[go: up one dir, main page]

WO2007147064A3 - Modified soy proteins and methods of use - Google Patents

Modified soy proteins and methods of use Download PDF

Info

Publication number
WO2007147064A3
WO2007147064A3 PCT/US2007/071235 US2007071235W WO2007147064A3 WO 2007147064 A3 WO2007147064 A3 WO 2007147064A3 US 2007071235 W US2007071235 W US 2007071235W WO 2007147064 A3 WO2007147064 A3 WO 2007147064A3
Authority
WO
WIPO (PCT)
Prior art keywords
calcium
proteins
modified
binding
protein
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/US2007/071235
Other languages
French (fr)
Other versions
WO2007147064A2 (en
Inventor
Jian G Dong
Shirong Zhang
A Gururaj Rao
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Pioneer Hi Bred International Inc
Original Assignee
Pioneer Hi Bred International Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Pioneer Hi Bred International Inc filed Critical Pioneer Hi Bred International Inc
Publication of WO2007147064A2 publication Critical patent/WO2007147064A2/en
Publication of WO2007147064A3 publication Critical patent/WO2007147064A3/en
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/415—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from plants
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23J—PROTEIN COMPOSITIONS FOR FOODSTUFFS; WORKING-UP PROTEINS FOR FOODSTUFFS; PHOSPHATIDE COMPOSITIONS FOR FOODSTUFFS
    • A23J3/00—Working-up of proteins for foodstuffs
    • A23J3/14—Vegetable proteins
    • A23J3/16—Vegetable proteins from soybean
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES, NOT OTHERWISE PROVIDED FOR; PREPARATION OR TREATMENT THEREOF
    • A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
    • A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
    • A23L33/16—Inorganic salts, minerals or trace elements
    • A23L33/165—Complexes or chelates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • C12N15/8251—Amino acid content, e.g. synthetic storage proteins, altering amino acid biosynthesis
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Organic Chemistry (AREA)
  • Molecular Biology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Nutrition Science (AREA)
  • General Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Cell Biology (AREA)
  • Physics & Mathematics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Food Science & Technology (AREA)
  • Plant Pathology (AREA)
  • Polymers & Plastics (AREA)
  • Botany (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Medicinal Chemistry (AREA)
  • Inorganic Chemistry (AREA)
  • Mycology (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Coloring Foods And Improving Nutritive Qualities (AREA)

Abstract

Compositions and methods for preparing modified proteins are provided. In one embodiment, calcium-modified proteins are provided by operably linking a calcium-binding extension region capable of binding calcium to a protein of interest. The link of the extension can additionally cause increased solubility of the resulting protein and facilitate its formation of translucent gels. In this manner calcium enrich proteins can be obtained for use as food and beverage additives. Nucleotide and amino acid sequences for calcium-binding extension regions are provided and can be used to modify proteins, including soy proteins to provide calcium fortified proteins. Commercial varieties of soybeans modified to express particular soy protein, particular β-conglycinin subunits are provided. The soy proteins can be modified to contain at least one calcium-binding extension region.
PCT/US2007/071235 2006-06-14 2007-06-14 Modified soy proteins and methods of use Ceased WO2007147064A2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US81353206P 2006-06-14 2006-06-14
US60/813,532 2006-06-14

Publications (2)

Publication Number Publication Date
WO2007147064A2 WO2007147064A2 (en) 2007-12-21
WO2007147064A3 true WO2007147064A3 (en) 2008-05-15

Family

ID=38832864

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2007/071235 Ceased WO2007147064A2 (en) 2006-06-14 2007-06-14 Modified soy proteins and methods of use

Country Status (1)

Country Link
WO (1) WO2007147064A2 (en)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
PT2264055E (en) 2009-06-17 2014-05-07 Indena Spa Cloning, yeast expression, purification and biological activity of the extension region of the soybean 7s globulin alfa' subunit involved in hep g2 cell cholesterol homeostasis
EP2264054A1 (en) * 2009-06-17 2010-12-22 Indena S.p.A. Cloning, yeast expression, purification and biological activity of a truncated form of the soybean 7S globulin alfa ' subunit involved in Hep G2 cell cholesterol homeostasis
CA3042857A1 (en) * 2016-11-16 2018-05-24 Cellectis Methods for altering amino acid content in plants through frameshift mutations
WO2019111043A1 (en) * 2017-12-08 2019-06-13 Industrias Nutrigrains, S.A.P.I. De C.V. Protein compositions of vegetable-origin and method for obtaining the same

Non-Patent Citations (5)

* Cited by examiner, † Cited by third party
Title
DATABASE UniProt [online] 1 December 2001 (2001-12-01), "Beta-conglycinin alpha subunit.", XP002472519, retrieved from EBI accession no. UNIPROT:Q94LX2 Database accession no. Q94LX2 *
DATABASE UniProt [online] 1 March 2001 (2001-03-01), "Alpha' subunit of beta-conglycinin (Fragment).", XP002472520, retrieved from EBI accession no. UNIPROT:Q9FZP9 Database accession no. Q9FZP9 *
MARUYAMA N ET AL: "The roles of the N-linked glycans and extension regions of soybean beta-conglycinin in folding, assembly and structural features", EUROPEAN JOURNAL OF BIOCHEMISTRY, BERLIN, DE, vol. 258, 1998, pages 854 - 862, XP003004727, ISSN: 0014-2956 *
RAO A G A ET AL: "BINDING OF CALCIUM MAGNESIUM II AND ZINC II BY 7S FRACTION OF SOYBEAN PROTEINS", JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, vol. 24, no. 3, 1976, pages 490 - 494, XP001538100, ISSN: 0021-8561 *
TANDANG MARY ROSE G ET AL: "Evaluation of the solubility and emulsifying property of soybean proglycinin and rapeseed procruciferin in relation to structure modified by protein engineering", JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, vol. 53, no. 22, November 2005 (2005-11-01), pages 8736 - 8744, XP009097163, ISSN: 0021-8561 *

Also Published As

Publication number Publication date
WO2007147064A2 (en) 2007-12-21

Similar Documents

Publication Publication Date Title
WO2008064224A3 (en) Use of structured plant protein products to produce emulsified meat products
WO2005120524A3 (en) Nutritional formulations
MX2013012844A (en) Amino acid sequences directed against il-17a, il-17f and/or il17-a/f and polypeptides comprising the same.
WO2012008994A3 (en) Food compositions having a realistic meat-like appearance and texture
WO2006034172A3 (en) GLYCININ-RICH AND β-CONGLYCININ-RICH VEGETABLE PROTEIN FRACTIONS
PL2225076T3 (en) Binder for materials based on wood chips and/or wood fibers, method for the production of said binder, and molded article
SI1888641T1 (en) Serum albumin binding proteins
HK1206370A1 (en) Charged nutritive proteins and methods
DK1929873T3 (en) Food material and food product using this
WO2009099641A3 (en) Stabilized protein compositions
HK1206764A1 (en) Nutritive proteins and methods
WO2007104529A3 (en) Amino acid sequences directed against il-6 and polypeptides comprising the same for the treatment of diseases and disorders associated with il-6-mediated signalling
EA201070870A1 (en) COMPOSITION, CONTAINING PROTEIN AND DISPERSED FAT
WO2009068627A3 (en) Amino acid sequences directed against heterodimeric cytokines and/or their receptors and polypeptides comprising the same
PH12012502272A1 (en) Biological materials related to her3
TW200505354A (en) Transglutaminase soy fish and meat products and analogs thereof
WO2010142551A8 (en) Amino acid sequences directed against receptors to cytokines belonging to the il-17 family
UA113863C2 (en) SUBSTANCE: PLANT-BASED MEAT mincemeat
MX2011007107A (en) Protein hydrolysate compositions.
WO2006132969A3 (en) Incorporation of non-naturally encoded amino acids into proteins
HK1206763A1 (en) Charged nutritive fragments, proteins and methods
BRPI0810149A2 (en) method for the production of a whey protein hydrolyzate, and, use of a whey protein hydrolyzate
WO2007022341A3 (en) Isolated soy protein having high molecular weight protein fractions and low molecular weight protein fractions
WO2007147064A3 (en) Modified soy proteins and methods of use
DK1890559T3 (en) Citrus fruit fibers in processed meat

Legal Events

Date Code Title Description
NENP Non-entry into the national phase

Ref country code: DE

NENP Non-entry into the national phase

Ref country code: RU

122 Ep: pct application non-entry in european phase

Ref document number: 07798574

Country of ref document: EP

Kind code of ref document: A2