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WO2008039997A3 - Nucleobase conjugates with cationic backbone linkers - Google Patents

Nucleobase conjugates with cationic backbone linkers Download PDF

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Publication number
WO2008039997A3
WO2008039997A3 PCT/US2007/079935 US2007079935W WO2008039997A3 WO 2008039997 A3 WO2008039997 A3 WO 2008039997A3 US 2007079935 W US2007079935 W US 2007079935W WO 2008039997 A3 WO2008039997 A3 WO 2008039997A3
Authority
WO
WIPO (PCT)
Prior art keywords
conjugates
methods
cationic backbone
nucleobase
label
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/US2007/079935
Other languages
French (fr)
Other versions
WO2008039997A2 (en
Inventor
Paul M Kenney
Shaheer H Khan
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Applied Biosystems Inc
Original Assignee
Applera Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Applera Corp filed Critical Applera Corp
Publication of WO2008039997A2 publication Critical patent/WO2008039997A2/en
Publication of WO2008039997A3 publication Critical patent/WO2008039997A3/en
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H19/00—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof
    • C07H19/02—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof sharing nitrogen
    • C07H19/04—Heterocyclic radicals containing only nitrogen atoms as ring hetero atom
    • C07H19/06—Pyrimidine radicals
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H19/00—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof
    • C07H19/02—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof sharing nitrogen
    • C07H19/04—Heterocyclic radicals containing only nitrogen atoms as ring hetero atom
    • C07H19/06—Pyrimidine radicals
    • C07H19/10—Pyrimidine radicals with the saccharide radical esterified by phosphoric or polyphosphoric acids
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H19/00—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof
    • C07H19/02—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof sharing nitrogen
    • C07H19/04—Heterocyclic radicals containing only nitrogen atoms as ring hetero atom
    • C07H19/16—Purine radicals
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H19/00—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof
    • C07H19/02—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof sharing nitrogen
    • C07H19/04—Heterocyclic radicals containing only nitrogen atoms as ring hetero atom
    • C07H19/16—Purine radicals
    • C07H19/20—Purine radicals with the saccharide radical esterified by phosphoric or polyphosphoric acids
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

Provided are nucleotide-label conjugates and related compounds in which a label, such as a fluorescent dye, is linked to a nucleobase directly or indirectly by a linker. In some embodiments, the linker comprises a backbone comprising at least one imidazolium moiety. Such conjugates are useful, for example, in primer extension methods and related techniques, and in methods that involve electrophoresis of polynucleotides, such as certain DNA sequencing techniques. Methods, kits, and compositions are also disclosed.
PCT/US2007/079935 2006-09-28 2007-09-28 Nucleobase conjugates with cationic backbone linkers Ceased WO2008039997A2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US84831606P 2006-09-28 2006-09-28
US60/848,316 2006-09-28

Publications (2)

Publication Number Publication Date
WO2008039997A2 WO2008039997A2 (en) 2008-04-03
WO2008039997A3 true WO2008039997A3 (en) 2008-05-22

Family

ID=39204596

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2007/079935 Ceased WO2008039997A2 (en) 2006-09-28 2007-09-28 Nucleobase conjugates with cationic backbone linkers

Country Status (1)

Country Link
WO (1) WO2008039997A2 (en)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
USRE46683E1 (en) 2008-03-31 2018-01-23 Applied Biosystems, Llc Nucleic acid terminators incorporating a cationic moiety and methods for their use

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2001014345A1 (en) * 1999-08-20 2001-03-01 The Research Foundation Of State University Of New York 5-propynylpyrimidine derivatives as cytotoxic enzyme inhibitors
WO2001049790A2 (en) * 2000-01-04 2001-07-12 Applera Corporation Mobility-modifying cyanine dyes
WO2002030944A2 (en) * 2000-10-11 2002-04-18 Applera Corporation Fluorescent nucleobase conjugates having anionic linkers

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2001014345A1 (en) * 1999-08-20 2001-03-01 The Research Foundation Of State University Of New York 5-propynylpyrimidine derivatives as cytotoxic enzyme inhibitors
WO2001049790A2 (en) * 2000-01-04 2001-07-12 Applera Corporation Mobility-modifying cyanine dyes
WO2002030944A2 (en) * 2000-10-11 2002-04-18 Applera Corporation Fluorescent nucleobase conjugates having anionic linkers

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
USRE46683E1 (en) 2008-03-31 2018-01-23 Applied Biosystems, Llc Nucleic acid terminators incorporating a cationic moiety and methods for their use

Also Published As

Publication number Publication date
WO2008039997A2 (en) 2008-04-03

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