteria cultures
JP5284031B2 (en) * 2008-10-16 2013-09-11 独立行政法人国立循環器病研究センター Cell dispersion method
US9284523B2 (en) 2008-10-27 2016-03-15 Terumo Bct, Inc. Premounted fluid conveyance assembly for cell expansion system and method of use associated therewith
US8399245B2 (en) 2009-02-18 2013-03-19 Terumo Bct, Inc. Rotation system for cell growth chamber of a cell expansion system and method of use therefor
GB0917565D0 (en) * 2009-10-08 2009-11-25 Univ Nottingham An observation cell arrangement
US8512566B2 (en) * 2009-12-11 2013-08-20 General Electric Company Disposable fluid path systems and methods for processing complex biological materials
US9057045B2 (en) 2009-12-29 2015-06-16 Terumo Bct, Inc. Method of loading and distributing cells in a bioreactor of a cell expansion system
EP2556141B1 (en) * 2010-04-09 2019-05-01 Terumo BCT, Inc. Air removal chamber for a cell expansion system and method of use associated therewith
WO2011140231A1 (en) 2010-05-05 2011-11-10 Caridianbct, Inc. Method of reseeding adherent cells grown in a hollow fiber bioreactor system
WO2012048275A2 (en) 2010-10-08 2012-04-12 Caridianbct, Inc. Configurable methods and systems of growing and harvesting cells in a hollow fiber bioreactor system
WO2012158809A2 (en) * 2011-05-17 2012-11-22 Terumo Bct, Inc. Systems and methods for expanding high density non-adherent cells
WO2012168295A1 (en) * 2011-06-06 2012-12-13 ReGenesys BVBA Expansion of stem cells in hollow fiber bioreactors
EP2639294A1 (en) * 2012-03-15 2013-09-18 CellProthera Automaton and automated method for cell culture
CN104583386A (en) * 2012-08-20 2015-04-29 泰尔茂比司特公司 Concentrates components of the fluid circulated through the cell growth chamber
CN110229780A (en) * 2012-08-20 2019-09-13 泰尔茂比司特公司 The method for being loaded in the bioreactor of cell expansion system and distributing cell
US9273278B2 (en) * 2013-01-07 2016-03-01 Cesco Bioengineering Co., Ltd. Large scale cell harvesting method for pack-bed culture device
ES2827250T3 (en) 2013-04-12 2021-05-20 Houston Methodist Hospital Improvement of organs for transplantation
JP6612227B2 (en) * 2013-11-16 2019-11-27 テルモ ビーシーティー、インコーポレーテッド Cell growth in bioreactors
EP3613841B1 (en) * 2014-03-25 2022-04-20 Terumo BCT, Inc. Passive replacement of media
WO2015164808A1 (en) * 2014-04-24 2015-10-29 Terumo Bct, Inc. Measuring flow rate
JP6719387B2 (en) 2014-04-28 2020-07-08 ビババイオセル エスピーエー Automatic cell culture and recovery device
WO2016049421A1 (en) 2014-09-26 2016-03-31 Terumo Bct, Inc. Scheduled feed
US9487748B2 (en) * 2014-11-05 2016-11-08 Palo Alto Research Center Incorporated Dual-compartment bioreactor for use in wastewater treatment and algal production
EP3233280B1 (en) * 2014-12-18 2021-03-17 Global Life Sciences Solutions Operations UK Ltd Devices, methods and systems for collecting waste from a bioreactor
JP6351113B2 (en) * 2015-01-29 2018-07-04 藤森工業株式会社 Shaking culture apparatus and culture method using the same
WO2016183231A1 (en) * 2015-05-12 2016-11-17 Baker Group, LLP Method and system for a bioartificial organ
US10577585B2 (en) * 2015-05-13 2020-03-03 Terumo Bct, Inc. Cell expansion
WO2017004592A1 (en) 2015-07-02 2017-01-05 Terumo Bct, Inc. Cell growth with mechanical stimuli
JP6930068B2 (en) * 2016-03-30 2021-09-01 東洋紡株式会社 Cell culture method using a hollow fiber module
SG11201809051YA (en) 2016-04-15 2018-11-29 Boehringer Ingelheim Int Cell retention device and method
EP3452575A4 (en) * 2016-05-05 2020-03-11 Terumo BCT, Inc. AUTOMATED MANUFACTURING AND COLLECTION
CN109415696A (en) 2016-05-25 2019-03-01 泰尔茂比司特公司 Cell amplification
US11104874B2 (en) 2016-06-07 2021-08-31 Terumo Bct, Inc. Coating a bioreactor
US11685883B2 (en) 2016-06-07 2023-06-27 Terumo Bct, Inc. Methods and systems for coating a cell growth surface
EP3504315A4 (en) 2016-08-27 2020-04-15 3D Biotek, LLC Bioreactor
US11624046B2 (en) 2017-03-31 2023-04-11 Terumo Bct, Inc. Cell expansion
CN110612344B (en) * 2017-03-31 2023-09-12 泰尔茂比司特公司 cell expansion
CN111065728B (en) * 2017-08-24 2023-12-12 泰尔茂比司特公司 cell expansion
CA3100776A1 (en) * 2018-05-18 2019-11-21 Children's National Medical Center System of cell expansion and methods of using the same
JP6628448B2 (en) * 2018-05-29 2020-01-08 藤森工業株式会社 Shaking culture apparatus and culture method using the same
JP7315653B2 (en) 2018-08-16 2023-07-26 テルモ株式会社 Cell culture substrate
EP3830237A1 (en) 2018-08-16 2021-06-09 TERUMO Kabushiki Kaisha Cell culture substrate
JP2021533736A (en) 2018-08-16 2021-12-09 テルモ株式会社 Cell culture substrate
EP3830238A1 (en) 2018-08-16 2021-06-09 TERUMO Kabushiki Kaisha Cell culture substrate
EP3887497A1 (en) 2018-12-20 2021-10-06 TERUMO Kabushiki Kaisha Cell culture substrate
US12404485B2 (en) 2018-12-20 2025-09-02 Terumo Kabushiki Kaisha Cell culture substrate
EP4025899B1 (en) 2019-09-20 2023-11-08 TERUMO Kabushiki Kaisha Method for evaluating a coating state of either an adsorbent of a sample protein or an adsorption state of a sample protein
CN115735006A (en) * 2020-06-10 2023-03-03 武田药品工业株式会社 Semi-automated hollow fiber system for viral transduction
JP2023072702A (en) 2020-06-18 2023-05-25 テルモ株式会社 Cell culture substrate
EP4396324A1 (en) * 2021-09-30 2024-07-10 Terumo Kabushiki Kaisha Cell culturing device and cell culturing system

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1995004813A1 (en) * 1993-08-06 1995-02-16 Unisyn Technologies, Inc. Hollow fiber bioreactor system with improved nutrient oxygenation
US5622857A (en) * 1995-08-08 1997-04-22 Genespan Corporation High performance cell culture bioreactor and method
WO1998053046A1 (en) * 1997-05-22 1998-11-26 Excorp Medical, Inc. Bioreactor
WO2003105663A2 (en) * 2002-06-01 2003-12-24 Stelsys Llc Liver assist system based on hollow fiber cartridges or rotating bioreactor

Family Cites Families (84)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3821087A (en) 1972-05-18 1974-06-28 Dedrick R Cell culture on semi-permeable tubular membranes
US3896061A (en) 1972-08-16 1975-07-22 Toray Industries Semi-permeable membranes, their preparation and their use
JPS5642584A (en) 1979-09-18 1981-04-20 Asahi Chem Ind Co Ltd Cell cultivation method
JPS5714640A (en) 1980-07-02 1982-01-25 Toray Ind Inc Separating membrane of methyl methacrylate type
US4804628A (en) 1984-10-09 1989-02-14 Endotronics, Inc. Hollow fiber cell culture device and method of operation
WO1986002379A1 (en) 1984-10-09 1986-04-24 Endotronics, Inc. Hollow fiber culture device for improved nutrient perfusion and product concentration and method of operation
US4650766A (en) 1984-10-09 1987-03-17 Endotronics, Inc. Culturing apparatus
US4647539A (en) 1985-05-24 1987-03-03 Endotronics, Inc. Method and apparatus for growing cells in vitro
EP0220650A3 (en) 1985-10-21 1988-08-24 Endotronics Inc. Method and device for culturing cells
US4722902A (en) 1985-11-04 1988-02-02 Endotronics, Inc. Apparatus and method for culturing cells, removing waste and concentrating product
EP0302894B1 (en) 1986-04-28 1992-11-19 Endotronics Inc. Method of culturing leukocytes
US4889812A (en) 1986-05-12 1989-12-26 C. D. Medical, Inc. Bioreactor apparatus
US4894342A (en) 1986-05-12 1990-01-16 C. D. Medical, Inc. Bioreactor system
US4918019A (en) 1986-05-12 1990-04-17 C. D. Medical, Incorporated Bioreactor system with plasticizer removal
WO1988001643A1 (en) 1986-08-29 1988-03-10 Endotronics, Inc. Method of culturing cells
US4885087A (en) * 1986-11-26 1989-12-05 Kopf Henry B Apparatus for mass transfer involving biological/pharmaceutical media
US4973558A (en) 1988-04-28 1990-11-27 Endotronics, Inc. Method of culturing cells using highly gas saturated media
US5605835A (en) 1988-05-23 1997-02-25 Regents Of The University Of Minnesota Bioreactor device with application as a bioartificial liver
US5416022A (en) 1988-08-10 1995-05-16 Cellex Biosciences, Inc. Cell culture apparatus
US5079168A (en) 1988-08-10 1992-01-07 Endotronics, Inc. Cell culture apparatus
WO1990002171A1 (en) 1988-08-31 1990-03-08 Cellco Advanced Bioreactors, Inc. In vitro cell culture reactor
US5162225A (en) 1989-03-17 1992-11-10 The Dow Chemical Company Growth of cells in hollow fibers in an agitated vessel
JPH066053B2 (en) * 1989-03-17 1994-01-26 タバイエスペック株式会社 Cell culture device
US5763266A (en) 1989-06-15 1998-06-09 The Regents Of The University Of Michigan Methods, compositions and devices for maintaining and growing human stem and/or hematopoietics cells
US5399493A (en) 1989-06-15 1995-03-21 The Regents Of The University Of Michigan Methods and compositions for the optimization of human hematopoietic progenitor cell cultures
US5437994A (en) 1989-06-15 1995-08-01 Regents Of The University Of Michigan Method for the ex vivo replication of stem cells, for the optimization of hematopoietic progenitor cell cultures, and for increasing the metabolism, GM-CSF secretion and/or IL-6 secretion of human stromal cells
US5605822A (en) 1989-06-15 1997-02-25 The Regents Of The University Of Michigan Methods, compositions and devices for growing human hematopoietic cells
US5635386A (en) 1989-06-15 1997-06-03 The Regents Of The University Of Michigan Methods for regulating the specific lineages of cells produced in a human hematopoietic cell culture
US5510257A (en) 1989-10-04 1996-04-23 Sirkar; Kamalesh K. Hollow fiber immobilization with chopped microporous hollow fibers
AU7787191A (en) 1989-11-09 1991-06-13 Bio-Metric Systems, Inc. Improved bioreactor surfaces and methods of making same
US5126238A (en) 1990-02-15 1992-06-30 Unisyn Fibertec Corporation Hollow fiber cell propagation system and method
US5656421A (en) 1990-02-15 1997-08-12 Unisyn Technologies, Inc. Multi-bioreactor hollow fiber cell propagation system and method
US5635387A (en) 1990-04-23 1997-06-03 Cellpro, Inc. Methods and device for culturing human hematopoietic cells and their precursors
US6326198B1 (en) 1990-06-14 2001-12-04 Regents Of The University Of Michigan Methods and compositions for the ex vivo replication of stem cells, for the optimization of hematopoietic progenitor cell cultures, and for increasing the metabolism, GM-CSF secretion and/or IL-6 secretion of human stromal cells
US5202254A (en) 1990-10-11 1993-04-13 Endotronics, Inc. Process for improving mass transfer in a membrane bioreactor and providing a more homogeneous culture environment
CA2098510A1 (en) 1990-12-13 1992-06-14 Kenneth W. Culver Sustained and continuous production of high titers of recombinant viral vectors and transduced target cells for use in gene therapy
US5330915A (en) 1991-10-18 1994-07-19 Endotronics, Inc. Pressure control system for a bioreactor
US5763194A (en) 1993-10-29 1998-06-09 Unisearch Limited Cell separation device
JPH09511392A (en) 1994-02-09 1997-11-18 ユニシン・テクノロジーズ・インコーポレーテッド High performance cell culture bioreactor and method
US5882918A (en) 1995-08-08 1999-03-16 Genespan Corporation Cell culture incubator
US6228635B1 (en) 1995-06-07 2001-05-08 Aastrom Bioscience, Inc. Portable cell growth cassette for use in maintaining and growing biological cells
US6238908B1 (en) 1995-06-07 2001-05-29 Aastrom Biosciences, Inc. Apparatus and method for maintaining and growth biological cells
US6096532A (en) 1995-06-07 2000-08-01 Aastrom Biosciences, Inc. Processor apparatus for use in a system for maintaining and growing biological cells
US5985653A (en) 1995-06-07 1999-11-16 Aastrom Biosciences, Inc. Incubator apparatus for use in a system for maintaining and growing biological cells
US5688687A (en) 1995-06-07 1997-11-18 Aastrom Biosciences, Inc. Bioreactor for mammalian cell growth and maintenance
US5627070A (en) 1995-07-26 1997-05-06 Celltherapy, Inc. Cell growing device for in vitro cell population expansion
WO1997016527A1 (en) 1995-10-30 1997-05-09 Cellex Biosciences, Inc. Cultureware for bioartificial liver
US5998184A (en) 1997-10-08 1999-12-07 Unisyn Technologies, Inc. Basket-type bioreactor
US6001585A (en) 1997-11-14 1999-12-14 Cellex Biosciences, Inc. Micro hollow fiber bioreactor
US20020034819A1 (en) 1998-02-23 2002-03-21 Alan K. Smith Human lineage committed cell composition with enhanced proliferative potential, biological effector function, or both; methods for obtaining same; and their uses
US6096523A (en) * 1998-11-04 2000-08-01 University Of Georgia Research Foundation Transformation vector system
US6979308B1 (en) 1999-06-03 2005-12-27 University Of North Carolina At Chapel Hill Bioreactor design and process for engineering tissue from cells
AU780160B2 (en) 1999-06-03 2005-03-03 University Of North Carolina At Chapel Hill, The Bioreactor design and process for engineering tissue from cells
DE19932439C2 (en) 1999-07-12 2002-06-13 Sefar Ag Rueschlikon bioreactor
AUPQ319199A0 (en) 1999-09-30 1999-10-28 Unisearch Limited Method and apparatus for culturing cells
AU2001282908A1 (en) 2000-08-14 2002-02-25 University Of Maryland Biotechnology Institute Bioreactor and bioprocessing technique
US20070122904A1 (en) 2000-09-29 2007-05-31 Unisearch Limited Method and apparatus for culturing cells
EP1325110A4 (en) * 2000-10-02 2009-03-25 Thomas F Cannon Automated bioculture and bioculture experiments system
US6642019B1 (en) 2000-11-22 2003-11-04 Synthecan, Inc. Vessel, preferably spherical or oblate spherical for growing or culturing cells, cellular aggregates, tissues and organoids and methods for using same
US6616912B2 (en) 2001-01-05 2003-09-09 Spectrum Laboratories, Inc. Bi-component microporous hollow fiber membrane structure for in vivo propagation of cells
US6582955B2 (en) 2001-05-11 2003-06-24 Spectrum Laboratories, Inc. Bioreactor with application as blood therapy device
US6566126B2 (en) 2001-06-22 2003-05-20 Fibercell Systems, Inc. Apparatus and method for growing cells
JP2003070464A (en) 2001-09-04 2003-03-11 Mitsubishi Heavy Ind Ltd Method for culturing cell, method for producing artificial organ and the resultant artificial organ
US7154974B2 (en) * 2002-02-12 2006-12-26 Broadcom Corporation Data recovery system and applications thereof in radio receivers
US7033823B2 (en) 2002-01-31 2006-04-25 Cesco Bioengineering, Inc. Cell-cultivating device
JP2003332274A (en) 2002-05-17 2003-11-21 Tokyo Seimitsu Co Ltd Chemical mechanical polishing method and chemical mechanical polishing apparatus
US6943008B1 (en) 2002-08-21 2005-09-13 Florida State University Research Foundation, Inc. Bioreactor for cell culture
CA2784829C (en) 2003-04-01 2015-10-06 United States Of America Department Of Veteran's Affairs Stem-cell, precursor cell, or target cell-based treatment of multi-organ failure and renal dysfunction
US7432101B2 (en) * 2003-07-22 2008-10-07 Vivalis Production of poxviruses with adherent or non adherent avian cell lines
DE10336152B4 (en) 2003-08-06 2007-02-15 Nephrogen LLC, Salt Lake City Purification method for human mesenchymal stem cells
US7172696B1 (en) 2004-01-02 2007-02-06 Spectrum Laboratories, Inc. Radial dispersion mass transfer device having a semi-permeable tubular hollow fiber membrane wound around a porous core
WO2005087915A2 (en) 2004-03-08 2005-09-22 Biovest International, Inc. Use of ethanolamine for enhancing cell growth in membrane systems
JP2005278564A (en) * 2004-03-30 2005-10-13 Hitachi Medical Corp Cell culture apparatus
US7531351B2 (en) 2004-06-14 2009-05-12 Probiogen Ag Liquid-gas-phase exposure reactor for cell culturing
JP2007000038A (en) * 2005-06-22 2007-01-11 Toray Ind Inc Closed-system circulatory circuit-type culturing device
US7682822B2 (en) 2006-03-31 2010-03-23 Aastrom Biosciences, Inc. Ex vivo generated tissue system
WO2007139747A1 (en) 2006-05-22 2007-12-06 Biovest International Inc. Interface of a cultureware module in a cell culture system and installation method thereof
JP5550902B2 (en) 2006-06-26 2014-07-16 テルモ ビーシーティー、インコーポレーテッド Method for culturing mesenchymal stem cells
JP5149313B2 (en) 2007-03-01 2013-02-20 テルモ ビーシーティー、インコーポレーテッド Disposable piping set for use with cell growth apparatus and aseptic sampling method
CA2680130C (en) 2007-03-05 2016-01-12 Caridianbct, Inc. Cell expansion system and methods of use
ES2665024T3 (en) 2007-03-05 2018-04-24 Terumo Bct, Inc. Methods to control cell movement in hollow fiber bioreactors
CA2679160A1 (en) 2007-03-14 2008-09-18 Glen Delbert Antwiler Cell expansion apparatus with plate bioreactor
CA2681461A1 (en) 2007-04-06 2008-10-16 Caridianbct, Inc. Improved bioreactor surfaces
WO2008128165A2 (en) 2007-04-13 2008-10-23 Caridianbct, Inc. Cell expansion system and methods of use

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1995004813A1 (en) * 1993-08-06 1995-02-16 Unisyn Technologies, Inc. Hollow fiber bioreactor system with improved nutrient oxygenation
US5622857A (en) * 1995-08-08 1997-04-22 Genespan Corporation High performance cell culture bioreactor and method
WO1998053046A1 (en) * 1997-05-22 1998-11-26 Excorp Medical, Inc. Bioreactor
WO2003105663A2 (en) * 2002-06-01 2003-12-24 Stelsys Llc Liver assist system based on hollow fiber cartridges or rotating bioreactor

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JP2013176377A (en) 2013-09-09

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