[go: up one dir, main page]

WO2004018998A3 - Method using real-time pcr - Google Patents

Method using real-time pcr Download PDF

Info

Publication number
WO2004018998A3
WO2004018998A3 PCT/US2003/026177 US0326177W WO2004018998A3 WO 2004018998 A3 WO2004018998 A3 WO 2004018998A3 US 0326177 W US0326177 W US 0326177W WO 2004018998 A3 WO2004018998 A3 WO 2004018998A3
Authority
WO
WIPO (PCT)
Prior art keywords
time pcr
real
qpcr
present
quantitative
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/US2003/026177
Other languages
French (fr)
Other versions
WO2004018998A2 (en
Inventor
Daniel C Edelman
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
University of Maryland Baltimore
University of Maryland College Park
Original Assignee
University of Maryland Baltimore
University of Maryland College Park
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by University of Maryland Baltimore, University of Maryland College Park filed Critical University of Maryland Baltimore
Priority to AU2003272230A priority Critical patent/AU2003272230A1/en
Publication of WO2004018998A2 publication Critical patent/WO2004018998A2/en
Publication of WO2004018998A3 publication Critical patent/WO2004018998A3/en
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6851—Quantitative amplification

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Physics & Mathematics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Immunology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Other Investigation Or Analysis Of Materials By Electrical Means (AREA)

Abstract

The present invention demonstrates a method for performing quantitative or qualitative analysis on a crude biological or environmental sample utilizing quantitative real-time PCR (QPCR).
PCT/US2003/026177 2002-08-20 2003-08-20 Method using real-time pcr Ceased WO2004018998A2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU2003272230A AU2003272230A1 (en) 2002-08-20 2003-08-20 Method using real-time pcr

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US40462202P 2002-08-20 2002-08-20
US60/404,622 2002-08-20
US42765002P 2002-11-19 2002-11-19
US60/427,650 2002-11-19

Publications (2)

Publication Number Publication Date
WO2004018998A2 WO2004018998A2 (en) 2004-03-04
WO2004018998A3 true WO2004018998A3 (en) 2004-06-24

Family

ID=31949865

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2003/026177 Ceased WO2004018998A2 (en) 2002-08-20 2003-08-20 Method using real-time pcr

Country Status (2)

Country Link
AU (1) AU2003272230A1 (en)
WO (1) WO2004018998A2 (en)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9464331B2 (en) 2011-07-06 2016-10-11 Quest Diagnostics Investments Incorporated Direct amplification and detection of viral and bacterial pathogens
CN114369683B (en) * 2021-09-16 2023-07-25 中山大学 Detection kit for prawn virus in Malaysia

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6033854A (en) * 1991-12-16 2000-03-07 Biotronics Corporation Quantitative PCR using blocking oligonucleotides
US6447994B1 (en) * 2000-06-20 2002-09-10 The General Hospital Corporation Production of replicative hepatitis C virus

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6033854A (en) * 1991-12-16 2000-03-07 Biotronics Corporation Quantitative PCR using blocking oligonucleotides
US6447994B1 (en) * 2000-06-20 2002-09-10 The General Hospital Corporation Production of replicative hepatitis C virus

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
RAVAGGI ET AL.: "Direct PCR amplification of HCV RNA from human serum", PCR METHODS AND APPLICATIONS, vol. 1, 1992, pages 291 - 292, XP008029323 *

Also Published As

Publication number Publication date
WO2004018998A2 (en) 2004-03-04
AU2003272230A8 (en) 2004-03-11
AU2003272230A1 (en) 2004-03-11

Similar Documents

Publication Publication Date Title
WO2003062462A3 (en) Method for isolating nucleic acids and protein from a single sample
WO2003100012A3 (en) Microarrays and method for running hybridization reaction for multiple samples on a single microarray
WO2005071110A3 (en) Improving polynucleotide ligation reactions
WO2005068628A3 (en) Methods for nucleic acid isolation and kits using solid phase material
EP2305809A3 (en) Methods for nucleic acid isolation and kits using a microfluidic device and concentration step
WO2007100506A3 (en) Mass tags for quantitative analyses
WO2006108674A3 (en) Elite event a2704-12 and methods and kits for identifying such event in biological samples
AU2003259722A1 (en) Systems and methods for high-throughput microfluidic sample analysis
WO2004051218A8 (en) Multiplex amplification of polynucleotides
WO2008005321A3 (en) Systems and methods for real-time pcr
AU2002341621A1 (en) Methods, reagents, kits and apparatus for protein function analysis
WO2005017646A3 (en) System, software and methods for biomarker identification
WO2003060653A3 (en) Method and/or system for analyzing biological samples using a computer system
WO2005111212A3 (en) Biological bar code
WO2003040395A3 (en) Universal nucleotides for nucleic acid analysis
WO2007098427A3 (en) Massively multiplexed sequencing
WO2004048514A3 (en) Methods for analyzing a nucleic acid
WO2004092708A3 (en) Methods for the electrochemical detection of target compounds
WO2002088396A3 (en) Reagents and methods for automated in situ or microarray hybridization
CA2545989A1 (en) Method and device for purifying nucleic acids
WO2003031965A3 (en) Microfluidic extraction method
AU2003265768A1 (en) Automated framework for the analysis of biological data
AU2003229544A1 (en) A method for testing the interaction between at least one liquid sample and a respective solid sample.
AU2003297638A1 (en) Method for replacing used reaction cuvettes in an automatic analyzer depending upon next scheduled assay
WO2005031004A3 (en) Method for the specific rapid detection of beverage-spoiling micro-organisms

Legal Events

Date Code Title Description
AK Designated states

Kind code of ref document: A2

Designated state(s): AE AG AL AM AT AU AZ BA BB BG BR BY BZ CA CH CN CO CR CU CZ DE DK DM DZ EC EE ES FI GB GD GE GH GM HR HU ID IL IN IS JP KE KG KP KR KZ LC LK LR LS LT LU LV MA MD MG MK MN MW MX MZ NI NO NZ OM PG PH PL PT RO RU SC SD SE SG SK SL SY TJ TM TN TR TT TZ UA UG US UZ VC VN YU ZA ZM ZW

AL Designated countries for regional patents

Kind code of ref document: A2

Designated state(s): GH GM KE LS MW MZ SD SL SZ TZ UG ZM ZW AM AZ BY KG KZ MD RU TJ TM AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HU IE IT LU MC NL PT RO SE SI SK TR BF BJ CF CG CI CM GA GN GQ GW ML MR NE SN TD TG

121 Ep: the epo has been informed by wipo that ep was designated in this application
DFPE Request for preliminary examination filed prior to expiration of 19th month from priority date (pct application filed before 20040101)
122 Ep: pct application non-entry in european phase
NENP Non-entry into the national phase

Ref country code: JP

WWW Wipo information: withdrawn in national office

Country of ref document: JP