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WO2008009365A3 - Procédé servant à déterminer le taux de méthylation d'un acide nucléique - Google Patents

Procédé servant à déterminer le taux de méthylation d'un acide nucléique Download PDF

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Publication number
WO2008009365A3
WO2008009365A3 PCT/EP2007/006064 EP2007006064W WO2008009365A3 WO 2008009365 A3 WO2008009365 A3 WO 2008009365A3 EP 2007006064 W EP2007006064 W EP 2007006064W WO 2008009365 A3 WO2008009365 A3 WO 2008009365A3
Authority
WO
WIPO (PCT)
Prior art keywords
nucleic acid
base
signal
sequencing
cytosine bases
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/EP2007/006064
Other languages
English (en)
Other versions
WO2008009365A2 (fr
Inventor
Joern Lewin
Ralf Lesche
Matthias Schuster
Dimo Dietrich
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Epigenomics AG
Original Assignee
Epigenomics AG
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority to US12/309,555 priority Critical patent/US20100003680A1/en
Application filed by Epigenomics AG filed Critical Epigenomics AG
Priority to EP07785943A priority patent/EP2044214A2/fr
Publication of WO2008009365A2 publication Critical patent/WO2008009365A2/fr
Publication of WO2008009365A3 publication Critical patent/WO2008009365A3/fr
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6827—Hybridisation assays for detection of mutation or polymorphism
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Immunology (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

L'invention concerne un procédé servant à déterminer quantitativement le taux de méthylation d'un acide nucléique grâce au séquençage. Selon l'invention, le procédé comprend au moins les étapes suivantes consistant à : a) traiter l'acide nucléique avec un réactif chimique ou une solution contenant une enzyme, ce par quoi le comportement d'appariement des bases pour les bases de type cytosines méthylées et/ou les bases de type cytosines non méthylées de l'acide nucléique est modifié de façon à ce que les bases de type cytosines méthylées deviennent distinguables des bases de type cytosines non méthylées ; b) introduire dans l'acide nucléique au moins une base servant à générer un signal de séquençage à utiliser en tant que signal de référence pour la normalisation ; c) séquencer l'acide nucléique, ce par quoi on obtient un signal provenant de chaque base de type cytosine de l'acide nucléique ou un signal provenant de chaque base de type guanine de l'acide nucléique et un signal de référence provenant de ladite ou desdites bases introduites ; et d) normaliser le signal obtenu à partir de chaque base de type cytosine de l'acide nucléique ou le signal obtenu à partir de chaque base de type guanine de l'acide nucléique par rapport au signal de référence provenant de ladite ou desdites bases introduites.
PCT/EP2007/006064 2006-07-18 2007-07-09 Procédé servant à déterminer le taux de méthylation d'un acide nucléique Ceased WO2008009365A2 (fr)

Priority Applications (2)

Application Number Priority Date Filing Date Title
US12/309,555 US20100003680A1 (en) 2006-07-18 2006-07-18 Method For Determining The Methylation Rate of a Nucleic Acid
EP07785943A EP2044214A2 (fr) 2006-07-18 2007-07-09 Procédé servant à déterminer le taux de méthylation d'un acide nucléique

Applications Claiming Priority (6)

Application Number Priority Date Filing Date Title
EP06090125.3 2006-07-18
EP06090125 2006-07-18
EP06120694.2 2006-09-14
EP06120694 2006-09-14
EP07109855.2 2007-06-08
EP07109855 2007-06-08

Publications (2)

Publication Number Publication Date
WO2008009365A2 WO2008009365A2 (fr) 2008-01-24
WO2008009365A3 true WO2008009365A3 (fr) 2008-04-17

Family

ID=38935863

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/EP2007/006064 Ceased WO2008009365A2 (fr) 2006-07-18 2007-07-09 Procédé servant à déterminer le taux de méthylation d'un acide nucléique

Country Status (2)

Country Link
EP (1) EP2044214A2 (fr)
WO (1) WO2008009365A2 (fr)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU2008258270A1 (en) * 2007-06-07 2008-12-11 Simons Haplomics Limited Epigenetic methods
WO2011002029A1 (fr) * 2009-07-03 2011-01-06 国立大学法人東京大学 Procédé pour la détermination de la présence d'une cellule cancéreuse et procédé de détermination d'un pronostic d'un patient présentant un cancer
EP2994545B1 (fr) * 2013-05-10 2019-03-20 University Of Southern California Biomarqueurs de méthylation de l'adn pour le cancer de la vessie
EP4524263A3 (fr) * 2018-10-31 2025-05-28 Guardant Health, Inc. Procédés, compositions et systèmes pour étalonner des dosages de partitionnement épigénétique

Non-Patent Citations (5)

* Cited by examiner, † Cited by third party
Title
HAN ET AL: "DNA methylation mapping by tag-modified bisulfite genomic sequencing", ANALYTICAL BIOCHEMISTRY, ACADEMIC PRESS, SAN DIEGO, CA, US, vol. 355, no. 1, 2 June 2006 (2006-06-02), pages 50 - 61, XP005542202, ISSN: 0003-2697 *
JANG SE JIN ET AL: "Methylation patterns in human androgen receptor gene and clonality analysis", CANCER RESEARCH, vol. 60, no. 4, 15 February 2000 (2000-02-15), pages 864 - 866, XP002467560, ISSN: 0008-5472 *
LEWIN JOERN ET AL: "Quantitative DNA methylation analysis based on four-dye trace data from direct sequencing of PCR amplificates", BIOINFORMATICS (OXFORD), vol. 20, no. 17, 22 November 2004 (2004-11-22), pages 3005 - 3012, XP002467526, ISSN: 1367-4803 *
PAUL C L ET AL: "Cytosine methylation: quantitation by automated genomic sequencing and GENESCAN analysis", BIOTECHNIQUES, INFORMA LIFE SCIENCES PUBLISHING, WESTBOROUGH, MA, US, vol. 21, no. 1, July 1996 (1996-07-01), pages 126 - 133, XP002143107, ISSN: 0736-6205 *
YANG INCHUL ET AL: "Rapid quantification of DNA methylation through dNMP analysis following bisulfite-PCR.", NUCLEIC ACIDS RESEARCH 2006, vol. 34, no. 8, 2006, pages e61, XP002467528, ISSN: 1362-4962 *

Also Published As

Publication number Publication date
EP2044214A2 (fr) 2009-04-08
WO2008009365A2 (fr) 2008-01-24

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