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WO2006031248A3 - Use of photopolymerization for amplification and detection of a molecular recognition event - Google Patents

Use of photopolymerization for amplification and detection of a molecular recognition event Download PDF

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Publication number
WO2006031248A3
WO2006031248A3 PCT/US2005/008807 US2005008807W WO2006031248A3 WO 2006031248 A3 WO2006031248 A3 WO 2006031248A3 US 2005008807 W US2005008807 W US 2005008807W WO 2006031248 A3 WO2006031248 A3 WO 2006031248A3
Authority
WO
WIPO (PCT)
Prior art keywords
polymer
amplification
molecular recognition
detection
solution
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/US2005/008807
Other languages
French (fr)
Other versions
WO2006031248A2 (en
Inventor
Hadley Sikes
Christopher Bowman
Ryan Hansen
Robert Kuchta
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
University of Colorado System
Original Assignee
University of Colorado System
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from PCT/US2004/029733 external-priority patent/WO2005024386A2/en
Application filed by University of Colorado System filed Critical University of Colorado System
Priority to EP05725765A priority Critical patent/EP1794326A4/en
Publication of WO2006031248A2 publication Critical patent/WO2006031248A2/en
Publication of WO2006031248A3 publication Critical patent/WO2006031248A3/en
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54306—Solid-phase reaction mechanisms
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase
    • C12Q1/6837—Enzymatic or biochemical coupling of nucleic acids to a solid phase using probe arrays or probe chips

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Immunology (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Hematology (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Urology & Nephrology (AREA)
  • Biomedical Technology (AREA)
  • Zoology (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Pathology (AREA)
  • Biophysics (AREA)
  • General Physics & Mathematics (AREA)
  • Food Science & Technology (AREA)
  • Cell Biology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)

Abstract

The invention provides methods to detect molecular recognition events. The invention also provides methods to detect the presence of or identify a target species based on its interaction with one or more probe species. The methods of the invention are based on amplification of the signal due to each molecular recognition event. The amplification is achieved through photopolymerization, with the polymer formed being associated with the molecular recognition event. In an embodiment, a fluorescent polymer, a magnetic polymer, a radioactive polymer or an electrically conducting polymer can form the basis of detection and amplification. In another embodiment, a polymer gel swollen with a fluorescent solution, a magnetic solution, a radioactive solution or an electrically conducting solution can form the basis of detection and amplification. In another embodiment, sufficient polymer forms to be detectable by visual inspection.
PCT/US2005/008807 2004-09-09 2005-03-16 Use of photopolymerization for amplification and detection of a molecular recognition event Ceased WO2006031248A2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
EP05725765A EP1794326A4 (en) 2004-09-09 2005-03-16 USE OF PHOTOPOLYMERIZATION TO AMPLIFY AND DETECT MOLECULAR RECOGNITION EVENT

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
PCT/US2004/029733 WO2005024386A2 (en) 2003-09-09 2004-09-09 Use of photopolymerization for amplification and detection of a molecular recognition event
USPCT/US04/029733 2004-09-09

Publications (2)

Publication Number Publication Date
WO2006031248A2 WO2006031248A2 (en) 2006-03-23
WO2006031248A3 true WO2006031248A3 (en) 2006-08-03

Family

ID=36060452

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2005/008807 Ceased WO2006031248A2 (en) 2004-09-09 2005-03-16 Use of photopolymerization for amplification and detection of a molecular recognition event

Country Status (2)

Country Link
EP (1) EP1794326A4 (en)
WO (1) WO2006031248A2 (en)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20090163375A1 (en) 2003-09-09 2009-06-25 Bowman Christopher N Use of Photopolymerization for Amplification and Detection of a Molecular Recognition Event
GB0617730D0 (en) * 2006-09-08 2006-10-18 Smart Holograms Ltd Analyte detection
WO2013148498A1 (en) 2012-03-27 2013-10-03 Ventana Medical Systems, Inc. Signaling conjugates and methods of use

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6096369A (en) * 1997-06-28 2000-08-01 Huels Aktiengesellschaft Process for hydrophilicizing the surface of polymeric substrates with a macroinitiator as primer
US6406845B1 (en) * 1997-05-05 2002-06-18 Trustees Of Tuft College Fiber optic biosensor for selectively detecting oligonucleotide species in a mixed fluid sample
US20030236425A1 (en) * 2002-06-19 2003-12-25 Herr Donald E. Polymeric photoinitiators
US20040063146A1 (en) * 2002-09-26 2004-04-01 Kimberly-Clark Worldwide, Inc. Diffraction-based diagnostic devices

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4749647A (en) * 1984-06-22 1988-06-07 Genetic Systems Corporation Polymerization-induced separation assay using recognition pairs
US5019496A (en) * 1989-02-17 1991-05-28 Gerald Oster Photopolymerization diagnostic test composition and method for immunoassay and nucleic acid assay
WO2005024386A2 (en) * 2003-09-09 2005-03-17 The Regents Of The University Of Colorado, A Body Corporate Use of photopolymerization for amplification and detection of a molecular recognition event

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6406845B1 (en) * 1997-05-05 2002-06-18 Trustees Of Tuft College Fiber optic biosensor for selectively detecting oligonucleotide species in a mixed fluid sample
US6096369A (en) * 1997-06-28 2000-08-01 Huels Aktiengesellschaft Process for hydrophilicizing the surface of polymeric substrates with a macroinitiator as primer
US20030236425A1 (en) * 2002-06-19 2003-12-25 Herr Donald E. Polymeric photoinitiators
US20040063146A1 (en) * 2002-09-26 2004-04-01 Kimberly-Clark Worldwide, Inc. Diffraction-based diagnostic devices

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
LI J. ET AL.: "Typing and Subtyping Influenza Virus Using DNA Microarrays and Multiplex Reverse Transcriptase", J. CLIN. MICROBIOL., vol. 39, February 2001 (2001-02-01), pages 696 - 704, XP002998571 *
See also references of EP1794326A4 *

Also Published As

Publication number Publication date
EP1794326A4 (en) 2008-06-11
EP1794326A2 (en) 2007-06-13
WO2006031248A2 (en) 2006-03-23

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