[go: up one dir, main page]

WO2005003778A3 - A method for identifying a synthetic molecule having affinity towards a target - Google Patents

A method for identifying a synthetic molecule having affinity towards a target Download PDF

Info

Publication number
WO2005003778A3
WO2005003778A3 PCT/DK2004/000466 DK2004000466W WO2005003778A3 WO 2005003778 A3 WO2005003778 A3 WO 2005003778A3 DK 2004000466 W DK2004000466 W DK 2004000466W WO 2005003778 A3 WO2005003778 A3 WO 2005003778A3
Authority
WO
WIPO (PCT)
Prior art keywords
target
affinity towards
identifying
library
molecule
Prior art date
Application number
PCT/DK2004/000466
Other languages
French (fr)
Other versions
WO2005003778A2 (en
Inventor
Per-Ola Freskgaard
Mikkel Dybro Lundorf
Soeren Nyboe Jakobsen
Original Assignee
Nuevolution As
Per-Ola Freskgaard
Mikkel Dybro Lundorf
Soeren Nyboe Jakobsen
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Nuevolution As, Per-Ola Freskgaard, Mikkel Dybro Lundorf, Soeren Nyboe Jakobsen filed Critical Nuevolution As
Publication of WO2005003778A2 publication Critical patent/WO2005003778A2/en
Publication of WO2005003778A3 publication Critical patent/WO2005003778A3/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C40—COMBINATORIAL TECHNOLOGY
    • C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B30/00—Methods of screening libraries
    • C40B30/04—Methods of screening libraries by measuring the ability to specifically bind a target molecule, e.g. antibody-antigen binding, receptor-ligand binding
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1068—Template (nucleic acid) mediated chemical library synthesis, e.g. chemical and enzymatical DNA-templated organic molecule synthesis, libraries prepared by non ribosomal polypeptide synthesis [NRPS], DNA/RNA-polymerase mediated polypeptide synthesis

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Wood Science & Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Plant Pathology (AREA)
  • Medicinal Chemistry (AREA)
  • Microbiology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Immunology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Organic Low-Molecular-Weight Compounds And Preparation Thereof (AREA)

Abstract

The present invention relates to a method for identifying a synthetic molecule having affinity towards a target. The method includes the steps of providing a library of bifunctional complexes, wherein each complex of the library comprises a synthetic molecule attached to an identifier, which codes for said molecule; subjecting, under binding conditions, the library of bifunctional complexes to a target; removing the non-binding members of the library; separating the identifiers of complexes comprising synthetic molecules having affinity towards the target, and decoding the identifiers to establish the identity of the molecule.
PCT/DK2004/000466 2003-07-02 2004-06-30 A method for identifying a synthetic molecule having affinity towards a target WO2005003778A2 (en)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US48389903P 2003-07-02 2003-07-02
DKPA200301003 2003-07-02
US60/483,899 2003-07-02
DKPA200301003 2003-07-02

Publications (2)

Publication Number Publication Date
WO2005003778A2 WO2005003778A2 (en) 2005-01-13
WO2005003778A3 true WO2005003778A3 (en) 2005-04-14

Family

ID=33566213

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/DK2004/000466 WO2005003778A2 (en) 2003-07-02 2004-06-30 A method for identifying a synthetic molecule having affinity towards a target

Country Status (1)

Country Link
WO (1) WO2005003778A2 (en)

Cited By (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7223545B2 (en) 2001-03-19 2007-05-29 President And Fellows Of Harvard College Evolving new molecular function
US8206901B2 (en) 2002-10-30 2012-06-26 Nuevolution A/S Method for the synthesis of a bifunctional complex
US8808984B2 (en) 2002-03-15 2014-08-19 Neuvolution A/S Method for synthesising templated molecules
US8932992B2 (en) 2001-06-20 2015-01-13 Nuevolution A/S Templated molecules and methods for using such molecules
US9096951B2 (en) 2003-02-21 2015-08-04 Nuevolution A/S Method for producing second-generation library
US9121110B2 (en) 2002-12-19 2015-09-01 Nuevolution A/S Quasirandom structure and function guided synthesis methods
US9574189B2 (en) 2005-12-01 2017-02-21 Nuevolution A/S Enzymatic encoding methods for efficient synthesis of large libraries

Families Citing this family (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1539980B1 (en) 2002-08-01 2016-02-17 Nuevolution A/S Library of complexes comprising small non-peptide molecules and double-stranded oligonucleotides identifying the molecules
US7915201B2 (en) 2003-03-20 2011-03-29 Nuevolution A/S Ligational encoding of small molecules
US11118215B2 (en) 2003-09-18 2021-09-14 Nuevolution A/S Method for obtaining structural information concerning an encoded molecule and method for selecting compounds
US7972994B2 (en) 2003-12-17 2011-07-05 Glaxosmithkline Llc Methods for synthesis of encoded libraries
NZ547723A (en) 2003-12-17 2009-09-25 Praecis Pharm Inc Methods for synthesis of encoded libraries
ATE447020T1 (en) 2004-03-22 2009-11-15 Nuevolution As LIGATION CODING USING OLIGONUCLEOTIDE BUILDING BLOCKS
US7422855B2 (en) 2004-06-10 2008-09-09 Perkinelmer Las, Inc. Multiplexing assays for analyte detection
EP1940755A2 (en) 2005-10-28 2008-07-09 Praecis Pharmaceuticals Inc. Methods for identifying compounds of interest using encoded libraries
DK2558577T3 (en) 2010-04-16 2019-04-01 Nuevolution As Bi-functional complexes and methods for the preparation and use of such complexes
EP3218479A1 (en) 2014-11-11 2017-09-20 Nanocore ApS Method for identification of molecules with desired characteristics
JP7283727B2 (en) 2016-06-16 2023-05-30 ヘイスタック サイエンシィズ コーポレーション Oligonucleotides Directed and Recorded for Combinatorial Synthesis of Code Probe Molecules
EP3619340A4 (en) 2017-05-02 2021-01-20 Haystack Sciences Corporation MOLECULES FOR THE VERIFICATION OF OLIGONUCLEOTIDE DIRECTED COMBINATIONAL SYNTHESIS AND METHOD OF MANUFACTURING AND USING THEREOF

Citations (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1993006121A1 (en) * 1991-09-18 1993-04-01 Affymax Technologies N.V. Method of synthesizing diverse collections of oligomers
WO1995004160A1 (en) * 1993-07-30 1995-02-09 Isis Innovation Limited Tag reagent and assay method
WO1995028640A1 (en) * 1994-04-13 1995-10-26 The Trustees Of Columbia University In The City Of New York Complex combinatorial chemical libraries encoded with tags
US5573905A (en) * 1992-03-30 1996-11-12 The Scripps Research Institute Encoded combinatorial chemical libraries
WO1999060007A2 (en) * 1998-05-15 1999-11-25 Isis Innovation Limited Libraries of oligomers labelled with different tags
US6027890A (en) * 1996-01-23 2000-02-22 Rapigene, Inc. Methods and compositions for enhancing sensitivity in the analysis of biological-based assays
WO2002074929A2 (en) * 2001-03-19 2002-09-26 President And Fellows Of Harvard College Evolving new molecular function
WO2003002767A1 (en) * 2001-06-29 2003-01-09 Agilent Technologies, Inc. Method of dna sequencing using cleavable tags

Patent Citations (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1993006121A1 (en) * 1991-09-18 1993-04-01 Affymax Technologies N.V. Method of synthesizing diverse collections of oligomers
US5573905A (en) * 1992-03-30 1996-11-12 The Scripps Research Institute Encoded combinatorial chemical libraries
WO1995004160A1 (en) * 1993-07-30 1995-02-09 Isis Innovation Limited Tag reagent and assay method
WO1995028640A1 (en) * 1994-04-13 1995-10-26 The Trustees Of Columbia University In The City Of New York Complex combinatorial chemical libraries encoded with tags
US6027890A (en) * 1996-01-23 2000-02-22 Rapigene, Inc. Methods and compositions for enhancing sensitivity in the analysis of biological-based assays
WO1999060007A2 (en) * 1998-05-15 1999-11-25 Isis Innovation Limited Libraries of oligomers labelled with different tags
WO2002074929A2 (en) * 2001-03-19 2002-09-26 President And Fellows Of Harvard College Evolving new molecular function
WO2003002767A1 (en) * 2001-06-29 2003-01-09 Agilent Technologies, Inc. Method of dna sequencing using cleavable tags

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
BRENNER S ET AL: "ENCODED COMBINATORIAL CHEMISTRY", PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF USA, NATIONAL ACADEMY OF SCIENCE. WASHINGTON, US, vol. 89, no. 12, 1 June 1992 (1992-06-01), pages 5381 - 5383, XP000647936, ISSN: 0027-8424 *

Cited By (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7223545B2 (en) 2001-03-19 2007-05-29 President And Fellows Of Harvard College Evolving new molecular function
US7442160B2 (en) 2001-03-19 2008-10-28 President And Fellows Of Harvard College Evolving new molecular function
US7557068B2 (en) 2001-03-19 2009-07-07 President And Fellows Of Harvard College Evolving new molecular function
US8932992B2 (en) 2001-06-20 2015-01-13 Nuevolution A/S Templated molecules and methods for using such molecules
US8808984B2 (en) 2002-03-15 2014-08-19 Neuvolution A/S Method for synthesising templated molecules
US8206901B2 (en) 2002-10-30 2012-06-26 Nuevolution A/S Method for the synthesis of a bifunctional complex
US8722583B2 (en) 2002-10-30 2014-05-13 Nuevolution A/S Method for selecting a chemical entity from a tagged library
US9109248B2 (en) 2002-10-30 2015-08-18 Nuevolution A/S Method for the synthesis of a bifunctional complex
US9284600B2 (en) 2002-10-30 2016-03-15 Neuvolution A/S Method for the synthesis of a bifunctional complex
US9121110B2 (en) 2002-12-19 2015-09-01 Nuevolution A/S Quasirandom structure and function guided synthesis methods
US9096951B2 (en) 2003-02-21 2015-08-04 Nuevolution A/S Method for producing second-generation library
US9574189B2 (en) 2005-12-01 2017-02-21 Nuevolution A/S Enzymatic encoding methods for efficient synthesis of large libraries

Also Published As

Publication number Publication date
WO2005003778A2 (en) 2005-01-13

Similar Documents

Publication Publication Date Title
WO2005003778A3 (en) A method for identifying a synthetic molecule having affinity towards a target
WO2005023986A3 (en) Microrna as ligands and target molecules
WO2003061570A3 (en) Engineered binding proteins
WO2007038523A3 (en) Method of isolating analytes from a sample
EP0795026A4 (en) RIBOZYMS AND NEW SELECTION SYSTEM
AU2001257012A1 (en) Methods for selective targeting
WO2003087129A3 (en) Immunogenic peptides, and method of identifying same
ATE552274T1 (en) MHC OLIGOMER AND METHOD FOR THE PRODUCTION THEREOF
WO1999015653A3 (en) Tie ligand homologues
EA200702210A1 (en) METHOD FOR CLEANING PROTEINS
WO2003092581A3 (en) Capture compounds, collections thereof and methods for analyzing the proteome and complex compositions
ATE307649T1 (en) CONTINUOUS PROCESS FOR SEPARATING SUBSTANCES BY MOLECULE SIZE
ATE350154T1 (en) METHOD FOR PRODUCING POROUS INORGANIC/ORGANIC HYBRID PARTICLES FOR CHROMATOGRAPHIC SEPARATION
WO2004001418A3 (en) Method for identifying and extracting endotoxin
IL199409A (en) Method for isolating an intestinal cholesterol binding protein, intestinal cholesterol binding protein, its complexes and uses thereof
WO2006107288A3 (en) Magnetic field enhanced cake-filtration solid-liquid separations
WO1998016661A3 (en) Morphatides: novel shape and structure libraries
WO2003002724A3 (en) Proteins, druggable regions of proteins and target analysis for chemistry of therapeutics
WO2001051652A3 (en) Method for identifying a mark applied on a solid body
WO2002029094A3 (en) Method of selection, by two-dimensional separation, of nucleic acids that bind to a target with high affinity
WO2002057792A3 (en) Affinity selection-based screening of hydrophobic proteins
WO2004031724A3 (en) Apparatus and method for expression and capture of biomolecules and complexes on adsorbent surfaces
WO2004101620A3 (en) Serum albumin scaffold-based proteins and uses thereof
WO2003091415A3 (en) Fluorobodies and chromobodies: binding ligands with intrinsic fluorescence and color
ATE380344T1 (en) METHOD FOR IDENTIFYING DRUG TARGETS

Legal Events

Date Code Title Description
AK Designated states

Kind code of ref document: A2

Designated state(s): AE AG AL AM AT AU AZ BA BB BG BR BW BY BZ CA CH CN CO CR CU CZ DE DK DM DZ EC EE EG ES FI GB GD GE GH GM HR HU ID IL IN IS JP KE KG KP KR KZ LC LK LR LS LT LU LV MA MD MG MK MN MW MX MZ NA NI NO NZ OM PG PH PL PT RO RU SC SD SE SG SK SL SY TJ TM TN TR TT TZ UA UG US UZ VC VN YU ZA ZM ZW

AL Designated countries for regional patents

Kind code of ref document: A2

Designated state(s): BW GH GM KE LS MW MZ NA SD SL SZ TZ UG ZM ZW AM AZ BY KG KZ MD RU TJ TM AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HU IE IT LU MC NL PL PT RO SE SI SK TR BF BJ CF CG CI CM GA GN GQ GW ML MR NE SN TD TG

121 Ep: the epo has been informed by wipo that ep was designated in this application
122 Ep: pct application non-entry in european phase