WO2008096562A1 - Microchip inspection system, microchip, microchip inspection device and program - Google Patents
Microchip inspection system, microchip, microchip inspection device and program Download PDFInfo
- Publication number
- WO2008096562A1 WO2008096562A1 PCT/JP2008/050115 JP2008050115W WO2008096562A1 WO 2008096562 A1 WO2008096562 A1 WO 2008096562A1 JP 2008050115 W JP2008050115 W JP 2008050115W WO 2008096562 A1 WO2008096562 A1 WO 2008096562A1
- Authority
- WO
- WIPO (PCT)
- Prior art keywords
- microchip
- target substance
- reaction
- fluorescence intensity
- section
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Ceased
Links
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/645—Specially adapted constructive features of fluorimeters
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/75—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated
- G01N21/77—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator
- G01N2021/7769—Measurement method of reaction-produced change in sensor
- G01N2021/7786—Fluorescence
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
- G01N35/00029—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor provided with flat sample substrates, e.g. slides
- G01N2035/00099—Characterised by type of test elements
- G01N2035/00158—Elements containing microarrays, i.e. "biochip"
Landscapes
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Immunology (AREA)
- Physics & Mathematics (AREA)
- General Physics & Mathematics (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Pathology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Optics & Photonics (AREA)
- Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
- Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
- Automatic Analysis And Handling Materials Therefor (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
It is intended to provide a microchip inspection system whereby a change in fluorescence intensity before and after a reaction can be exactly measured and thus detection can be conducted at a high accuracy. This object is achieved by using a microchip inspection system comprising: using a microchip having a prereaction measurement section in which the fluorescence intensity is detected before reacting a target substance with a fluorescence labeled reagent binding specifically to the target substance, a reaction section in which the target substance is reacted with the above-described reagent, and a postreaction measurement section which is located at a position different from the reaction section and in which the fluorescence intensity is detected after reacting the target substance with a the above-described reagent; detecting the fluorescence intensity before the reaction in the prereaction measurement section; reacting the target substance with the above-described reagent in the reaction section; then transferring the reaction mixture comprising the target substance and the above-described reagent from the reaction section into the postreaction measurement section; and detecting the fluorescence intensity after reacting the target substance with the above-described reagent in the postreaction measurement section.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP2008557035A JPWO2008096562A1 (en) | 2007-02-06 | 2008-01-09 | Microchip inspection system, microchip, microchip inspection apparatus and program |
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP2007026543 | 2007-02-06 | ||
| JP2007-026543 | 2007-02-06 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| WO2008096562A1 true WO2008096562A1 (en) | 2008-08-14 |
Family
ID=39681473
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| PCT/JP2008/050115 Ceased WO2008096562A1 (en) | 2007-02-06 | 2008-01-09 | Microchip inspection system, microchip, microchip inspection device and program |
Country Status (2)
| Country | Link |
|---|---|
| JP (1) | JPWO2008096562A1 (en) |
| WO (1) | WO2008096562A1 (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2011022031A (en) * | 2009-07-16 | 2011-02-03 | Canon Inc | Reaction treatment apparatus and reaction treatment method |
| JP2015516583A (en) * | 2012-05-15 | 2015-06-11 | ウェルスタット ダイアグノスティクス，エルエルシー | Clinical diagnostic system including instrument and cartridge |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH0870876A (en) * | 1994-09-01 | 1996-03-19 | F Hoffmann La Roche Ag | Method for degrading and detecting oligonucleotide labeled with luminescent label |
| JP2002507434A (en) * | 1998-03-24 | 2002-03-12 | ボストン プロビス、インコーポレイテッド | Methods, kits and compositions for detection complexes |
| JP2006284323A (en) * | 2005-03-31 | 2006-10-19 | Konica Minolta Medical & Graphic Inc | Micro total analysis system |
-
2008
- 2008-01-09 WO PCT/JP2008/050115 patent/WO2008096562A1/en not_active Ceased
- 2008-01-09 JP JP2008557035A patent/JPWO2008096562A1/en not_active Withdrawn
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH0870876A (en) * | 1994-09-01 | 1996-03-19 | F Hoffmann La Roche Ag | Method for degrading and detecting oligonucleotide labeled with luminescent label |
| JP2002507434A (en) * | 1998-03-24 | 2002-03-12 | ボストン プロビス、インコーポレイテッド | Methods, kits and compositions for detection complexes |
| JP2006284323A (en) * | 2005-03-31 | 2006-10-19 | Konica Minolta Medical & Graphic Inc | Micro total analysis system |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2011022031A (en) * | 2009-07-16 | 2011-02-03 | Canon Inc | Reaction treatment apparatus and reaction treatment method |
| JP2015516583A (en) * | 2012-05-15 | 2015-06-11 | ウェルスタット ダイアグノスティクス，エルエルシー | Clinical diagnostic system including instrument and cartridge |
Also Published As
| Publication number | Publication date |
|---|---|
| JPWO2008096562A1 (en) | 2010-05-20 |
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