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WO2008108843A3 - Procédés et kits pour amplifier l'adn - Google Patents

Procédés et kits pour amplifier l'adn Download PDF

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Publication number
WO2008108843A3
WO2008108843A3 PCT/US2007/063103 US2007063103W WO2008108843A3 WO 2008108843 A3 WO2008108843 A3 WO 2008108843A3 US 2007063103 W US2007063103 W US 2007063103W WO 2008108843 A3 WO2008108843 A3 WO 2008108843A3
Authority
WO
WIPO (PCT)
Prior art keywords
methods
products
disclosed
nucleic acid
appearance
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/US2007/063103
Other languages
English (en)
Other versions
WO2008108843A2 (fr
Inventor
Michael M Becker
Wai-Chung Lam
Kristin W Livezey
Steven T Brentano
Daniel P Kolk
Astrid R W Schroder
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Gen Probe Inc
Original Assignee
Gen Probe Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Gen Probe Inc filed Critical Gen Probe Inc
Priority to CA002678799A priority Critical patent/CA2678799A1/fr
Priority to PCT/US2007/063103 priority patent/WO2008108843A2/fr
Priority to JP2009551981A priority patent/JP2010520750A/ja
Priority to AU2007348215A priority patent/AU2007348215A1/en
Priority to EP07757746A priority patent/EP2118305A4/fr
Publication of WO2008108843A2 publication Critical patent/WO2008108843A2/fr
Publication of WO2008108843A3 publication Critical patent/WO2008108843A3/fr
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6865—Promoter-based amplification, e.g. nucleic acid sequence amplification [NASBA], self-sustained sequence replication [3SR] or transcription-based amplification system [TAS]

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

L'invention concerne de nouveaux procédés de synthèse de plusieurs copies d'une séquence cible d'acide nucléique, qui sont autocatalytiques (à savoir, capables d'effectuer des cycles automatiquement sans nécessité de modifier les conditions de réaction telles que la température, le pH ou la force ionique et en utilisant le produit d'un cycle dans le suivant). En particulier, l'invention concerne des procédés d'amplification d'acide nucléique, qui sont robustes et efficaces, tout en réduisant l'apparition de produits secondaires. De manière générale, les procédés utilisent des oligonucléotides amorces, qui ciblent seulement un sens de l'acide nucléique cible, un oligonucléotide promoteur modifié pour prévenir l'extension par polymérase depuis son extrémité 3' et le cas échéant, un moyen pour terminer une réaction d'extension d'amorce, pour amplifier des molécules d'ARN ou d'ADN in vitro, tout en réduisant ou en éliminant sensiblement la formation de produits secondaires. Les procédés décrits minimisent ou éliminent sensiblement l'émergence de produits secondaires, produisant ainsi un niveau élevé de spécificité. De plus, l'apparition de produits secondaires peut compliquer l'analyse de la réaction d'amplification par différentes techniques de détection moléculaire. Les procédés décrits minimisent ou éliminent sensiblement ce problème, procurant ainsi des niveaux amplifiés de sensibilité.
PCT/US2007/063103 2007-03-01 2007-03-01 Procédés et kits pour amplifier l'adn Ceased WO2008108843A2 (fr)

Priority Applications (5)

Application Number Priority Date Filing Date Title
CA002678799A CA2678799A1 (fr) 2007-03-01 2007-03-01 Procedes et kits pour amplifier l'adn
PCT/US2007/063103 WO2008108843A2 (fr) 2007-03-01 2007-03-01 Procédés et kits pour amplifier l'adn
JP2009551981A JP2010520750A (ja) 2007-03-01 2007-03-01 Ｄｎａを増幅するための方法およびキット
AU2007348215A AU2007348215A1 (en) 2007-03-01 2007-03-01 Methods and kits for amplifying DNA
EP07757746A EP2118305A4 (fr) 2007-03-01 2007-03-01 Procédés et kits pour amplifier l'adn

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
PCT/US2007/063103 WO2008108843A2 (fr) 2007-03-01 2007-03-01 Procédés et kits pour amplifier l'adn

Publications (2)

Publication Number Publication Date
WO2008108843A2 WO2008108843A2 (fr) 2008-09-12
WO2008108843A3 true WO2008108843A3 (fr) 2008-10-30

Family

ID=39738944

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2007/063103 Ceased WO2008108843A2 (fr) 2007-03-01 2007-03-01 Procédés et kits pour amplifier l'adn

Country Status (5)

Country Link
EP (1) EP2118305A4 (fr)
JP (1) JP2010520750A (fr)
AU (1) AU2007348215A1 (fr)
CA (1) CA2678799A1 (fr)
WO (1) WO2008108843A2 (fr)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9074246B2 (en) 2010-01-25 2015-07-07 Rd Biosciences, Inc. Self-folding amplification of target nucleic acid

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1975242B1 (fr) * 2004-08-27 2011-03-02 Gen-Probe Incorporated Procédés d'amplification d'acide nucléique à simple amorce
WO2011003020A1 (fr) * 2009-07-01 2011-01-06 Gen-Probe Incorporated Procédés et compositions pour l'amplification d'acide nucléique
AU2013308647B2 (en) * 2012-08-30 2018-10-18 Gen-Probe Incorporated Multiphase nucleic acid amplification
WO2016018963A1 (fr) 2014-07-30 2016-02-04 President And Fellows Of Harvard College Construction d'une bibliothèque de sondes
US11788123B2 (en) 2017-05-26 2023-10-17 President And Fellows Of Harvard College Systems and methods for high-throughput image-based screening
CN109020974B (zh) * 2018-11-02 2021-01-05 哈尔滨商业大学 5′-噻吩甲酰酯-3′-脱氧腺苷的合成方法及其应用
CN113366116A (zh) 2018-12-13 2021-09-07 哈佛学院院长及董事 用于merfish和其他应用的扩增方法和系统

Citations (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5169766A (en) * 1991-06-14 1992-12-08 Life Technologies, Inc. Amplification of nucleic acid molecules
US5409818A (en) * 1988-02-24 1995-04-25 Cangene Corporation Nucleic acid amplification process
US5554516A (en) * 1992-05-06 1996-09-10 Gen-Probe Incorporated Nucleic acid sequence amplification method, composition and kit
US20030194723A1 (en) * 2001-11-02 2003-10-16 Cunningham Melissa M. Compositions, methods and kits for determining the presence of Mycoplasma pneumoniae in a test sample
US20040175729A1 (en) * 2002-11-08 2004-09-09 Sysmex Corporation Primer for nucleic acid amplification to detect carcinoembryonic antigen and test method using such primer
US20060046265A1 (en) * 2004-08-27 2006-03-02 Gen-Probe Incorporated Single-primer nucleic acid amplification methods
US20070202523A1 (en) * 2004-08-27 2007-08-30 Becker Michael M Methods and kits for amplifying dna
US20070281317A1 (en) * 2006-06-06 2007-12-06 Gen-Probe Incorporated Tagged oligonucleotides and their use in nucleic acid amplification methods

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2708288B1 (fr) * 1993-07-26 1995-09-01 Bio Merieux Procédé d'amplification d'acides nucléiques par transcription utilisant le déplacement, réactifs et nécessaire pour la mise en Óoeuvre de ce procédé.

Patent Citations (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5409818A (en) * 1988-02-24 1995-04-25 Cangene Corporation Nucleic acid amplification process
US5169766A (en) * 1991-06-14 1992-12-08 Life Technologies, Inc. Amplification of nucleic acid molecules
US5554516A (en) * 1992-05-06 1996-09-10 Gen-Probe Incorporated Nucleic acid sequence amplification method, composition and kit
US20030194723A1 (en) * 2001-11-02 2003-10-16 Cunningham Melissa M. Compositions, methods and kits for determining the presence of Mycoplasma pneumoniae in a test sample
US20040175729A1 (en) * 2002-11-08 2004-09-09 Sysmex Corporation Primer for nucleic acid amplification to detect carcinoembryonic antigen and test method using such primer
US20060046265A1 (en) * 2004-08-27 2006-03-02 Gen-Probe Incorporated Single-primer nucleic acid amplification methods
WO2006026388A2 (fr) * 2004-08-27 2006-03-09 Gen-Probe Incorporated Techniques d'amplification d'acide nucleique avec une seule amorce
US20070202523A1 (en) * 2004-08-27 2007-08-30 Becker Michael M Methods and kits for amplifying dna
US20070299254A1 (en) * 2004-08-27 2007-12-27 Gen-Probe Incorporated Kits for Performing Amplification Reactions
US20070281317A1 (en) * 2006-06-06 2007-12-06 Gen-Probe Incorporated Tagged oligonucleotides and their use in nucleic acid amplification methods

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
See also references of EP2118305A4 *

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9074246B2 (en) 2010-01-25 2015-07-07 Rd Biosciences, Inc. Self-folding amplification of target nucleic acid

Also Published As

Publication number Publication date
AU2007348215A1 (en) 2008-09-12
EP2118305A4 (fr) 2010-11-10
CA2678799A1 (fr) 2008-09-12
JP2010520750A (ja) 2010-06-17
WO2008108843A2 (fr) 2008-09-12
EP2118305A2 (fr) 2009-11-18

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