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WO2008002329A3 - Isolation and expansion of animal cells in cell cultures - Google Patents

Isolation and expansion of animal cells in cell cultures Download PDF

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Publication number
WO2008002329A3
WO2008002329A3 PCT/US2006/049500 US2006049500W WO2008002329A3 WO 2008002329 A3 WO2008002329 A3 WO 2008002329A3 US 2006049500 W US2006049500 W US 2006049500W WO 2008002329 A3 WO2008002329 A3 WO 2008002329A3
Authority
WO
WIPO (PCT)
Prior art keywords
cells
stem
cell
expansion
methods
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/US2006/049500
Other languages
French (fr)
Other versions
WO2008002329A2 (en
Inventor
Scheffer Tseng
Hua He
Wei Li
Ying-Ting Chen
Yasutaka Hayashida
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
BioTissue Holdings Inc
Original Assignee
TissueTech Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from US11/476,376 external-priority patent/US20070010008A1/en
Application filed by TissueTech Inc filed Critical TissueTech Inc
Publication of WO2008002329A2 publication Critical patent/WO2008002329A2/en
Publication of WO2008002329A3 publication Critical patent/WO2008002329A3/en
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0618—Cells of the nervous system
    • C12N5/0621—Eye cells, e.g. cornea, iris pigmented cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0603—Embryonic cells ; Embryoid bodies
    • C12N5/0605—Cells from extra-embryonic tissues, e.g. placenta, amnion, yolk sac, Wharton's jelly
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/90—Serum-free medium, which may still contain naturally-sourced components

Landscapes

  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biomedical Technology (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • Reproductive Health (AREA)
  • Developmental Biology & Embryology (AREA)
  • Gynecology & Obstetrics (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Cell Biology (AREA)
  • Pregnancy & Childbirth (AREA)
  • Ophthalmology & Optometry (AREA)
  • Neurology (AREA)
  • Neurosurgery (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)

Abstract

Described are methods for isolating/purifying and expanding animal stem cells and stem-celHike cells. Isolation methods include conditions comprising preferentially digesting non-stem cells and non-stem-cell-like cells in a population and preferentially adhering stem cells and stem-cell-like cells in a population. Expansion methods include culturing such cells under conditions comprising modulation of TGF-β signaling, inhibition of cell signaling mediated by p38 MAP kinase using small molecular weight inhibitors, expansion of the cells on human amniotic epithelial cells as feeder layers, control of cell seeding density, control of levels Of Ca2+ in the culture media, rapid adhesion on a substrate or by a combination of such conditions. More particularly, what is disclosed relates to methods and systems for expanding animal cells in ex vivo cell cultures, while preventing cellular differentiation, and selectively enriching stem cells. The embodiments also disclose a culture system for ex vivo expansion of limbal epithelial cells or mesenchymal cells, as well as surgical grafts made there from.
PCT/US2006/049500 2006-05-17 2006-12-28 Isolation and expansion of animal cells in cell cultures Ceased WO2008002329A2 (en)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US80149106P 2006-05-17 2006-05-17
US60/801,491 2006-05-17
US11/476,376 2006-06-28
US11/476,376 US20070010008A1 (en) 2005-06-29 2006-06-28 Ex vivo expansion of primary animal cells

Publications (2)

Publication Number Publication Date
WO2008002329A2 WO2008002329A2 (en) 2008-01-03
WO2008002329A3 true WO2008002329A3 (en) 2008-07-17

Family

ID=38846144

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2006/049500 Ceased WO2008002329A2 (en) 2006-05-17 2006-12-28 Isolation and expansion of animal cells in cell cultures

Country Status (1)

Country Link
WO (1) WO2008002329A2 (en)

Families Citing this family (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9234179B2 (en) 2009-12-18 2016-01-12 Shanghai Icell Biotechnology Co., Ltd. Materials and methods for generating pluripotent stem cells
EP3553169B1 (en) * 2011-12-28 2021-11-03 Kyoto Prefectural Public University Corporation Normalization of culture of corneal endothelial cells
US9664671B2 (en) 2012-07-24 2017-05-30 Nissan Chemical Industries, Ltd. Culture medium composition and method of culturing cell or tissue using thereof
US10017805B2 (en) 2012-08-23 2018-07-10 Nissan Chemical Industries, Ltd. Enhancing ingredients for protein production from various cells
TWI612135B (en) * 2012-08-23 2018-01-21 日產化學工業股份有限公司 Method for manufacturing culture medium for animal cell, culture medium for animal cell, method for manufacturing protein, and method for promoting protein production of animal cell in culture
WO2014158089A1 (en) * 2013-03-28 2014-10-02 Ge Healthcare Bio-Sciences Ab Method for cell culture
US9944895B2 (en) 2013-03-28 2018-04-17 Ge Healthcare Bio-Sciences Ab Method for cell culture
CN119265109B (en) * 2024-12-10 2025-04-22 华羊生物技术股份有限公司 Culture medium for multidirectional induced differentiation of amniotic epithelial stem cells and preparation method thereof

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2007002900A1 (en) * 2005-06-29 2007-01-04 Tissue Tech, Inc. Ex vivo expansion of primary animal cells

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2007002900A1 (en) * 2005-06-29 2007-01-04 Tissue Tech, Inc. Ex vivo expansion of primary animal cells

Non-Patent Citations (5)

* Cited by examiner, † Cited by third party
Title
BONFIELD W. ET AL.: "Designing porous scaffolds for tissue engineering", PHILOSOPHICAL TRANSACTIONS. SERIES A, MATHEMATICAL, PHYSICAL, AND ENGINEERING SCIENCES, vol. 364, no. 1838, January 2006 (2006-01-01), pages 227 - 232 *
KAWAKITA T. ET AL.: "Keratocan Expression of Murine Keratocytes Is Maintained on Amniotic Membrane by Down-regulating Transforming Growth Factor-beta Signaling", THE JOURNAL OF BIOLOGICAL CHEMISTRY, vol. 280, no. 29, 2005, pages 27058 - 27092 *
KAWAKITA T. ET AL.: "Preservation and Expansion of the Primate Keratocyte Phenotype by Downregulating TGF-beta Signaling in a Low-Calcium, Serum-Free Medium", INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE, vol. 47, no. 5, May 2006 (2006-05-01), pages 1918 - 1927 *
YAM H.F. ET AL.: "Growth Factor Changes in Ex Vivo Expansion of Human Limbal Epithelial Cells on Human Amniotic Membrane", CORNEA, vol. 21, no. 1, 2002, pages 101 - 105 *
YEH L.K. ET AL.: "Soluble Lumican Glycoprotein Purified from Human Amniotic Membrane Promotes Corneal Epithelial Wound Healing", INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE, vol. 46, no. 2, 2005, pages 479 - 486 *

Also Published As

Publication number Publication date
WO2008002329A2 (en) 2008-01-03

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