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WO2004072294A3 - Procedes et moyen de sequençage de sequences nucleotidiques - Google Patents

Procedes et moyen de sequençage de sequences nucleotidiques Download PDF

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Publication number
WO2004072294A3
WO2004072294A3 PCT/IB2004/000803 IB2004000803W WO2004072294A3 WO 2004072294 A3 WO2004072294 A3 WO 2004072294A3 IB 2004000803 W IB2004000803 W IB 2004000803W WO 2004072294 A3 WO2004072294 A3 WO 2004072294A3
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WO
WIPO (PCT)
Prior art keywords
strand
nucleotide complementarity
classes
nucleic acid
acid sequencing
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/IB2004/000803
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English (en)
Other versions
WO2004072294A2 (fr
Inventor
Sten Linnarsson
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GLOBAL GENOMICS AB
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GLOBAL GENOMICS AB
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Filing date
Publication date
Priority claimed from GB0303191A external-priority patent/GB2398383B/en
Application filed by GLOBAL GENOMICS AB filed Critical GLOBAL GENOMICS AB
Priority to EP04709304A priority Critical patent/EP1592810A2/fr
Priority to JP2006502489A priority patent/JP2006517798A/ja
Priority to CA002515938A priority patent/CA2515938A1/fr
Priority to US10/544,987 priority patent/US20060147935A1/en
Publication of WO2004072294A2 publication Critical patent/WO2004072294A2/fr
Publication of WO2004072294A3 publication Critical patent/WO2004072294A3/fr
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing

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  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

L'invention concerne la détermination de séquences nucléotidiques par séquençage. La synthèse amorcée d'un deuxième brin complémentaire d'un brin complémentaire dans des ensembles répétés d'étapes, chaque étape consistant à fournir une ou plusieurs des classes de complémentarité nucléotidiques à incorporer dans le brin synthétisé, et chaque ensemble d'étapes consistant é fournir les quatre classes complémentaires nucléotidiques possibles. Trois des quatre classes complémentaires nucléotidiques peuvent d'abord être fournies pour être incorporées dans le brin synthétisé, puis séparément la quatrième classe de complémentarité nucléotidique séparément. L'invention porte également sur une molécule d'ADN formée d'une partie tige et de première et seconde parties boucles, la partie tige comprenant un premier et un second brin, les premier et second brins étant de longueur équivalente, complémentaires et recuits ensemble, la première boucle reliant l'extrémité 3' du premier brin à l'extrémité 5' du second brin et la seconde boucle reliant l'extrémité 3' du second brin à l'extrémité 5' du premier brin, de sorte que la molécule d'ADN ne possède plus d'extrémité 5' ou 3' libre, et sur son utilisation.
PCT/IB2004/000803 2003-02-12 2004-02-09 Procedes et moyen de sequençage de sequences nucleotidiques Ceased WO2004072294A2 (fr)

Priority Applications (4)

Application Number Priority Date Filing Date Title
EP04709304A EP1592810A2 (fr) 2003-02-12 2004-02-09 Procedes et moyen de sequencage de sequences nucleotidiques
JP2006502489A JP2006517798A (ja) 2003-02-12 2004-02-09 核酸配列用の方法及び手段
CA002515938A CA2515938A1 (fr) 2003-02-12 2004-02-09 Procedes et moyen de sequencage de sequences nucleotidiques
US10/544,987 US20060147935A1 (en) 2003-02-12 2004-02-09 Methods and means for nucleic acid sequencing

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US44655303P 2003-02-12 2003-02-12
GB0303191.1 2003-02-12
US60/446,553 2003-02-12
GB0303191A GB2398383B (en) 2003-02-12 2003-02-12 Method and means for nucleic acid sequencing

Publications (2)

Publication Number Publication Date
WO2004072294A2 WO2004072294A2 (fr) 2004-08-26
WO2004072294A3 true WO2004072294A3 (fr) 2005-03-10

Family

ID=32870948

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/IB2004/000803 Ceased WO2004072294A2 (fr) 2003-02-12 2004-02-09 Procedes et moyen de sequençage de sequences nucleotidiques

Country Status (5)

Country Link
US (1) US20060147935A1 (fr)
EP (1) EP1592810A2 (fr)
JP (1) JP2006517798A (fr)
CA (1) CA2515938A1 (fr)
WO (1) WO2004072294A2 (fr)

Cited By (6)

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US8951731B2 (en) 2007-10-15 2015-02-10 Complete Genomics, Inc. Sequence analysis using decorated nucleic acids
US9228228B2 (en) 2006-10-27 2016-01-05 Complete Genomics, Inc. Efficient arrays of amplified polynucleotides
US9238834B2 (en) 2007-11-29 2016-01-19 Complete Genomics, Inc. Efficient shotgun sequencing methods
US9334490B2 (en) 2006-11-09 2016-05-10 Complete Genomics, Inc. Methods and compositions for large-scale analysis of nucleic acids using DNA deletions
US9476054B2 (en) 2005-06-15 2016-10-25 Complete Genomics, Inc. Two-adaptor library for high-throughput sequencing on DNA arrays
US9499863B2 (en) 2007-12-05 2016-11-22 Complete Genomics, Inc. Reducing GC bias in DNA sequencing using nucleotide analogs

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US7482120B2 (en) * 2005-01-28 2009-01-27 Helicos Biosciences Corporation Methods and compositions for improving fidelity in a nucleic acid synthesis reaction
EP2463386B1 (fr) 2005-06-15 2017-04-12 Complete Genomics Inc. Analyse d'acide nucléique par des mélanges aléatoires de fragments non chevauchants
GB0514910D0 (en) 2005-07-20 2005-08-24 Solexa Ltd Method for sequencing a polynucleotide template
GB0514935D0 (en) 2005-07-20 2005-08-24 Solexa Ltd Methods for sequencing a polynucleotide template
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JP2009529876A (ja) * 2006-03-14 2009-08-27 ゲニゾン バイオサイエンシス インコーポレイテッド 核酸を配列決定するための方法および手段
CN101460953B (zh) * 2006-03-31 2012-05-30 索雷克萨公司 用于合成分析的序列的系统和装置
US7754429B2 (en) 2006-10-06 2010-07-13 Illumina Cambridge Limited Method for pair-wise sequencing a plurity of target polynucleotides
WO2009003487A2 (fr) * 2007-06-29 2009-01-08 Unisense Fertilitech A/S Dispositif, système et procédé de surveillance et/ou de culture d'objets microscopiques
US8415099B2 (en) 2007-11-05 2013-04-09 Complete Genomics, Inc. Efficient base determination in sequencing reactions
WO2009097368A2 (fr) 2008-01-28 2009-08-06 Complete Genomics, Inc. Procédés et compositions pour un appel de base efficace dans des réactions de séquençage
US12060554B2 (en) 2008-03-10 2024-08-13 Illumina, Inc. Method for selecting and amplifying polynucleotides
US8628940B2 (en) 2008-09-24 2014-01-14 Pacific Biosciences Of California, Inc. Intermittent detection during analytical reactions
EP3170904B1 (fr) 2008-03-28 2017-08-16 Pacific Biosciences Of California, Inc. Compositions et procédés pour le séquençage d'acide nucléique
JP5843614B2 (ja) 2009-01-30 2016-01-13 オックスフォード ナノポア テクノロジーズ リミテッド 膜貫通配列決定における核酸構築物のためのアダプター
US9524369B2 (en) 2009-06-15 2016-12-20 Complete Genomics, Inc. Processing and analysis of complex nucleic acid sequence data
PT2669387T (pt) 2009-08-25 2016-09-20 Illumina Inc Métodos para selecção e amplificação de polinucleótidos
EP2580353B1 (fr) * 2010-06-11 2015-07-29 Life Technologies Corporation Flux alternatifs de nucléotides dans des procédés de séquençage par synthèse
US8666678B2 (en) 2010-10-27 2014-03-04 Life Technologies Corporation Predictive model for use in sequencing-by-synthesis
US10273540B2 (en) 2010-10-27 2019-04-30 Life Technologies Corporation Methods and apparatuses for estimating parameters in a predictive model for use in sequencing-by-synthesis
US9594870B2 (en) 2010-12-29 2017-03-14 Life Technologies Corporation Time-warped background signal for sequencing-by-synthesis operations
EP2658999B1 (fr) 2010-12-30 2019-03-13 Life Technologies Corporation Modèles pour analyser des données issues d'opérations de séquençage par synthèse
US20130060482A1 (en) 2010-12-30 2013-03-07 Life Technologies Corporation Methods, systems, and computer readable media for making base calls in nucleic acid sequencing
US10241075B2 (en) 2010-12-30 2019-03-26 Life Technologies Corporation Methods, systems, and computer readable media for nucleic acid sequencing
WO2012138921A1 (fr) 2011-04-08 2012-10-11 Life Technologies Corporation Ordonnancements d'écoulement de réactif de protection de phase pour l'utilisation dans un séquençage-par-synthèse
IN2014DN00221A (fr) 2011-07-25 2015-06-05 Oxford Nanopore Tech Ltd
US20150087537A1 (en) 2011-08-31 2015-03-26 Life Technologies Corporation Methods, Systems, Computer Readable Media, and Kits for Sample Identification
US10704164B2 (en) 2011-08-31 2020-07-07 Life Technologies Corporation Methods, systems, computer readable media, and kits for sample identification
JP6093498B2 (ja) * 2011-12-13 2017-03-08 株式会社日立ハイテクノロジーズ 核酸増幅方法
US9646132B2 (en) 2012-05-11 2017-05-09 Life Technologies Corporation Models for analyzing data from sequencing-by-synthesis operations
WO2014013259A1 (fr) 2012-07-19 2014-01-23 Oxford Nanopore Technologies Limited Procédé ssb
JP5663541B2 (ja) * 2012-09-19 2015-02-04 株式会社日立ハイテクノロジーズ 反応容器，並列処理装置、及びシーケンサ
US10329608B2 (en) 2012-10-10 2019-06-25 Life Technologies Corporation Methods, systems, and computer readable media for repeat sequencing
SG11201507138RA (en) 2013-03-08 2015-10-29 Oxford Nanopore Tech Ltd Enzyme stalling method
GB201314695D0 (en) 2013-08-16 2013-10-02 Oxford Nanopore Tech Ltd Method
US9146248B2 (en) 2013-03-14 2015-09-29 Intelligent Bio-Systems, Inc. Apparatus and methods for purging flow cells in nucleic acid sequencing instruments
US20140296080A1 (en) 2013-03-14 2014-10-02 Life Technologies Corporation Methods, Systems, and Computer Readable Media for Evaluating Variant Likelihood
US9591268B2 (en) 2013-03-15 2017-03-07 Qiagen Waltham, Inc. Flow cell alignment methods and systems
US9926597B2 (en) 2013-07-26 2018-03-27 Life Technologies Corporation Control nucleic acid sequences for use in sequencing-by-synthesis and methods for designing the same
EP3053072B1 (fr) 2013-10-04 2024-02-21 Life Technologies Corporation Procédés et systèmes de modélisation des effets de suppression dans le séquençage utilisant la chimie de terminaison
GB201403096D0 (en) 2014-02-21 2014-04-09 Oxford Nanopore Tech Ltd Sample preparation method
WO2016060974A1 (fr) 2014-10-13 2016-04-21 Life Technologies Corporation Procédés, systèmes, et supports lisibles par ordinateur pour appel de bases accéléré
GB201418159D0 (en) 2014-10-14 2014-11-26 Oxford Nanopore Tech Ltd Method
EP3295345B1 (fr) 2015-05-14 2023-01-25 Life Technologies Corporation Séquences de code-barre, et systèmes et procédés associés
CN106434873B (zh) * 2015-08-13 2021-08-27 生捷科技控股公司 使核酸分子同步化的方法
CN108699599B (zh) 2015-11-19 2026-01-06 赛纳生物科技(北京)有限公司 获得和校正生物序列信息的方法
US10619205B2 (en) 2016-05-06 2020-04-14 Life Technologies Corporation Combinatorial barcode sequences, and related systems and methods
GB201609220D0 (en) 2016-05-25 2016-07-06 Oxford Nanopore Tech Ltd Method
WO2018121587A1 (fr) 2016-12-27 2018-07-05 深圳华大生命科学研究院 Procédé de séquençage à base de colorant fluorescent unique
EP3775259A4 (fr) * 2018-03-26 2022-01-05 Ultima Genomics, Inc. Procédés de séquençage de molécules d'acide nucléique
GB201911421D0 (en) * 2019-08-09 2019-09-25 Dnae Diagnostics Ltd Methods of multiplexed isothermal amplification of nucleic acid sequences

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Cited By (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9476054B2 (en) 2005-06-15 2016-10-25 Complete Genomics, Inc. Two-adaptor library for high-throughput sequencing on DNA arrays
US9228228B2 (en) 2006-10-27 2016-01-05 Complete Genomics, Inc. Efficient arrays of amplified polynucleotides
US9334490B2 (en) 2006-11-09 2016-05-10 Complete Genomics, Inc. Methods and compositions for large-scale analysis of nucleic acids using DNA deletions
US8951731B2 (en) 2007-10-15 2015-02-10 Complete Genomics, Inc. Sequence analysis using decorated nucleic acids
US9238834B2 (en) 2007-11-29 2016-01-19 Complete Genomics, Inc. Efficient shotgun sequencing methods
US9499863B2 (en) 2007-12-05 2016-11-22 Complete Genomics, Inc. Reducing GC bias in DNA sequencing using nucleotide analogs

Also Published As

Publication number Publication date
CA2515938A1 (fr) 2004-08-26
WO2004072294A2 (fr) 2004-08-26
US20060147935A1 (en) 2006-07-06
EP1592810A2 (fr) 2005-11-09
JP2006517798A (ja) 2006-08-03

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