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WO2006111641A3 - Dispositif et procede d'analyse multiparametrique d'elements microscopiques - Google Patents

Dispositif et procede d'analyse multiparametrique d'elements microscopiques Download PDF

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Publication number
WO2006111641A3
WO2006111641A3 PCT/FR2006/000819 FR2006000819W WO2006111641A3 WO 2006111641 A3 WO2006111641 A3 WO 2006111641A3 FR 2006000819 W FR2006000819 W FR 2006000819W WO 2006111641 A3 WO2006111641 A3 WO 2006111641A3
Authority
WO
WIPO (PCT)
Prior art keywords
rays
measuring space
microscopic elements
interacting
analyzing
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/FR2006/000819
Other languages
English (en)
Other versions
WO2006111641A2 (fr
Inventor
Philippe Nerin
Didier Lefevre
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Horiba ABX SAS
Original Assignee
Horiba ABX SAS
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Horiba ABX SAS filed Critical Horiba ABX SAS
Priority to JP2008507118A priority Critical patent/JP2008537136A/ja
Priority to CN2006800208234A priority patent/CN101194159B/zh
Priority to EP06743693A priority patent/EP1875197A2/fr
Priority to US11/912,055 priority patent/US7777869B2/en
Publication of WO2006111641A2 publication Critical patent/WO2006111641A2/fr
Publication of WO2006111641A3 publication Critical patent/WO2006111641A3/fr
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N15/14—Optical investigation techniques, e.g. flow cytometry
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/02—Investigating particle size or size distribution
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N15/14—Optical investigation techniques, e.g. flow cytometry
    • G01N15/1468—Optical investigation techniques, e.g. flow cytometry with spatial resolution of the texture or inner structure of the particle
    • G01N15/147—Optical investigation techniques, e.g. flow cytometry with spatial resolution of the texture or inner structure of the particle the analysis being performed on a sample stream
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N15/14—Optical investigation techniques, e.g. flow cytometry
    • G01N15/1434—Optical arrangements
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N15/14—Optical investigation techniques, e.g. flow cytometry
    • G01N15/149—Optical investigation techniques, e.g. flow cytometry specially adapted for sorting particles, e.g. by their size or optical properties
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N15/14—Optical investigation techniques, e.g. flow cytometry
    • G01N2015/1477—Multiparameters
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N15/14—Optical investigation techniques, e.g. flow cytometry
    • G01N2015/1486—Counting the particles
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N15/14—Optical investigation techniques, e.g. flow cytometry
    • G01N2015/1493—Particle size
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N2021/6417—Spectrofluorimetric devices
    • G01N2021/6419—Excitation at two or more wavelengths
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N2021/6417—Spectrofluorimetric devices
    • G01N2021/6421—Measuring at two or more wavelengths

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Immunology (AREA)
  • Physics & Mathematics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Dispersion Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Optics & Photonics (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)

Abstract

Un dispositif (DA) d'analyse d'éléments microscopiques, comprend, d'une première part, un espace de mesure (CM) pour des éléments microscopiques à analyser, d'une deuxième part, au moins une source (S) délivrant des rayonnements conjugués au niveau de l'espace de mesure (CM), présentant au moins deux longueurs d'onde d'analyse différentes et destinés à interagir avec les éléments microscopiques dans l'espace de mesure (CM) pour former des rayonnements d'interaction, d'une troisième part, des moyens de codage (M) chargés de coder les rayonnements en amont de l'espace de mesure (CM) avec des codes différents, d'une quatrième part, des moyens de filtrage optique (FO) chargés de filtrer sélectivement les rayonnements d'interaction de fluorescence et/ou de diffusion en fonction de leur longueur d'onde, d'une cinquième part, des moyens de détection (DE, DF) chargés de transformer en signaux électriques une partie au moins des rayonnements d'interaction provenant de l'espace de mesure (CM), et d'une sixième part, des moyens d'analyse (MA) comprenant des moyens de décodage (DRE, DRF) chargés de décoder les signaux électriques afin de permettre la détermination de données représentatives des éléments microscopiques analysés.
PCT/FR2006/000819 2005-04-21 2006-04-14 Dispositif et procede d'analyse multiparametrique d'elements microscopiques Ceased WO2006111641A2 (fr)

Priority Applications (4)

Application Number Priority Date Filing Date Title
JP2008507118A JP2008537136A (ja) 2005-04-21 2006-04-14 微視的要素のマルチパラメトリック分析のための装置及び方法
CN2006800208234A CN101194159B (zh) 2005-04-21 2006-04-14 微观元素多参数分析设备和方法
EP06743693A EP1875197A2 (fr) 2005-04-21 2006-04-14 Dispositif et procede d'analyse multiparametrique d'elements microscopiques
US11/912,055 US7777869B2 (en) 2005-04-21 2006-04-14 Device and method for multiparametric analysis of microscopic elements

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
FR0504005 2005-04-21
FR0504005A FR2884920B1 (fr) 2005-04-21 2005-04-21 Dispositif et procede d'analyse multiparametrique d'elements microscopiques

Publications (2)

Publication Number Publication Date
WO2006111641A2 WO2006111641A2 (fr) 2006-10-26
WO2006111641A3 true WO2006111641A3 (fr) 2007-01-04

Family

ID=34955254

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/FR2006/000819 Ceased WO2006111641A2 (fr) 2005-04-21 2006-04-14 Dispositif et procede d'analyse multiparametrique d'elements microscopiques

Country Status (7)

Country Link
US (1) US7777869B2 (fr)
EP (1) EP1875197A2 (fr)
JP (1) JP2008537136A (fr)
KR (1) KR20080007473A (fr)
CN (1) CN101194159B (fr)
FR (1) FR2884920B1 (fr)
WO (1) WO2006111641A2 (fr)

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FR2956207B1 (fr) * 2010-02-10 2012-05-04 Horiba Abx Sas Dispositif et procede de mesures multiparametriques de microparticules dans un fluide
US9522396B2 (en) 2010-12-29 2016-12-20 S.D. Sight Diagnostics Ltd. Apparatus and method for automatic detection of pathogens
FR2971337B1 (fr) * 2011-02-04 2013-03-01 Horiba Abx Sas Dispositif et procede de mesures multiparametriques de microparticules dans un fluide
GB2492950A (en) * 2011-07-11 2013-01-23 Cambridge Consultants Measuring a luminescent property of a sample using a dual-modulated excitation beam
CA2853093C (fr) 2011-10-21 2020-01-14 Acea Biosciences, Inc. Systeme et procede permettant de detecter une lumiere induite par plusieurs sources d'excitation dans un canal de circulation
WO2013098821A1 (fr) 2011-12-29 2013-07-04 Parasight Ltd. Procédés et systèmes de détection de pathogène dans un prélèvement biologique
EP3869257B1 (fr) 2013-05-23 2024-05-08 S.D. Sight Diagnostics Ltd. Procédé et système d'imagerie de prélèvement cellulaire
IL227276A0 (en) 2013-07-01 2014-03-06 Parasight Ltd A method and system for preparing a monolayer of cells, particularly suitable for diagnosis
US10831013B2 (en) 2013-08-26 2020-11-10 S.D. Sight Diagnostics Ltd. Digital microscopy systems, methods and computer program products
EP3071951B1 (fr) 2013-11-19 2019-10-30 ACEA Biosciences, Inc. Moteur optique pour cytomètre en flux, système de cytomètre en flux et procédés d'utilisation
US10261080B2 (en) 2013-11-19 2019-04-16 Acea Biosciences, Inc. Optical detection system for flow cytometer, flow cytometer system and methods of use
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CN107077732B (zh) 2014-08-27 2020-11-24 思迪赛特诊断有限公司 用于对数字显微镜计算聚焦变化的系统及方法
JP6352750B2 (ja) * 2014-09-26 2018-07-04 シスメックス株式会社 血液分析装置および血液分析方法
JP6370659B2 (ja) * 2014-09-26 2018-08-08 シスメックス株式会社 血液分析装置および血液分析方法
WO2017046799A1 (fr) 2015-09-17 2017-03-23 S.D. Sight Diagnostics Ltd Méthodes et appareil de détection d'entité dans un échantillon corporel
CA3018536A1 (fr) 2016-03-30 2017-10-05 S.D. Sight Diagnostics Ltd Distinction entre les composants d'un echantillon de sang
WO2017195208A1 (fr) 2016-05-11 2017-11-16 S.D. Sight Diagnostics Ltd Conduite de mesures optiques sur un échantillon
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Also Published As

Publication number Publication date
WO2006111641A2 (fr) 2006-10-26
EP1875197A2 (fr) 2008-01-09
CN101194159B (zh) 2012-05-02
FR2884920A1 (fr) 2006-10-27
FR2884920B1 (fr) 2007-08-10
US7777869B2 (en) 2010-08-17
JP2008537136A (ja) 2008-09-11
KR20080007473A (ko) 2008-01-21
CN101194159A (zh) 2008-06-04
US20080283754A1 (en) 2008-11-20

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