WO2008038744A1 - Bactericidal composition - Google Patents

Abstract

A bactericidal composition comprising (A) an active oxygen compound, (B) an inorganic acid, (C) and a nitrate salt (provided that silver nitrate is excluded).

Description

Disinfectant composition

Technical field

 The present invention relates to a disinfectant composition and a disinfecting method using the same.

Conventional technology ''

 Currently, there are various known chemicals used for sterilization and disinfection purposes. Especially, sodium hypochlorite is an oxygen-based disinfectant such as hydrogen peroxide and hydrogen peroxide in water. Percarbonates, perborates, etc., that generate water are mainly used. However, these fungicides have various problems. For example, sodium hypochlorite is corrosive to metals, has a handling problem of generating chlorine gas when mixed with acidic substances, and also generates carcinogenic trihalomethanes in the environment. have. Hydrogen peroxide needs to act for a long time at a high concentration in order to obtain a high degree of bactericidal effect. A high bactericidal ability is obtained by using an organic acid in combination with hydrogen peroxide as a bactericidal component. Acetic acid is selected as the organic acid to be used in terms of stability and bactericidal effect, but there are still problems when the irritating odor and the BOD (biochemical oxygen demand) of the wastewater are high.

 JP-A6-71269 discloses a sterilization method that can disinfect general bacteria in a relatively short time of about 30 minutes by heating a hydrogen peroxide solution of 0.1% or less to 30-60 ° C. It has been done. JP-A 11-76380 discloses a disinfectant cleaning agent for medical equipment comprising hydrogen peroxide, an organic acid, an organic peracid and a stabilizer. US4557898 discloses a composition for sterilizing spores by adding various additives to hydrogen peroxide.

 JP-A8-225418 discloses a disinfectant comprising silver, an inorganic acid, an organic stabilizer and hydrogen peroxide.

Disclosure of the invention

 The present invention comprises (A) an active oxygen compound (hereinafter referred to as component (A)), (B) an inorganic acid (hereinafter referred to as component (B)), and (C) a nitrate (hereinafter referred to as component (C)). However, it relates to a disinfectant composition that does not contain silver nitrate.

 The present invention provides a fungicide composition obtained by blending (A) an active oxygen compound, (B) an inorganic acid, and (C) a nitrate, but not containing silver nitrate.

Furthermore, the present invention provides a bactericidal product comprising the bactericidal composition as one agent, a bactericidal method comprising bringing the bactericidal composition or the bactericidal product into contact with an object, or the composition Or provide the use of the above product as a disinfectant.

Detailed Description of the Invention

 JP—A6—71269 is not effective enough when higher bactericidal power is required, such as sterilization of spores. J P-Al-76380 contains organic peracids, so it does not solve the problems of irritating odor and BOD in waste water. In US4557898, it has been shown that heating to about 50 ° C can increase the spore kill rate, but the problem is that the hydrogen peroxide concentration is quite high. In addition to the low economics of high hydrogen peroxide concentration, there is a cost for treating wastewater after sterilization.

 JP P-A8-225418 Disinfectant contains silver nitrate and has poor stability.

The present invention provides an excellent bactericidal effect against spore bacteria, and can provide a bactericidal composition having a low BOD, for example, capable of killing spore bacteria at a concentration of 10 ⁶ cfuZmL or more. It is providing the sterilization method using such a disinfectant composition.

According to the present invention, a bactericidal composition having a high bactericidal effect against spore bacteria and capable of killing spore bacteria having a concentration of, for example, 10 ⁶ cfuZmL or more can be obtained. In addition, the fungicidal composition of the present invention contains a relatively low concentration of hydrogen peroxide, and further has a reduced BOD component. The present invention has no irritating odor, low BOD, and hydrogen peroxide. A fungicide composition having a reduced concentration of active oxygen compound such as is provided. It also has excellent storage stability.

 According to the sterilization method using the bactericide composition of the present invention, the microorganisms can be sterilized from various objects other than foods and human bodies in which various microorganisms exist. For example, for bacteria, enterococcus, Salmonella, Staphylococcus aureus, Pseudomonas aeruginosa, and fungi, black koujikapi, candida, and the like. Furthermore, bacterial spores such as Bacillus subtilis that exhibit strong resistance to fungicides and heat resistance, and fungal spores such as Aspergillus niger and Candida are targeted. Bacterial spores are dormant cells produced in an environment that is not suitable for growth, and have multiple layered outer shells outside the cells, which are highly resistant to drugs and heat. However, the fungicidal composition of the present invention provides a high bactericidal effect against these spores.

 <(A) component>

 Examples of the active oxygen compound of the present invention include hydrogen peroxide or a compound that generates hydrogen peroxide in water, and hydrogen peroxide is preferable. As compounds that generate hydrogen peroxide in water, solid compounds such as percarbonate, perborate, in particular sodium percarbonate, sodium perborate, and powdery solids and aqueous hydrogen peroxide solutions are used. it can. Component (A) is effective for sterilization and protein removal.

 <(B) Component>

Examples of inorganic acids include hydrochloric acid, sulfuric acid, nitric acid and phosphoric acid. Among them, hydrochloric acid, sulfur The acid, nitric acid and phosphoric acid can be used alone or in combination. Component (B) is effective for sterilization and scale removal.

 <(C) Component>

 Examples of nitrates include alkali metal salts of nitric acid such as sodium nitrate and potassium nitrate, and alkaline earth metal salts of nitric acid such as magnesium nitrate and calcium nitrate, and these can be used alone or in combination. Of these, sodium nitrate, potassium nitrate and magnesium nitrate are preferred. Component (C) is effective for sterilization and fungicidal action. In particular, by coexisting the (A) component and the (B) component with the (C) component, a synergistic effect is obtained in the bactericidal action, and the total of the (A) component, (B) component, and (C) component The amount used can be reduced. However, silver nitrate is not used in the present invention.

 <Fungicide composition>

 The fungicide composition of the present invention preferably contains 2 to 20% by weight, more preferably 4 to 10% by weight, particularly 4 to 8% by weight of the component (A) as a high-concentration fungicide composition. The component (B) is preferably contained in an amount of 2 to 20% by weight, more preferably 4 to 15% by weight, and particularly preferably 6 to 12% by weight. The component (C) is preferably contained in an amount of 1 to 20% by weight, more preferably 1 to 10% by weight, and particularly preferably 1 to 5% by weight. Within these ranges, it is easy to handle and excellent in stability.

 In use, the disinfectant composition of the present invention is obtained by appropriately diluting the disinfectant composition having a high concentration as described above with water, and further heating the disinfectant composition to 35 ° C or more and 70 ° C or less to contact the object. Can be sterilized and washed. Usually, the high-concentration fungicide composition is used by diluting 10 to 150 times.

 At the time of use, the diluting solution of the fungicide composition of the present invention comprises (A) component in an amount of 0.05 to 2.0% by weight, further 0.1 to 1.0% by weight, particularly 0.2 to 0.5%. It is preferable to contain it by weight. Further, the component (B) is preferably contained in an amount of 0.05 to 1.0% by weight, more preferably 0.1 to 0.8% by weight, particularly preferably 0.2 to 0.5% by weight. The component (C) is preferably contained in an amount of 0.02 to 2.0% by weight, more preferably 0.05 to 1.0% by weight, and particularly preferably 0.1 to 0.5% by weight. Within these ranges, a better sterilizing effect can be obtained, the load of wastewater treatment can be reduced, and the economic efficiency can be improved. In addition, when the component (A) and the component (B) are within these ranges, the influence on the apparatus members is small. A composition containing the components (A) to (C) at this concentration can be directly used for sterilization and washing.

 The disinfectant composition of the present invention can be used for food processing equipment and medical equipment. Medical devices include: artificial dialysis machines (including peripheral devices), endoscopes and other endoscopic instruments such as biopsy forceps, scissors, tweezers, forceps, needle holders, colposcope , Retractors, serpentine tubes, tubes, catheters, carts, etc. In particular, the fungicide composition of the present invention is suitable for use in an artificial permeation apparatus.

The disinfectant composition of the present invention is preferably used for disinfecting and cleaning an artificial hemodialysis machine. In the case of an artificial hemodialysis machine, in addition to sterilization, it is required to remove calcium carbonate derived from the dialysate composition and to wash away protein stains removed from the blood by dialysis. In the case of endoscopes, sterilization and removal of protein stains are required, and the fungicide composition of the present invention It becomes the target of adaptation of things.

 The calcium carbonate can be removed by the action of the inorganic acid contained in the fungicide composition of the present invention. Regarding the removal of protein, the active oxygen compound contained therein and further an organic acid [hereinafter referred to as component (D) It can be removed by the action of

 As the component (D) of the present invention, citrate, malic acid, tartaric acid, lactic acid, darconic acid, glycolic acid, thioglycolic acid, malonic acid, succinic acid, glutaric acid, maleic acid, crotonic acid, pyruvic acid And mandelic acid. In the present invention, the content of the component (D) is preferably 0.1 to 2% by weight, more preferably 0.2 to 2% by weight, and particularly preferably 0.4 to 1% by weight in a high-concentration fungicide composition. . Within these ranges, it is easy to handle and excellent in stability. In addition, the content of the component (D) is 0.02 to 0.2% by weight, more preferably 0.03 to 0.15% by weight, particularly 0.05 to 0.1% by weight in the diluted solution at the time of use. It is preferable because it has excellent protein removability and has little influence on the stability of component (A), especially hydrogen peroxide.

 In the bactericidal composition of the present invention, from the viewpoint of bactericidal performance, the weight ratio of the component (A) to the component (B) is (B) Z (A) 0.1-5, further 0.5-3, In particular, it is preferably 1 to 2,

The weight ratio of the component (A) to the component (C) is preferably 0.1 to 5, more preferably 0.2 to 2, and particularly preferably 0.5 to 1 in terms of (C) Z (A).

 The disinfectant composition of the present invention can be a liquid composition containing the above components (A) to (C) and other components such as the component (D) and water. As components other than the component (D), surfactants that do not affect the stability of hydrogen peroxide (especially nonionic surfactants), antifungal agents, hydrogen peroxide stabilizers, etc. It is done. Antifungal agents include aminotril (methylenephosphonic acid) and its salts, 1-hydroxyethylidene-1,1-diphosphonic acid and its salts, ethylenediaminetetra (methylenephosphonic acid) and its salts, and ethylene triamine pens. (Methylene phosphonic acid) and salts thereof.

 The disinfectant composition of the present invention is subjected to a disinfecting method for bringing the composition into contact with an object at a temperature of 35 ° C. or higher and 70 ° C. or lower. According to the present invention, (A) component 0.05 to 2.0% by weight,

(B) Component 0.05-: L. 0% by weight, (C) Component 0.02-2.0% by weight, and water-containing disinfectant composition at a temperature of 35 ° C to 70 ° C In this way, a sterilization method for contacting an object is provided. The contact of the disinfectant composition of the present invention with the object is carried out by bringing an appropriate diluent into contact. However, it is important that the diluent and the object are sufficiently brought into contact with each other. In the case of line washing, the method of adjusting the flow rate at the time of passing and the method of adjusting the residence time after passing can be used. When the object to be cleaned is a closed system including a liquid feed line, a method of circulating cleaning at an appropriate flow rate (for example, 0.1 to 50 liters) is preferable. Air bubbling and ultrasonic waves can be used in combination. Example

The following examples describe the practice of the present invention. The examples are illustrative of the invention and are not intended to limit the invention. (1) Bactericidal effect of bacterial spores

Bacillus subtilis ATC C 6051 is pre-cultured on SCD agar medium (manufactured by Nippon Pharmaceutical Co., Ltd.) for 4 weeks at 30 ° C, and then the appropriate amount of the colony formed on the agar medium is scraped off. Suspension in 1 mL of sterile water and microscopic examination confirmed the formation of bacterial spores (hereinafter referred to as spores). This suspension was subjected to centrifugal washing twice, and then adjusted to a bacterial concentration of 10 ⁸ to 10 ⁹ cfu no mL with an appropriate amount of sterile water.

 0.111111 ^ of this spore suspension was inoculated into 1.9 mL of the sterilizing solution in Table 1 and allowed to act under the sterilizing conditions in Table 1. Immediately thereafter, 0.1 mL of this sterilizing solution was added to 1 mL of SCDLP medium (manufactured by Nippon Pharmaceutical Co., Ltd.) containing 1% by weight of sodium thiosulfate to inactivate the sterilizing solution. Spread 0.2 mL of this inactivated solution on a standard agar medium with a diameter of 9 cm, incubate at 35 ° C for 24 hours, and count the number of colonies formed on the medium to determine the number of viable bacteria. It was measured. The results are shown in Table 1. The balance of the sterilizing solution in Table 1 is water.

table 1

(2) Fungicidal effect of mold spores

Black Aspergillus niger (As pergillusniger I F06341) was pre-cultured on potato dextrose agar (manufactured by Nippon Pharmaceutical Co., Ltd.) at 25 ° C for 4 weeks. The bacterial cells generated on the medium were scraped and suspended uniformly in about 5 mL of sterile water using a glass homogenizer. This suspension was washed twice by centrifugation and then adjusted to a bacterial concentration of 10 ⁷ to 10 ⁸ cf mL with an appropriate amount of sterilized water.

 0.1 mL of this spore suspension was inoculated into 1.9 mL of the sterilizing solution shown in Table 2, and allowed to act under the sterilizing conditions shown in Table 2. Immediately thereafter, 0.1 mL of this sterilizing solution was added to 1 mL of SCDLP medium (manufactured by Nippon Pharmaceutical Co., Ltd.) containing 1% sodium thiosulfate to inactivate the sterilizing solution. Spread 0.2 mL of this inactivation solution on a 9 cm diameter potato dextro agar medium, incubate at 25 ° C for 72 hours, and count the number of colonies formed on the medium. The number of remaining viable bacteria was measured. The results are shown in Table 2. The balance of the bactericidal solution in Table 2 is water.

Table 2

(3) Calcium carbonate and protein stain removal

 A part of the line was replaced with a silicon tube contaminated under the following conditions, the line was assembled in a loop shape so that it could be circulated via a pump, and the sterilizing solution in Table 3 was circulated at the specified temperature. After circulation for a predetermined time, the contaminated silicon tube was removed and the internal contamination degree was evaluated. The circulation conditions of the sterilizing solution were as follows: a circulation time of 40 minutes, a circulation temperature of 40 ° C, and a circulation flow rate of 500 mL. The results are shown in Table 3. The balance of the sterilizing solution in Table 3 is water.

* Silicon tube contamination conditions

 A standard dialysis solution (Fuso Yakuhin Kogyo Co., Ltd., Kindly Solution AF-1) was filled in a silicon tube with an inner diameter of 6 mm, and left standing for 3 days. After confirming the precipitation of calcium carbonate, the dialysate was discarded. Further, about 2 mL of horse blood was added to the inner surface of the same silicone tube, and the horse blood was heat-degraded and fixed by placing it horizontally at 60 ° C for 2 hours.

* Evaluation method for protein stains

 After washing, the silicone tube was removed, and approximately 5 cm including the blood stain was cut vertically, and the protein adhesion state was visually evaluated by amide black staining.

 * Calculation method of calcium carbonate adhesion

Fill the remaining 30 cm of the silicone tube used for protein stains with 0.1 N hydrochloric acid solution, elute the adhering calcium carbonate, and measure the Ca concentration of the eluate. The amount of attached calcium carbonate was determined. The value was expressed in units of C a C0 ₃ mgZm in terms of silicon tube lm.

(4) Storage stability

 The stock solutions of Table 4 were stored at 50 for 14 days and the active oxygen concentration was measured. The results are shown in Table 4.

Table 4

Claims

The scope of the claims 1. A disinfectant composition containing (A) an active oxygen compound, (B) an inorganic acid, and (C) nitrate, but not silver nitrate.  2. (A) 0.05 to 2.0% by weight of active oxygen compound, (B) 0.05 to 1.0% by weight of inorganic acid and (C) 0.02 to 2.0% by weight of nitrate The disinfectant composition according to claim 1.  3. The fungicide composition according to claim 1 or 2, further comprising (D) an organic acid in an amount of 0.02 to 2% by weight.  4. The disinfectant composition according to any one of claims 1 to 3, wherein the active oxygen compound is hydrogen peroxide. 5. The disinfectant composition according to any one of claims 1 to 4, wherein the inorganic acid is at least one selected from the group consisting of hydrochloric acid, sulfuric acid, nitric acid and phosphoric acid.  6. The disinfectant composition according to any one of claims 1 to 5, wherein the nitrate is one or more selected from the group consisting of an alkaline metal salt of nitric acid and an alkaline metal salt.  7. The disinfectant composition according to any one of claims 1 to 6, which is used for disinfecting and washing an artificial hemodialysis machine.  8. A fungicidal composition obtained by blending (A) an active oxygen compound, (B) an inorganic acid, and (C) a nitrate, but not containing silver nitrate.  9. A disinfectant product comprising the disinfectant composition according to any one of claims 1 to 8 as one agent.  10. A sterilization method comprising bringing the bactericide composition according to any one of claims 1 to 8 or the bactericide product according to claim 9 into contact with an object.  11. Disinfection comprising bringing the disinfectant composition according to any one of claims 1 to 8 or the disinfectant product according to claim 9 into contact with an object at a temperature of 35 ° C to 70 ° C. Method. 12. Use of the composition according to any one of claims 1 to 8 or the fungicide of the product according to claim 9.  13. The disinfectant cleaning use of the artificial hemodialysis apparatus of the disinfectant composition according to any one of claims 1 to 8 or the disinfectant product according to claim 9.