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WO2006045590A3 - Molecules destinees a l'administration d'arn en brin double a des organismes de parasites - Google Patents

Molecules destinees a l'administration d'arn en brin double a des organismes de parasites Download PDF

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Publication number
WO2006045590A3
WO2006045590A3 PCT/EP2005/011439 EP2005011439W WO2006045590A3 WO 2006045590 A3 WO2006045590 A3 WO 2006045590A3 EP 2005011439 W EP2005011439 W EP 2005011439W WO 2006045590 A3 WO2006045590 A3 WO 2006045590A3
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WO
WIPO (PCT)
Prior art keywords
multidomain
aptamer
stranded rna
rna
nucleotide sequence
Prior art date
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Ceased
Application number
PCT/EP2005/011439
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English (en)
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WO2006045590A2 (fr
Inventor
Thierry Andre Olivier Bogaert
Pascale Feldmann
Geert Plaetinck
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Devgen NV
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Devgen NV
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Filing date
Publication date
Application filed by Devgen NV filed Critical Devgen NV
Priority to EP05801497A priority Critical patent/EP1805308A2/fr
Priority to MX2007004686A priority patent/MX2007004686A/es
Priority to AU2005298827A priority patent/AU2005298827B2/en
Priority to BRPI0516329-3A priority patent/BRPI0516329A/pt
Priority to CA002583334A priority patent/CA2583334A1/fr
Publication of WO2006045590A2 publication Critical patent/WO2006045590A2/fr
Publication of WO2006045590A3 publication Critical patent/WO2006045590A3/fr
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/111—General methods applicable to biologically active non-coding nucleic acids
    • C—CHEMISTRY; METALLURGY
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
    • C—CHEMISTRY; METALLURGY
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C12N15/8218—Antisense, co-suppression, viral induced gene silencing [VIGS], post-transcriptional induced gene silencing [PTGS]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8279—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8279—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance
    • C12N15/8282—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance for fungal resistance
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8279—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance
    • C12N15/8285—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance for nematode resistance
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8279—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance
    • C12N15/8286—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance for insect resistance
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/14—Type of nucleic acid interfering nucleic acids [NA]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/16—Aptamers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/30—Chemical structure
    • C12N2310/35—Nature of the modification
    • C12N2310/351—Conjugate
    • C12N2310/3519—Fusion with another nucleic acid
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N2320/00—Applications; Uses
    • C12N2320/30—Special therapeutic applications
    • C12N2320/32—Special delivery means, e.g. tissue-specific
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
    • Y02A40/10—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
    • Y02A40/146—Genetically Modified [GMO] plants, e.g. transgenic plants

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  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Cell Biology (AREA)
  • Pest Control & Pesticides (AREA)
  • Insects & Arthropods (AREA)
  • Virology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)

Abstract

L'invention concerne des procédés et des produits de synthèse permettant d'administrer un ARN en double brin (RNAdb) à des organismes de parasites. Plus précisément, l'invention concerne une molécule d'ARN multidomaine comprenant une séquence nucléotidique renfermant : (i) au moins un aptamère et (ii) au moins une séquence nucléotidique étudiée formant un ARN en brin double, celui-ci comprenant des brins complémentaires recuits, un de ceux-ci présentant une séquence nucléotidique complémentaire d'au moins partiellement une séquence nucléotidique cible de parasites. L'invention concerne également des acides nucléiques codant les molécules d'ARN multidomaine et diverses utilisations des molécules d'ARN multidomaine en agriculture.
PCT/EP2005/011439 2004-10-25 2005-10-25 Molecules destinees a l'administration d'arn en brin double a des organismes de parasites Ceased WO2006045590A2 (fr)

Priority Applications (5)

Application Number Priority Date Filing Date Title
EP05801497A EP1805308A2 (fr) 2004-10-25 2005-10-25 Arn à domaines multiples comprenant au moins un aptamère pour délivrer arn à double brin dans un organisme parasite
MX2007004686A MX2007004686A (es) 2004-10-25 2005-10-25 Moleculas de arn de multidominio que comprenden al menos un aptamero para suministrar arn de doble hebra a organismos nocivos.
AU2005298827A AU2005298827B2 (en) 2004-10-25 2005-10-25 Multidomain RNA molecules comprising at least one aptamer for delivering double stranded RNA to pest organisms
BRPI0516329-3A BRPI0516329A (pt) 2004-10-25 2005-10-25 moléculas de rna de multidomìnio que compreendem pelo menos um aptámero para a aplicação de rna de cordão duplo a organismos de pestes
CA002583334A CA2583334A1 (fr) 2004-10-25 2005-10-25 Molecules destinees a l'administration d'arn en brin double a des organismes de parasites

Applications Claiming Priority (8)

Application Number Priority Date Filing Date Title
US62180104P 2004-10-25 2004-10-25
EP04447235.5 2004-10-25
US60/621,801 2004-10-25
EP04447235 2004-10-25
US62902704P 2004-11-18 2004-11-18
EP04447251.2 2004-11-18
US60/629,027 2004-11-18
EP04447251 2004-11-18

Publications (2)

Publication Number Publication Date
WO2006045590A2 WO2006045590A2 (fr) 2006-05-04
WO2006045590A3 true WO2006045590A3 (fr) 2006-06-15

Family

ID=56290744

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/EP2005/011439 Ceased WO2006045590A2 (fr) 2004-10-25 2005-10-25 Molecules destinees a l'administration d'arn en brin double a des organismes de parasites

Country Status (6)

Country Link
EP (1) EP1805308A2 (fr)
AU (1) AU2005298827B2 (fr)
BR (1) BRPI0516329A (fr)
CA (1) CA2583334A1 (fr)
MX (1) MX2007004686A (fr)
WO (1) WO2006045590A2 (fr)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8946510B2 (en) 2004-04-09 2015-02-03 Monsanto Technology Llc Compositions and methods for control of insect infestations in plants

Families Citing this family (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7612194B2 (en) 2001-07-24 2009-11-03 Monsanto Technology Llc Nucleic acid sequences from Diabrotica virgifera virgifera LeConte and uses thereof
US20050256071A1 (en) * 2003-07-15 2005-11-17 California Institute Of Technology Inhibitor nucleic acids
EP3508582B1 (fr) 2005-09-16 2021-01-13 Monsanto Technology LLC Procédés de contrôle génétique d'infestation d'insectes dans des plantes et compositions correspondantes
WO2007083193A2 (fr) 2005-09-16 2007-07-26 Devgen Nv Procédé de protection phytosanitaire à base d'interférence arn
KR20090014352A (ko) * 2006-06-01 2009-02-10 듀크 유니버시티 전달 방법
US8809625B2 (en) 2008-01-17 2014-08-19 Pioneer Hi-Bred International, Inc. Compositions and methods for the suppression of target polynucleotides from Lygus
US8847013B2 (en) 2008-01-17 2014-09-30 Pioneer Hi Bred International Inc Compositions and methods for the suppression of target polynucleotides from lepidoptera
US8367895B2 (en) * 2008-01-17 2013-02-05 Pioneer Hi-Bred International, Inc. Compositions and methods for the suppression of target polynucleotides from the family aphididae
US8872001B2 (en) 2010-06-04 2014-10-28 Pioneer Hi Bred International Inc Compositions and methods for insecticidal control of stinkbugs
US10676743B2 (en) 2013-12-20 2020-06-09 The University Of Queensland Plant-protecting RNAi compositions comprising plant-protecting double-stranded RNA adsorbed onto layered double hydroxide particles
MY189214A (en) 2015-06-19 2022-01-31 Univ Queensland Composition
CN112899301B (zh) * 2021-01-29 2023-04-11 中国热带农业科学院热带生物技术研究所 一种木薯普通花叶病毒诱导的基因沉默系统及其应用

Non-Patent Citations (5)

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Title
DIECKMANN THORSTEN ET AL: "Solution structure of an ATP-binding RNA aptamer reveals a novel fold", RNA (NEW YORK), vol. 2, no. 7, 1996, pages 628 - 640, XP002325933, ISSN: 1355-8382 *
FAROKHZAD OMID C ET AL: "Nanoparticle-aptamer bioconjugates: a new approach for targeting prostate cancer cells.", CANCER RESEARCH. 1 NOV 2004, vol. 64, no. 21, 1 November 2004 (2004-11-01), pages 7668 - 7672, XP002325935, ISSN: 0008-5472 *
LATO SUSAN M ET AL: "Boron-containing aptamers to ATP.", NUCLEIC ACIDS RESEARCH. 15 MAR 2002, vol. 30, no. 6, 15 March 2002 (2002-03-15), pages 1401 - 1407, XP002325934, ISSN: 1362-4962 *
NISHIKAWA F ET AL: "Inhibition of HCV protease by RNA aptamers in cells", NUCLEIC ACIDS RESEARCH, OXFORD UNIVERSITY PRESS, SURREY, GB, vol. 31, no. 7, 1 April 2003 (2003-04-01), pages 1935 - 1943, XP002260526, ISSN: 0305-1048 *
SCHERR M ET AL: "GENE SILENCING MEDIATED BY SMALL INTERFERING RNAS IN MAMMALIAN CELLS", CURRENT MEDICINAL CHEMISTRY, BENTHAM SCIENCE PUBLISHERS BV, BE, vol. 10, no. 3, February 2003 (2003-02-01), pages 245 - 256, XP009019025, ISSN: 0929-8673 *

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8946510B2 (en) 2004-04-09 2015-02-03 Monsanto Technology Llc Compositions and methods for control of insect infestations in plants
US9238822B2 (en) 2004-04-09 2016-01-19 Monsanto Technology Llc Compositions and methods for control of insect infestations in plants
US9340797B2 (en) 2004-04-09 2016-05-17 Monsanto Technology Llc Compositions and methods for control of insect infestations in plants

Also Published As

Publication number Publication date
AU2005298827B2 (en) 2011-09-08
WO2006045590A2 (fr) 2006-05-04
CA2583334A1 (fr) 2006-05-04
AU2005298827A1 (en) 2006-05-04
BRPI0516329A (pt) 2008-03-11
EP1805308A2 (fr) 2007-07-11
MX2007004686A (es) 2007-06-14

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