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WO2004025265A3 - Test strip and method for determining ldl cholesterol concentration from whole blood - Google Patents

Test strip and method for determining ldl cholesterol concentration from whole blood Download PDF

Info

Publication number
WO2004025265A3
WO2004025265A3 PCT/US2003/029102 US0329102W WO2004025265A3 WO 2004025265 A3 WO2004025265 A3 WO 2004025265A3 US 0329102 W US0329102 W US 0329102W WO 2004025265 A3 WO2004025265 A3 WO 2004025265A3
Authority
WO
WIPO (PCT)
Prior art keywords
concentration
test strip
ldl cholesterol
whole blood
panel
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/US2003/029102
Other languages
French (fr)
Other versions
WO2004025265A2 (en
Inventor
Bruce Shull
Hyeon-Sook Lee Zeng
Sunil Anaokar
Gena Lynn Antonopoulos
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Polymer Technology Systems Inc
Original Assignee
Polymer Technology Systems Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Polymer Technology Systems Inc filed Critical Polymer Technology Systems Inc
Priority to CA002499058A priority Critical patent/CA2499058A1/en
Priority to AU2003272453A priority patent/AU2003272453A1/en
Priority to MXPA05002955A priority patent/MXPA05002955A/en
Priority to EP03754634A priority patent/EP1540353A4/en
Publication of WO2004025265A2 publication Critical patent/WO2004025265A2/en
Publication of WO2004025265A3 publication Critical patent/WO2004025265A3/en
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/52—Use of compounds or compositions for colorimetric, spectrophotometric or fluorometric investigation, e.g. use of reagent paper and including single- and multilayer analytical elements
    • G01N33/525—Multi-layer analytical elements
    • G01N33/526—Multi-layer analytical elements the element being adapted for a specific analyte
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/92—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving lipids, e.g. cholesterol, lipoproteins, or their receptors
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2800/00—Detection or diagnosis of diseases
    • G01N2800/04—Endocrine or metabolic disorders
    • G01N2800/044—Hyperlipemia or hypolipemia, e.g. dyslipidaemia, obesity

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Hematology (AREA)
  • Immunology (AREA)
  • Molecular Biology (AREA)
  • Urology & Nephrology (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • Pathology (AREA)
  • Cell Biology (AREA)
  • Food Science & Technology (AREA)
  • Biotechnology (AREA)
  • General Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Endocrinology (AREA)
  • Biophysics (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

A dry phase test strip (20) and method are provided for determining the concentration of LDL in whole blood or plasma. The inventive test strip (20) includes one stack (92) or panel that measures concentration of total cholesterol and another stack (94) or panel that measures concentration of the sum total of HDL, VLDL and chylomicrons ('non-LDLs'). The difference between the values just noted is equal to the concentration of LDL cholesterol. Dry phase test strips (20) of the present invention function at room temperature and all test results are produced from pseudo-endpoint reflectance measurements such that the test method need not be timed. Also disclosed is the capability for an improved lipid panel that provides concentration in a blood sample of HDL, total cholesterol and LDL cholesterol without relying upon the Friedewald equation.
PCT/US2003/029102 2002-09-16 2003-09-16 Test strip and method for determining ldl cholesterol concentration from whole blood Ceased WO2004025265A2 (en)

Priority Applications (4)

Application Number Priority Date Filing Date Title
CA002499058A CA2499058A1 (en) 2002-09-16 2003-09-16 Test strip and method for determining ldl cholesterol concentration from whole blood
AU2003272453A AU2003272453A1 (en) 2002-09-16 2003-09-16 Test strip and method for determining ldl cholesterol concentration from whole blood
MXPA05002955A MXPA05002955A (en) 2002-09-16 2003-09-16 Test strip and method for determining ldl cholesterol concentration from whole blood.
EP03754634A EP1540353A4 (en) 2002-09-16 2003-09-16 Test strip and method for determining ldl cholesterol concentration from whole blood

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US41120902P 2002-09-16 2002-09-16
US60/411,209 2002-09-16

Publications (2)

Publication Number Publication Date
WO2004025265A2 WO2004025265A2 (en) 2004-03-25
WO2004025265A3 true WO2004025265A3 (en) 2004-07-01

Family

ID=31994248

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2003/029102 Ceased WO2004025265A2 (en) 2002-09-16 2003-09-16 Test strip and method for determining ldl cholesterol concentration from whole blood

Country Status (6)

Country Link
EP (1) EP1540353A4 (en)
AU (1) AU2003272453A1 (en)
CA (1) CA2499058A1 (en)
MX (1) MXPA05002955A (en)
WO (1) WO2004025265A2 (en)
ZA (1) ZA200502214B (en)

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7582484B2 (en) 2002-01-18 2009-09-01 Cholestech Corporation High-density lipoprotein assay device and method
US7772007B2 (en) 2004-04-02 2010-08-10 Cholestech Corporation Assay device for direct measurement of LDL cholesterol
US7795038B2 (en) 2002-04-09 2010-09-14 Cholestech Corporation High-density lipoprotein assay device and method
US7824879B2 (en) 2007-01-09 2010-11-02 Cholestech Corporation Device and method for measuring LDL-associated cholesterol

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8304204B2 (en) 2007-09-05 2012-11-06 Arkray, Inc. Method for measuring low-density lipoprotein (LDL) cholesterol

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5132086A (en) * 1990-02-06 1992-07-21 Chemtrak Corporation Non-instrumented cholesterol assay
US5135716A (en) * 1989-07-12 1992-08-04 Kingston Diagnostics, L.P. Direct measurement of HDL cholesterol via dry chemistry strips
US5460974A (en) * 1992-10-13 1995-10-24 Miles Inc. Method of assaying whole blood for HDL cholesterol

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP3193634B2 (en) * 1996-05-29 2001-07-30 第一化学薬品株式会社 LDL cholesterol determination method
JP3441993B2 (en) * 1999-01-27 2003-09-02 松下電器産業株式会社 Cholesterol sensor
US6524864B2 (en) * 2000-12-28 2003-02-25 Aurora L. Fernandez Decastro Test strip for simultaneous detection of a plurality of analytes

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5135716A (en) * 1989-07-12 1992-08-04 Kingston Diagnostics, L.P. Direct measurement of HDL cholesterol via dry chemistry strips
US5132086A (en) * 1990-02-06 1992-07-21 Chemtrak Corporation Non-instrumented cholesterol assay
US5460974A (en) * 1992-10-13 1995-10-24 Miles Inc. Method of assaying whole blood for HDL cholesterol

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
See also references of EP1540353A4 *

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7582484B2 (en) 2002-01-18 2009-09-01 Cholestech Corporation High-density lipoprotein assay device and method
US7795038B2 (en) 2002-04-09 2010-09-14 Cholestech Corporation High-density lipoprotein assay device and method
US7772007B2 (en) 2004-04-02 2010-08-10 Cholestech Corporation Assay device for direct measurement of LDL cholesterol
US7824879B2 (en) 2007-01-09 2010-11-02 Cholestech Corporation Device and method for measuring LDL-associated cholesterol

Also Published As

Publication number Publication date
WO2004025265A2 (en) 2004-03-25
CA2499058A1 (en) 2004-03-25
ZA200502214B (en) 2006-05-31
MXPA05002955A (en) 2005-10-18
AU2003272453A1 (en) 2004-04-30
EP1540353A4 (en) 2007-01-24
EP1540353A2 (en) 2005-06-15

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