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WO2007030373A3 - Method for in situ hybridization analysis - Google Patents

Method for in situ hybridization analysis Download PDF

Info

Publication number
WO2007030373A3
WO2007030373A3 PCT/US2006/034005 US2006034005W WO2007030373A3 WO 2007030373 A3 WO2007030373 A3 WO 2007030373A3 US 2006034005 W US2006034005 W US 2006034005W WO 2007030373 A3 WO2007030373 A3 WO 2007030373A3
Authority
WO
WIPO (PCT)
Prior art keywords
situ hybridization
hybridization analysis
temporal
ribonucleic acid
tissue sample
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/US2006/034005
Other languages
French (fr)
Other versions
WO2007030373A2 (en
Inventor
Thomas Curran
Dennis S Rice
Susan Magdaleno
Patricia Jensen
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
St Jude Childrens Research Hospital
Original Assignee
St Jude Childrens Research Hospital
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by St Jude Childrens Research Hospital filed Critical St Jude Childrens Research Hospital
Publication of WO2007030373A2 publication Critical patent/WO2007030373A2/en
Anticipated expiration legal-status Critical
Publication of WO2007030373A3 publication Critical patent/WO2007030373A3/en
Ceased legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/36—Embedding or analogous mounting of samples
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6841—In situ hybridisation
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00583—Features relative to the processes being carried out
    • B01J2219/00603—Making arrays on substantially continuous surfaces
    • B01J2219/00673—Slice arrays
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00718—Type of compounds synthesised
    • B01J2219/0072—Organic compounds
    • B01J2219/0074—Biological products
    • B01J2219/00743—Cells
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/30—Staining; Impregnating ; Fixation; Dehydration; Multistep processes for preparing samples of tissue, cell or nucleic acid material and the like for analysis
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/36—Embedding or analogous mounting of samples
    • G01N2001/368—Mounting multiple samples in one block, e.g. TMA [Tissue Microarrays]

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Analytical Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Immunology (AREA)
  • Biochemistry (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • General Physics & Mathematics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

The present invention is a method for in situ hybridization analysis of the temporal and/or spatial expression of one or more ribonucleic acid target sequences in a tissue sample. The disclosed method can be adapted for high- throughput analysis of a plurality of target sequences and a plurality of tissue samples and the method is highly sensitive based upon the combination of a radiolabeled ribonucleic acid probe and silver emulsif ication of the tissue sample to detect the temporal and/or spatial expression of the target sequence.
PCT/US2006/034005 2005-09-07 2006-08-30 Method for in situ hybridization analysis Ceased WO2007030373A2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US71494905P 2005-09-07 2005-09-07
US60/714,949 2005-09-07

Publications (2)

Publication Number Publication Date
WO2007030373A2 WO2007030373A2 (en) 2007-03-15
WO2007030373A3 true WO2007030373A3 (en) 2009-04-23

Family

ID=37836349

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2006/034005 Ceased WO2007030373A2 (en) 2005-09-07 2006-08-30 Method for in situ hybridization analysis

Country Status (1)

Country Link
WO (1) WO2007030373A2 (en)

Families Citing this family (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US10787701B2 (en) 2010-04-05 2020-09-29 Prognosys Biosciences, Inc. Spatially encoded biological assays
JP5893607B2 (en) 2010-04-05 2016-03-23 プログノシス バイオサイエンシズ インコーポレイテッドＰｒｏｇｎｏｓｙｓ Ｂｉｏｓｃｉｅｎｃｅｓ，Ｉｎｃ． Spatial-encoded biological assay
US20190300945A1 (en) 2010-04-05 2019-10-03 Prognosys Biosciences, Inc. Spatially Encoded Biological Assays
GB201106254D0 (en) * 2011-04-13 2011-05-25 Frisen Jonas Method and product
EP3901280B1 (en) 2012-10-17 2025-03-12 10x Genomics Sweden AB Methods and product for optimising localised or spatial detection of gene expression in a tissue sample
US9868979B2 (en) 2013-06-25 2018-01-16 Prognosys Biosciences, Inc. Spatially encoded biological assays using a microfluidic device
ES2994053T3 (en) 2015-04-10 2025-01-17 10X Genomics Sweden Ab Spatially distinguished, multiplex nucleic acid analysis of biological specimens
EP3532819B1 (en) 2016-10-31 2021-08-25 Agilent Technologies, Inc. Deparaffinization of tissue by electric field generation and ionization
US11525759B2 (en) 2018-04-24 2022-12-13 Agilent Technologies, Inc. Deparaffinization of tissue utilizing electric field
US11732299B2 (en) 2020-01-21 2023-08-22 10X Genomics, Inc. Spatial assays with perturbed cells
ES2989052T3 (en) 2020-05-22 2024-11-25 10X Genomics Inc Simultaneous spatiotemporal measurement of gene expression and cellular activity
US20230407379A1 (en) * 2020-09-30 2023-12-21 Agilent Technologies, Inc. Electric field aided sample preparation

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6696271B2 (en) * 2001-08-23 2004-02-24 The Regents Of The University Of California Frozen tissue microarray technology for analysis of RNA, DNA, and proteins

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6696271B2 (en) * 2001-08-23 2004-02-24 The Regents Of The University Of California Frozen tissue microarray technology for analysis of RNA, DNA, and proteins

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
BOURAS. T. ET AL.: "Overexpression of the Steroid Receptor Coactivator AIB1 in Breast Cancer Correlates with the Absence of Estrogen and Progesterone Receptors and Positivity for p53 and HER2/neu", CANCER RESEARCH, vol. 61, February 2001 (2001-02-01), pages 903 - 907 *
OLSEN. L.: "CVD: the intestinal crypt/villus in situ hybridization database", BIOINFORMATICS, vol. 20, no. 8, February 2004 (2004-02-01), pages 1327 - 1328 *

Also Published As

Publication number Publication date
WO2007030373A2 (en) 2007-03-15

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