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WO2007002210A3 - Embryonic stem cell culture compositions and methods of use thereof - Google Patents

Embryonic stem cell culture compositions and methods of use thereof Download PDF

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Publication number
WO2007002210A3
WO2007002210A3 PCT/US2006/024168 US2006024168W WO2007002210A3 WO 2007002210 A3 WO2007002210 A3 WO 2007002210A3 US 2006024168 W US2006024168 W US 2006024168W WO 2007002210 A3 WO2007002210 A3 WO 2007002210A3
Authority
WO
WIPO (PCT)
Prior art keywords
methods
cell culture
stem cell
embryonic stem
culture compositions
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/US2006/024168
Other languages
French (fr)
Other versions
WO2007002210A2 (en
Inventor
Allan J Robins
Thomas C Schulz
Stephen Dalton
E Edward Baetge
Melissa Carpenter
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Robarts Research Institute
University of Georgia Research Foundation Inc UGARF
Cythera Inc
Viacyte Georgia Inc
Original Assignee
Robarts Research Institute
University of Georgia Research Foundation Inc UGARF
Bresagen Inc
Cythera Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Robarts Research Institute, University of Georgia Research Foundation Inc UGARF, Bresagen Inc, Cythera Inc filed Critical Robarts Research Institute
Publication of WO2007002210A2 publication Critical patent/WO2007002210A2/en
Publication of WO2007002210A3 publication Critical patent/WO2007002210A3/en
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0603—Embryonic cells ; Embryoid bodies
    • C12N5/0606—Pluripotent embryonic cells, e.g. embryonic stem cells [ES]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/105—Insulin-like growth factors [IGF]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/115—Basic fibroblast growth factor (bFGF, FGF-2)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/15—Transforming growth factor beta (TGF-β)

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Gynecology & Obstetrics (AREA)
  • Biotechnology (AREA)
  • Reproductive Health (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Developmental Biology & Embryology (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Cell Biology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

The present invention provides compositions and methods for the culture and maintenance of undifferentiated, pluripotent mammalian stem cells. More particularly, the present invention provides a defined media useful in the absence of a feeder layer and in the absence of serum or serum replacement, that comprises an activator of IGF-IR, an activator of an FGF receptor, serum albumin, transferrin, and optionally, a member of the TGF-β family.
PCT/US2006/024168 2005-06-20 2006-06-20 Embryonic stem cell culture compositions and methods of use thereof Ceased WO2007002210A2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US69218605P 2005-06-20 2005-06-20
US60/692,186 2005-06-20

Publications (2)

Publication Number Publication Date
WO2007002210A2 WO2007002210A2 (en) 2007-01-04
WO2007002210A3 true WO2007002210A3 (en) 2007-07-05

Family

ID=37595805

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2006/024168 Ceased WO2007002210A2 (en) 2005-06-20 2006-06-20 Embryonic stem cell culture compositions and methods of use thereof

Country Status (1)

Country Link
WO (1) WO2007002210A2 (en)

Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9018010B2 (en) 2009-11-12 2015-04-28 Technion Research & Development Foundation Limited Culture media, cell cultures and methods of culturing pluripotent stem cells in an undifferentiated state
US9040297B2 (en) 2006-08-02 2015-05-26 Technion Research & Development Foundation Limited Methods of expanding embryonic stem cells in a suspension culture
US9132226B2 (en) 2008-11-14 2015-09-15 Viacyte, Inc. Encapsulation of pancreatic cells derived from human pluripotent stem cells
US9404079B2 (en) 2005-08-29 2016-08-02 Technion Research & Development Foundation Limited Media for culturing stem cells
US9980986B2 (en) 2006-03-02 2018-05-29 Viacyte, Inc. Methods of producing pancreatic hormones

Families Citing this family (22)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2007059007A2 (en) 2005-11-14 2007-05-24 Cythera, Inc. Markers of definitive endoderm
US7541185B2 (en) 2003-12-23 2009-06-02 Cythera, Inc. Methods for identifying factors for differentiating definitive endoderm
US7625753B2 (en) 2003-12-23 2009-12-01 Cythera, Inc. Expansion of definitive endoderm cells
US7985585B2 (en) 2004-07-09 2011-07-26 Viacyte, Inc. Preprimitive streak and mesendoderm cells
US20050266554A1 (en) 2004-04-27 2005-12-01 D Amour Kevin A PDX1 expressing endoderm
MXPA06007252A (en) 2003-12-23 2007-01-23 Cythera Inc Definitive endoderm.
US8647873B2 (en) 2004-04-27 2014-02-11 Viacyte, Inc. PDX1 expressing endoderm
MX2007001772A (en) 2004-08-13 2007-07-11 Univ Georgia Res Found Compositions and methods for self-renewal and differentiation in human embryonic stem cells.
FI20055398A0 (en) 2005-07-08 2005-07-08 Suomen Punainen Risti Veripalv Method for evaluating cell populations
EP3584311A1 (en) 2005-10-27 2019-12-25 Viacyte, Inc. Pdx-1 expressing dorsal and ventral foregut endoderm
CN105802904B (en) 2006-02-23 2021-04-20 维亚赛特公司 Compositions and methods for culturing differentiable cells
DK1999253T3 (en) 2006-03-02 2019-08-12 Viacyte Inc ENDOCRINE PRECURSOR CELLS, PANCREATIC HORMON-EXPRESSING CELLS AND PROCEDURES FOR MANUFACTURING
US11254916B2 (en) 2006-03-02 2022-02-22 Viacyte, Inc. Methods of making and using PDX1-positive pancreatic endoderm cells
WO2009029983A1 (en) * 2007-09-04 2009-03-12 Queensland University Of Technology A feeder cell-free culture medium and system
US7695963B2 (en) 2007-09-24 2010-04-13 Cythera, Inc. Methods for increasing definitive endoderm production
US20100278788A1 (en) * 2008-01-11 2010-11-04 Bone Therapeutics, S.A. Osteogenic Differentiation Of Bone Marrow Stem Cells And Mesenchymal Stem Cells Using A Combination Of Growth Factors
US8895300B2 (en) 2008-11-04 2014-11-25 Viacyte, Inc. Scalable primate pluripotent stem cell aggregate suspension culture and differentiation thereof
US8008075B2 (en) 2008-11-04 2011-08-30 Viacyte, Inc. Stem cell aggregate suspension compositions and methods of differentiation thereof
US9109245B2 (en) 2009-04-22 2015-08-18 Viacyte, Inc. Cell compositions derived from dedifferentiated reprogrammed cells
JP6148429B2 (en) * 2011-01-31 2017-06-14 協和発酵バイオ株式会社 Method for culturing human pluripotent stem cells
GB201700504D0 (en) * 2017-01-11 2017-02-22 Francis Crick Inst Ltd Composition
CN112920995A (en) * 2021-03-31 2021-06-08 赵峻岭 Mesenchymal stem cell culture serum refueling bag and application thereof

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20020160455A1 (en) * 1999-08-25 2002-10-31 Immunex Corporation Methods and host cells for improved cell culture
US20050037492A1 (en) * 2000-01-11 2005-02-17 Chunhui Xu Medium for growing human embryonic stem cells

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20020160455A1 (en) * 1999-08-25 2002-10-31 Immunex Corporation Methods and host cells for improved cell culture
US20050037492A1 (en) * 2000-01-11 2005-02-17 Chunhui Xu Medium for growing human embryonic stem cells

Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9404079B2 (en) 2005-08-29 2016-08-02 Technion Research & Development Foundation Limited Media for culturing stem cells
US9980986B2 (en) 2006-03-02 2018-05-29 Viacyte, Inc. Methods of producing pancreatic hormones
US9040297B2 (en) 2006-08-02 2015-05-26 Technion Research & Development Foundation Limited Methods of expanding embryonic stem cells in a suspension culture
US9132226B2 (en) 2008-11-14 2015-09-15 Viacyte, Inc. Encapsulation of pancreatic cells derived from human pluripotent stem cells
US9018010B2 (en) 2009-11-12 2015-04-28 Technion Research & Development Foundation Limited Culture media, cell cultures and methods of culturing pluripotent stem cells in an undifferentiated state

Also Published As

Publication number Publication date
WO2007002210A2 (en) 2007-01-04

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