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WO2008149809A1 - Cell chip for evaluation of cellular motility - Google Patents

Cell chip for evaluation of cellular motility Download PDF

Info

Publication number
WO2008149809A1
WO2008149809A1 PCT/JP2008/060032 JP2008060032W WO2008149809A1 WO 2008149809 A1 WO2008149809 A1 WO 2008149809A1 JP 2008060032 W JP2008060032 W JP 2008060032W WO 2008149809 A1 WO2008149809 A1 WO 2008149809A1
Authority
WO
WIPO (PCT)
Prior art keywords
evaluation
substance
chip
cell
interest
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/JP2008/060032
Other languages
French (fr)
Japanese (ja)
Inventor
Satoshi Fujita
Akira Nagasaki
Jun Miyake
Reiko Nagasaki
Masato Miyake
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
National Institute of Advanced Industrial Science and Technology AIST
Original Assignee
National Institute of Advanced Industrial Science and Technology AIST
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by National Institute of Advanced Industrial Science and Technology AIST filed Critical National Institute of Advanced Industrial Science and Technology AIST
Publication of WO2008149809A1 publication Critical patent/WO2008149809A1/en
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

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Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • G01N33/5008—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
    • G01N33/502—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics for testing non-proliferative effects
    • G01N33/5029—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics for testing non-proliferative effects on cell motility

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Immunology (AREA)
  • Hematology (AREA)
  • Cell Biology (AREA)
  • Chemical & Material Sciences (AREA)
  • Urology & Nephrology (AREA)
  • Molecular Biology (AREA)
  • Toxicology (AREA)
  • Physics & Mathematics (AREA)
  • Biotechnology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Microbiology (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

Disclosed is an evaluation system which can observe the change in motility of a cell containing a substance of interest over time. In the evaluation system, a cellular motility evaluation chip is used, in which a spot on a substrate of the chip is fluorescently labeled and a nucleic acid labeled with other fluorescent substance is contained in the spot together with the substance of interest. The evaluation is made by plating a cell of interest on the chip and observing the mobility of the two fluorescent substances, based on a transfection array method. Also disclosed is a method for the screening of a substance capable of inhibiting the movement of a cell.
PCT/JP2008/060032 2007-05-30 2008-05-30 Cell chip for evaluation of cellular motility Ceased WO2008149809A1 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
JP2007-144215 2007-05-30
JP2007144215A JP5024823B2 (en) 2007-05-30 2007-05-30 Cell motility evaluation cell chip

Publications (1)

Publication Number Publication Date
WO2008149809A1 true WO2008149809A1 (en) 2008-12-11

Family

ID=40093627

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/JP2008/060032 Ceased WO2008149809A1 (en) 2007-05-30 2008-05-30 Cell chip for evaluation of cellular motility

Country Status (2)

Country Link
JP (1) JP5024823B2 (en)
WO (1) WO2008149809A1 (en)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN114303061A (en) * 2019-08-15 2022-04-08 尤瓦尔·林克维奇 Modulating extracellular matrix movement

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2002077264A2 (en) * 2001-03-22 2002-10-03 Whitehead Institute For Biomedical Research Arrayed transfection method and uses related thereto
WO2003058193A2 (en) * 2001-07-02 2003-07-17 The Board Of Trustees Of The Leland Stanford Junior University Microarrays for cell phenotyping and manipulation
WO2005001090A1 (en) * 2003-06-26 2005-01-06 National Institute Of Advanced Industrial Science And Technology Method and composition for improving efficiency of transferring nucleic acid into cell
WO2006046217A2 (en) * 2004-10-29 2006-05-04 National Institute Of Advanced Industrial Science And Technology Methods and systems for analyzing a network of biological functions

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1999015629A1 (en) * 1997-09-25 1999-04-01 Cytomatrix, Llc Methods and devices for the long-term culture of hematopoietic progenitor cells
CA2330104A1 (en) * 1998-04-09 1999-10-21 Charlotte-Mecklenberg Hospital Authority Creation of three-dimensional tissues
ATE383417T1 (en) * 1998-11-12 2008-01-15 Cytomatrix Llc PRODUCTION OF LYMPHOID TISSUE-SPECIFIC CELLS FROM HEMATOPOIETIC PRECURSOR CELLS IN A THREE-DIMENSIONAL SYSTEM

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2002077264A2 (en) * 2001-03-22 2002-10-03 Whitehead Institute For Biomedical Research Arrayed transfection method and uses related thereto
WO2003058193A2 (en) * 2001-07-02 2003-07-17 The Board Of Trustees Of The Leland Stanford Junior University Microarrays for cell phenotyping and manipulation
WO2005001090A1 (en) * 2003-06-26 2005-01-06 National Institute Of Advanced Industrial Science And Technology Method and composition for improving efficiency of transferring nucleic acid into cell
WO2006046217A2 (en) * 2004-10-29 2006-05-04 National Institute Of Advanced Industrial Science And Technology Methods and systems for analyzing a network of biological functions

Also Published As

Publication number Publication date
JP2008295351A (en) 2008-12-11
JP5024823B2 (en) 2012-09-12

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