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WO2008007082A3 - Cell growth medium - Google Patents

Cell growth medium Download PDF

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Publication number
WO2008007082A3
WO2008007082A3 PCT/GB2007/002584 GB2007002584W WO2008007082A3 WO 2008007082 A3 WO2008007082 A3 WO 2008007082A3 GB 2007002584 W GB2007002584 W GB 2007002584W WO 2008007082 A3 WO2008007082 A3 WO 2008007082A3
Authority
WO
WIPO (PCT)
Prior art keywords
cell growth
growth medium
feeder
stem cells
relates
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/GB2007/002584
Other languages
French (fr)
Other versions
WO2008007082A2 (en
Inventor
Miho Furue
Peter Andrews
Tetsuji Okamoto
Denry J Sato
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
University of Sheffield
Original Assignee
University of Sheffield
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by University of Sheffield filed Critical University of Sheffield
Priority to AU2007274065A priority Critical patent/AU2007274065A1/en
Priority to JP2009518958A priority patent/JP5227318B2/en
Priority to EP07766180A priority patent/EP2038404A2/en
Priority to CA002657539A priority patent/CA2657539A1/en
Publication of WO2008007082A2 publication Critical patent/WO2008007082A2/en
Publication of WO2008007082A3 publication Critical patent/WO2008007082A3/en
Priority to GB0823575A priority patent/GB2452456A/en
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0603—Embryonic cells ; Embryoid bodies
    • C12N5/0606—Pluripotent embryonic cells, e.g. embryonic stem cells [ES]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/05—Inorganic components
    • C12N2500/10—Metals; Metal chelators
    • C12N2500/20—Transition metals
    • C12N2500/24—Iron; Fe chelators; Transferrin
    • C12N2500/25—Insulin-transferrin; Insulin-transferrin-selenium
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/30—Organic components
    • C12N2500/38—Vitamins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/30—Organic components
    • C12N2500/44—Thiols, e.g. mercaptoethanol
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/30—Organic components
    • C12N2500/46—Amines, e.g. putrescine
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/90—Serum-free medium, which may still contain naturally-sourced components
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/115—Basic fibroblast growth factor (bFGF, FGF-2)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/90—Polysaccharides
    • C12N2501/91—Heparin

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Gynecology & Obstetrics (AREA)
  • Biotechnology (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Developmental Biology & Embryology (AREA)
  • Chemical & Material Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Reproductive Health (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Cell Biology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

The invention relates to a method to culture primate embryonic stem cells in feeder and serum free conditions.
PCT/GB2007/002584 2006-07-12 2007-07-10 Cell growth medium Ceased WO2008007082A2 (en)

Priority Applications (5)

Application Number Priority Date Filing Date Title
AU2007274065A AU2007274065A1 (en) 2006-07-12 2007-07-10 Cell growth medium
JP2009518958A JP5227318B2 (en) 2006-07-12 2007-07-10 Cell growth medium
EP07766180A EP2038404A2 (en) 2006-07-12 2007-07-10 Cell growth medium
CA002657539A CA2657539A1 (en) 2006-07-12 2007-07-10 Cell growth medium
GB0823575A GB2452456A (en) 2006-07-12 2008-12-29 Cell growth medium

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
GBGB0613756.6A GB0613756D0 (en) 2006-07-12 2006-07-12 Cell culture medium
GB0613756.6 2006-07-12

Publications (2)

Publication Number Publication Date
WO2008007082A2 WO2008007082A2 (en) 2008-01-17
WO2008007082A3 true WO2008007082A3 (en) 2008-03-06

Family

ID=36955441

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/GB2007/002584 Ceased WO2008007082A2 (en) 2006-07-12 2007-07-10 Cell growth medium

Country Status (7)

Country Link
EP (1) EP2038404A2 (en)
JP (1) JP5227318B2 (en)
CN (1) CN101490243A (en)
AU (1) AU2007274065A1 (en)
CA (1) CA2657539A1 (en)
GB (2) GB0613756D0 (en)
WO (1) WO2008007082A2 (en)

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9018010B2 (en) 2009-11-12 2015-04-28 Technion Research & Development Foundation Limited Culture media, cell cultures and methods of culturing pluripotent stem cells in an undifferentiated state
US9040297B2 (en) 2006-08-02 2015-05-26 Technion Research & Development Foundation Limited Methods of expanding embryonic stem cells in a suspension culture
US9404079B2 (en) 2005-08-29 2016-08-02 Technion Research & Development Foundation Limited Media for culturing stem cells

Families Citing this family (32)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9688956B2 (en) 2008-06-05 2017-06-27 National Cheng Kung University Method for preserving proliferation and differentiation potential of mesenchymal stem cells
JP5763535B2 (en) 2008-06-24 2015-08-12 バイオアクティブ サージカル インコーポレイテッド Surgical sutures incorporating stem cells or other bioactive materials
CN102176882A (en) 2008-08-07 2011-09-07 生物活性外科公司 Stem cell capture and immobilization coatings for medical devices and implants
CN104130976A (en) * 2008-12-17 2014-11-05 斯克里普斯研究所 Generation and maintenance of stem cells
RU2012106760A (en) * 2009-07-21 2013-08-27 Трансген Са Enzymatic composition for enzymatic cleavage of chicken embryo
CN101812480B (en) * 2009-11-16 2013-04-10 西北农林科技大学 Method utilizing transcription factor to transfect bovine body cell into induced pluripotent stem cell
JP5714267B2 (en) * 2010-08-26 2015-05-07 日本メナード化粧品株式会社 Stem cell undifferentiation maintenance agent and proliferation promoter
JP6148429B2 (en) 2011-01-31 2017-06-14 協和発酵バイオ株式会社 Method for culturing human pluripotent stem cells
CN102586176B (en) * 2012-01-11 2013-05-08 中国科学院生物物理研究所 Novel animal source-free and feed layer-free human pluripotent stem cell culture system
GB201211873D0 (en) * 2012-07-04 2012-08-15 Univ Edinburgh Cell culture
CN104955939B (en) * 2013-01-31 2018-07-27 味之素株式会社 The cultural method of the undifferentiated maintenance proliferation of stabilization for carrying out multipotent stem cells
KR102229266B1 (en) * 2013-05-30 2021-03-19 아지노모토 가부시키가이샤 Medium for culturing stem cells
WO2014208295A1 (en) * 2013-06-28 2014-12-31 株式会社カネカ Screening process for pluripotent stem cell propagation promoting factor
WO2015042356A1 (en) * 2013-09-19 2015-03-26 The United States Of America, As Represented By The Secretary, Department Of Health & Human Services Chemically defined culture medium for stem cell maintenance and differentiation
CN104357379B (en) * 2014-09-30 2017-08-08 刘兴宇 Stem cell media
CN106032527B (en) * 2015-03-17 2019-08-13 广州市搏克肿瘤研究所 A feeder-free culture medium for human pluripotent stem cells that tolerates low density
CN105907705B (en) * 2016-06-28 2020-01-24 广州市搏克生物技术有限公司 A kind of pluripotent stem cell culture medium
AU2018209127B2 (en) 2017-01-23 2021-10-21 Stemcell Technologies Canada Inc. Media and methods for enhancing the survival and proliferation of stem cells
CN106754715A (en) * 2017-02-13 2017-05-31 四川新生命干细胞科技股份有限公司 A kind of trophoblastic preparation method for candidate stem cell culture
CN106754652B (en) * 2017-03-06 2019-04-02 广州润虹医药科技股份有限公司 IPS cell differentiation at ectoderm progenitor cells serum-free induced medium and abductive approach
CN106754657B (en) * 2017-03-28 2022-07-22 北京赛斯达生物技术有限公司 Serum-free medium for monkey embryonic stem cells
PL3436568T3 (en) * 2017-05-31 2023-11-20 Promocell Gmbh Culture medium for pluripotent stem cells
CN110157662A (en) * 2018-02-15 2019-08-23 郭永珍 A kind of cell additive and preparation method thereof
WO2019177420A1 (en) * 2018-03-16 2019-09-19 사회복지법인 삼성생명공익재단 Medium composition comprising fgf-17 as effective ingredient for promotion of stem cell proliferation and method for promotion of stem cell proliferation by using same
CA3104838A1 (en) * 2018-06-27 2020-01-02 Ajinomoto Co., Inc. Additive for culturing stem cells, culturing medium, and culturing method
CN110684716A (en) * 2018-07-08 2020-01-14 郭永珍 Preparation method of cell additive and product thereof
CN109486766B (en) * 2018-11-26 2021-05-07 中山大学 Lacrimal gland stem cell, culture system and culture method of lacrimal gland stem cell
CN111484970B (en) * 2020-04-30 2022-09-16 广州再生医学与健康广东省实验室 A serum-free feeder-free embryo and pluripotent stem cell culture medium with low protein content
WO2023150293A2 (en) * 2022-02-03 2023-08-10 Steakholder Foods Ltd. Accelerated myotube formation
CN116210647B (en) * 2023-02-03 2024-12-13 威奥福生物科技(宁波)有限公司 A method for multiplying white-feathered broiler chickens using primordial germ cell stem cell lines
CN119144559A (en) * 2023-06-14 2024-12-17 基可生医股份有限公司 Allograft colloid set and its use method
CN118389410B (en) * 2024-06-27 2024-10-22 中国农业大学 Composition and method for regulating in vitro embryo DNA methylation modification and application of composition and method in improving embryo development efficiency and quality

Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1998030679A1 (en) * 1997-01-10 1998-07-16 Life Technologies, Inc. Embryonic stem cell serum replacement
WO2003029443A1 (en) * 2001-09-28 2003-04-10 Es Cell International Pte Ltd Methods of derivation and propagation of undifferentiated human embryonic stem (hes) cells on feeder-free matrices and human feeder layers
WO2004111210A1 (en) * 2003-06-12 2004-12-23 Yeda Research & Development Co.Ltd Enhancement of oligodendrocyte differentiation
WO2006017370A2 (en) * 2004-07-13 2006-02-16 Geron Corporation Medium for growing human embryonic stem cells
WO2006029197A1 (en) * 2004-09-08 2006-03-16 Wisconsin Alumni Research Foundation Medium and culture of embryonic stem cells
WO2006036925A1 (en) * 2004-09-28 2006-04-06 Wicell Research Institute, Inc. Cultivation of primate embryonic stem cells

Family Cites Families (1)

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Publication number Priority date Publication date Assignee Title
EP1698690B1 (en) * 2003-12-26 2010-04-28 Makoto Asashima Basal medium for es cell culturing

Patent Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1998030679A1 (en) * 1997-01-10 1998-07-16 Life Technologies, Inc. Embryonic stem cell serum replacement
WO2003029443A1 (en) * 2001-09-28 2003-04-10 Es Cell International Pte Ltd Methods of derivation and propagation of undifferentiated human embryonic stem (hes) cells on feeder-free matrices and human feeder layers
WO2004111210A1 (en) * 2003-06-12 2004-12-23 Yeda Research & Development Co.Ltd Enhancement of oligodendrocyte differentiation
WO2006017370A2 (en) * 2004-07-13 2006-02-16 Geron Corporation Medium for growing human embryonic stem cells
WO2006029197A1 (en) * 2004-09-08 2006-03-16 Wisconsin Alumni Research Foundation Medium and culture of embryonic stem cells
WO2006036925A1 (en) * 2004-09-28 2006-04-06 Wicell Research Institute, Inc. Cultivation of primate embryonic stem cells

Non-Patent Citations (4)

* Cited by examiner, † Cited by third party
Title
CALDWELL MAEVE A ET AL: "HEPARIN, BUT NOT OTHER PROTEOGLYCANS POTENTIATES THE MITOGENIC EFFECTS OF FGF-2 ON MESENCEPHALIC PRECURSOR CELLS", EXPERIMENTAL NEUROLOGY, ACADEMIC PRESS, NEW YORK, NY, US, vol. 152, no. 1, July 1998 (1998-07-01), pages 1 - 10, XP009073850, ISSN: 0014-4886 *
LI Y ET AL: "Expansion of human embryonic stem cells in defined serum-free medium devoid of animal-derived products", BIOTECHNOLOGY AND BIOENGINEERING, WILEY & SONS, HOBOKEN, NJ, US, vol. 91, no. 6, 20 September 2005 (2005-09-20), pages 688 - 698, XP002351170, ISSN: 0006-3592 *
REN WENWEN ET AL: "bFGF and heparin but not laminin are necessary factors in the mediums that affect NSCs differentiation into cholinergic neurons.", NEUROLOGICAL RESEARCH JAN 2006, vol. 28, no. 1, January 2006 (2006-01-01), pages 87 - 90, XP002462651, ISSN: 0161-6412 *
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Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9404079B2 (en) 2005-08-29 2016-08-02 Technion Research & Development Foundation Limited Media for culturing stem cells
US9040297B2 (en) 2006-08-02 2015-05-26 Technion Research & Development Foundation Limited Methods of expanding embryonic stem cells in a suspension culture
US9018010B2 (en) 2009-11-12 2015-04-28 Technion Research & Development Foundation Limited Culture media, cell cultures and methods of culturing pluripotent stem cells in an undifferentiated state

Also Published As

Publication number Publication date
JP2009542247A (en) 2009-12-03
GB0823575D0 (en) 2009-01-28
WO2008007082A2 (en) 2008-01-17
CA2657539A1 (en) 2008-01-17
AU2007274065A1 (en) 2008-01-17
EP2038404A2 (en) 2009-03-25
JP5227318B2 (en) 2013-07-03
GB2452456A (en) 2009-03-04
CN101490243A (en) 2009-07-22
GB0613756D0 (en) 2006-08-23

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