WO2006085607A1 - Plant component-containing emulsion preparation and method for producing the same - Google Patents

Abstract

It is intended to provide a plant component-containing emulsion preparation that stably contains a water-soluble component derived from a plant that is easily oxidized in the air or water and a method for producing the same. Particularly, it is intended to provide a tea leaf component-containing emulsion preparation that stably contains a plant component in an oil phase, by which various active ingredients in tea leaves typified by catechins can be utilized in a comprehensive manner and the application of tea leaves can be widely expanded. The plant component-containing emulsion preparation is characterized in that the water-soluble component derived from a plant that is easily oxidized in the air or water is dispersed in an oil phase and the oil phase is dispersed in an aqueous phase. Particularly, the water-soluble component derived from a plant is a catechin as a tea leaf component and the catechin is dispersed in the oil phase and the oil phase is dispersed in the aqueous phase.

Description

 Specification

 Plant component-containing emulsion preparation and method for producing the same

 Technical field

 [0001] The present invention relates to an emulsion formulation containing a plant component and a method for producing the same. More specifically, an oZw emulsion formulation in which water-soluble components such as water-soluble catechins that are easily oxidized in the air or water as a plant component are dispersed in the oil phase, and this oil phase is dispersed in the water phase. It relates to the manufacturing method.

 [0002] Plant leaves, stems, fruits, seeds, petals, etc. contain many components that are useful to the human body, and they are unstable that are easily oxidized in the air or water. Many water-soluble ingredients exist. Examples of such water-soluble components lacking acidity stability include, for example, polyphenols such as catechins, which have antioxidant, antibacterial, and deodorizing effects contained in green tea leaves, black tea leaves, coffee, grapes, grapes, For example, anthocyanins that promote the resynthesis of rhodopsin contained in blueberries, blueins, etc., vitamin C that inhibits active oxygen contained in lemons, oranges, etc., and vitamin C that promotes collagen production are not limited.

 The present invention is an emulsion preparation that stably contains such an unstable water-soluble component that is easily oxidized in air or water, and a method for producing the same. The following is a more specific example of tea leaf components. Explained.

 Background art

 [0003] Patent Document 1: Japanese Patent Application Laid-Open No. 2002-142677

 Patent Literature 1: “Science and Biology” vol. 38, No. 2, 2000 (104–114 pages)

[0004] Various pharmacological components are contained in tea leaves such as green tea, which is a natural product. Water-soluble ingredients in tea leaves include catechins, caffeine, ascorbic acid (vitamin C), γ-aminobutyric acid, theanine, vitamin koji, water-soluble dietary fiber, saponin, minerals, etc. There are dietary fiber, carotene, tocopherol (vitamin Ε), protein, chlorophyll, etc. In particular, tea leaves contain a large amount of water-soluble catechins, and container-packed tea beverages containing high strength catechins are generally commercially available (Patent Document 1). According to recent studies on the effects of tekins, catechins It has the effects of improving renal failure, reducing body fat, antioxidant, bactericidal / antibacterial, deodorizing, atopic dermatitis, blood pressure rise, and other pharmacological effects.

 [0005] The catechins contained in green tea include epigallocatechin gallate (EGCg), epicatechin, epigallocatechin, epicatechin gallate and the like. Of these, in particular, EGCg accounts for 50-60% of catechins in green tea, has many phenolic hydroxyl groups in the molecule, and has the strongest anti-oxidative activity among catechins. It is known that a wide range of physiological activities are expected.

 Disclosure of the invention

 Problems to be solved by the invention

 [0006] However, force techins are strong and antioxidative by the radical scavenging action of their phenolic hydroxyl groups, and at the same time are unstable in aqueous solutions due to their properties. In addition, of EGCg taken orally, the amount absorbed from the digestive tract to the body is 5-8% of the intake, and the amount of EGCg transferred into the blood is estimated to be about 2% of the intake. (Non-patent Document 1).

 [0007] According to the study by the inventors of the present invention, it is considered that there are some strong techins that dissolve well in oil rather than water at low temperatures due to their structure. Therefore, if tea leaf components containing force techins are uniformly dispersed in the oil phase and used as an oZw type emulsion formulation, water-soluble catechins are present in the oil phase, so that it is chemically stable. And absorption in the body, and it is a water-soluble preparation, so oral and other ingestion can be expected to be easier.

 [0008] The present invention has been found in various studies on tea leaves as described above, and is a plant that stably contains water-soluble components derived from plants that are easily oxidized in air or water. The purpose is to provide an ingredient-containing emulsion preparation and a method for producing the same. Particularly preferably, the plant component is a tea leaf component, and various active ingredients in tea leaves typified by power tekins are comprehensively used. It is an object of the present invention to provide a tea leaf component-containing emulsion preparation that stably contains tea leaf components in an oil phase, and a method for producing the same, which can broaden the use of tea leaves.

Means for solving the problem That is, the present invention is characterized in that a plant-derived water-soluble component that is easily oxidized in air or water is dispersed in an oil phase, and the oil phase is dispersed in the water phase. It is an emulsion formulation containing plant ingredients. In particular, a plant-derived water-soluble component is a catechin as a tea leaf component, the catechin is dispersed in the oil phase, and the oil phase is dispersed in the water phase. It is an emulsion formulation.

 [0010] Further, the present invention pulverizes a plant material containing a water-soluble component that is easily oxidized in air or water, and performs a fine powder treatment while mixing the pre-ground plant material and an oily base material. The plant fine powder is removed to separate the oily solution, and the obtained oily solution, surfactant, and water are stirred to emulsify or dissolve the oily phase in the aqueous phase. It is a method for producing a plant component-containing emulsion preparation. In particular, it is a method for producing a green tea ingredient-containing emulsion preparation, wherein the plant material is tea leaves and contains tea leaf ingredients as plant ingredients.

 In the plant component-containing emulsion preparation of the present invention, a plant-derived water-soluble component that is easily oxidized in air or water (hereinafter referred to as “plant-derived easily oxidizable water-soluble component”) is an oil phase. An oily phase that is dispersed therein and contains an easily acidic water-soluble component derived from this plant is dispersed in the aqueous phase. Here, the dispersion means dispersion (emulsification) in a turbid or translucent state or dispersion (solubilization) in a transparent state. However, “solubilization” is transparent to the naked eye, but microscopically, there are an oil phase and an aqueous phase, and the oil phase is dispersed in the aqueous phase. In the present invention, the emulsion formulation refers to an emulsion prepared so that it can be used directly or indirectly in the fields of food, cosmetics, pharmaceuticals and the like.

 [0012] In the present invention, an easily oxidizable water-soluble component derived from a plant is dispersed in an oil phase to make it oily in a dosage form, so that when a plant component-containing emulsion preparation is orally administered, skin, When applied to mucous membranes, it can be expected to improve the biomembrane permeability, that is, the absorbability in the body, of plant-derived easily acidic water-soluble components. In general, it is known that water-soluble substances such as ions do not easily pass through biological membranes, but lipid-soluble substances of molecular type pass through biological membranes relatively easily. Therefore, it is considered that the absorption in the body can be increased by wrapping an easily acidic water-soluble component derived from a plant with an oily base material to make it fat-soluble.

[0013] The plant material used in the present invention has hitherto been useful for the human body. The leaves, stems, fruits, seeds, petals, etc. of many plants can be mentioned without exception, but tea leaves can be mentioned as a suitable representative example. Here, tea leaves are leaves of the plant name Theasinensis or its cultivated varieties. Tea leaves include non-fermented teas such as green tea, fermented teas such as black tea, and other semi-fermented teas and incompletely fermented teas, all of which are used as raw materials for producing the plant component-containing emulsion preparation of the present invention. Can be used. In addition, the tea leaves that have been used once remain in a large amount in the so-called tea husk, so that the tea husk can also be used as a raw material. Therefore, the tea leaf as used in the present invention includes such a tea husk.

 The invention's effect

 [0014] In the plant component-containing emulsion preparation of the present invention, since the plant-derived water-soluble component is dispersed in the oil phase, and this oil phase is dispersed in the water phase, an oZw type emulsion is formed. Even if it is an easily acidic water-soluble component such as catechins derived from tea leaves that are easily oxidized in air or water, direct contact with air and water is avoided and it is difficult to oxidize Thus, it is possible to stably maintain this easily oxidizable water-soluble active ingredient over a long period of time.

 [0015] In addition, according to the method for producing a plant component-containing emulsion preparation of the present invention, it is possible to stably maintain an easily acid-soluble water-soluble active ingredient such as strength techkins over a long period of time. A plant component-containing emulsion preparation can be easily produced.

[0016] The plant component-containing emulsion preparation of the present invention is a water-soluble emulsion preparation in which the oil phase is soluble or emulsified in an aqueous phase, so that it can be applied to an easily drinkable skin or mucous membrane as an oral beverage. Is very easy to handle. In addition, the plant-containing emulsion preparation of the present invention is usually water-soluble or fat-based compared to the fact that the easily-acid-soluble water-soluble components derived from plants such as extracted green tea catechins are poorly absorbed by the body. Since soluble plant components are stably dispersed in the oil phase, absorption into the body is remarkably superior, and its usefulness is expected to increase dramatically.

[0017] Further, the plant component-containing emulsion preparation of the present invention can contain a plant component containing a plant-derived easily oxidizable water-soluble component such as catechins in a high concentration in the oil phase. Because it is an emulsion formulation that is particularly excellent in absorbability, it can effectively exert the functions of plant components such as catechins, making it suitable for the food, cosmetics, and pharmaceutical fields. Can be used.

 BEST MODE FOR CARRYING OUT THE INVENTION

 [0018] In the plant component-containing emulsion formulation of the present invention, it is preferable that the oil phase contains 0.01 to 70 g of plant components per lOOg of oil phase. 0.1 to 30 g of plant components per lOOg of oil phase It is particularly preferred to contain from 0.1 to 10 g of plant components per lOOg of oil phase.

 [0019] Further, in the present invention, when the plant-derived easily acid-soluble water-soluble component is tea leaf-derived catechins, the oil phase may contain 10 to 7000 mg of force techins per lOOg oil phase. Preference is given to containing 50 to 500 mg of force techins per lOOg oil phase. It is particularly preferred to contain 80 to 300 mg of force techins per 10 Og oil phase.

 [0020] In the present invention, when the plant-derived plant component is a tea leaf-derived tea leaf component, the tea leaf component-containing emulsion preparation is:

 10 to 7000 mg of catechins, 300 to 1200 g (retinoic equivalent) of vitamin A, 2000 to 12000 g (j8-carotene equivalent) of carotene, 0.5 to 150 mg of ascorbic acid (vitamin C), 6 to 40 mg (a-tocopherol equivalent) of vitamin E, and 25 to 220 mg of chlorophyll, preferably 20 to 500 mg per lOOg of an emulsion formulation containing tea leaves Catechins, 400-1000 g (retinoic equivalent) vitamin A, 3000-8000 g (j8 -carotene equivalent) carotene, 0.8-8 mg ascorbic acid (vitamin C), 10- More preferably contains 30 mg (a-tocopherol equivalent) of vitamin E and 40-200 mg of chlorophyll

[0021] Further, in the plant component-containing emulsion preparation of the present invention, when the plant-derived plant component is a tea leaf component derived from tea leaves, it is preferable that most of the tea leaf components are contained in the oil phase. Is

10 to 7000 mg catechins, 300 to 1200 g (equivalent to retinol) vitamin A, 2000 to 12000 ₈ (| 8-carotene equivalent) carotene, 0.5 to 150 mg per lOOg oil phase More preferably it contains ascorbic acid (vitamin C), 6-40 mg (a-tocopherol equivalent) of vitamin E, and 25-220 mg of chlorophyll, 20 to 500 mg of catechins, 400 to 1000 g (retinol equivalent) of vitamin A, 3000 to 8000; Ζ (| 8-forced ten equivalent) of carotene per lOOg oil phase, 0.8 to 8 mg Ascorbic acid (vitamin C), 10-30 mg (a-tocopherol equivalent) of vitamin E, and 40-200 mg of chlorophyll are particularly preferred! /.

 [0022] Thus, by including a water-soluble tea leaf component such as catechins together with a fat-soluble tea leaf component such as carotenes and vitamin E, the tea leaf component can be suitably ingested in a balanced manner. Further, in the oil phase containing these fat-soluble tea leaf components at the same time, it is possible to uniformly disperse water-soluble power techins.

 [0023] Furthermore, in the tea leaf component-containing emulsion preparation of the present invention that comprehensively contains tea leaf components, both water-soluble vitamin C and fat-soluble vitamin E in the tea leaf component have anti-oxidative activity. Therefore, it can be used synergistically by working simultaneously with the anti-acidic action of catechins. In addition to the tea leaf components, water-soluble vitamin C can be added to the water phase, and fat-soluble vitamin E can be further added to the oil phase. It is possible.

 [0024] In the plant component-containing emulsion preparation of the present invention, it is preferable that the average particle size of the oil droplets is designed to be smaller in order to facilitate ingestion and improve handling of the emulsion preparation. The average particle diameter of the oil droplets in the oil phase is preferably 1000 m or less, more preferably 5 m or less, and even more preferably 1 m or less. In the present invention, the average particle size of the oil droplets is 50 nm to 1000 μm by appropriately adjusting the ratio of the aqueous phase to the oil phase, the type and addition ratio of the surfactant, the dispersion method, and the like. An emulsified plant component-containing emulsion preparation can also be prepared, and a solubilized plant component-containing emulsion preparation having an average oil droplet size of 200 nm or less can also be prepared.

 [0025] In the present invention, as a ratio of the oil phase to the whole plant component-containing emulsion formulation, 0.01 to 74 g of the oil phase is added to the aqueous phase per lOOg of the plant component-containing emulsion formulation depending on the use. Can be dispersed in. For a stable oZw type emulsion formulation, the oily phase per 100 g of the plant component-containing emulsion formulation is more preferably in the range of 0.02 to 20 g, particularly preferably in the range of 0.05 to 10 g.

[0026] The oily base material constituting the oil phase of the plant component-containing emulsion preparation of the present invention is a plant. The oil is not limited as long as it has good compatibility with physical components, but edible oils such as vegetable oils and animal oils are preferable. Here, vegetable oils include sesame oil, rice oil, palm oil, olive oil, peanut oil, coconut oil, rapeseed oil, soybean oil, corn oil, sunflower oil, sunflower oil, cottonseed oil, coconut oil, grape seed oil Examples of animal oils include squalene. Among these, coconut oil containing a large amount of medium chain fatty acid triglycerides is more preferred as an oily base material used for the plant component-containing emulsion preparation of the present invention. Alternatively, a mixture of these can also be used.

 [0027] Further, as the oily base material used for the plant component-containing emulsion preparation of the present invention, it is particularly preferable to use a medium-chain fatty acid triglyceride as a main component. Here, the medium chain fatty acid triglyceride refers to a C8 to C12 saturated fatty acid triglyceride. Since medium-chain fatty acid triglycerides are chemically inert (radical-free), the chemical stability of catechins is dramatically improved in the oil phase containing medium-chain fatty acid triglycerides as the main component compared to that in the aqueous phase. In addition, water-soluble force techins can be more stably dispersed in the oil phase than when an oily base material containing a large amount of other unsaturated fatty acid triglycerides is used. Moreover, medium-chain fatty acid triglycerides can be expected to improve the in vivo absorbability of plant-derived easily acidic water-soluble components such as catechins. The oil phase in the vegetable component-containing emulsion preparation of the present invention preferably contains 50 g or more of medium chain fatty acid triglyceride per 10 g of fat and oil component, more preferably 70 g or more, and particularly preferably 80 g or more. Here, the content of the fatty acid triglyceride can be easily measured by high performance liquid chromatography after separating the plant component-containing emulsion preparation into an oil phase and an aqueous phase.

 In the present invention, purified water, drinking water, ion-exchanged water, and the like can be used as the aqueous base material constituting the aqueous phase. In addition, a tea extract obtained by extracting tea leaves with these aqueous base materials can be used, and if necessary, an aqueous base material blended with a fragrance or the like can be used.

[0029] In the present invention, it is preferable to use a surfactant as an emulsifier or solubilizer in order to disperse the oil phase more stably in the aqueous phase. It is preferable to use 0.01 to 40 g of a surfactant. 0.2 to 20 g of a surfactant is more preferable. It is particularly preferable to use 0.5 to 10 g of a surfactant. preferable. The oZw type plant component-containing emulsion preparation of the present invention is capable of using a surfactant having an HLB value of 7 to 40. The emulsion is basically more stable as a surfactant having a higher HLB value is used. The optimum value for the HLB value of the surfactant used is a force that depends on the compatibility of the water phase, oil phase, and surfactant. In general, the HLB value is more preferably 7-20. 20 is particularly preferred. A surfactant having a relatively high HLB value and a surfactant having a relatively low HLB value can also be used in combination.

[0030] As the surfactant used to dissolve or emulsify the oily solution in the aqueous phase, a surfactant for food addition, cosmetic addition, or pharmaceutical addition can be widely used. Specifically, Nikko Chemical's TWEEN80 (T80: HLB value 15.0), Sigma's TW EEN80 (sT80: HLB value 15.0), Nikko Chemical's polyoxyethylene hydrogenated castor oil 60 (HC060: HLB value 14.0) , Daiichi Kogyo Seiyaku Sugar Ester SS (S—SS: HLB value 19.0), Daiichi Kogyo Seiyaku Sugar Ester F110 (S—F110HLB 11.0), Daiichi Kogyo Seiyaku Sugar Ester F160 (S—F160HLB) The value 15.0) etc. can be used.

 [0031] Various methods can be considered as a method for producing the plant component-containing emulsion preparation of the present invention, but roughly divided, a method of emulsifying or solubilizing an oily solution containing plant components and water by stirring. And a method of emulsifying or solubilizing an oily paste containing plant components and water by stirring. Here, as a method for producing an oily solution containing a plant component, a method for producing an oily solution by dissolving components obtained by extracting plant materials such as tea leaves into an oily base material can be mentioned, In addition, a method of leaching plant components by immersing the finely pulverized plant material itself in an oily base material can be mentioned.

[0032] Extraction of plant components can be performed by a conventional method, and the dried plant material such as tea leaves is powdered or shredded, and then heated to water, steam distilled, or alcohol or a mixture of alcohol and water. It is performed by dipping. Alternatively, components rich in catechins can be obtained in the form of a solution by extraction with water at room temperature for 1 to: LO days and then treatment with alcohol. The extract obtained by force is filtered if necessary, the filtrate is concentrated, and if necessary, it is subjected to a drying process. Concentration is generally by distillation under reduced pressure. Drying includes spray drying, vacuum drying, freeze drying, and the like, which can be appropriately selected and employed. [0033] The plant component obtained by force is mixed and dissolved in an oily base material to obtain an oily solution, and this oily solution is added to water and stirred to emulsify or dissolve, thereby containing the plant component of the present invention. An emulsion formulation can be obtained. The mixing ratio can be appropriately selected. The amount of the plant component per liter of the oily base material is usually 10 to: LOOg, preferably 20 to 50 g. In order to dissolve the plant components in the oily base, ultrasonic treatment or physical means using high-speed stirring is employed. If necessary, other components such as fragrances can be blended with the plant component-containing oily base material obtained by caulking.

 [0034] Examples of the method for producing an oily solution containing a plant component include a method in which plant materials such as tea leaves are directly added to an oily substrate and the plant component is transferred to the oily substrate. In this case, it is preferable that the plant material dried and pulverized in advance and the oily base material are mixed to transfer the plant components to the oily base material. The amount of plant material varies depending on the type, degree of drying, etc.Under normal temperature, oil'14 base material 30 ~ 800g, preferably 100 ~ 500g, more preferably 250 ~ 400g It is. The extraction time is 3 to 48 hours, preferably 6 to 24 hours, more preferably 10 to 18 hours. After extraction, the residue is removed by filtration to obtain an oily solution containing plant components. Furthermore, a plant component-containing emulsion preparation can be obtained by stirring and emulsifying or dissolving the obtained oily solution and water. If necessary, other components such as a fragrance can be added to the oily solution containing plant components and Z or the plant component-containing emulsion preparation.

[0035] After plant materials such as tea leaves that have been crushed in advance are added to the oily base material, mixing with the oily base material and further performing a fine pulverization treatment facilitates the transfer of plant components to the oily base material. An oily solution containing plant components at a high concentration can be obtained. In this case, the fine powdering treatment is performed until the plant material strength is preferably 0.01 to 40 μm, more preferably 1 to 10 μm. The fine pulverization treatment step is preferably performed for 5 to 20 minutes, more preferably for 10 to 15 minutes. If the oily paste containing fine powder of insoluble plant material is removed by filtration or centrifugation after completion of the fine powdering treatment step, an oily solution containing plant components at a high concentration can be separated. . In particular, when the separation is performed using a continuous centrifuge at 6000 to 13500 rpm and a liquid supply amount of 50 to 60 liters Z time, the extraction effect is promoted. According to this method, the process of extracting plant components is simple, and the strength is Catechins and the like can be dissolved in an oily substrate at a high concentration. Furthermore, a plant component-containing emulsion preparation can be obtained by stirring and emulsifying or solubilizing the obtained oily solution and water. Here, the oil phase can contain 10 ~: LOOOmg of catechins per lOOg of oil phase. Even in this case, a fragrance or the like may be blended with the oily solution containing plant components and Z or the plant component-containing emulsion preparation as necessary.

 The plant component-containing emulsion preparation of the present invention can also be obtained by stirring and emulsifying or solubilizing the oily paste containing water and the fine powder of the plant material, which is separated as described above, and water. Here, the oil phase may contain 20 to 7000 mg of force techins per lOOg of oil phase. Even in this case, a fragrance or the like may be added to the oil-based paste containing plant components and Z or the plant component-containing emulsion preparation as necessary.

 [0036] Further, when the obtained oily solution or oily paste and water are dispersed by stirring, a surfactant is further added as an emulsifier or solubilizer so that the emulsified state or the soluble state is obtained. It is possible to obtain an emulsion that lasts more stably.

 [0037] As the dispersion (emulsification or solubilization) treatment, ultrasonic treatment or high-pressure homogenizer treatment can be employed. For example, an emulsion (emulsified formulation) with an average particle size of oil droplets of 180 nm can be prepared by appropriate ultrasonic treatment, and when processed with a high-pressure homogenizer, the average particle size of oil droplets decreases with increasing diameter. However, a formulation having 130 nm could be prepared after 20 treatments. Judging by the observation ability of the emulsion preparation after 1 week, stable emulsion could be prepared even by emulsification by ultrasonic treatment. A high-pressure homogenizer treatment is preferable in order to increase stability in consideration of the particle size of the oil droplets.

 [0038] Hereinafter, the plant component-containing emulsion preparation of the present invention and the production method thereof will be described more specifically with reference to examples using tea leaves, but the present invention is not limited to these embodiments. Absent.

 Example

[0039] Green tea dried to a water content of 5% or less 6. Okg Powdered to / zm. Add 15kg (16.7 liters) of coconut oil to this and further wet ultrafine grinder (Saitama Prefecture, Masuyuki Sangyo Co., Ltd., product name Super Masco Mouth Idar MKZA8-10, grinder diameter 150mm) for about 11 minutes. Fine powdering treatment was performed to obtain a mixture of fine tea leaf powder having a particle size of 4 to 1 O / zm and palm oil. Initially, the temperature of coconut oil was 16 ° C, but it rose to 43 ° C during the fine pulverization process. After the fine pulverization process, the mixture was centrifuged (Tokyo, Sakai Kogyo Co., Ltd., product name TOMO—EDE CANTER PTM006, 6000rpm, 3200G, processing temperature 80.C, feed volume 50 gel. Z time) and the clarified liquid was separated from the solids. A slightly greenish oily solution of 5.6 kg was obtained. The results of analyzing the obtained oily solution by high performance liquid chromatography are shown below.

Epicatechin 18mg / 100g

Epigalocatechin 23mg / 100g

Epicatechin gallate 10mg / 100g

Epigalocatechin gallate 60mg / 100g

Vitamin A (Retino 1) 1 800 gZlOOg

Carotene — Rikiguchi Tento I) 4800 / z gZlOOg

a brute force 400 / z gZlOOg

β glance 10300 / z gZlOOg

Total ascorbic acid 1.3 mg / 100 g

Total Tocopherol 14mg / 100g

a Tocopherol 14mg / 100g

β Tocopherol Not detected

γ— 卜 = ι Ferrol 0.3 mg / 100g

δ Tocopherol Not detected

Total chlorofinole 60mg / 100g

 The fatty acid composition is shown below.

 C6 caproic acid 0.1 quality

C8 strength prillic acid 85. 7 quality: CIO force Puric acid 0.1% by mass

C12 lauric acid 13.7 mass ^_0/0

C16 palmitic acid 0.1 mass ^_0/0

C18 linolenic acid 0.1 mass ^_0/0

 Three

 Other 0.2% by mass

 [0042] Using the obtained oily solution, an emulsion preparation containing tea leaf components was prepared by the following method.

 [0043] TWEEN80 (T80) manufactured by Nikko Chemical Co., polyoxyethylene hydrogenated castor oil 60 (HC060) manufactured by Nikko Chemical Co., Ltd., manufactured by Daiichi Pharmaceutical Co., Ltd. Five types were selected: Sugar Ester SS (S-SS), Daiichi Kogyo Seiyaku Sugar Ester F1 10 (S-F110), Daiichi Kogyo Seiyaku Sugar Ester F160 (S-F160).

 [0044] The concentration of the oil phase in the final obtained tea leaf component-containing emulsion formulation is 1, 5, or 10% by mass, and the surfactant concentration is 6 types or 7 types for each oil phase concentration, respectively. I changed it step by step.

 [0045] A predetermined amount of surfactant was placed in a 50 ml wide-mouth glass bottle, a predetermined amount of oily solution was added, and finally a predetermined amount of purified water was added to make a total amount of 20 g. That is, when the oil phase concentration is 1%, weigh the surfactant concentration in a 5 Oml wide-mouth glass bottle (based on the total amount of 20 g) to 0.1, 0.5, 1, 2, 5 or 10% by mass. Then, an oily solution and purified water were added to make a total amount of 20 g. When the oil phase concentration is 5%, weigh the surfactant concentration to 1, 2, 5, 10, 15 or 20% by mass (relative to the total amount of 20g), add the oily solution and purified water, and add the total amount. 20g. In the case of T80 and HC060, 25% by mass was also added. When the oil phase concentration is 10%, weigh the surfactant concentration to 5, 10, 20, 30, 40 or 50% (based on the total amount of 20 g), add the oily solution and purified water, and add the total amount. 20g.

 [0046] These were emulsified or solubilized by treatment with a probe-type ultrasonic generator (TA1TEC, VP-30S, 20KHz). The ultrasonic wave was oscillated intermittently for 1 minute and 20-second pauses 5 times.

[0047] The prepared tea leaf component-containing emulsion preparation was subjected to a digital optical microscope (manufactured by Shimadzu Corporation). GLBB1500MBlTa, X100), and the ease of dispersion was evaluated based on the uniformity of the particle size of the oil droplets in the emulsion and the presence or absence of separation of the dusting layer. Furthermore, the sample was transferred to a 20 ml sample bottle, stored at 20 ° C for 1 month, and then observed under a microscope. The dispersion stability was evaluated based on the results of oil droplet generation and the tarming phenomenon. The evaluation results are shown in Table 1, Table 2 and Table 3.

 [0048] [Evaluation of dispersibility (immediately after preparation)]

 ◎: Evaluation was made on a four-point scale: one that was satisfactorily soluble, ○: one that was successfully emulsified, Δ: one that was slightly emulsifiable, and X: one that was difficult to emulsify. However, it was impossible to evaluate viscosity: high viscosity and solid: solidified after preparation.

 [0049] [Evaluation of dispersion stability (after one month)]

 The evaluation was made in four grades: ◎: good solubility, ○: stable emulsification, Δ: separation was observed, X: evaluation was impossible.

[0050] [Table 1]

Easiness of dispersion Dispersion stability Example No. Ratio of oil phase Surfactant Ratio of surfactant (immediately after preparation) (after one month)

 1

 2

 Three

 Four

 Five

 6

 Quantity quantity quantity quantity quantity quantity quantity quantity quantity quantity quantity quantity quantity quantity quantity

 HCO60 σι mass%

 8 HCO60 C.5 Mass ¾

 9 HCO60 1% by mass

 10 HCO60 2 mass? 6

 11 HC τ τ T τ TO60 5% by mass

 888888 o o o o o o

Quality t ¾ Quantity Quantity

ooo©® οοοοο oooooo o %%

ooo © ® οοοοο oooooo o

 25 S-F160

 26 S-F160

 27 S-F160

 28 S-F160 ΔΟΟΟΟΟ〇 厶 厶 〇〇〇 厶 厶 29 S-F160 οο © 30 S-F160

Evaluation of dispersibility (immediately after preparation)

 ◎: Good solubilization ○: Good emulsification

 Δ: Somewhat difficult to emulsify X: Difficult to emulsify

Evaluation of dispersion stability (after 1 month)

 ◎: Good solubilization ○: Stable emulsification

△: Separation confirmed X: Unevaluable] Easiness of dispersion Dispersion stability Example Ν Ratio of oil phase Surfactant Ratio of surfactant (immediately after preparation) [after one month]

ΟΟΟ oo 厶 △ o〇〇 △ CοοΟΟ〇 厶 Oοοο Δ Δ XX X X XXX X

o Α 厶 ΔΛΔΔΔΔ 厶 "厶 厶 ΔΔΔ〇Ο 厶 厶 ΟxΔ 厶 <xxΧΧΧΧΧ

Evaluation of dispersibility (immediately after preparation)

 ◎: Good solubilization ○: Good emulsification

 厶: Slightly difficult to emulsify X: Difficult to emulsify

Evaluation of dispersion stability (after 1 month)

 ◎: Good solubilization Ο: Emulsified stably

厶: Separation confirmed X: Unevaluable Easiness of dispersion Dispersion stability Example Ν Ratio of oil phase Surfactant Ratio of surfactant (immediately after preparation) (after one month)

 63

 688839 88 38994358 ο 224 791 1

 65

 66 Amount Amount Amount Amount Amount Amount Amount Amount Amount Amount Ft I

τ τ τ τ

2345234511 5 5 3 sssoooo

2345234511 5 5

 ^ ^ ο ο ο ο o o o o o

 -F1 10 § | ^ ^ Quantity Quantity Quantity Quantity Quantity Quantity Quantity 厶 S-F1 10 X S-F1 10 X S-F1 10 X S-F1 10 X S-F1 10

 Viscosity Viscosity 00 Hard solid 0 A △ Δ Solid O viscous X Λ- X X X X X X X X

 S-F160

 S-F160

 S-F160

 S-F160

 S-F160 Δ △-Δ 厶 △△△ <X XXX X X X X X S-F160

 Evaluation of dispersibility (immediately after preparation)

 ◎: Good solubilization Ο: Good emulsification

 厶: Slightly difficult to emulsify X: Difficult to emulsify

 Viscosity: Highly viscous Solid: Solidified after preparation

 Evaluation of dispersion stability (after 1 month)

 ©: Good solubilization Ο: Stable emulsification

 厶: Separation confirmed X: Evaluation not possible

The emulsion preparation containing No. 58 tea leaf component was separated into an oil phase and an aqueous phase using a membrane filter having a molecular weight cut off of 300,000 (trade name: Molecat, Millipore (under pressure)). The oil phase was green and the aqueous phase was almost colorless and transparent.

In addition, the No. 58 tea leaf ingredient-containing emulsion preparation and the No. 58 tea leaf ingredient-containing emulsion preparation were separated from the separated aqueous phase by high performance liquid chromatography, respectively, with vitamin A (retinol equivalent) and vitamin Ε. (α-tocopherol equivalent), phylloquinone Quantitative analysis of Tamine Kl) and Epigalocatechin Garade, and quantitative analysis of total chlorophyll by absorptiometry (visible), and each of the above ingredients in the No. 58 tea leaf ingredient-containing emulsion formulation is in the oil phase. Whether it exists in the water phase or in the aqueous phase. The results are shown in Table 4.

 [Table 4]

 1: α-tocopher mouth lmg, i3 -tocophere D-l 2.5 mg, a-tocofe ιΠ-l lOrag and

 δ-tocopher D-l lOOmg was defined as a-tocophere D-l equivalent Img, respectively.

 2: Spectrophotometry (visible)

[0055] As can be seen from the results of the analysis of the preparations and their aqueous phases shown in Table 4, all the components to be analyzed, including the easily oxidizable water-soluble component epigallocatechin galade, are in the oil phase. Was found to exist. For this reason, in this No. 58 emulsion preparation containing tea leaf components, the water-soluble components derived from plants exist in the oil phase and are stably present that are not easily oxidized due to being blocked from air and water. It is divided.

[0056] Among the tea leaf component-containing emulsion preparations prepared in this example, optimally soluble No. 18 (oil phase: 1% by mass, S-33: 10% by mass) and optimally emulsified No. 18 were obtained. 58 (oil phase: 5 weight _^0/0, F 160: 2 wt%) for further were treated 20 times in a high pressure homogenizer 500kgZcm ^2.

At this time, the average particle size of oil droplets in the oil phase is about 180 nm without high-pressure homogenizer treatment, and about 130 nm with 20 high-pressure homogenizer treatments in the optimally emulsified No. 58 tea leaf component-containing emulsion preparation. , Gradually as the number of high-pressure homogenizer treatment increases (Fig. 1). In each case, the average particle size was measured again one week after preparation, but there was no change in the size of the oil droplets. Even in the optimally soluble emulsion No. 18 emulsion containing tea leaf components, the average particle size of the oil droplets was about 190 nm without high-pressure homogenizer treatment, and about 80 nm with 20 high-pressure homogenizer treatments. It gradually decreased as the number of treatments increased.

[0057] For No. 18 (oil phase: 1% by mass, S—SS: 10% by mass) and optimally emulsified No. 58 (oil phase: 5% by mass, F160: 2% by mass), a high-pressure homogenizer Treated with lOOOkgZcm ² 1-20 times.

 In this case, the average particle size of the oil droplets in the oil phase is about 180 nm without high-pressure homogenizer treatment, about 80 nm with 20 high-pressure homogenizer treatments, and the number of high-pressure homogenizer treatments with the No. 58 tea leaf component-containing emulsion formulation. It gradually became smaller as it increased. In each case, the average particle size was re-measured one week after preparation, but there was no change in the size of the oil droplets. In addition, even the optimally soluble No. 18 emulsion formulation containing tea leaf components, the average particle size of the oil droplets was about 190 nm without high-pressure homogenizer treatment, and about 70 nm with 20 high-pressure homogenizer treatments. It gradually decreased as the number of treatments increased.

 Industrial applicability

[0058] In the present invention, a plant-derived water-soluble component is dispersed in an oil phase, and this oil phase is dispersed in an aqueous phase to form an oZw type emulsion. Even easily oxidizable water-soluble components that are easily oxidized are not easily oxidized by avoiding direct contact with air or water. Long-term use of easily oxidizable water-soluble active components such as catechins Plant component-containing emulsion preparations that can be stably maintained over a long period of time. In the fields of food, cosmetics, pharmaceuticals, etc., various oxidizable water-soluble ingredients derived from plants are used as active ingredients. Is available for any product.

 Brief Description of Drawings

[0059] [Fig. 1] Fig. 1 is a graph showing the relationship between the number of treatments with a high-pressure homogenizer (pressure: 500 kgZcm ² ) and the average particle size of oil droplets for the No. 58 tea leaf component-containing emulsion formulation. The

Claims

The scope of the claims  [1] A plant component-containing emulsion characterized in that a water-soluble component derived from a plant that is easily oxidized in air or water is dispersed in an oil phase, and the oil phase is dispersed in an aqueous phase. Formulation.  [2] The plant component-containing emulsion preparation according to claim 1, wherein the water-soluble component derived from a plant is a catechin as a tea leaf component. [3] The plant component-containing emulsion preparation according to claim 2, wherein the oil phase contains 0.01 to 70 g of tea leaf component per lOOg of oil phase. [4] The plant component-containing emulsion formulation according to claim 3, wherein the oil phase contains 10 to 7000 mg of force techins per lOOg oil phase. [5] The oil phase is per lOOg of oil phase,  10-7000 mg of catechins,  300-1200 g (retinoinole equivalent) of vitamin A, 2000-12000 ₈ (| 8-carotene equivalent) carotene,  0.5 ~: LOmg ascorbic acid,  6-40 mg (α-tocopherol equivalent) of vitamin Ε,  25-220 mg of chlorophyll,  The plant component-containing emulsion preparation according to claim 4, characterized by comprising: [6] The plant component-containing emulsion preparation according to any one of claims 1 to 5, wherein an average particle diameter of the oil droplets in the oil phase is 1000 μm or less. [7] In any one of claims 1 to 6, characterized in that 0.01 to 74 g of the oil phase per 100 g of the plant component-containing emulsion formulation is dispersed in the water phase. The described plant component-containing emulsion preparation. 8. The plant component-containing emulsion preparation according to any one of claims 1 to 7, wherein the oil phase contains 50 g or more of medium chain fatty acid triglyceride per 100 g of fat and oil component. [9] The oil phase is dispersed in the aqueous phase by 0.01 to 40 g of surfactant per 100 g of emulsion formulation! The plant component-containing emulsion preparation according to any one of claims 1 to 8, which is characterized by being sown. [10] The plant component-containing emulsion preparation according to claim 9, wherein the surfactant has an HLB value of 7 to 40.  [11] A plant material containing a water-soluble component that is easily oxidized in air or water is pulverized, and the pulverized plant material and an oily base material are mixed and finely pulverized to obtain a fine plant powder. Removing the oily solution, and stirring the obtained oily solution, a surfactant, and water to emulsify or solubilize the oily phase in the aqueous phase. A method for producing an ingredient-containing emulsion preparation.  12. The method for producing an emulsion preparation containing a plant component according to claim 11, wherein the plant material is tea leaves.  [13] The process for producing a plant component-containing emulsion according to claim 12, wherein the tea leaves are finely powdered until the average particle size becomes 0.01 to 40 μm.