[go: up one dir, main page]

WO2006030455A8 - Polymerase d'adn presentant une activite de deplacement de brins - Google Patents

Polymerase d'adn presentant une activite de deplacement de brins

Info

Publication number
WO2006030455A8
WO2006030455A8 PCT/IS2005/000022 IS2005000022W WO2006030455A8 WO 2006030455 A8 WO2006030455 A8 WO 2006030455A8 IS 2005000022 W IS2005000022 W IS 2005000022W WO 2006030455 A8 WO2006030455 A8 WO 2006030455A8
Authority
WO
WIPO (PCT)
Prior art keywords
polymerases
strand displacement
dna polymerases
displacement activity
species
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/IS2005/000022
Other languages
English (en)
Other versions
WO2006030455A1 (fr
Inventor
Sigridur Hjorleifsdottir
Sveinn Ernstson
Thorarinn Blondal
Arnthor Aevarsson
Gudmundur Oli Hreggvidsson
Jakob Kristjansson
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Prokaria Ltd
Original Assignee
Prokaria Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Prokaria Ltd filed Critical Prokaria Ltd
Priority to US11/662,879 priority Critical patent/US20080311626A1/en
Publication of WO2006030455A1 publication Critical patent/WO2006030455A1/fr
Anticipated expiration legal-status Critical
Publication of WO2006030455A8 publication Critical patent/WO2006030455A8/fr
Ceased legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241—Nucleotidyltransferases (2.7.7)
    • C12N9/1252—DNA-directed DNA polymerase (2.7.7.7), i.e. DNA replicase

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Molecular Biology (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Genetics & Genomics (AREA)
  • Medicinal Chemistry (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Enzymes And Modification Thereof (AREA)

Abstract

L'invention porte sur de nouveaux polymérases d'ADN à déplacement de brins pouvant être utilisés dans des réactions d'amplification de déplacement de brins rapides et efficaces. Les polymérases sont nettement plus thermostables que les polymérases de la technique antérieure et présentent une activité élevée à des températures élevées. L'invention porte aussi sur des gènes codant les polymérases et sur des vecteurs contenant ces gènes. Des polymérases représentatifs de l'invention peuvent être obtenus à partir de souches bactériennes des espèces Thermus antranikianii et Thermus brockianus mais aussi à partir d'échantillons prélevés dans l'environnement par isolation des espèces sources.
PCT/IS2005/000022 2004-09-17 2005-09-19 Polymerase d'adn presentant une activite de deplacement de brins Ceased WO2006030455A1 (fr)

Priority Applications (1)

Application Number Priority Date Filing Date Title
US11/662,879 US20080311626A1 (en) 2004-09-17 2005-09-19 Dna Polymerases Having Strand Displacement Activity

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US61056204P 2004-09-17 2004-09-17
IS7461 2004-09-17
IS746104 2004-09-17
US60/610,562 2004-09-17

Publications (2)

Publication Number Publication Date
WO2006030455A1 WO2006030455A1 (fr) 2006-03-23
WO2006030455A8 true WO2006030455A8 (fr) 2007-05-03

Family

ID=37969691

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/IS2005/000022 Ceased WO2006030455A1 (fr) 2004-09-17 2005-09-19 Polymerase d'adn presentant une activite de deplacement de brins

Country Status (1)

Country Link
WO (1) WO2006030455A1 (fr)

Families Citing this family (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1914303A1 (fr) * 2006-10-09 2008-04-23 Qiagen GmbH ADN polymerases de Thermus eggertssonii
GB0803628D0 (en) 2008-02-28 2008-04-02 Genesys Ltd Enzyme
JP2010161935A (ja) * 2009-01-13 2010-07-29 Fujifilm Corp 核酸増幅反応におけるばらつきを低減する方法
EP2981609B1 (fr) 2013-04-05 2020-04-29 Bioron GmbH Nouvelles adn-polymérases
ES2748457T3 (es) 2014-06-06 2020-03-16 Univ Cornell Procedimiento para identificación y enumeración de cambios en la secuencia de ácido nucleico, expresión, copia o metilación de ADN, usando reacciones combinadas de nucleasa, ligasa, polimerasa y secuenciación
GB201721053D0 (en) 2017-12-15 2018-01-31 Univ I Tromsø - Norges Arktiske Univ DNA polymerases
WO2020185312A2 (fr) 2019-01-25 2020-09-17 President And Fellows Of Harvard College Compositions et procédé de synthèse d'acides nucléiques
CN114761548B (zh) * 2019-10-01 2025-01-07 特罗姆瑟大学-挪威北极圈大学 海洋dna聚合酶i
EP4073246A4 (fr) 2019-12-12 2023-12-06 President And Fellows Of Harvard College Compositions et méthodes pour le codage à barres biomoléculaire dirigé par la lumière
JP2023545038A (ja) 2020-10-06 2023-10-26 プレジデント・アンド・フェロウズ・オブ・ハーバード・カレッジ 中和標的のシグナルオン検出のための方法、組成物、キット、およびシステム
WO2022082200A2 (fr) * 2020-10-16 2022-04-21 Geaenzymes Co. Saturation biochimique de molécules et son utilisation
EP4200444A4 (fr) 2020-10-30 2024-09-25 Singular Genomics Systems, Inc. Procédés et compositions pour réduire la teneur en impuretés nucléotidiques

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1992009689A1 (fr) * 1990-12-03 1992-06-11 Stratagene Adn polymerase i thermostable purifiee de $i(pyrococcus furiosus)
US5648211A (en) * 1994-04-18 1997-07-15 Becton, Dickinson And Company Strand displacement amplification using thermophilic enzymes
US7049101B1 (en) * 1997-08-06 2006-05-23 Diversa Corporation Enzymes having high temperature polymerase activity and methods of use thereof
US7052877B2 (en) * 2001-11-30 2006-05-30 Applera Corporation Thermus brockianus nucleic acid polymerases

Also Published As

Publication number Publication date
WO2006030455A1 (fr) 2006-03-23

Similar Documents

Publication Publication Date Title
EP1350841A3 (fr) ADN polymérase thermostable ou thermoactif ayant une activité 3' - 5' exonucléase atténuée
ATE400663T1 (de) Rekombinase-polymerase-amplifikation
WO2008034110A3 (fr) Utilisation d'enzymes mutantes de type taq polymérase pour l'amplification d'adn en présence d'inhibiteurs de pcr
EP1845160A4 (fr) Procedepour l'amplification d'une sequence de nucleotide
DE60112746D1 (de) Kälteempfindliche dna-polymerasemutanten
WO2004058942A3 (fr) Melanges d'adn-polymerase et leurs utilisations
DK1218542T3 (da) Fremgangsmåder og sammensætninger til lineær isotermisk amplifikation af polynukleotidsekvenser
WO2003064691A3 (fr) Methodes et moyens permettant de manipuler l'acide nucleique
NO20072507L (no) Reagenser og fremgangsmater for a bedre reproduserbarhet og redusere feil priming i PCR-amplifikasjon
WO2002101022A3 (fr) Procede d'amplification rapide de l'adn
WO2006030455A8 (fr) Polymerase d'adn presentant une activite de deplacement de brins
WO2008083393A3 (fr) Compositions et procédés d'utilisation des polymérases clivées
WO2007040592A8 (fr) Production de sequences d'acides nucleiques en parallele et utilisation associee
AU4397101A (en) Method and kit for the detection of schistosomiasis through the polymerase chainreaction
WO2008133713A3 (fr) Procédé et composition pour l'amplification de l'adn
DK1815013T3 (da) Sammensætning til at amplificere nucleinsyrer
WO2003004703A1 (fr) Processus de production de matrice d'adn et processus de production de proteine dans un systeme de synthese de proteine sans cellule a l'aide du premier processus
DE60228089D1 (de) Nukleinsäureamplifikation mit duplexzwischenprodukten
WO2003048310A3 (fr) Polymerases d'acide nucleique de thermus oshimai
AU2003266255A1 (en) Method for amplification of nucleic acids of low complexity
WO2004013305A3 (fr) Ameliorations apportees a des compositions pour l'amplification in vitro d'acides nucleiques
WO2007076143A3 (fr) Ensembles d'amorces specifiques d'especes et identification de sequences d'adn specifiques d'especes au moyen de l'enrichissement de fragments genomiques
WO2008146649A1 (fr) Gel contenant une enzyme, et coffret d'amplification d'acide nucléique
EP1598431A4 (fr) Procede de detection du chlamydia trachomatis et trousse a cet effet
WO2006076010A3 (fr) Procedes et compositions permettant de detecter des bacteries au moyen d'une pcr multiplex

Legal Events

Date Code Title Description
AK Designated states

Kind code of ref document: A1

Designated state(s): AE AG AL AM AT AU AZ BA BB BG BR BW BY BZ CA CH CN CO CR CU CZ DE DK DM DZ EC EE EG ES FI GB GD GE GH GM HR HU ID IL IN IS JP KE KG KM KP KR KZ LC LK LR LS LT LU LV LY MA MD MG MK MN MW MX MZ NA NG NI NO NZ OM PG PH PL PT RO RU SC SD SE SG SK SL SM SY TJ TM TN TR TT TZ UA UG US UZ VC VN YU ZA ZM ZW

AL Designated countries for regional patents

Kind code of ref document: A1

Designated state(s): BW GH GM KE LS MW MZ NA SD SL SZ TZ UG ZM ZW AM AZ BY KG KZ MD RU TJ TM AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HU IE IS IT LT LU LV MC NL PL PT RO SE SI SK TR BF BJ CF CG CI CM GA GN GQ GW ML MR NE SN TD TG

121 Ep: the epo has been informed by wipo that ep was designated in this application
NENP Non-entry into the national phase in:

Ref country code: DE

122 Ep: pct application non-entry in european phase
WWE Wipo information: entry into national phase

Ref document number: 11662879

Country of ref document: US