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WO2007034169A3 - Cell selection and reporter system - Google Patents

Cell selection and reporter system Download PDF

Info

Publication number
WO2007034169A3
WO2007034169A3 PCT/GB2006/003484 GB2006003484W WO2007034169A3 WO 2007034169 A3 WO2007034169 A3 WO 2007034169A3 GB 2006003484 W GB2006003484 W GB 2006003484W WO 2007034169 A3 WO2007034169 A3 WO 2007034169A3
Authority
WO
WIPO (PCT)
Prior art keywords
cells
fusion
enzyme
cell selection
sorting
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/GB2006/003484
Other languages
French (fr)
Other versions
WO2007034169A2 (en
Inventor
Timothy Allsopp
Lillian Alexandra Hook
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Stem Cell Sciences UK Ltd
Original Assignee
Stem Cell Sciences UK Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Stem Cell Sciences UK Ltd filed Critical Stem Cell Sciences UK Ltd
Publication of WO2007034169A2 publication Critical patent/WO2007034169A2/en
Publication of WO2007034169A3 publication Critical patent/WO2007034169A3/en
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/62—DNA sequences coding for fusion proteins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2510/00—Genetically modified cells

Landscapes

  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Molecular Biology (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

A fusion protein has three domains, being an extracellular cell surface antigen or a fluorescent protein for sorting uses, an enzyme that enables staining of cells in tissues or tissue sections and an enzyme, such as antibiotic resistance, which protects cells from the effects of a toxic factor in culture medium. The fusion is used for purification and sorting of cells expressing the fusion under control of a cell or tissue-specific promoter and subsequent tracking and analyses of cells post transplantation.
PCT/GB2006/003484 2005-09-20 2006-09-20 Cell selection and reporter system Ceased WO2007034169A2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
GB0519197.8 2005-09-20
GB0519197A GB0519197D0 (en) 2005-09-20 2005-09-20 Cell selection and reporter system

Publications (2)

Publication Number Publication Date
WO2007034169A2 WO2007034169A2 (en) 2007-03-29
WO2007034169A3 true WO2007034169A3 (en) 2007-05-31

Family

ID=35249114

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/GB2006/003484 Ceased WO2007034169A2 (en) 2005-09-20 2006-09-20 Cell selection and reporter system

Country Status (3)

Country Link
GB (1) GB0519197D0 (en)
TW (1) TW200804598A (en)
WO (1) WO2007034169A2 (en)

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0731169A1 (en) * 1995-01-10 1996-09-11 University Of Edinburgh Vectors and use thereof for capturing target genes
WO2003014361A1 (en) * 2001-08-02 2003-02-20 Altana Pharma Ag Novel recombinant gene expression method by stop codon suppression

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0731169A1 (en) * 1995-01-10 1996-09-11 University Of Edinburgh Vectors and use thereof for capturing target genes
WO2003014361A1 (en) * 2001-08-02 2003-02-20 Altana Pharma Ag Novel recombinant gene expression method by stop codon suppression

Also Published As

Publication number Publication date
TW200804598A (en) 2008-01-16
GB0519197D0 (en) 2005-10-26
WO2007034169A2 (en) 2007-03-29

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Legal Events

Date Code Title Description
121 Ep: the epo has been informed by wipo that ep was designated in this application
DPE1 Request for preliminary examination filed after expiration of 19th month from priority date (pct application filed from 20040101)
NENP Non-entry into the national phase

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