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WO2008084566A1 - モデルマウス、その製造方法およびその用途 - Google Patents

モデルマウス、その製造方法およびその用途 Download PDF

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Publication number
WO2008084566A1
WO2008084566A1 PCT/JP2007/063756 JP2007063756W WO2008084566A1 WO 2008084566 A1 WO2008084566 A1 WO 2008084566A1 JP 2007063756 W JP2007063756 W JP 2007063756W WO 2008084566 A1 WO2008084566 A1 WO 2008084566A1
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Prior art keywords
gsdmd
mouse
cells
gene
model mouse
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Ceased
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PCT/JP2007/063756
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English (en)
French (fr)
Inventor
Toshihiko Shiroishi
Masaru Tamura
Tomoaki Fujii
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Research Organization of Information and Systems
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Research Organization of Information and Systems
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Priority to JP2008552997A priority Critical patent/JPWO2008084566A1/ja
Publication of WO2008084566A1 publication Critical patent/WO2008084566A1/ja
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

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    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • G01N33/5008—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
    • G01N33/5044—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics involving specific cell types
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
    • A01K67/027—New or modified breeds of vertebrates
    • A01K67/0275—Genetically modified vertebrates, e.g. transgenic
    • A01K67/0276—Knock-out vertebrates
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/46—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates
    • C07K14/47—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals
    • C07K14/4701—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals not used
    • C07K14/4702—Regulators; Modulating activity
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • G01N33/5008—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
    • G01N33/5082—Supracellular entities, e.g. tissue, organisms
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2217/00—Genetically modified animals
    • A01K2217/07—Animals genetically altered by homologous recombination
    • A01K2217/075—Animals genetically altered by homologous recombination inducing loss of function, i.e. knock out
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2227/00—Animals characterised by species
    • A01K2227/10—Mammal
    • A01K2227/105—Murine
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2267/00—Animals characterised by purpose
    • A01K2267/03—Animal model, e.g. for test or diseases

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Immunology (AREA)
  • Molecular Biology (AREA)
  • Cell Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Urology & Nephrology (AREA)
  • Hematology (AREA)
  • Toxicology (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • Medicinal Chemistry (AREA)
  • Analytical Chemistry (AREA)
  • Food Science & Technology (AREA)
  • Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Zoology (AREA)
  • Environmental Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biophysics (AREA)
  • Veterinary Medicine (AREA)
  • Animal Behavior & Ethology (AREA)
  • Animal Husbandry (AREA)
  • Biodiversity & Conservation Biology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

　腸の上皮細胞におけるＧｓｄｍｄおよびそれをコードするＧｓｄｍｄ遺伝子の機能を解明するための、モデルマウスを提供する。染色体上でＧｓｄｍｄをコードするＧｓｄｍｄ遺伝子がノックアウトされたホモ接合体を有し、Ｇｓｄｍｄが非発現であるマウスを、モデルマウスとする。まず、マウスゲノムＤＮＡにおけるＧｓｄｍｄ遺伝子のエキソン２を含む断片を有するターゲティングベクターをマウス胚幹細胞に導入し、相同組換えによりＧｓｄｍｄ遺伝子がノックアウトされた胚幹細胞からキメラ胚細胞を作製する。これを仮親マウスに移植して、生まれたＧｓｄｍｄ遺伝子がノックアウトされたキメラマウスを交配して、ヘテロ接合体マウスを選択し、前記へテロ接合体マウス同士を交配することによって、Ｇｓｄｍｄ遺伝子がノックアウトされたホモ接合体のモデルマウスが得られる。
PCT/JP2007/063756 2007-01-11 2007-07-10 モデルマウス、その製造方法およびその用途 Ceased WO2008084566A1 (ja)

Priority Applications (1)

Application Number Priority Date Filing Date Title
JP2008552997A JPWO2008084566A1 (ja) 2007-01-11 2007-07-10 モデルマウス、その製造方法およびその用途

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
JP2007-003398 2007-01-11
JP2007003398 2007-01-11

Publications (1)

Publication Number Publication Date
WO2008084566A1 true WO2008084566A1 (ja) 2008-07-17

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PCT/JP2007/063756 Ceased WO2008084566A1 (ja) 2007-01-11 2007-07-10 モデルマウス、その製造方法およびその用途

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JP (1) JPWO2008084566A1 (ja)
WO (1) WO2008084566A1 (ja)

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2018214823A1 (zh) * 2017-05-25 2018-11-29 朱献军 胰岛β细胞条件性敲除Tmem30a基因小鼠模型的构建方法及应用
CN114657214A (zh) * 2022-02-25 2022-06-24 陕西中医药大学 一种自发性肾阴虚Ptgds基因敲除大鼠模型的构建方法
CN115725687A (zh) * 2022-10-08 2023-03-03 浙江大学 猪源抗菌短肽的筛选法
CN116369278A (zh) * 2023-05-17 2023-07-04 上海溯源生物技术有限公司 一种幽门螺杆菌感染的动物模型构建方法

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2004076666A1 (ja) * 2003-02-28 2004-09-10 Toshihiko Shiroishi ガスダーミンファミリー
WO2006019153A1 (ja) * 2004-08-20 2006-02-23 Inter University Research Institution Corporation, Research Organization Of Information And Systems GsdmA遺伝子の機能が抑制され、癌化関連遺伝子の機能が促進または抑制された非ヒト動物

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2004076666A1 (ja) * 2003-02-28 2004-09-10 Toshihiko Shiroishi ガスダーミンファミリー
WO2006019153A1 (ja) * 2004-08-20 2006-02-23 Inter University Research Institution Corporation, Research Organization Of Information And Systems GsdmA遺伝子の機能が抑制され、癌化関連遺伝子の機能が促進または抑制された非ヒト動物

Non-Patent Citations (10)

* Cited by examiner, † Cited by third party
Title
FUJII T. ET AL.: "Expression and functional analyses of GasderminD(GsdmD)", GENES & GENETIC SYSTEMS, vol. 80, no. 6, 2005, pages 462, XP003022943 *
FUJII T. ET AL.: "Gasdermin (Gsdm) is involved in maintaining of homeostasis of intestinal epithelial ceels in intestinal tract", GENES & GENETIC SYSTEMS, vol. 81, no. 6, December 2006 (2006-12-01), pages 450, XP003022935 *
FUJII T. ET AL.: "Gasdermin(Gsdm)family Idenshi no Tanri to sono Hatsugen Kaiseki", JAPAN SOCIETY OF DEVELOPMENT BIOLOGISTS TAIKAI HAPPYO YOSHISHU, vol. 36TH, 2003, pages 55, XP002996880 *
FUJII T. ET AL.: "Shokakan ni Oite, Gasdermin (Gsdm) wa Johi Saibo no Kojosei Iji ni Kanyo suru", 78TH ANNUAL MEETING OF THE GENETICS SOECIETY OF JAPAN, PROGRAMS & ABSTRACTS, 1 September 2006 (2006-09-01), pages 164, 2E08, XP003022936 *
FUJII T. ET AL.: "Shokakan no Oite, Gasdermin (Gsdm) wa Johi Saibo no Kojosei Iji ni Kanyo suru", THE GENETICS SOCIETY OF JAPAN, 26 September 2006 (2006-09-26), XP003022942, Retrieved from the Internet <URL:http://www.brc.riken.jp/iden78/program2.pdf> *
FUJII T.: "Expression profiling and functional analysis of Gasdermin (Gsdm) family genes in mouse intestinal tract", 28 August 2006 (2006-08-28), XP003022937, Retrieved from the Internet <URL:http://www.nig.ac.jp/cgi-bin/seminar.pl?action=display&target=20060828005_&lang=Japanese> *
KATOH M. ET AL.: "Identification and characterization of human DFNA5L, mouse Dfna5l, and rat Dfna5l genes in silico", INTERNATIONAL JOURNAL OF ONCOLOGY, vol. 25, no. 3, 2004, pages 765 - 770, XP003022938 *
SUN Q. ET AL.: "Progress of researches on gene function of GSDMDC family", YI CHUAN, vol. 28, no. 5, May 2006 (2006-05-01), pages 596 - 600, XP003022939 *
TAMURA M. ET AL.: "Gasdermin(Gsdm/GSDM) family no Hatsugen to Shinkateki Sokumen ni Tsuite", JAPAN SOCIETY OF DEVELOPMENT BIOLOGISTS TAIKAI HAPPYO YOSHISHU, vol. 39TH, 2006, pages 174, XP003022940 *
TAMURA M. ET AL.: "Shinki Gsdm family Isenshi no Tanri to sono Hatsugen Kaiseki", JAPAN SOCIETY OF DEVELOPMENT BIOLOGISTS TAIKAI HAPPYO YOSHISHU, vol. 38TH, 2005, pages 243, XP003022941 *

Cited By (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2018214823A1 (zh) * 2017-05-25 2018-11-29 朱献军 胰岛β细胞条件性敲除Tmem30a基因小鼠模型的构建方法及应用
CN114657214A (zh) * 2022-02-25 2022-06-24 陕西中医药大学 一种自发性肾阴虚Ptgds基因敲除大鼠模型的构建方法
CN114657214B (zh) * 2022-02-25 2024-04-16 陕西中医药大学 一种自发性肾阴虚Ptgds基因敲除大鼠模型的构建方法
CN115725687A (zh) * 2022-10-08 2023-03-03 浙江大学 猪源抗菌短肽的筛选法
CN115725687B (zh) * 2022-10-08 2023-12-12 浙江大学 猪源抗菌短肽的筛选法
CN116369278A (zh) * 2023-05-17 2023-07-04 上海溯源生物技术有限公司 一种幽门螺杆菌感染的动物模型构建方法

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