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WO2008002004A1 - Centrifugeuse et procédé de centrifugation - Google Patents

Centrifugeuse et procédé de centrifugation Download PDF

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Publication number
WO2008002004A1
WO2008002004A1 PCT/KR2007/000980 KR2007000980W WO2008002004A1 WO 2008002004 A1 WO2008002004 A1 WO 2008002004A1 KR 2007000980 W KR2007000980 W KR 2007000980W WO 2008002004 A1 WO2008002004 A1 WO 2008002004A1
Authority
WO
WIPO (PCT)
Prior art keywords
container
rotator
centrifuge
opening portion
layers
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/KR2007/000980
Other languages
English (en)
Inventor
Jun-Seok Lee
Hee-Young Lee
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
MEDIKAN Inc
YAHOH PROGRESSIVE IDEA AREA
Original Assignee
MEDIKAN Inc
YAHOH PROGRESSIVE IDEA AREA
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from KR1020060111265A external-priority patent/KR100772970B1/ko
Application filed by MEDIKAN Inc, YAHOH PROGRESSIVE IDEA AREA filed Critical MEDIKAN Inc
Publication of WO2008002004A1 publication Critical patent/WO2008002004A1/fr
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

Links

Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B04—CENTRIFUGAL APPARATUS OR MACHINES FOR CARRYING-OUT PHYSICAL OR CHEMICAL PROCESSES
    • B04B—CENTRIFUGES
    • B04B5/00—Other centrifuges
    • B04B5/04—Radial chamber apparatus for separating predominantly liquid mixtures, e.g. butyrometers
    • B04B5/0407—Radial chamber apparatus for separating predominantly liquid mixtures, e.g. butyrometers for liquids contained in receptacles
    • B04B5/0414—Radial chamber apparatus for separating predominantly liquid mixtures, e.g. butyrometers for liquids contained in receptacles comprising test tubes
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5021—Test tubes specially adapted for centrifugation purposes
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B04—CENTRIFUGAL APPARATUS OR MACHINES FOR CARRYING-OUT PHYSICAL OR CHEMICAL PROCESSES
    • B04B—CENTRIFUGES
    • B04B11/00—Feeding, charging, or discharging bowls
    • B04B11/04—Periodical feeding or discharging; Control arrangements therefor
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B04—CENTRIFUGAL APPARATUS OR MACHINES FOR CARRYING-OUT PHYSICAL OR CHEMICAL PROCESSES
    • B04B—CENTRIFUGES
    • B04B7/00—Elements of centrifuges
    • B04B7/08—Rotary bowls
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/02—Adapting objects or devices to another
    • B01L2200/026—Fluid interfacing between devices or objects, e.g. connectors, inlet details
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/04—Moving fluids with specific forces or mechanical means
    • B01L2400/0475—Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure
    • B01L2400/0487—Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure fluid pressure, pneumatics
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B04—CENTRIFUGAL APPARATUS OR MACHINES FOR CARRYING-OUT PHYSICAL OR CHEMICAL PROCESSES
    • B04B—CENTRIFUGES
    • B04B7/00—Elements of centrifuges
    • B04B7/02—Casings; Lids
    • B04B2007/025—Lids for laboratory centrifuge rotors

Definitions

  • the present invention relates to a centrifuge and a centrifuging method, and more particularly, to a centrifuge and a centrifuging method in which, when centrifuging is performed by rotation of a rotator, a layer of a material having low fluid resistance and low specific gravity and a layer having high fluid resistance and high specific gravity among the layers separated by centrifuging can be classified and collected accurately and easily by opening an opening portion of a container.
  • the centrifuge and the centrifuging method according to the present invention can be applied to all kinds of materials that can move downward along an inclined surface, that is, both to liquids and solids such as liquid, powder, gel, a mixture of liquid and solid, colloids, and solids that are nearly spherical.
  • a centrifuge is an apparatus for separating materials using centrifugal force that is generated when an object is rotated. Centrifuges can be classified according to an amount of a sample to be centrifuged, the rotation speed, the rotor, etc.
  • a centrifuge is used in biotechnology to separate cells mixed in a liquid or a material having greater weight and adhesive force than a liquid. Recently, intensive research on adult stem cells has been conducted, and a centrifugal method of separating a small amount of stem cells from a large amount of adult tissues (fat, bone- marrow, etc.) is currently being researched.
  • centrifugal force required for centrifuging cells is 100 G maximum, usually lower than this.
  • G denotes a gravitational constant. Since a centrifugal force acts against in a centrifuge, a centrifugal force will be represented using the unit 'G.
  • a complex fluid such as blood is centrifuged using a centrifuge in a biotechnology laboratory, the fluid is divided into several layers according to the specific gravity of each element of the complex fluid.
  • a complex fluid refers to a liquid that includes semisolids such as fine solid components or gel and has a broader range than a polymer; hereinafter, it will be referred to as 'fluid'.
  • each centrifuged layer of fluid is stacked in a test tube and can be discriminated visually; however, it is difficult to separate the layers physically.
  • Layers of a fluid centrifuged by a centrifuge are generally separated by hand.
  • separating layers of a centrifuged fluid is an onerous task, and moreover, loss of the centrifuged material may be caused and thus purity of the separated material cannot be secured.
  • the present invention provides a centrifuge and a centrifugal method in which a material is separated by a centrifugal force and layers of the separated material are classified and collected accurately and easily.
  • the present invention also provides a centrifuge and a centrifugal method in which stem cells are accurately classified and collected from layers of a material separated by centrifuge.
  • the present invention provides a centrifuge and a centrifugal method in which a layer of a material having a low specific gravity among the layers of the material is separated by a centrifugal force, and layers of the separated material are classified and collected accurately and easily.
  • the centrifuge and the centrifuging method according to the present invention are for centrifuging a complex fluid (a liquid that includes semisolids such as fine solid components or gel and has a broader range than a polymer; hereinafter referred to as 'fluid') such as blood extracted from a human body, fat, and the like.
  • a complex fluid a liquid that includes semisolids such as fine solid components or gel and has a broader range than a polymer; hereinafter referred to as 'fluid'
  • stem cells contained in a small amount in a complex fluid such as fat extracted from a human body can be centrifuged, and the stem cells moved by the operation of centrifugal force are accumulated in a container that is detachably installed, and thus the stem cells can be accurately and easily collec ted without any additional separation process or washing process.
  • the centrifuge and the centrifuging method according to the present invention can be applied to all kinds of materials that can move downward along an inclined surface, that is, both to liquids and solids such as liquid, powder, gel, a mixture of liquid and solid, colloids, and solids that are nearly spherical.
  • the present invention provides a centrifuge and a centrifuging method in which a container is inclined at a predetermined angle with respect to a rotation axis of the centrifuge, and while the centrifuge is rotated, a stopper portion of the container opens or closes an opening portion of the container. Accordingly, the present invention provides a centrifuge and a centrifuging method whereby centrifuging can be performed and segregated layers can be classified accurately and easily and be collected.
  • fluid resistance refers to the complex characteristic of a fluid, whereby the fluid is resistant to flow due to adhesive force, cohesive force, viscosity, etc.
  • the present invention provides a centrifuge and a centrifuging method using the centrifuge that can accurately separate and collect layers of stem cells in the centr ifuge without any additional separation or washing process based on the characteristic of stem cells in a complex fluid such as fat tissue extracted from a human body, which has higher fluid resistance compared to other elements contained in a fluid of fatty tissue and is affected by a relatively greater centrifugal force at the same rotation speed than other materials.
  • the centrifuge When the centrifuge performs centrifuging according to the present invention, the centrifuge is rotated at a maximum speed required for centrifuging, that is, at a first speed for a predetermined period of time to centrifuge a material into segregated layers.
  • the speed of the centrifuge needs to be slowed down from the first speed to a second speed that is lower than the first speed and the centrifuge is rotated again for a predetermined period of time.
  • the container is installed in the centrifuge such that an opening portion of the container directs downward and is inclined at a predetermined angle. Accordingly, while the centrifuge is being rotated at the second speed, and the opening portion of the container is opened, some layers formed of a material having low fluid resistance and low specific gravity in the container can flow down through the opening portion of the container.
  • the second speed is determined by the characteristic of the material to be centrifuged, and particularly, by the fluid resistance.
  • layers at the outermost edge such as stem cells among segregated layers formed in the container are maintained inside the container by being affected by a centrifugal force that is greater than gravity; however, the other segregated layers may flow down by being affected by a centrifugal force that is less than gravity.
  • a critical point at which some of the segregated layers are discharged outside of the container can be determined by adjusting the second speed according to the segregated layers to be centrifuged.
  • a centrifuge comprising: a rotator rotating around a rotation axis; a container into which a material is delivered and comprising an opening portion, inclined at a predetermined angle with respect to a rotation axis of the rotator such that the opening portion is directed downward, and coupled to the rotator; and a stopper portion that is coupled to the opening portion and closes the opening portion, and when the speed of the rotator reaches a predetermined speed and thus the material is centrifuged, opens the opening portion such that the material in the container flows outward.
  • the stopper portion may comprise a valve which is operated according to an external signal and thus opens or closes the opening portion.
  • a receiving unit which receives a material when the stopper portion is opened may be formed in the rotator.
  • a connecting portion connecting the container to the outside may be connected to the stopper portion.
  • the connecting portion may comprise at least one discharge pipe discharging the material of the container.
  • the connecting portion may further comprise an inflow pipe delivering a fluid into the container from the outside.
  • a method of centrifuging comprising: (a) delivering a material into a container; (b) detachably coupling the container to a rotator at a predetermined angle with respect to a rotation axis of the rotator such that an opening portion of the container is directed downward; (c) centrifuging the material in the container by rotating the rotator; (d) opening the stopper portion coupled to the opening portion while continuously rotating the rotator to discharge some layers of the centrifuged material to the outside of the container; (e) closing the stopper portion; (f) stopping the rotator; and (g) separating the container from the rotator to collect the centrifuged material.
  • a discharge pipe connected to the container via the stopper portion may be opened to discharge some layers of the centrifuged material.
  • the discharge pipe may be connected to a pump, and some layers of the centrifuged material may be discharged to the outside of the container by pressure produced by the pump.
  • an inflow pipe connected to the container via the stopper portion may be opened to deliver an external fluid into the container to maintain the pressure of the container regular.
  • the rotator may be rotated at a speed reduced compared to the speed in (c), and the stopper portion may be opened to discharge some layers of the centrifuged material.
  • centrifuging is performed with a container being maintained at a predetermined angle with respect to the rotation axis of the centrifuge, and an opening portion of the container coupled to a rotator and directing downward.
  • a stopper portion coupled to the container opens or closes the opening portion of the container.
  • FlG. 1 is a perspective view illustrating a centrifuge according to an embodiment of the present invention
  • FlG. 2 is a side cross-sectional view illustrating the centrifuge of FlG. 1, according to an embodiment of the present invention
  • FlG. 3 is a schematic view illustrating forces applied to a material when a centrifugal force is not present in a centrifuge according to an embodiment of the present invention
  • FlG. 4A schematically illustrates forces of FlG. 3 changed to a state where a high centrifugal force is applied
  • FlG. 4B schematically illustrates forces of FlG. 3 changed to a state where a low centrifugal force is applied
  • FlG. 5 is a side view of a portion of a centrifuge, illustrating a container before centrifuging starts according to an embodiment of the present invention
  • FlG. 6 is a side view of a portion of a centrifuge, illustrating the container of FlG. 5 in which a portion of separated material is collected;
  • FlG. 7 is a flowchart illustrating a centrifuging method according to an embodiment of the present invention.
  • FlG. 1 is a perspective view illustrating a centrifuge according to an embodiment of the present invention.
  • FlG. 2 is a side cross-sectional view illustrating the centrifuge of FIG. 1.
  • the centrifuge illustrated in FIGS. 1 and 2 is an apparatus that separates a material by being rotated around a rotation axis O.
  • the centrifuge according to the current embodiment of the present invention includes a rotator 10, a container 20 coupled to the rotator 10 at a predetermined angle ⁇ , and a stopper portion 30 opening or closing an opening portion 21 of the container 20.
  • the rotator 10 rotates around the rotation axis O and supports the container 20.
  • the rotator 10 is disposed inside a casing 11 and is coupled to a driving motor 12 to rotate in the casing 11 for centrifuging.
  • a cover 13 covering the rotator 11 and the container 20 is coupled to the casing 11.
  • a receiving unit 15 is formed in the rotator 10.
  • the receiving unit 15 receives the material flowing down from the container 20.
  • a plurality of containers 20 may be coupled to the rotator 10.
  • the container 20 is coupled to the rotator 10 such that the opening portion 21 of the container 20 is directed downward. Accordingly, the container 20 is inclined at a predetermined angle with respect to the rotation axis O.
  • An angle ⁇ between an axis P in a length direction of the container 20 and the rotation axis O can be determined in consideration of gravity, centrifugal force, and a force by fluid resistance applied to the material so that the material can flow down when a layer of the material separated by the centrifuging is discharged outside the container 20.
  • the container 20 may be an ordinary test tube into which a material to be centrifuged is received.
  • the opening portion 21 is formed in the container 20, and an end portion of the opening portion 21 away from the rotator 10 has a horizontal cross- section becoming reduced downwardly.
  • a stopper portion 30 is coupled to the container 20.
  • the stopper portion 30 is coupled to the opening portion 21 of the container 20 and opens or closes the opening portion 21.
  • One side of the stopper portion 30 is coupled to the opening portion 21 of the container 20, and another side of the stopper portion 30 is opened toward the receiving unit 15 of the rotator 10.
  • the opening portion 21 of the container 20 is opened toward the receiving unit 15 of the rotator 10.
  • the stopper portion 30 may include a valve 31 that is operated according to external signals.
  • the valve 31 is connected to a controller 33 via a signal line 32 installed in the rotator 10 and is operated by a driving signal supplied by the controller 33. Accordingly, when the opening portion 21 is opened by the valve 31, the material in the container 20 can flow downward, and when the opening portion 21 is closed, the material stays inside the container 20.
  • the valve 31 may also be operated by external signals wirelessly instead of by being connected to the outside via the signal line 32.
  • the centrifuge according to the current embodiment of the present invention is operated as follows.
  • a sample to be centrifuged is filled into the container 20, and the container 20 is coupled to the rotator 10.
  • the axis P in a length direction of the container 20 is maintained at a predetermined angle ⁇ with respect to the rotation axis O.
  • the stopper portion 30 closes the opening portion 21 of the container 20 such that the material inside the container 20 does not flow outside.
  • the driving motor 12 connected to the rotator 10 is driven, the rotator 10 rotates around the rotation axis O.
  • the container 20 is coupled to the rotator 10 and thus the container 20 is rotated together with the rotator 10.
  • the rotation speed of the rotator 10 for performing centrifuging is referred to as a first speed.
  • a layer formed of a material having high specific gravity and high fluid resistance is formed relatively far away from the center of the rotation axis O and a layer formed of a material having low specific gravity and low fluid resistance such as free oil, fat, and water is formed relatively near the rotation axis O.
  • the layer relatively far away from the center of the rotation axis O is a material having high specific gravity such as stem cells.
  • the stem cells are pressurized and adhered to a wall surface of an end of the container 20 due to the adhesive force of the stem cells themselves and the centrifugal force.
  • the rotator 10 rotates at a second speed that is lower than the first speed. That is, the rotation speed of the rotator 10 is reduced to a second speed which is a critical speed that is predetermined so that some of the centrifuged layers can flow downward due to gravity and so that at the same time the stem cells are maintained inside the container 20.
  • the second speed can be set as 400 rpm.
  • the centrifuge according to the current embodiment of the present invention can be applied to all kinds of materials that can move downward along an inclined surface, that is, both to liquids and solids such as liquid, powder, gel, a mixture of liquid and solid, colloids, and solids that are nearly spherical.
  • FIG. 3 is a schematic view illustrating forces applied to a material provided that a centrifugal force is not generated in a centrifuge according to an embodiment of the present invention.
  • the container is fixed to form an angle ⁇ with respect to a horizontal surface, thereby having an inclined surface As with respect to gravity.
  • the centrifuge does not rotate and thus no centrifugal force is applied.
  • Only gravity G is applied to the material, and a component Gs of the gravity G in the direction of the inclined surface As makes the material flow downward along the inclined surface As of the container.
  • a frictional force Fs due to a fluid resistance between the material and the inclined surface As of the container is applied in an opposite direction to the flow of the material, thereby resisting the downward flow of the material.
  • FIG. 4A schematically illustrates forces of FIG. 3 changed to a state where a high centrifugal force is applied.
  • the speed of the rotator needs to be reduced so that the centrifugal force C may be decreased, or the inclined surface As needs to be more inclined so that the component Gs of the gravity G in the direction of the inclined surface As of the container may increase.
  • the angle of the container is fixed with respect to the rotator, and thus the inclined surface cannot be further inclined. Accordingly, the rotation speed needs to be changed.
  • FIG. 4B schematically illustrates forces of FIG. 3 changed to a state where a low centrifugal force is applied
  • the total force of the force of the centrifugal force C on the inclined surface As of the container and the friction force Fs is applied opposite to the component Gs of the gravity G in the direction of the inclined surface As. Accordingly, when the force Ts on the inclined surface As of the container is greater than the frictional force Fs operating as a resistance, the material can move downward.
  • the rotator is rotated at the same RPM, that is, at the same rotation speed, the greater the rotation radius, the greater the centrifugal force. Accordingly, when the rotation speed of the rotator is reduced, the layer nearest to the rotation axis starts to flow down first. The greater the specific gravity of a layer is, the greater the rotation radius of the layer is. Thus, while the speed of the rotator is reduced, a material positioned at a relatively large rotation radius flows later than a material positioned at a relatively smaller rotation radius. Accordingly, it is easier to obtain a material having a greater specific gravity. Also, a force that is created to move the material upward by gravity and centrifugal force is called an elevating force, and the material moves upward or downward along the inclined surface of the container depending on the size of the elevating force.
  • frictional force is a parameter concerning fluid resistance. Forces disturbing the complex flow of a fluid are determined by various parameters. Examples of the parameters are viscosity and cohesive force (surface tension) in the case of liquids, and mobility, friction in the case of solids, and adhesiveness of semisolids (gel) or mixed material. Such forces are types of frictional force. The total of all of such forces can be expressed as anti- flow force or anti-falling force.
  • a material having a large in specific gravity and high flow resistance can be easily separated from a material having a low specific gravity and low flow resistance.
  • a cell which is an object material to be obtained, satisfies both characteristics such as a large specific gravity and high flow resistance, and thus the cell can be easily separated.
  • the valve 31 of the stopper portion 30 is operated to close the opening portion 21 of the container 20. Then the rotation speed of the rotator 10 is reduced to stop the centrifuge. During centrifuging, the fluid of the lowest layer in the container 20 is discharged outside to the receiving unit 15 of the rotator 10, and thus only a material having a high specific gravity, such as stem cells, remains in the container 20. Accordingly, there is no need to segregate the layers that are centrifuged according to specific gravity after the centrifuging, and thus a desired material can be easily obtained.
  • FIG. 5 is a side view illustrating a supporting structure of a container 20 according to another embodiment of the present invention.
  • FIG. 6 is a side view of a portion of a centrifuge, illustrating the container of FIG. 5 in which a portion of separated material is collected.
  • the centrifuge illustrated in FIG. 5 includes: a rotator 60 rotating around the rotation axis; a container 20 that is coupled to the rotator 60 at a predetermined angle ⁇ ; and a stopper portion 70 opening or closing an opening portion 21 of the container 20.
  • a receiving unit is formed in the rotator 10 to collect a centrifuged material.
  • a connecting portion 80 is connected to the stopper portion 70 to discharge the centrifuged material outside and collect the centrifuged material.
  • the connecting portion 80 connects the container 20 to the outside.
  • the connecting portion 80 may include at least one discharge pipe 81 discharging the material in the container 20 to the outside and at least one inflow pipe 82 injecting a fluid into the container 20 from the outside.
  • One discharge pipe 81 may be installed for separating only a lower fluid of the container 20, or a plurality of discharge pipes 81 may be installed to distinguish each layer to discharge the layers.
  • the connecting portion 80 is connected to the container 20 via the stopper portion
  • the stopper portion 70 may include a valve 71 having a function of opening or closing the connecting portion 80.
  • the valve 71 can open or close the connecting portion 80 by being controlled by a controller (not shown).
  • the valve 71 may be connected to the controller (not shown) via a signal line 72 or wirelessly.
  • the discharge pipe 81 is connected to a collection tank (not shown) that is installed in the centrifuge and may help the material flowing down by gravity to be collected to the collection tank.
  • a pump (not shown) may also be used. That is, a pump may be connected to the discharge pipe 81 and a negative pressure (less than atmospheric pressure) produced by the pump is used to discharge the material in the container 20 through the discharge pipe 81 to the collection tank.
  • the inflow pipe 82 injects a fluid into the container 20.
  • a negative pressure is formed inside the container 20.
  • a phenomenon such as reflux may occur. Such phenomenon can be prevented by injecting a fluid such as a dilution solution.
  • the connecting portion 80 can be connected to the outside of the container 20 through a rotation coupling (not shown).
  • the rotation coupling (not shown) connects the connecting portion 80 to the outside of the container 20 and at the same time is rotated by twisting elasticity of the connecting portion 80, thus preventing the connecting portion 80 from being twisted according to rotation of the rotator 60.
  • a material (sample) to be centrifuged is delivered into the container 20 and the container 20 is coupled to the rotator 60.
  • the container 20 is coupled to the rotator 60 by the stopper portion 70 and thus an axis P in a length direction of the container 20 is maintained at a predetermined angle ⁇ with respect to a rotation axis O of the rotator 60.
  • the stopper portion 70 should close the opening portion 21 of the container 20 so that the material in the container 20 may not flow out to the outside.
  • the container 60 rotates around the rotation axis O. Since the container 20 is coupled to the rotator 60, the container 20 is rotated together with the rotator 60. As a centrifugal force is exerted on the material injected into the container 20, the material in the container 20 is centrifuged according to specific gravity. While the rotator 60 is rotated around the rotation axis O, the axis P in a length direction of the container 20 is maintained at a predetermined angle ⁇ with respect to the rotation axis O.
  • an outermost fluid layer 91 formed of a material having a high specific gravity and high fluid resistance such as stem cells is formed relatively far away from the rotation axis O.
  • Inner fluid layers 92 formed of a material having a low specific gravity and low fluid resistance are formed relatively near the rotation axis O.
  • the outermost fluid layer 91 including the stem cells is pressurized and adhered to a wall surface of an end of the container 20 due to the adhesive force of the stem cells themselves and the centrifugal force.
  • the valve 71 of the stopper portion 70 is operated to close the connecting portion 80 of the container 20. Then the rotation speed of the rotator 60 is reduced to stop the centrifuge. During centrifuging, the inner fluid layers 92 in the container 20 are discharged outside, and thus only a material having a high specific gravity, such as stem cells, remains in the container 20. Accordingly, there is no need to segregate the layers that are centrifuged according to specific gravity after the centrifuging, and thus a desired material can be easily obtained.
  • FlG. 7 is a flowchart illustrating a centrifugal method according to an embodiment of the present invention.
  • the centrifuging method includes: delivering a material into the container (SlOO); detachably coupling the container to the rotator such that an opening portion of the container is maintained at a predetermined angle with respect to a rotation axis of the rotator (Sl 10); centrifuging the material in the container by rotating the rotator (S 120); discharging some of the layers of the centrifuged material to the outside of the container by opening the stopper portion coupled to the opening portion while the rotator (S 130) is rotating; closing the stopper portion (S 140); stopping the rotation of the rotator (S 150); and collecting the centrifuged material by separating the container from the rotator (S 160).
  • the stopper portion is opened while the rotator is continuously being rotated to discharge some of the layers of the material, and the rotation speed of the rotator can be reduced to a second speed that is lower than a first speed in operation S 120.
  • the second speed may be set such that some layers that are positioned on the outer edge of the container are remained in the container and the rest of the layers can be discharged through the opening portion of the container by gravity.
  • a discharge pipe connected to the container may be opened to discharge some layers to the outside. Then a pump may be connected to the discharge pipe, and the material can be discharged to the outside by pressure produced by the pump. Also, in operation S 130, an inflow pipe connected to the container may be opened by the stopper portion to inject an external fluid such as a dilution solution into the container to keep the pressure of the container constant.
  • the rotation speed of the rotator can be reduced to a second speed in operation
  • the present invention relates to a centrifuge and a centrifuging method, and more particularly, to a centrifuge and a centrifuging method in which, when centrifuging is performed by rotation of a rotator, a layer of a material having low fluid resistance and low specific gravity and a layer having high fluid resistance and high specific gravity among the layers separated by centrifuging can be classified and collected accurately and easily by opening an opening portion of a container.
  • the centrifuge and the centrifuging method according to the present invention can be applied to all kinds of materials that can move downward along an inclined surface, that is, both to liquids and solids such as liquid, powder, gel, a mixture of liquid and solid, colloids, and solids that are nearly spherical.

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  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Analytical Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Hematology (AREA)
  • Clinical Laboratory Science (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Centrifugal Separators (AREA)

Abstract

La présente invention concerne une centrifugeuse et un procédé de centrifugation dans lesquels les couches d'un matériau centrifugé peuvent se classifier et se recueillir avec précision et facilement, sans traitement de séparation ou de lavage, par ouverture ou fermeture d'une partie ouvrante d'un récipient dans un traitement de centrifugation. Cette centrifugeuse comprend un rotor, un récipient et un clapet. Le récipient qui est couplé au rotor et dans lequel on injecte le matériau, comporte une partie ouvrante, inclinée selon un angle défini par rapport à un axe de rotation du rotor de façon que la partie ouvrante soit dirigée vers le bas. Le clapet vient fermer la partie ouvrante. Lorsque la vitesse du rotor atteint une vitesse prédéterminée, centrifugeant ainsi le matériau, le clapet ouvre la partie ouvrante de façon que le matériau contenu dans le récipient s'écoule vers l'extérieur.
PCT/KR2007/000980 2006-06-30 2007-02-26 Centrifugeuse et procédé de centrifugation Ceased WO2008002004A1 (fr)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
KR10-2006-0060515 2006-06-30
KR20060060515 2006-06-30
KR10-2006-0111265 2006-11-10
KR1020060111265A KR100772970B1 (ko) 2006-06-30 2006-11-10 원심분리기 및 원심분리방법

Publications (1)

Publication Number Publication Date
WO2008002004A1 true WO2008002004A1 (fr) 2008-01-03

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PCT/KR2007/000980 Ceased WO2008002004A1 (fr) 2006-06-30 2007-02-26 Centrifugeuse et procédé de centrifugation

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US20100069215A1 (en) * 2006-06-30 2010-03-18 Medikan Inc. Centrifuge and centrifuging method

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US6273849B1 (en) * 1998-01-30 2001-08-14 Fresenius Ag Centrifuge and line for supplying and/or removing at least one fluid from the separation unit of a centrifuge to a stationary connection
JP2005013783A (ja) * 2003-06-23 2005-01-20 Moriai Seiki Kk 液体遠心分離装置
US20050274679A1 (en) * 2004-06-09 2005-12-15 Kao Yuan-Shiang Device and method for preparing washed red blood cells for newborn transfusions

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US5422018A (en) * 1994-01-31 1995-06-06 Applied Imaging Centrifuge tube and adaptor
US6273849B1 (en) * 1998-01-30 2001-08-14 Fresenius Ag Centrifuge and line for supplying and/or removing at least one fluid from the separation unit of a centrifuge to a stationary connection
JP2005013783A (ja) * 2003-06-23 2005-01-20 Moriai Seiki Kk 液体遠心分離装置
US20050274679A1 (en) * 2004-06-09 2005-12-15 Kao Yuan-Shiang Device and method for preparing washed red blood cells for newborn transfusions

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20100069215A1 (en) * 2006-06-30 2010-03-18 Medikan Inc. Centrifuge and centrifuging method
US8298128B2 (en) * 2006-06-30 2012-10-30 Medikan Inc. Centrifuge separating fluids by adjusting rotation speed using rotator and centrifuging method of the same

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