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WO2004038367A3 - Biological assays using gradients formed in microfluidic systems - Google Patents

Biological assays using gradients formed in microfluidic systems Download PDF

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Publication number
WO2004038367A3
WO2004038367A3 PCT/US2003/033146 US0333146W WO2004038367A3 WO 2004038367 A3 WO2004038367 A3 WO 2004038367A3 US 0333146 W US0333146 W US 0333146W WO 2004038367 A3 WO2004038367 A3 WO 2004038367A3
Authority
WO
WIPO (PCT)
Prior art keywords
biological assays
microfluidic systems
present
gradients formed
gradients
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/US2003/033146
Other languages
French (fr)
Other versions
WO2004038367A2 (en
Inventor
Gregory L Kirk
Enoch Kim
Emanuele Ostuni
Olivier Schueller
Paul Sweetnam
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Surface Logix Inc
Original Assignee
Surface Logix Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Surface Logix Inc filed Critical Surface Logix Inc
Priority to AU2003287170A priority Critical patent/AU2003287170A1/en
Priority to EP03781348A priority patent/EP1578194A4/en
Priority to JP2004546909A priority patent/JP2006507815A/en
Priority to CA002503186A priority patent/CA2503186A1/en
Publication of WO2004038367A2 publication Critical patent/WO2004038367A2/en
Publication of WO2004038367A3 publication Critical patent/WO2004038367A3/en
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • G01N33/5008—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
    • G01N33/502—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics for testing non-proliferative effects
    • G01N33/5029—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics for testing non-proliferative effects on cell motility
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5025—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures for parallel transport of multiple samples
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B82—NANOTECHNOLOGY
    • B82Y—SPECIFIC USES OR APPLICATIONS OF NANOSTRUCTURES; MEASUREMENT OR ANALYSIS OF NANOSTRUCTURES; MANUFACTURE OR TREATMENT OF NANOSTRUCTURES
    • B82Y15/00—Nanotechnology for interacting, sensing or actuating, e.g. quantum dots as markers in protein assays or molecular motors
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B82—NANOTECHNOLOGY
    • B82Y—SPECIFIC USES OR APPLICATIONS OF NANOSTRUCTURES; MEASUREMENT OR ANALYSIS OF NANOSTRUCTURES; MANUFACTURE OR TREATMENT OF NANOSTRUCTURES
    • B82Y30/00—Nanotechnology for materials or surface science, e.g. nanocomposites
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/06—Auxiliary integrated devices, integrated components
    • B01L2300/0681—Filter
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0809—Geometry, shape and general structure rectangular shaped
    • B01L2300/0829—Multi-well plates; Microtitration plates
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Nanotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Hematology (AREA)
  • Immunology (AREA)
  • Biomedical Technology (AREA)
  • Analytical Chemistry (AREA)
  • Cell Biology (AREA)
  • Physics & Mathematics (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Urology & Nephrology (AREA)
  • General Physics & Mathematics (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Biochemistry (AREA)
  • Materials Engineering (AREA)
  • Microbiology (AREA)
  • Condensed Matter Physics & Semiconductors (AREA)
  • Toxicology (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Composite Materials (AREA)
  • Pathology (AREA)
  • Clinical Laboratory Science (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Investigating Or Analysing Biological Materials (AREA)

Abstract

The present invention discloses a device for monitoring chemotaxis or chemoinvasion. The present invention further provides a flexible assay system and numerous assays that can be used to test biological interactions and systems. Laminar flow gradients are employed that mimic gradient situations present in vivo.
PCT/US2003/033146 2002-10-22 2003-10-21 Biological assays using gradients formed in microfluidic systems Ceased WO2004038367A2 (en)

Priority Applications (4)

Application Number Priority Date Filing Date Title
AU2003287170A AU2003287170A1 (en) 2002-10-22 2003-10-21 Biological assays using gradients formed in microfluidic systems
EP03781348A EP1578194A4 (en) 2002-10-22 2003-10-21 Biological assays using gradients formed in microfluidic systems
JP2004546909A JP2006507815A (en) 2002-10-22 2003-10-21 Biological assays using gradients formed in microfluidic systems
CA002503186A CA2503186A1 (en) 2002-10-22 2003-10-21 Biological assays using gradients formed in microfluidic systems

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US41997602P 2002-10-22 2002-10-22
US41998002P 2002-10-22 2002-10-22
US60/419,976 2002-10-22
US60/419,980 2002-10-22

Publications (2)

Publication Number Publication Date
WO2004038367A2 WO2004038367A2 (en) 2004-05-06
WO2004038367A3 true WO2004038367A3 (en) 2004-11-11

Family

ID=32179774

Family Applications (2)

Application Number Title Priority Date Filing Date
PCT/US2003/033177 Ceased WO2004038368A2 (en) 2002-10-22 2003-10-21 Device and method for monitoring leukocyte migration
PCT/US2003/033146 Ceased WO2004038367A2 (en) 2002-10-22 2003-10-21 Biological assays using gradients formed in microfluidic systems

Family Applications Before (1)

Application Number Title Priority Date Filing Date
PCT/US2003/033177 Ceased WO2004038368A2 (en) 2002-10-22 2003-10-21 Device and method for monitoring leukocyte migration

Country Status (5)

Country Link
EP (2) EP1578194A4 (en)
JP (2) JP2006503581A (en)
AU (2) AU2003282950A1 (en)
CA (2) CA2503203A1 (en)
WO (2) WO2004038368A2 (en)

Families Citing this family (19)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101061213B (en) * 2004-05-19 2012-12-19 麻省理工学院 Perfused three-dimensional cell/tissue disease models
US7351423B2 (en) 2004-09-01 2008-04-01 Depuy Spine, Inc. Musculo-skeletal implant having a bioactive gradient
KR100873383B1 (en) 2007-11-13 2008-12-10 이화여자대학교 산학협력단 Microfluidic device for the study of chemotaxis of nematodes
JP5602718B2 (en) * 2008-04-08 2014-10-08 マサチューセッツ インスティテュート オブ テクノロジー Three-dimensional microfluidic platform and method of use
DE102009035502A1 (en) 2009-07-30 2011-02-03 Universitätsklinikum Jena Method and device for detecting the movement and attachment of cells and particles to cell, tissue and implant layers in the simulation of flow conditions
DE102009038520B4 (en) * 2009-08-25 2012-07-05 Universität Rostock Method and device for cell sample diagnostics
CN102580798B (en) * 2012-02-28 2014-03-05 东北大学 Solution gradient dilution device with microfluidic chip
JP6385339B2 (en) * 2012-04-18 2018-09-05 ヘモシアー・リミテッド・ライアビリティ・カンパニーＨｅｍｏＳｈｅａｒ， ＬＬＣ In vitro model for pathological or physiological conditions
JP5910282B2 (en) * 2012-04-20 2016-04-27 大日本印刷株式会社 Cell migration analysis method and cell culture preparation method
KR101433091B1 (en) * 2012-04-26 2014-08-25 한국과학기술원 Chematoxis Analysis Microfluidic Apparatus of bacteria, Production Method and Chematoxis Analysis Method of bacteria
JP6684706B2 (en) 2013-10-21 2020-04-22 ヘモシアー・リミテッド・ライアビリティ・カンパニーＨｅｍｏＳｈｅａｒ， ＬＬＣ In vitro model for tumor microenvironment
WO2016065125A1 (en) * 2014-10-22 2016-04-28 The General Hospital Corporation Method and apparatus for visualizing objects using oblique back-illumination
CN105080627A (en) * 2015-08-25 2015-11-25 辽宁中医药大学 Integrated microfluidic chip for screening medicine and method for applying integrated microfluidic chip
CN106497788A (en) * 2016-11-28 2017-03-15 南方医科大学珠江医院 Hepatocyte heterogeneity culture apparatuses and its cultural method
WO2019045926A1 (en) * 2017-08-30 2019-03-07 Imprimed, Inc. Devices and methods for high-throughput screening of chemical and biochemical compounds
CN111019828B (en) * 2019-12-26 2021-12-21 武汉大学 Open type high-flux microfluidic oocyte dynamic three-dimensional culture chip and application thereof
US20230364613A1 (en) * 2020-09-29 2023-11-16 Nok Corporation Leukocyte trapping apparatus
EP4469798A1 (en) 2022-01-28 2024-12-04 GlaxoSmithKline Intellectual Property Development Limited High-throughput assay for cell migration, chemotaxis, and function
CN114887034B (en) * 2022-07-14 2022-10-28 中山莱博瑞辰生物医药有限公司 Use of LLP2A-Ale for the preparation of a medicament for the treatment and/or prevention of peripheral blood lymphopenia

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1998052691A1 (en) * 1997-05-16 1998-11-26 Alberta Research Council Microfluidic system and methods of use
WO2000073799A1 (en) * 1999-06-01 2000-12-07 Caliper Technologies Corp. Microscale assays and microfluidic devices for transporter, gradient induced, and binding activities
US20020168757A1 (en) * 2000-11-08 2002-11-14 Gregory Kirk Test device and method of making same

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5284753A (en) * 1991-03-20 1994-02-08 Neuro Probe, Inc. Multiple-site chemotactic test apparatus and method
US5460945A (en) * 1991-05-30 1995-10-24 Center For Blood Research, Inc. Device and method for analysis of blood components and identifying inhibitors and promoters of the inflammatory response
US6632619B1 (en) * 1997-05-16 2003-10-14 The Governors Of The University Of Alberta Microfluidic system and methods of use
US7326563B2 (en) * 2000-11-08 2008-02-05 Surface Logix, Inc. Device and method for monitoring leukocyte migration
AU2003213834A1 (en) * 2002-03-12 2003-09-29 Surface Logix, Inc. Cell motility and chemotaxis test device and methods of using same

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1998052691A1 (en) * 1997-05-16 1998-11-26 Alberta Research Council Microfluidic system and methods of use
WO2000073799A1 (en) * 1999-06-01 2000-12-07 Caliper Technologies Corp. Microscale assays and microfluidic devices for transporter, gradient induced, and binding activities
US20020168757A1 (en) * 2000-11-08 2002-11-14 Gregory Kirk Test device and method of making same

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
JEON ET AL: "Neutrophil chemotaxis in linear and complex gradients of interleukin-8 formed ina microfabricated device", NATURE BIOTECH, vol. 20, 1 July 2002 (2002-07-01), pages 826 - 830, XP002978726 *
See also references of EP1578194A4 *

Also Published As

Publication number Publication date
EP1558076A2 (en) 2005-08-03
EP1578194A4 (en) 2007-03-21
AU2003287170A1 (en) 2004-05-13
CA2503186A1 (en) 2004-05-06
EP1558076A4 (en) 2007-03-21
JP2006507815A (en) 2006-03-09
EP1578194A2 (en) 2005-09-28
WO2004038368A2 (en) 2004-05-06
WO2004038367A2 (en) 2004-05-06
CA2503203A1 (en) 2004-05-06
WO2004038368A3 (en) 2004-07-29
AU2003282950A1 (en) 2004-05-13
JP2006503581A (en) 2006-02-02

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