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WO2007005349A2 - Procede de traitement d'holothuries, avec nouvelle composition de matiere - Google Patents

Procede de traitement d'holothuries, avec nouvelle composition de matiere Download PDF

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Publication number
WO2007005349A2
WO2007005349A2 PCT/US2006/024684 US2006024684W WO2007005349A2 WO 2007005349 A2 WO2007005349 A2 WO 2007005349A2 US 2006024684 W US2006024684 W US 2006024684W WO 2007005349 A2 WO2007005349 A2 WO 2007005349A2
Authority
WO
WIPO (PCT)
Prior art keywords
water
sea cucumber
processing
produce
filtration
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/US2006/024684
Other languages
English (en)
Other versions
WO2007005349A3 (fr
WO2007005349A8 (fr
Inventor
Peter D. Collin
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Coastside Bio Resources
Original Assignee
Coastside Bio Resources
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Coastside Bio Resources filed Critical Coastside Bio Resources
Publication of WO2007005349A2 publication Critical patent/WO2007005349A2/fr
Publication of WO2007005349A8 publication Critical patent/WO2007005349A8/fr
Publication of WO2007005349A3 publication Critical patent/WO2007005349A3/fr
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

Links

Classifications

    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/185—Magnoliopsida (dicotyledons)
    • A61K36/42—Cucurbitaceae (Cucumber family)
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23J—PROTEIN COMPOSITIONS FOR FOODSTUFFS; WORKING-UP PROTEINS FOR FOODSTUFFS; PHOSPHATIDE COMPOSITIONS FOR FOODSTUFFS
    • A23J1/00—Obtaining protein compositions for foodstuffs; Bulk opening of eggs and separation of yolks from whites
    • A23J1/001—Obtaining protein compositions for foodstuffs; Bulk opening of eggs and separation of yolks from whites from waste materials, e.g. kitchen waste
    • A23J1/002—Obtaining protein compositions for foodstuffs; Bulk opening of eggs and separation of yolks from whites from waste materials, e.g. kitchen waste from animal waste materials
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23J—PROTEIN COMPOSITIONS FOR FOODSTUFFS; WORKING-UP PROTEINS FOR FOODSTUFFS; PHOSPHATIDE COMPOSITIONS FOR FOODSTUFFS
    • A23J1/00—Obtaining protein compositions for foodstuffs; Bulk opening of eggs and separation of yolks from whites
    • A23J1/04—Obtaining protein compositions for foodstuffs; Bulk opening of eggs and separation of yolks from whites from fish or other sea animals
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K10/00—Animal feeding-stuffs
    • A23K10/20—Animal feeding-stuffs from material of animal origin
    • A23K10/26—Animal feeding-stuffs from material of animal origin from waste material, e.g. feathers, bones or skin
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs

Definitions

  • the present invention relates generally to methods of processing sea cucumbers and, more particularly, to improved sea cucumber processing methods are disclosed that minimize water use, improve air, water and land contamination, produce marketable products, and produce distilled water as a by product .
  • Sea cucumbers are processed world-wide for their body-wall meat, and for their longitudinal muscle meat, as well as occasionally for production of fermented parts of the intestine, especially the gonadal tissue, referred to as Konowata in Japan. Processing, in most instances, involves manual cutting open of the body wall of the animal, excision of the guts, excision of the muscle meat either manually or by machine. The muscle meat is usually frozen, and the body wall is usually boiled and dried for the Asian market.
  • the traditional processing includes manual cutting and draining of the intestinal Coelemic fluids into fresh water flumes that carry that same water to discharge overboard into the sea. Likewise, the gut contents and top and/or bottom pieces of the animal are cut and scraped manually. These portions of the animal are then ' discarded into the same fresh water flume whereby those solid pieces must eventually be separated before ocean discharge, with the water passing as treated or untreated into the sea.
  • the Cucumaria frondosa processing industry also involves the cooking of the skins of the animal prior to drying. Since water weight of the animal is decreased and adds to the cooking water volume, there is continual boiling over of fresh cooking water into drains commonly used for all discharge wastes. Since this boiling water contains potentially fish-toxic glycosides, this water should not be considered benign to the environment, and should be contained or commercially utilized in some manner. Either at the end of the processing day or throughout the day, the water is drained and/or re-filled.
  • This vacuum air-flow ducting is divided under or over the worker area, the intake suction of which is near the source of origin of aerosolized contamination of breathing or skin contact air, and is ducted though hydrophilic filters and/or heat exchangers to prevent undue loss of building heat, and finally ducted outdoors, such process of ventilation is known to those skilled in the the 'ventilation' sciences, but is a novel step brought to bear upon the aerosolized contaminants plagueing the sea cucumber -A- processing industry presently.
  • the field of this invention is the sea cucumber processing industry wherein processing and waste disposal challenges hamper economical net profit return on investment dollars.
  • the waste disposal represents a considerable expense, despite patents describing the novel use of some of the particular wastes (Collin patents) .
  • the intestinal wastes if contacted extensively with water that will eventually be discharged into bodies of water such as the sea or fresh-water streams, ponds or lakes, presents a certain amount of 'fish toxicity 1 inasmuch as there exist toxins in most sea cucumbers that increase mortality in fish.
  • An object of the present invention is an improved method for processing Cucumaria frondosa or other sea cucumber species, that utilizes an industrial vacuum system for immediate removal ⁇ and storage of intestinal material from the animal .
  • An additional method is disclosed that utilizes a macerating pump prior to solids separations from intestinal mass, in which permeate through a screen produces an aqueous intestinal mass and retentate from screening produces solids for further processing. Said screening of sea cucumber processing wastes can also aid in pumping of said wastes to tanker trucks or other storage and removal equipment.
  • a method is disclosed describing a filtering and evaporation process for reducing cooking water wastes for later use in biomedical industries, and as food additives. A method is described for production of usable protein tissue high in beneficial lipids, produced by screening, evaporation of inherent moisture and grinding.
  • a method of production is disclosed to produce a lipid-bearing protein powder from sea cucumber intestinal material.
  • the product by process has usefulness in the nutritional health sector and is raw material for further processing steps.
  • This present invention is an improvement of traditional methods and is referred to as Vacuum, Evaporation and
  • sea cucumber coelemic fluid can be isolated from any discharge processing water by a simple 'cutting grate' which allows the coelemic fluids of the animal to spill through the grate to a piped catchment apparatus that can then be clarified by known methods, and subsequently filtered by micro, ultra, nanofiltration and then reverse osmosis to obtain a novel biologically active sea cucumber fraction for further commercial exploitation or reduction under reduced pressure to form a powder.
  • a simple 'cutting grate' which allows the coelemic fluids of the animal to spill through the grate to a piped catchment apparatus that can then be clarified by known methods, and subsequently filtered by micro, ultra, nanofiltration and then reverse osmosis to obtain a novel biologically active sea cucumber fraction for further commercial exploitation or reduction under reduced pressure to form a powder.
  • Coelemic fluids are oftentimes found coming from the fish 'totes' in which the animals are transported from the fishing boats. This fluid can accumulate in the chilled holding rooms, in the transportation truck or wherever the animals are stored. Since this fluid contains glycosides of potential medical interest, it is an object of the present invention to provide a means of sequestering these ' waste-coelemic fluids' for future use or merely for safe disposal. Specifically, any source of said sea cucumber coelemic fluids can be identified and said liquid can be pumped to evaporation tanks after traditional clarification of solids, as is commonly done in the waste-water processing industry.
  • the resulting wastes, in a reduced form as a powder or thick salty liquid can be further utilized or when in a liquid form, can be ultra and nanofiltered and finally subjected to reverse osmosis, as is commonly utilized in other industries to form raw materials for medical research or as crude drugs of a particular molecular weight sized class.
  • sea cucumber coelemic fluids contain certain biologically active cytokines and cytokine inhibitors of various molecular weights. It is an object of the present invention to provide a novel means of producing such raw material at an industrial quantity.
  • a vacuum system that can be activated from sensors or manually or can be run non-stop whereby the gut contents can be vacuumed up and discharged into holding tanks or bins, as was widely done in the US poultry industry as a means of quickly removing the poultry entrails from the meat processing environment .
  • the 'meat-washing-water' can be minimized by employment of motion or other sensors on the clamping devise or other sporadically actioned device, wherein activation manually of the clamping or other device begins the ' water flow during the 'meat-brushing' operation. This improvement decreases water usage considerably and decreases the amount of water that needs to be treated or discharged.
  • the brush itself can contain a vacuum ou'tlet that can discharge those 'meat-washing-wastes' to the same vessel or bin as the gut content.
  • a vacuum ou'tlet that can discharge those 'meat-washing-wastes' to the same vessel or bin as the gut content.
  • such 'washing water' can be recycled after clarification, thus reducing the water usage, and the volume of water needed to be treated prior to the waste management component of the industrial process.
  • New methods to reduce water consumption and obtain usable product Cook-water from cooking pots is drained or pumped to clarifiers as are known to persons skilled in the waste-water processing arts, and then to a series of steam-jacketed evaporation tanks that are under vacuum of approximately 20 inches of mercury.
  • the cook-water so processed is relieved of between 50 and 75% of its water weight, removed from the tank as a sludge and further dried by any conventional means, including freeze-drying, rotary vacuum drying, spray drying or fluid-bed drying to produce a powder.
  • This process removes the potential for environmental harm occasioned by cooking water discharge into a waste stream destined for overboard discharge into the sea. It is also a novel method to sequester a raw material for subsequent production of sea cucumber glycosides, lipids and proteins.
  • the cook-water can also be subjected to various filtration processes at any step during its aqueous evaporation process, as might be convenient, depending upon the constraints of any filtration process employed
  • the present invention is intended to provide a means of sequestering sea cucumber glycosides from an aqueous sea cucumber processing waste stream.
  • the following examples are indicative of the improvements to be realized from the present invention: 1.
  • Air exchange improvement In a sea cucumber processing plant, an air removal system is placed in a manifold fashion, with suction outlets positioned near each worker. PVC 4 inch piping, with caps or valving of said piping accommodates the need to take unused worker position suctions off-line if desired.
  • the 4 inch line is connected to forced air suctions sufficient to produce a fume or smoke suction at the worker site of at least two feet of 10 cubic feet per minute. Said line is capable of quick cleaning during plant-clean-up operations by anti-microbial solution washing, ease of take-down, water flooding and the like.
  • Cutting board improvement It is an object of the present invention to provide a process for industrial isolation of sea cucumber coelemic fluids, both with a goal of decreasing contamination and also for the goal of production of a novel raw material for further processing of said coelemic fluids .
  • Worker cutting positions are equipped with a positionable cutting board with a slight containment aspect of a rim around a cutting area of between 3 and 8 inches that has as its base, a plastic removable and cleanable disk with drilled or punched holes, or other means of liquid drainage leading to piping or vessel containment beneath the table.
  • all processing stations on the processing line are equipped with connectivity to a central vessel that can be further pumped to evaporation and/or filtering processes.
  • such vessel can be as simple as a plastic 5 gallon bucket or the like, which is emptied as necessary.
  • a removable and cleanable screen Prior to entry into a piping system or bucket, a removable and cleanable screen can be placed that grossly filters gut pieces from the coelemic liquid being saved.
  • a vacuum pump suitable to create sufficient vacuum in as many worker cutting stations as necessary is attached through cleanable piping to vessels which can contain the sea cucumber processing wastes, as are known to those skilled in the poultry processing arts.
  • the work stations are equipped with suction ends connected to the vacuum system either as flexible wands or as stationary suction ends near the cutting boards. Workers lift -the sea cucumber skin with guts up to the vacuum inlet, or apply the suction end via a flexible hose to the skin area of the animal, or with the cutting knife, scrape the loosened guts towards the vacuum opening, or if cutting is mechanized, contact the vacuum opening to the guts as possible depending upon the configuration of the mechanized operation, bring the vacuum system into contact with the gut wastes or products. 4.
  • the scraped guts are not contacted with water after removal, but are directed to vessels or bins that' do not need the transport properties of the flowing water, as is commonly used. This protects the water from future contamination such as with ichthyotoxins and increased levels of proteins creating biological oxygen demand and related contaminations resulting from processing contact of water with gut contents. 5. Liquifaction of gut contents.
  • the gut contents are churned, agitated or otherwise partly macerated with pumps known to those in the sewage processing arts, which have as an object the liquefaction of the highly aqueous gut contents which is approximately 90% water as it comes from the sea cucumber.
  • the scrapings of guts may also contain the removed top portion of the animal known as the tentacles or 'flower end' and referred to as T-2000 in the patents of Collin, or the removed bottom cap hereinafter referred to as
  • Oil and flotsam debris obtained in this manner is further subjected to filtering of various size mesh screens to remove any foreign material, and is then brought by pipes or flumes to additional centrifuges and clarifiers as are known to the sewage processing arts, that may further separate remaining contaminant/aqueous materials.
  • Oils and oily material thus obtained are piped to oil containers of 20 to 55gallon size, are treated with anti-oxidants such as tartaric acids, or mixed tocopherols, or green tea catechins, or any other suitable anti-oxidant known to the oil-processing arts and are ready for market or further processing.
  • aqueous pigmented layer After oil skimming, the remaining water and tissue sediments are further screened and centrifuged to separate these two additional layers, an aqueous pigmented layer, and a sedimentary tissue layer. Aqueous pigmented layer.
  • Such reduced wastes as described above are then produced into additional useful products, the steps of which can include but are not limited to: a. Rotary drum, tray, heat-pump, air or other types of industrial drying to produce an approximate 10% moisture dry product b. Freeze-drying to produce a 1 -8% dry product c. Tray drying to produce a dry product d. Centrifugation /separation of gut tube wall away from gut contents by common methods which may include cell-wall disruption, grinding, liquefaction and the like. e. Acidification of wastes to stabilize microbial degradation f. Acidification of reduced wastes with water content appropriate for protein precipitation with subsequent additional steps comprising but not limited to: i.
  • Cook-water development Cook-water (CW) is first screened by various sized screens and then clarified as per methods known in the sewage processing arts, (with solids being removed and disposed of appropriately) and the clarified water is then directed to vacuum evaporation vessels for reduction of the water content to a desired percentage, with optional additional steps including but not limited to: a. Micro-ultra and nanofiltration with reverse osomosis to remove protein and/or lipidic compounds for commercial ends i. Specifically, microfiltration is utilized on crude clarified cook-water to remove lipids and proteins entrained in the water, which are sequestered as retentate ii .
  • ultra-filtration uses as starting material the permeate from previous step of micro-filtration, with molecular weight cut-offs of between 2,000 and 30,000 to remove as retentates, proteins or peptides of commercial value, and especially a filtration step consisting of passing the permeate from a 2,000 mw filter through a filter of between 500mw and 1,500 approximate mw to obtain glycosides in the range of 800 to 1400mw. iii . It is an object of the present invention to provide a means of cleaning sea cucumber processing water that has been contaminated with fish toxins related to glycosides.
  • the saponin-bearing extract can be complexed with cholesterol in ethanol or other liquid solvent, with additional steps of organic solvent or supercritical CO2 separation of the cholesterol from the complexed polar saponin, the steps of which may include: a. Addition of saponin bearing extract from above to cholesterol (1:15) in an organic solvent, with agitation at room temperatures for 5 hours. b. Evaporation of said solvent to produce a complex of saponins and cholesterol c. Separation and isolation of saponins from cholesterols by removal of cholesterol by non-polar solvents as are known to the chemistry arts.
  • the Collin patent provides a novel means of producing anti-inflammatory compounds by means of utilizing a 'de-boner/mincer, and sequestering the outer epithelial layer away from the inner endothelial layer. The same method is used to produce a non-bitter protein product of the gut wastes.
  • the methods of which include, but are not limited to: a. Drying of the gut wastes by any means, as described in the U.S. Patent No. 6,055,936 and 6,399,105, both issued to Collin, and incorporated herein by reference as if fully rewritten herein. b. Removal of non-polar lipids, as described in the U.S. Patent No.
  • the 'protein powder of the previous step' is then mixed with water and hydrolyzed enzymatically by any means suitable and as known to those skilled in the protein hyrolysate arts, and filtered through a 10,000 molecular weight cut-off filtration step.
  • This step produces as a permeate, a white peptide material if spray dried after this step. Additionally, if in its aqueous 10,000 mw permeate condition it is further filtered through a 2,000mw filter, there will remain as a retentate, a mild-tasting white peptide powder which can be produced through spray drying.
  • the permeate from this step contains glycosides and salts and can be the raw material of a glycoside-bearing commercial material used as is, or as a feed-stock for further purification of any glycoside contained therein.
  • a glycoside-bearing commercial material used as is, or as a feed-stock for further purification of any glycoside contained therein.
  • salts can be removed, thus further purifying the glycoside material.
  • any fishery waste system that utilizes the separation of proteins away from the aqueous ⁇ components of the waste stream, such as what is known as a 'Disolved Air Filtration' system
  • additional steps to utilize commercial products from that protein/lipid separation step can be instituted that include but are not limited to the steps of: i. Sequestering said protein-bearing phase by means known to the sewage-treatment arts ii . Drying said protein-bearing material through any known means available iii.
  • solvent or supercritical or sub-critical co2 extraction arts are known to those skilled in the solvent or supercritical or sub-critical co2 extraction arts.

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Polymers & Plastics (AREA)
  • Natural Medicines & Medicinal Plants (AREA)
  • Food Science & Technology (AREA)
  • Zoology (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • Botany (AREA)
  • Pharmacology & Pharmacy (AREA)
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  • Alternative & Traditional Medicine (AREA)
  • Molecular Biology (AREA)
  • Medical Informatics (AREA)
  • Medicinal Chemistry (AREA)
  • Microbiology (AREA)
  • Mycology (AREA)
  • Animal Husbandry (AREA)
  • Epidemiology (AREA)
  • Animal Behavior & Ethology (AREA)
  • General Health & Medical Sciences (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Marine Sciences & Fisheries (AREA)
  • Biomedical Technology (AREA)
  • Meat, Egg Or Seafood Products (AREA)
  • Coloring Foods And Improving Nutritive Qualities (AREA)

Abstract

Procédés de traitement d'holothurie réduisant au minimum l'utilisation d'eau, réduisant la contamination aérienne, aquatique et terrestre, permettant de produire des produits commercialisables et donnant de l'eau distillée comme sous-produit.
PCT/US2006/024684 2005-06-29 2006-06-26 Procede de traitement d'holothuries, avec nouvelle composition de matiere Ceased WO2007005349A2 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US69465705P 2005-06-29 2005-06-29
US60/694,657 2005-06-29

Publications (3)

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WO2007005349A2 true WO2007005349A2 (fr) 2007-01-11
WO2007005349A8 WO2007005349A8 (fr) 2007-03-15
WO2007005349A3 WO2007005349A3 (fr) 2007-10-04

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PCT/US2006/024684 Ceased WO2007005349A2 (fr) 2005-06-29 2006-06-26 Procede de traitement d'holothuries, avec nouvelle composition de matiere

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Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2012079310A1 (fr) * 2010-12-14 2012-06-21 大连海晏堂生物有限公司 Préparation de composé de concombre de mer et procédé de préparation de celle-ci
WO2012079311A1 (fr) * 2010-12-14 2012-06-21 大连海晏堂生物有限公司 Produit de composé de concombre de mer, procédé de préparation et formes de préparation de celui-ci
WO2014026291A1 (fr) * 2012-08-15 2014-02-20 Genesis Group Inc. Appareil et procédé de traitement de concombre de mer
CN109516526A (zh) * 2019-01-07 2019-03-26 哈尔滨工业大学（威海） 一种零排放海参加工液的处理方法

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2459620B1 (fr) * 1979-06-26 1983-08-05 Agronomique Inst Nat Rech Hydrolisat enzymatique total de proteines de lactoserum, obtention et application
US4882811A (en) * 1986-10-20 1989-11-28 Baader North America Corporation Apparatus for processing fish
US5985330A (en) * 1996-08-05 1999-11-16 Coastside Bio Resources Inhibition of angiogenesis by sea cucumber fractions
US6055936A (en) * 1997-01-21 2000-05-02 Collin; Peter Donald Sea cucumber carotenoid lipid fractions and process
NZ502442A (en) * 1997-08-12 2000-11-24 Nordischer Maschinenbau Method and apparatus for gutting fish

Cited By (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2012079310A1 (fr) * 2010-12-14 2012-06-21 大连海晏堂生物有限公司 Préparation de composé de concombre de mer et procédé de préparation de celle-ci
WO2012079311A1 (fr) * 2010-12-14 2012-06-21 大连海晏堂生物有限公司 Produit de composé de concombre de mer, procédé de préparation et formes de préparation de celui-ci
US8927523B2 (en) 2010-12-14 2015-01-06 Dalian Haiyantang Biology Co., Ltd. Compound sea cucumber preparation and manufacturing method thereof
US8927008B2 (en) 2010-12-14 2015-01-06 Dalian Haiyantang Biology Co., Ltd. Compound sea cucumber product, preparation method, and dosage forms thereof
WO2014026291A1 (fr) * 2012-08-15 2014-02-20 Genesis Group Inc. Appareil et procédé de traitement de concombre de mer
EP2884849A4 (fr) * 2012-08-15 2016-04-27 Genesis Group Inc Appareil et procédé de traitement de concombre de mer
US9485999B2 (en) 2012-08-15 2016-11-08 Memorial University Of Newfoundland Sea cucumber processing apparatus and method
US9609881B2 (en) 2012-08-15 2017-04-04 Memorial University Of Newfoundland Sea cucumber processing apparatus and method
CN109516526A (zh) * 2019-01-07 2019-03-26 哈尔滨工业大学（威海） 一种零排放海参加工液的处理方法

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Publication number Publication date
WO2007005349A3 (fr) 2007-10-04
WO2007005349A8 (fr) 2007-03-15

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