[go: up one dir, main page]

WO2008032058A3 - Method for sequencing a polynucleotide - Google Patents

Method for sequencing a polynucleotide Download PDF

Info

Publication number
WO2008032058A3
WO2008032058A3 PCT/GB2007/003451 GB2007003451W WO2008032058A3 WO 2008032058 A3 WO2008032058 A3 WO 2008032058A3 GB 2007003451 W GB2007003451 W GB 2007003451W WO 2008032058 A3 WO2008032058 A3 WO 2008032058A3
Authority
WO
WIPO (PCT)
Prior art keywords
polynucleotide
concatemer
sequencing
target polynucleotide
target
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/GB2007/003451
Other languages
French (fr)
Other versions
WO2008032058A2 (en
Inventor
Preben Lexow
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Lingvitae AS
Original Assignee
Lingvitae AS
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Lingvitae AS filed Critical Lingvitae AS
Publication of WO2008032058A2 publication Critical patent/WO2008032058A2/en
Publication of WO2008032058A3 publication Critical patent/WO2008032058A3/en
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Immunology (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

The present invention is a method for sequencing a target polynucleotide, comprising the steps of: (i) ligating the target polynucleotide to a first polynucleotide; (ii) forming a concatemer comprising multiple copies of the product of step (i); (iii) attaching the concatemer to a second polynucleotide such that the second polynucleotide hybridises to portions of the concatemer, but not to regions on the concatemer corresponding to at least a portion of the target polynucleotide; (iv) interrogating one or more bases in those regions not hybridised to the second polynucleotide, to thereby identify the target polynucleotide sequence.
PCT/GB2007/003451 2006-09-13 2007-09-13 Method for sequencing a polynucleotide Ceased WO2008032058A2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
GB0618046A GB0618046D0 (en) 2006-09-13 2006-09-13 Method
GB0618046.7 2006-09-13

Publications (2)

Publication Number Publication Date
WO2008032058A2 WO2008032058A2 (en) 2008-03-20
WO2008032058A3 true WO2008032058A3 (en) 2008-10-09

Family

ID=37309875

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/GB2007/003451 Ceased WO2008032058A2 (en) 2006-09-13 2007-09-13 Method for sequencing a polynucleotide

Country Status (2)

Country Link
GB (1) GB0618046D0 (en)
WO (1) WO2008032058A2 (en)

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN106715710B (en) 2013-03-15 2022-10-25 赛拉诺斯知识产权有限责任公司 Nucleic acid amplification
CA2906824C (en) 2013-03-15 2023-10-03 Theranos, Inc. Nucleic acid amplification
US10450595B2 (en) 2013-03-15 2019-10-22 Theranos Ip Company, Llc Nucleic acid amplification
WO2015076919A1 (en) * 2013-11-22 2015-05-28 Theranos, Inc. Nucleic Acid Amplification
EP3564370B1 (en) 2013-03-15 2022-01-12 Labrador Diagnostics LLC Nucleic acid amplification
SG11201601611WA (en) 2013-09-06 2016-04-28 Theranos Inc Systems and methods for detecting infectious diseases

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2001020039A2 (en) * 1999-09-16 2001-03-22 Curagen Corporation Method of sequencing a nucleic acid
US20030215821A1 (en) * 1999-04-20 2003-11-20 Kevin Gunderson Detection of nucleic acid reactions on bead arrays
US20040110153A1 (en) * 2002-12-10 2004-06-10 Affymetrix, Inc. Compleixity management of genomic DNA by semi-specific amplification

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20030215821A1 (en) * 1999-04-20 2003-11-20 Kevin Gunderson Detection of nucleic acid reactions on bead arrays
WO2001020039A2 (en) * 1999-09-16 2001-03-22 Curagen Corporation Method of sequencing a nucleic acid
US20040110153A1 (en) * 2002-12-10 2004-06-10 Affymetrix, Inc. Compleixity management of genomic DNA by semi-specific amplification

Also Published As

Publication number Publication date
GB0618046D0 (en) 2006-10-25
WO2008032058A2 (en) 2008-03-20

Similar Documents

Publication Publication Date Title
WO2008032058A3 (en) Method for sequencing a polynucleotide
WO2006007207A3 (en) Methods and devices for nucleic acid sequence determination
WO2012019187A3 (en) Ligation-based detection of genetic variants
WO2007137225A3 (en) Method for indexing nucleic acid sequences for computer based searching
WO2009106308A3 (en) System and method for improved processing of nucleic acids for production of sequencable libraries
WO2010115033A3 (en) Nucleotide repeat expansion-associated polypeptides and uses thereof
WO2009026365A3 (en) Method and system for implementing a dynamic verification value
WO2004104161A3 (en) Methods and systems for identifying naturally occurring antisense transcripts and methods, kits and arrays utilizing same
WO2008002502A3 (en) Devices and systems for creation of dna cluster arrays
GB0400584D0 (en) Nucleic acid chacterisation
DK2415864T3 (en) Oligomerase-2 enzymes (or beta-xylosidase enzymes), nucleic acids encoding them, and methods of producing and using them
WO2010062480A3 (en) Methods and compositions for producing fatty alcohols
WO2012134602A3 (en) Methods and systems for sequencing long nucleic acids
WO2010027497A3 (en) Preparations, compositions, and methods for nucleic acid sequencing
WO2009146180A3 (en) Methods and systems for representation and matching of video content
WO2005010210A3 (en) Determining the sequence of a polynucleotide
PL1794292T3 (en) Alpha-amylase variants stabilized against dimerization and/or multimerization, method for the production thereof, and detergents and cleansers containing these alpha-amylase variants
WO2007127292A3 (en) Tigeycline crystalline forms and processes for preparation thereof
EP3029156A3 (en) Method for the diagnosis and/or prognosis of acute renal damage
EP2562256A3 (en) C5a binding nucleic acids
WO2008135180A3 (en) Pet food and a process for its manufacture
WO2008066816A3 (en) Derivatization or ligaton of peptides
WO2009104094A3 (en) Method of nucleic acid recombination
WO2004110771A3 (en) Security elements and chromophoric security features
WO2010027406A3 (en) Copper layer processing

Legal Events

Date Code Title Description
121 Ep: the epo has been informed by wipo that ep was designated in this application

Ref document number: 07804245

Country of ref document: EP

Kind code of ref document: A2

NENP Non-entry into the national phase

Ref country code: DE

122 Ep: pct application non-entry in european phase

Ref document number: 07804245

Country of ref document: EP

Kind code of ref document: A2