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WO2007002367A3 - Procede d'extraction de donnees d'intensite a partir d'une image numerisee - Google Patents

Procede d'extraction de donnees d'intensite a partir d'une image numerisee Download PDF

Info

Publication number
WO2007002367A3
WO2007002367A3 PCT/US2006/024432 US2006024432W WO2007002367A3 WO 2007002367 A3 WO2007002367 A3 WO 2007002367A3 US 2006024432 W US2006024432 W US 2006024432W WO 2007002367 A3 WO2007002367 A3 WO 2007002367A3
Authority
WO
WIPO (PCT)
Prior art keywords
optical
pixels
roi
signal intensity
signal
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/US2006/024432
Other languages
English (en)
Other versions
WO2007002367A2 (fr
Inventor
Stephen J Gunstream
Patrick D Kinney
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Applied Biosystems Inc
Original Assignee
Applera Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Applera Corp filed Critical Applera Corp
Priority to EP06773829A priority Critical patent/EP1904954A2/fr
Publication of WO2007002367A2 publication Critical patent/WO2007002367A2/fr
Publication of WO2007002367A3 publication Critical patent/WO2007002367A3/fr
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G06—COMPUTING OR CALCULATING; COUNTING
    • G06T—IMAGE DATA PROCESSING OR GENERATION, IN GENERAL
    • G06T5/00—Image enhancement or restoration
    • G06T5/73—Deblurring; Sharpening
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
    • G01N21/25—Colour; Spectral properties, i.e. comparison of effect of material on the light at two or more different wavelengths or wavelength bands
    • G01N21/251—Colorimeters; Construction thereof
    • G01N21/253—Colorimeters; Construction thereof for batch operation, i.e. multisample apparatus
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/645—Specially adapted constructive features of fluorimeters
    • G01N21/6452—Individual samples arranged in a regular 2D-array, e.g. multiwell plates
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/645—Specially adapted constructive features of fluorimeters
    • G01N21/6456—Spatial resolved fluorescence measurements; Imaging
    • G—PHYSICS
    • G06—COMPUTING OR CALCULATING; COUNTING
    • G06T—IMAGE DATA PROCESSING OR GENERATION, IN GENERAL
    • G06T2207/00—Indexing scheme for image analysis or image enhancement
    • G06T2207/20—Special algorithmic details
    • G06T2207/20212—Image combination
    • G06T2207/20224—Image subtraction
    • G—PHYSICS
    • G06—COMPUTING OR CALCULATING; COUNTING
    • G06T—IMAGE DATA PROCESSING OR GENERATION, IN GENERAL
    • G06T2207/00—Indexing scheme for image analysis or image enhancement
    • G06T2207/30—Subject of image; Context of image processing
    • G06T2207/30004—Biomedical image processing
    • G06T2207/30072—Microarray; Biochip, DNA array; Well plate

Landscapes

  • Physics & Mathematics (AREA)
  • Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Chemical & Material Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • General Health & Medical Sciences (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Spectroscopy & Molecular Physics (AREA)
  • Engineering & Computer Science (AREA)
  • Theoretical Computer Science (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)

Abstract

L'invention concerne un procédé permettant de réduire la diaphonie optique dans un détecteur à réseau optique. Dans divers modes de réalisation, le procédé peut consister à mesurer une émission optique à l'intérieur d'une première région d'intérêt (ROI) au moyen d'une première pluralité de pixels du détecteur à réseau optique, chaque pixel faisant partie de la première pluralité de pixels fournissant une valeur d'intensité de signal optique à l'intérieur de la première ROI. Un signal de somme de ROI peut ainsi être calculé par ajout des valeurs d'intensité de signal optique mesurée par la première pluralité de pixels. Une émission optique à l'intérieur d'une deuxième ROI peut être mesurée à l'aide d'une deuxième pluralité de pixels du détecteur à réseau optique, chaque pixel faisant partie de la deuxième pluralité de pixels fournissant une valeur d'intensité de signal optique à l'intérieur de la deuxième ROI. Les valeurs d'intensité de signal optique fournies par la deuxième pluralité de pixels peuvent être manipulées de manière algébrique afin que soit déterminé un signal de diaphonie optique.
PCT/US2006/024432 2005-06-23 2006-06-21 Procede d'extraction de donnees d'intensite a partir d'une image numerisee Ceased WO2007002367A2 (fr)

Priority Applications (1)

Application Number Priority Date Filing Date Title
EP06773829A EP1904954A2 (fr) 2005-06-23 2006-06-21 Procede d'extraction de donnees d'intensite a partir d'une image numerisee

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US11/166,957 US20060291706A1 (en) 2005-06-23 2005-06-23 Method of extracting intensity data from digitized image
US11/166,957 2005-06-23

Publications (2)

Publication Number Publication Date
WO2007002367A2 WO2007002367A2 (fr) 2007-01-04
WO2007002367A3 true WO2007002367A3 (fr) 2007-12-21

Family

ID=37567402

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2006/024432 Ceased WO2007002367A2 (fr) 2005-06-23 2006-06-21 Procede d'extraction de donnees d'intensite a partir d'une image numerisee

Country Status (3)

Country Link
US (1) US20060291706A1 (fr)
EP (1) EP1904954A2 (fr)
WO (1) WO2007002367A2 (fr)

Families Citing this family (25)

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Publication number Priority date Publication date Assignee Title
US7805081B2 (en) 2005-08-11 2010-09-28 Pacific Biosciences Of California, Inc. Methods and systems for monitoring multiple optical signals from a single source
US7692783B2 (en) 2006-02-13 2010-04-06 Pacific Biosciences Of California Methods and systems for simultaneous real-time monitoring of optical signals from multiple sources
US7715001B2 (en) 2006-02-13 2010-05-11 Pacific Biosciences Of California, Inc. Methods and systems for simultaneous real-time monitoring of optical signals from multiple sources
US7995202B2 (en) 2006-02-13 2011-08-09 Pacific Biosciences Of California, Inc. Methods and systems for simultaneous real-time monitoring of optical signals from multiple sources
EP2064488B1 (fr) 2006-09-01 2021-10-06 Pacific Biosciences of California, Inc. Dispositif et procédé d'analyse de matériaux
US8207509B2 (en) 2006-09-01 2012-06-26 Pacific Biosciences Of California, Inc. Substrates, systems and methods for analyzing materials
US8462377B2 (en) * 2007-07-25 2013-06-11 Aptina Imaging Corporation Method, apparatus, and system for reduction of line processing memory size used in image processing
US20090067700A1 (en) * 2007-09-10 2009-03-12 Riverain Medical Group, Llc Presentation of computer-aided detection/diagnosis (CAD) results
FI3629011T3 (fi) 2008-09-16 2024-01-29 Pacific Biosciences California Inc Integroitu optinen laite
ES2680495T3 (es) 2009-09-10 2018-09-07 Diasorin S.P.A. Compensación para interferencia espectral en la amplificación multiplex de ácidos nucleicos
US8994946B2 (en) 2010-02-19 2015-03-31 Pacific Biosciences Of California, Inc. Integrated analytical system and method
EP2933629B1 (fr) 2010-02-19 2019-04-10 Pacific Biosciences Of California, Inc. Système pour mesurer des réactions analytiques avec une douille pour une puce de réseau d'optode
US8508588B2 (en) * 2010-05-19 2013-08-13 General Electric Company Methods and systems for identifying well wall boundaries of microplates
GB201205607D0 (en) * 2012-03-29 2012-05-16 Ltd Technopath Distrib A fluorescence microtitre plate reader
US9372308B1 (en) 2012-06-17 2016-06-21 Pacific Biosciences Of California, Inc. Arrays of integrated analytical devices and methods for production
EP3524964B1 (fr) 2012-12-18 2020-07-15 Pacific Biosciences Of California, Inc. Dispositif d'analyse optique
WO2014130900A1 (fr) 2013-02-22 2014-08-28 Pacific Biosciences Of California, Inc. Eclairage intégré de dispositifs analytiques optiques
TWI498789B (zh) * 2013-05-20 2015-09-01 Lite On Singapore Pte Ltd 近物感測方法、近物感測裝置與其電子裝置
JP6104049B2 (ja) * 2013-05-21 2017-03-29 オリンパス株式会社 画像処理装置、画像処理方法、および画像処理用プログラム
WO2016033207A1 (fr) 2014-08-27 2016-03-03 Pacific Biosciences Of California, Inc. Réseaux de dispositifs analytiques intégrés
WO2016149397A1 (fr) 2015-03-16 2016-09-22 Pacific Biosciences Of California, Inc. Dispositifs et systèmes intégrés pour couplage optique par espace libre
CN107683340A (zh) 2015-05-07 2018-02-09 加利福尼亚太平洋生物科学股份有限公司 多处理器流水线架构
AU2016276980B2 (en) 2015-06-12 2021-09-23 Pacific Biosciences Of California, Inc. Integrated target waveguide devices and systems for optical coupling
KR102322854B1 (ko) * 2017-03-13 2021-11-08 현대자동차주식회사 초음파센서를 이용한 이동물체 검출 장치 및 그 방법과 이를 이용한 경보 시스템
WO2024254202A1 (fr) * 2023-06-06 2024-12-12 Araceli Biosciences Inc. Systèmes et procédés d'atténuation de diaphonie de canal de microscopie à fluorescence

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US5096807A (en) * 1985-03-06 1992-03-17 Murex Corporation Imaging immunoassay detection system with background compensation and its use
US6007994A (en) * 1995-12-22 1999-12-28 Yale University Multiparametric fluorescence in situ hybridization
US5989835A (en) * 1997-02-27 1999-11-23 Cellomics, Inc. System for cell-based screening
EP0983498B1 (fr) * 1997-02-27 2004-05-26 Cellomics, Inc. Systeme de criblage de cellules
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Non-Patent Citations (2)

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YANG Y.H., BUCKLEY M.J., SPEED T.P.: "Analysis of cDNA microarray images", BRIEFINGS IN BIOINFORMATICS, vol. 2, no. 4, 2001, pages 341 - 349, XP002335348 *

Also Published As

Publication number Publication date
EP1904954A2 (fr) 2008-04-02
US20060291706A1 (en) 2006-12-28
WO2007002367A2 (fr) 2007-01-04

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