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WO2007011622A3 - Microfluidic methods and apparatuses for sample preparation and analysis - Google Patents

Microfluidic methods and apparatuses for sample preparation and analysis Download PDF

Info

Publication number
WO2007011622A3
WO2007011622A3 PCT/US2006/027111 US2006027111W WO2007011622A3 WO 2007011622 A3 WO2007011622 A3 WO 2007011622A3 US 2006027111 W US2006027111 W US 2006027111W WO 2007011622 A3 WO2007011622 A3 WO 2007011622A3
Authority
WO
WIPO (PCT)
Prior art keywords
fluid
targets
apparatuses
analysis
carrier fluid
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/US2006/027111
Other languages
French (fr)
Other versions
WO2007011622A2 (en
Inventor
Gregory R Yantz
Jonathan W Larson
Rudolf Gilmanshin
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
US Genomics Inc
Original Assignee
US Genomics Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by US Genomics Inc filed Critical US Genomics Inc
Priority to JP2008522826A priority Critical patent/JP2009501938A/en
Priority to EP06787065A priority patent/EP1904653A4/en
Publication of WO2007011622A2 publication Critical patent/WO2007011622A2/en
Publication of WO2007011622A3 publication Critical patent/WO2007011622A3/en
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

Links

Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B82—NANOTECHNOLOGY
    • B82Y—SPECIFIC USES OR APPLICATIONS OF NANOSTRUCTURES; MEASUREMENT OR ANALYSIS OF NANOSTRUCTURES; MANUFACTURE OR TREATMENT OF NANOSTRUCTURES
    • B82Y10/00—Nanotechnology for information processing, storage or transmission, e.g. quantum computing or single electron logic
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502761—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip specially adapted for handling suspended solids or molecules independently from the bulk fluid flow, e.g. for trapping or sorting beads, for physically stretching molecules
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502769—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by multiphase flow arrangements
    • B01L3/502776—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by multiphase flow arrangements specially adapted for focusing or laminating flows
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B82—NANOTECHNOLOGY
    • B82Y—SPECIFIC USES OR APPLICATIONS OF NANOSTRUCTURES; MEASUREMENT OR ANALYSIS OF NANOSTRUCTURES; MANUFACTURE OR TREATMENT OF NANOSTRUCTURES
    • B82Y5/00—Nanobiotechnology or nanomedicine, e.g. protein engineering or drug delivery
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/06—Fluid handling related problems
    • B01L2200/0636—Focussing flows, e.g. to laminate flows
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/06—Fluid handling related problems
    • B01L2200/0647—Handling flowable solids, e.g. microscopic beads, cells, particles
    • B01L2200/0663—Stretching or orienting elongated molecules or particles
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/04—Moving fluids with specific forces or mechanical means
    • B01L2400/0475—Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure
    • B01L2400/0487—Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure fluid pressure, pneumatics

Landscapes

  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Nanotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Clinical Laboratory Science (AREA)
  • Hematology (AREA)
  • Analytical Chemistry (AREA)
  • Dispersion Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Pharmacology & Pharmacy (AREA)
  • General Engineering & Computer Science (AREA)
  • Medicinal Chemistry (AREA)
  • Medical Informatics (AREA)
  • Mathematical Physics (AREA)
  • Theoretical Computer Science (AREA)
  • Fluid Mechanics (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Automatic Analysis And Handling Materials Therefor (AREA)
  • Physical Or Chemical Processes And Apparatus (AREA)

Abstract

Microfludic channels are constructed for use in preparing and/or analyzing samples. In one embodiment, a microfluidic channel receives a carrier fluid having both non-targets and targets. The non-targets are moved from the carrier fluid by diffusion and into sheathing fluids also present in the channel before contents of the carrier fluid are analyzed. In another embodiment, a microchannel delivers multiple fluid samples from an upstream portion to a downstream portion of the channel where each of the fluid samples are separated from one another by a sheathing fluid that also travels toward the downstream portion. In another embodiment, a microchannel is constructed to create multiple elongational flows in series that can align polymers from a coiled state.
PCT/US2006/027111 2005-07-18 2006-07-13 Microfluidic methods and apparatuses for sample preparation and analysis Ceased WO2007011622A2 (en)

Priority Applications (2)

Application Number Priority Date Filing Date Title
JP2008522826A JP2009501938A (en) 2005-07-18 2006-07-13 Microfluidic method and microfluidic device for sample preparation and analysis
EP06787065A EP1904653A4 (en) 2005-07-18 2006-07-13 Microfluidic methods and apparatuses for sample preparation and analysis

Applications Claiming Priority (6)

Application Number Priority Date Filing Date Title
US70068905P 2005-07-18 2005-07-18
US70048005P 2005-07-18 2005-07-18
US70049005P 2005-07-18 2005-07-18
US60/700,480 2005-07-18
US60/700,689 2005-07-18
US60/700,490 2005-07-18

Publications (2)

Publication Number Publication Date
WO2007011622A2 WO2007011622A2 (en) 2007-01-25
WO2007011622A3 true WO2007011622A3 (en) 2007-05-24

Family

ID=37669351

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2006/027111 Ceased WO2007011622A2 (en) 2005-07-18 2006-07-13 Microfluidic methods and apparatuses for sample preparation and analysis

Country Status (3)

Country Link
EP (1) EP1904653A4 (en)
JP (1) JP2009501938A (en)
WO (1) WO2007011622A2 (en)

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9360476B2 (en) 2006-12-19 2016-06-07 Fio Corporation Microfluidic system and method to test for target molecules in a biological sample
US9459200B2 (en) 2008-08-29 2016-10-04 Fio Corporation Single-use handheld diagnostic test device, and an associated system and method for testing biological and environmental test samples

Families Citing this family (15)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU2008276027B2 (en) * 2007-07-16 2014-09-04 California Institute Of Technology Arrays, substrates, devices, methods and systems for detecting target molecules
WO2009046540A1 (en) 2007-10-12 2009-04-16 Fio Corporation Flow focusing method and system for forming concentrated volumes of microbeads, and microbeads formed further thereto
WO2009155704A1 (en) 2008-06-25 2009-12-30 Fio Corporation Bio-threat alert system
BRPI1007141A2 (en) 2009-01-13 2018-02-20 Fio Corporation portable diagnostic test device and method for use with an electronic device and test cartridge in a rapid diagnostic test
US8202486B2 (en) * 2009-08-12 2012-06-19 Caliper Life Sciences, Inc. Pinching channels for fractionation of fragmented samples
WO2012040098A2 (en) 2010-09-21 2012-03-29 The University Of North Carolina At Chapel Hill Methods, systems and devices for forming nanochannels
WO2013039778A2 (en) 2011-09-12 2013-03-21 The University Of North Carolina At Chapel Hill Devices with a fluid transport nanochannel intersected by a fluid sensing nanochannel and related methods
EP3591408A1 (en) * 2012-02-10 2020-01-08 The University of North Carolina at Chapel Hill Devices with fluidic nanofunnels, associated methods, fabrication and analysis systems
CN105122070B (en) * 2013-02-28 2018-06-26 北卡罗来纳-查佩尔山大学 Nanofluidic devices with integrated components for controlled capture, trapping and transport of macromolecules and related analytical methods
CN105452482B (en) 2013-03-13 2020-03-06 北卡罗来纳-查佩尔山大学 Nanofluidic devices and associated analytical systems and methods for rapid genome-wide mapping
WO2016182811A1 (en) 2015-05-11 2016-11-17 The University Of North Carolina At Chapel Hill Fluidic devices with nanoscale manifolds for molecular transport, related systems and methods of analysis
CN113474084B (en) 2017-06-28 2024-02-09 生物辐射实验室股份有限公司 Systems and methods for droplet detection
WO2019089979A1 (en) * 2017-11-01 2019-05-09 Bio-Rad Laboratories, Inc. Microfluidic system and method for arranging objects
US10898895B2 (en) 2018-09-13 2021-01-26 Talis Biomedical Corporation Vented converging capillary biological sample port and reservoir
CN113498362B (en) * 2019-02-27 2023-01-20 京瓷株式会社 Particle separation measuring device and particle separation measuring apparatus

Citations (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4793705A (en) * 1987-10-07 1988-12-27 The United States Of America As Represented By The United States Department Of Energy Single molecule tracking
US5274240A (en) * 1990-01-12 1993-12-28 The Regents Of The University Of California Capillary array confocal fluorescence scanner and method
US5356776A (en) * 1991-09-10 1994-10-18 Hitachi, Ltd. DNA measuring method
US5674743A (en) * 1993-02-01 1997-10-07 Seq, Ltd. Methods and apparatus for DNA sequencing
US6355420B1 (en) * 1997-02-12 2002-03-12 Us Genomics Methods and products for analyzing polymers
US6790671B1 (en) * 1998-08-13 2004-09-14 Princeton University Optically characterizing polymers
US6818395B1 (en) * 1999-06-28 2004-11-16 California Institute Of Technology Methods and apparatus for analyzing polynucleotide sequences
US7118910B2 (en) * 2001-11-30 2006-10-10 Fluidigm Corporation Microfluidic device and methods of using same

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1179087B1 (en) * 1999-05-17 2019-03-27 Caliper Life Sciences, Inc. Focusing of microparticles in microfluidic systems
US6770182B1 (en) * 2000-11-14 2004-08-03 Sandia National Laboratories Method for producing a thin sample band in a microchannel device
AU2002310500A1 (en) * 2001-06-20 2003-01-08 Cytonome, Inc. Microfluidic system including a virtual wall fluid interface port for interfacing fluids with the microfluidic system
WO2003066191A1 (en) * 2002-02-04 2003-08-14 Colorado School Of Mines Laminar flow-based separations of colloidal and cellular particles
EP1380337B1 (en) * 2002-07-12 2012-11-14 Tosoh Corporation Fine channel device and a chemically operating method for fluid using the device
WO2004091795A2 (en) * 2003-04-10 2004-10-28 U.S. Genomics, Inc. Manipulation of polymers in a microchannel

Patent Citations (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4793705A (en) * 1987-10-07 1988-12-27 The United States Of America As Represented By The United States Department Of Energy Single molecule tracking
US5274240A (en) * 1990-01-12 1993-12-28 The Regents Of The University Of California Capillary array confocal fluorescence scanner and method
US5356776A (en) * 1991-09-10 1994-10-18 Hitachi, Ltd. DNA measuring method
US5674743A (en) * 1993-02-01 1997-10-07 Seq, Ltd. Methods and apparatus for DNA sequencing
US6355420B1 (en) * 1997-02-12 2002-03-12 Us Genomics Methods and products for analyzing polymers
US6790671B1 (en) * 1998-08-13 2004-09-14 Princeton University Optically characterizing polymers
US6818395B1 (en) * 1999-06-28 2004-11-16 California Institute Of Technology Methods and apparatus for analyzing polynucleotide sequences
US6911345B2 (en) * 1999-06-28 2005-06-28 California Institute Of Technology Methods and apparatus for analyzing polynucleotide sequences
US7118910B2 (en) * 2001-11-30 2006-10-10 Fluidigm Corporation Microfluidic device and methods of using same

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
See also references of EP1904653A4 *

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9360476B2 (en) 2006-12-19 2016-06-07 Fio Corporation Microfluidic system and method to test for target molecules in a biological sample
US9459200B2 (en) 2008-08-29 2016-10-04 Fio Corporation Single-use handheld diagnostic test device, and an associated system and method for testing biological and environmental test samples

Also Published As

Publication number Publication date
EP1904653A2 (en) 2008-04-02
JP2009501938A (en) 2009-01-22
WO2007011622A2 (en) 2007-01-25
EP1904653A4 (en) 2010-04-14

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