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WO2008090826A1 - 間葉系細胞の製造方法、歯の製造方法及び歯形成用間葉系細胞 - Google Patents

間葉系細胞の製造方法、歯の製造方法及び歯形成用間葉系細胞 Download PDF

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WO2008090826A1
WO2008090826A1 PCT/JP2008/050641 JP2008050641W WO2008090826A1 WO 2008090826 A1 WO2008090826 A1 WO 2008090826A1 JP 2008050641 W JP2008050641 W JP 2008050641W WO 2008090826 A1 WO2008090826 A1 WO 2008090826A1
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cell
tooth
positive
mesenchymal cell
mesenchymal
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Ceased
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PCT/JP2008/050641
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English (en)
French (fr)
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Takashi Tsuji
Ritsuko Morita
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Otsuka Chemical Co Ltd
Tokyo University of Science
Organ Technologies Inc
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Otsuka Chemical Co Ltd
Tokyo University of Science
Organ Technologies Inc
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Priority to US12/523,918 priority Critical patent/US8574904B2/en
Priority to CA002676174A priority patent/CA2676174A1/en
Priority to KR1020097017305A priority patent/KR20090109564A/ko
Priority to AU2008208433A priority patent/AU2008208433B2/en
Priority to EP08703491A priority patent/EP2119768A4/en
Priority to CN2008800028601A priority patent/CN101842477B/zh
Publication of WO2008090826A1 publication Critical patent/WO2008090826A1/ja
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

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    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0652—Cells of skeletal and connective tissues; Mesenchyme
    • C—CHEMISTRY; METALLURGY
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    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0652—Cells of skeletal and connective tissues; Mesenchyme
    • C12N5/0654—Osteocytes, Osteoblasts, Odontocytes; Bones, Teeth
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
    • A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
    • A61K35/32—Bones; Osteocytes; Osteoblasts; Tendons; Tenocytes; Teeth; Odontoblasts; Cartilage; Chondrocytes; Synovial membrane
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K6/00—Preparations for dentistry
    • A61K6/80—Preparations for artificial teeth, for filling teeth or for capping teeth
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P1/00—Drugs for disorders of the alimentary tract or the digestive system
    • A61P1/02—Stomatological preparations, e.g. drugs for caries, aphtae, periodontitis
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
    • A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/30—Organic components
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    • C12N2500/00—Specific components of cell culture medium
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    • C12N2500/62—DMSO
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/30—Hormones
    • C12N2501/38—Hormones with nuclear receptors
    • C12N2501/385—Hormones with nuclear receptors of the family of the retinoic acid recptor, e.g. RAR, RXR; Peroxisome proliferator-activated receptor [PPAR]
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    • C12N2506/00—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells
    • C12N2506/02—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells from embryonic cells

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  • Health & Medical Sciences (AREA)
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Abstract

　本発明は、歯を形成するために用いられる間葉系細胞を製造する間葉系細胞の製造方法であって、全能性幹細胞を分化誘導剤の存在下で培養して、ＣＤ４４陽性且つＣＤ２９陽性細胞又はＣＤ４４陽性且つＣＤ１０６陽性細胞を含む分化誘導処理後の細胞集団を生成すること、前記分化誘導処理後の細胞集団から、ＣＤ４４陽性且つＣＤ２９陽性細胞又はＣＤ４４陽性且つＣＤ１０６陽性細胞を、歯形成用間葉系細胞として選別することを含む間葉系細胞の製造方法を提供する。また、間葉系細胞と上皮系細胞とのいずれか一方から実質的になる第１の細胞集合体と、いずれか他方のみから実質的になる第２の細胞集合体とを、細胞の接触状態を保持可能な支持担体の内部に混合することなく密着させて配置して培養することを含み、上記間葉系細胞を、上記歯形成用間葉系細胞を含むものとする歯の製造方法を提供する。
PCT/JP2008/050641 2007-01-22 2008-01-18 間葉系細胞の製造方法、歯の製造方法及び歯形成用間葉系細胞 Ceased WO2008090826A1 (ja)

Priority Applications (6)

Application Number Priority Date Filing Date Title
US12/523,918 US8574904B2 (en) 2007-01-22 2008-01-18 Method for production of mesenchymal cell, method for production of tooth, and mesenchymal cell for formation of tooth
CA002676174A CA2676174A1 (en) 2007-01-22 2008-01-18 Method for production of mesenchymal cell, method for production of tooth, and mesenchymal cell for formation of tooth
KR1020097017305A KR20090109564A (ko) 2007-01-22 2008-01-18 간엽계 세포의 제조방법, 이빨의 제조방법 및 이빨 형성용 간엽계 세포
AU2008208433A AU2008208433B2 (en) 2007-01-22 2008-01-18 Method for production of mesenchymal cell, method for production of tooth, and mesenchymal cell for formation of tooth
EP08703491A EP2119768A4 (en) 2007-01-22 2008-01-18 METHOD FOR MANUFACTURING MESENCHYMAL CELL, METHOD FOR MANUFACTURING TOOTH AND MESENCHYMAL CELL FOR TOOTH FORMATION
CN2008800028601A CN101842477B (zh) 2007-01-22 2008-01-18 间充质细胞的制造方法、牙的制造方法及牙形成用间充质细胞

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
JP2007011805 2007-01-22
JP2007-011805 2007-01-22

Publications (1)

Publication Number Publication Date
WO2008090826A1 true WO2008090826A1 (ja) 2008-07-31

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PCT/JP2008/050641 Ceased WO2008090826A1 (ja) 2007-01-22 2008-01-18 間葉系細胞の製造方法、歯の製造方法及び歯形成用間葉系細胞

Country Status (10)

Country Link
US (1) US8574904B2 (ja)
EP (1) EP2119768A4 (ja)
JP (1) JP5069572B2 (ja)
KR (1) KR20090109564A (ja)
CN (1) CN101842477B (ja)
AU (1) AU2008208433B2 (ja)
CA (1) CA2676174A1 (ja)
RU (1) RU2465325C2 (ja)
TW (1) TW200844234A (ja)
WO (1) WO2008090826A1 (ja)

Cited By (2)

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WO2012108069A1 (ja) * 2011-02-09 2012-08-16 株式会社オーガンテクノロジーズ ガイドを有する移植用再生器官原基の製造方法、当該方法によって製造される、ガイドを有する移植用再生器官原基を含む組成物、およびガイドを有する移植用再生器官原基の移植方法
CN115992215A (zh) * 2022-09-22 2023-04-21 中南大学 非综合征型先天缺牙基因突变位点及其应用

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US8927284B2 (en) 2009-01-28 2015-01-06 Organ Technologies, Inc. Method for producing tooth
SG184330A1 (en) * 2010-04-07 2012-10-30 Organ Technologies Inc Method for restoring alveolar bone via transplantation of a regenerated tooth unit
US20130089827A1 (en) 2010-04-07 2013-04-11 Organ Technologies, Inc. Methods For Manufacturing A Regenerated Tooth Unit
SG192924A1 (en) 2011-02-24 2013-09-30 Organ Technologies Inc Method of preparing regenerated hair follicle germ for transplantation in which hair color is controlled, composition including regenerated hair follicle germ for transplantation, and method of transplanting regenerated hair follicle germ
US10022205B2 (en) 2012-02-01 2018-07-17 Organ Technologies, Inc. Dental implant and method for producing same
EP2633870B1 (en) * 2012-02-29 2018-08-01 Technische Universität Berlin Method of preparing an artificial tooth primordium in vitro and artificial tooth primordium derived therefrom
US9782443B2 (en) * 2013-02-05 2017-10-10 Guangzhou Institutes Of Biomedicine And Health, Chinese Academy Of Sciences Preparing tooth-like structure using stem cell
CN107002035A (zh) * 2014-08-14 2017-08-01 阿威他国际有限公司 干细胞组合物和生产用于治疗应用的干细胞的方法
CN104450621B (zh) * 2014-09-30 2018-10-19 首都医科大学附属北京口腔医院 Wdr63基因在间充质干细胞骨向和牙向分化过程中的调控方法
JP6632800B2 (ja) * 2014-12-24 2020-01-22 メディア株式会社 歯周病診断支援装置、歯周病診断支援システム、歯周病診断支援プログラム及び歯周病診断支援方法
CN105866432B (zh) * 2016-05-11 2018-01-26 天津欣普赛尔生物医药科技有限公司 用于鉴定牙髓间充质干细胞的试剂盒
JP7094567B2 (ja) * 2017-04-27 2022-07-04 国立大学法人京都大学 神経堤細胞および交感神経細胞の製造方法
WO2019235375A1 (ja) 2018-06-04 2019-12-12 株式会社オーガンテクノロジーズ 再生毛包原基の製造方法
CN111197027A (zh) * 2018-11-16 2020-05-26 中国科学院广州生物医药与健康研究院 分离牙胚单细胞的方法及应用
RU2716485C1 (ru) * 2019-02-15 2020-03-11 федеральное государственное бюджетное образовательное учреждение высшего образования "Московский государственный медико-стоматологический университет имени А.И. Евдокимова" Министерства здравоохранения Российской Федерации (ФГБОУ ВО МГМСУ им. А.И. Евдокимова Минздрава России) Способ выращивания эмали в эксперименте
CN111407755A (zh) * 2020-04-30 2020-07-14 重庆医科大学附属口腔医院 视黄酸在牙齿发育中的应用
CN113755423B (zh) * 2021-10-12 2024-04-26 徐海 胚胎癌性细胞在制备3d细胞培养基质中的应用、制备方法及3d细胞培养基质
WO2024058511A1 (ko) * 2022-09-14 2024-03-21 연세대학교 산학협력단 줄기세포의 경조직으로의 분화 방법

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Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2012108069A1 (ja) * 2011-02-09 2012-08-16 株式会社オーガンテクノロジーズ ガイドを有する移植用再生器官原基の製造方法、当該方法によって製造される、ガイドを有する移植用再生器官原基を含む組成物、およびガイドを有する移植用再生器官原基の移植方法
JP5932671B2 (ja) * 2011-02-09 2016-06-08 株式会社オーガンテクノロジーズ ガイドを有する移植用再生器官原基の製造方法、当該方法によって製造される、ガイドを有する移植用再生器官原基を含む組成物、およびガイドを有する移植用再生器官原基の移植方法
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CN115992215A (zh) * 2022-09-22 2023-04-21 中南大学 非综合征型先天缺牙基因突变位点及其应用

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JP2008200033A (ja) 2008-09-04
US20100119997A1 (en) 2010-05-13
RU2009130630A (ru) 2011-02-27
CA2676174A1 (en) 2008-07-31
EP2119768A1 (en) 2009-11-18
EP2119768A4 (en) 2010-06-09
JP5069572B2 (ja) 2012-11-07
CN101842477B (zh) 2013-03-27
TW200844234A (en) 2008-11-16
AU2008208433B2 (en) 2014-04-03
KR20090109564A (ko) 2009-10-20
US8574904B2 (en) 2013-11-05
RU2465325C2 (ru) 2012-10-27
CN101842477A (zh) 2010-09-22
AU2008208433A1 (en) 2008-07-31

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