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WO2004064629A1 - Systeme detecteur servant a detecter des substances a analyser dans un liquide lacrymal - Google Patents

Systeme detecteur servant a detecter des substances a analyser dans un liquide lacrymal Download PDF

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Publication number
WO2004064629A1
WO2004064629A1 PCT/EP2004/000426 EP2004000426W WO2004064629A1 WO 2004064629 A1 WO2004064629 A1 WO 2004064629A1 EP 2004000426 W EP2004000426 W EP 2004000426W WO 2004064629 A1 WO2004064629 A1 WO 2004064629A1
Authority
WO
WIPO (PCT)
Prior art keywords
microchambers
hydrogel
sensor system
sensor
permeable
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/EP2004/000426
Other languages
English (en)
Inventor
Achim Müller
Wolfgang Ehrfeld
Karoly Nagy
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Novartis Pharma GmbH Austria
Novartis AG
Ehrfeld Miktotechnik AG
Original Assignee
Novartis Pharma GmbH Austria
Novartis AG
Ehrfeld Miktotechnik AG
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Novartis Pharma GmbH Austria, Novartis AG, Ehrfeld Miktotechnik AG filed Critical Novartis Pharma GmbH Austria
Publication of WO2004064629A1 publication Critical patent/WO2004064629A1/fr
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

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Classifications

    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
    • A61B5/00—Measuring for diagnostic purposes; Identification of persons
    • A61B5/145—Measuring characteristics of blood in vivo, e.g. gas concentration or pH-value ; Measuring characteristics of body fluids or tissues, e.g. interstitial fluid or cerebral tissue
    • A61B5/1455—Measuring characteristics of blood in vivo, e.g. gas concentration or pH-value ; Measuring characteristics of body fluids or tissues, e.g. interstitial fluid or cerebral tissue using optical sensors, e.g. spectral photometrical oximeters
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
    • A61B5/00—Measuring for diagnostic purposes; Identification of persons
    • A61B5/145—Measuring characteristics of blood in vivo, e.g. gas concentration or pH-value ; Measuring characteristics of body fluids or tissues, e.g. interstitial fluid or cerebral tissue
    • A61B5/14532—Measuring characteristics of blood in vivo, e.g. gas concentration or pH-value ; Measuring characteristics of body fluids or tissues, e.g. interstitial fluid or cerebral tissue for measuring glucose, e.g. by tissue impedance measurement
    • G—PHYSICS
    • G02—OPTICS
    • G02C—SPECTACLES; SUNGLASSES OR GOGGLES INSOFAR AS THEY HAVE THE SAME FEATURES AS SPECTACLES; CONTACT LENSES
    • G02C7/00—Optical parts
    • G02C7/02—Lenses; Lens systems ; Methods of designing lenses
    • G02C7/04—Contact lenses for the eyes

Definitions

  • the subject matter of the invention is a sensor system for detecting endogenous or exogenous analytes in tear fluid.
  • Soft contact lenses usually consist of hydrogels. These hydrogels are permeable to a number of substances contained in lachrymal fluid, such as salts, sugars, medicament constituents, proteins and other biomolecules, and may therefore serve as carriers for biosensors based on specific detection systems. With the assistance of a detection system immobilised in the lens material, individual substances (analytes) may thus be specifically optically detected. In this system, the spectroscopic characteristics of the detection system usually undergo a change that can be detected by appropriate measuring instruments.
  • the process of forming a bond or the release of a bond between a receptor and a ligand may be detected by means of the so-called Fluorescence Resonance Energy Transfer (FRET) by the change of fluorescence spectra of suitable dyes.
  • FRET Fluorescence Resonance Energy Transfer
  • a sensor molecule which specifically binds the analyte is labelled by a fluorescence marker (donor): the sensor molecule is bound to an analyte-like molecule, which likewise has fluorescence labelling (acceptor).
  • donor the fluorescence marker
  • acceptor the fluorescence labelling
  • FRET non-radiative energy transfer to the acceptor
  • the problem is therefore to overcome the above-mentioned disadvantages and to develop a detection system, which may be integrated into a soft contact lens and allows endogenous or exogenous substances in lachrymal fluid to be simply and reliably detected.
  • a sensor system which allows the detection of endogenous or exogenous substances in lachrymal fluid by means of a substance-specific detection system arranged preferably annularly or in another shape in a contact lens.
  • the detection system may be contained in annularly designed cavities or microchambers embedded into the hydrogel layer of a contact lens, the cavities or microchambers being sealed by a semi-permeable membrane.
  • the detection system may be contained in annularly designed microchambers, which are formed from semi-permeable films and are embedded into the hydrogel layer of a contact lens.
  • the detection system may also be contained in a spirally designed microtube, which has a semi-permeable wall and is embedded into the hydrogel layer of a contact lens.
  • the fluorescent sensor molecules which are enclosed in the microchambers or in the microtube, but are freely mobile in the solution. They are prevented from escaping from the chambers by the semi-permeable walls, whereby the diffusion selectivity (cut-off) of these walls is set such that the analyte molecules can simultaneously diffuse freely into the chambers. Owing to the free mobility of the sensor molecules in the microchambers and microtubes, they retain their full sensitivity and reaction kinetics.
  • the wall of the microchambers or of the microtube is chosen so that it is permeable to molecules up to a diameter of 5000 Daltons.
  • a further advantage of the above-mentioned microchamber concept is the possibility of including various sensor molecules in the detection system at a distance from each other.
  • the different designs of the object according to the invention are illustrated in detail as follows:
  • the lens-specific hydrogel has a certain permeability for the sensor and marker molecules
  • another hydrogel or a more strongly crosslinked form of the lens material is used for encapsulation of the sensor solution, so as to ensure safer enclosure of the sensor molecules with simultaneous unhindered diffusion of the analyte.
  • the geometry of the finished object is selected such that it can be integrated into the lens without problems (fig. 1 ). So as not to impair vision through the centre of the lens, a ring-shaped geometry of the microstructure is offered in particular (“sensor ring”), though other shapes may similarly be conceived.
  • recesses are inserted into the hydrogel surface (fig. 2). This may take place, for example, by stamping when the hydrogel is crosslinked or by subsequently cutting out with a laser.
  • the dimensions and spacing of the microchambers are chosen such that their density and mechanical strength are assured whilst simultaneously providing flexibility of the microstructure.
  • the chambers may have a round or square shape.
  • the microchambers filled with the sensor solution are covered with a thin semi-permeable film (for example dialysis film) or with a thin hydrogel layer. The strength of the whole system is less than the strength of the lens. This dimension applies to all further sensor rings.
  • the hydrogel and the crosslinking thereof are adapted such that the sensor molecules remain firmly enclosed in the microchambers and the analyte can diffuse in.
  • the microchambers may be formed directly in the lens material. However, the selective permeability of the chamber walls must be guaranteed. This can be assured by a coating of the chamber walls (fig. 3). The selective permeability of the coating enables free diffusion of the analytes to take place and blocks diffusion of the detection system.
  • the filled microchambers are covered with a semi-permeable film (dialysis film) or with a similarly coated hydrogel layer (fig. 3).
  • microscopic drops of the detection system may be injected into the still liquid hydrogel (fig. 4). After rapid polymerisation, the drops remain in the hydrogel.
  • the hydrogel and its crosslinking are chosen so as to be suitable for the said prerequisites.
  • the microchambers may be formed directly in semi-permeable membranes (dialysis films).
  • An example is shown in fig. 5.
  • This structure consists of three layers/films: one thicker layer, in which the microchambers are shaped, and two thin coverings. If all films have the same properties (selective permeability, cutoff), optimum functioning may be expected; however, selective permeability is only necessary for a covering film.
  • the dimensions and pattern of the microchambers may be chosen as in section 1. 2.2 Structured film with microchambers
  • Thin films can be easily structured, whereupon it is possible to create the microchambers.
  • Fig. 6 shows an example.
  • the microchambers formed by stamping and filled with sensor solution are sealed by a covering film. Permeability and cut-off of the films are chosen in accordance with the relevant detection method.
  • microcapillaries may have an advantage, since filling of the microstructures with sensor solution is simplified in this case by capillary forces.
  • the capillary chambers may be arranged radially or in concentric circles (fig. 8). First of all, a wavy structure is formed from a film, which is sealed on both sides by covering films. The capillary chambers are then filled with the sensor solution by capillary forces.
  • This embodiment is similar to that above (2.4), but here the concentric rings are not capillaries, but are flat ring chambers (fig. 9). After filling the ring chambers, a covering film is used to seal in the sensor solution.
  • a simple solution is offered by the usage of a dialysis tube of an appropriate dimension (for example a hollow fibre) (fig. 10). After filling the microtube with the sensor solution, it is coiled up. A snake-like structure is also conceivable. Subsequently, hydrogel may be used to fix the stricture (see production methods below).
  • the illustrated designs of the microstructured contact lens can be produced efficiently by mass production.
  • the parallel production process comes to the fore here.
  • Parallel shaping and filling of hundreds of sensor rings may be carried out for the illustrated concepts (with the exception of the dialysis tube) (figs. 11 and
  • Microstructuring of the sensor rings is carried out on gel layers or films having a large area (fig. 11 ).
  • various operational methods may be used:
  • the system should be made up of a film which is non- transparent (absorbing) to the laser and from a transparent film.
  • the laser beam is focussed onto the level of the contact area of the two films.
  • the surface of the absorbing film is warmed up locally and welded to the transparent film.
  • the microchambers in the hydrogel may be shaped in different ways, whereby the hydrogel used for the sensor ring is not identical to that of the lens material.
  • Figs. 14 and 15 illustrate two examples.
  • the microstructure in the hydrogel is formed by a stamp. After polymerisation, the desired structure is obtained.
  • the filled microchambers are sealed by a thin covering film (dialysis film) or by a thin hydrogel layer.
  • the hydrogel layer should be only slightly polymerised, so that handling is possible. Complete polymerisation should be carried out after covering the microchambers. In this operating step, the edges of the microchambers and the covering layer are joined together.
  • microchambers in the polymerised hydrogel may likewise be shaped by laser ablation (fig. 15). The remaining steps of this production process are identical to the above method.
  • the contact lens consists of two layers. In one layer, the microchambers are shaped in the course of the molding process (fig. 16). After coating the chamber walls, the chambers are filled up. The chambers are sealed by the second lens part which is similarly coated. The second lens part is only slightly polymerised prior to covering, as described above, and, after covering, polymerisation is concluded in order to seal the chambers safely.
  • the second lens part can be replaced by a dialysis film.
  • the microchambers should similarly be arranged in annular manner around the edge of the lens, in order to maintain compete quality of vision.
  • the production process of this embodiment is shown in fig. 17.
  • the sensor solution Prior to polymerisation, the sensor solution is injected into the hydrogel by an injection system. After rapid crosslinking, the microdrops remain in the hydrogel at the desired depth.
  • microdrops it is similarly conceivable for the microdrops to be injected directly into the lens material.
  • a further crosslinker is mixed into the sensor solution, and leads to further polymerisation at the walls of the cavities, thereby ensuring the desired selective permeability.
  • the production process for the sensor ring consisting of a multi-layered film is illustrated in fig. 18. First of all, the thick middle layer is worked. The chamber structure is shaped by stamping or by laser. The middle layer is then welded together with the lower film, as demonstrated in fig. 13. After filling the microchambers, an appropriate welding ⁇ procedure takes place, in order to seal the chambers. 5. Structured film with microchambers
  • Structuring of a thin film may be carried out in two different ways (figs. 19 and 20).
  • a stamp and a mold are used (fig. 19).
  • the recesses are filled with the sensor solution and sealed.
  • fig. 20 only a stamp is used, which presses the film into the viscous, lens-specific hydrogel.
  • the subsequent polymerisation ensures the structure. After filling, first of all a covering film is applied and finally the upper lens part.
  • the second lens part is unnecessary, if the covering film does not have sharp edges and/or the full lens surface is covered and the otherwise customary characteristics of the contact lens are not changed (for example oxygen permeability, comfort, etc.).
  • Microstructuring of the dialysis film, as well as filling and sealing, are carried out in a similar manner to that above (5) (fig. 21 ). Subsequently, the ring is turned over and the intermediate spaces are similarly filled. Covering is carried out as usual with a further film.
  • the microstructure is stamped into the film with stamps and molds (fig. 22). Subsequently, covering films are welded to the top and bottom of the structured middle film, whereby both ends of the capillary chamber should remain open.
  • the capillaries are filled by capillary forces.
  • the capillary chambers are sealed by the pressing and welding operations.
  • processing steps for the film may be carried out in a similar manner to the above (fig. 23).
  • Fig. 24 shows a schematic illustration of the production steps for the sensor ring.
  • the tube is filled with the sensor solution.
  • the tube is wound onto a holder, the base of which is coated with hydrogel that has not yet been polymerised.
  • the tube spool is coated from the top with a further layer of hydrogel. The hydrogel holds the spool in the desired shape after polymerisation.
  • Small optical signals are measured by the sensor ring.
  • a reflecting layer is applied to the rear surface of the ring.
  • the finished sensor rings are checked for the following qualities prior to integration into the contact lens:
  • the sensor rings may be integrated into the contact lens in various ways.
  • One possibility is a three-layered contact lens, as has already been used for coloured contact lenses (EP 0 369 942 A1).
  • the contact lens consists of a lower layer, middle film layer with the sensor ring and an upper layer (fig. 25 left).
  • the sensor rings can be stamped out of a film of large area, placed into the lens mold and hydrogel poured around them (fig. 25 right). In this case, knubs should be inserted on the film or in the mold, in order to keep the ring at a defined distance from the surface of the contact lens.

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  • Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Optics & Photonics (AREA)
  • General Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Heart & Thoracic Surgery (AREA)
  • Veterinary Medicine (AREA)
  • Biophysics (AREA)
  • Pathology (AREA)
  • Ophthalmology & Optometry (AREA)
  • Biomedical Technology (AREA)
  • Public Health (AREA)
  • Medical Informatics (AREA)
  • Molecular Biology (AREA)
  • Surgery (AREA)
  • Animal Behavior & Ethology (AREA)
  • Spectroscopy & Molecular Physics (AREA)
  • General Physics & Mathematics (AREA)
  • Emergency Medicine (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)

Abstract

L'invention concerne un système détecteur servant à détecter des substances endogènes ou exogènes dans un liquide lacrymal, ledit système comprenant un système de détection, spécifique à la substance, contenu dans une lentille de contact. Ce système de détection peut être contenu dans des cavités, des microchambres, ou des microtubes, ou même dans des microchambres formées à partir de films semi-perméables, qui sont incorporés dans une couche d'hydrogel de la lentille de contact.
PCT/EP2004/000426 2003-01-21 2004-01-20 Systeme detecteur servant a detecter des substances a analyser dans un liquide lacrymal Ceased WO2004064629A1 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP03001153 2003-01-21
EP03001153.0 2003-01-21

Publications (1)

Publication Number Publication Date
WO2004064629A1 true WO2004064629A1 (fr) 2004-08-05

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PCT/EP2004/000426 Ceased WO2004064629A1 (fr) 2003-01-21 2004-01-20 Systeme detecteur servant a detecter des substances a analyser dans un liquide lacrymal

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Cited By (58)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7137952B2 (en) 2001-06-29 2006-11-21 Ecole Polytechnique Federale De Lausanne-Service Des Relations Industrielles Intraocular pressure recording system
WO2008142158A3 (fr) * 2007-05-24 2009-05-28 Eyesense Ag Implant d'hydrogel pour la détection par capteurs de métabolites dans des tissus corporels
WO2014052125A1 (fr) * 2012-09-26 2014-04-03 Google Inc. Facilitation du recueil et de l'analyse d'échantillons de liquide lacrymal à l'aide d'une lentille de contact
US8798332B2 (en) 2012-05-15 2014-08-05 Google Inc. Contact lenses
US8821811B2 (en) 2012-09-26 2014-09-02 Google Inc. In-vitro contact lens testing
US8820934B1 (en) 2012-09-05 2014-09-02 Google Inc. Passive surface acoustic wave communication
US8857981B2 (en) 2012-07-26 2014-10-14 Google Inc. Facilitation of contact lenses with capacitive sensors
US8874182B2 (en) 2013-01-15 2014-10-28 Google Inc. Encapsulated electronics
US8870370B1 (en) 2012-09-24 2014-10-28 Google Inc. Contact lens that facilitates antenna communication via sensor impedance modulation
US8880139B1 (en) 2013-06-17 2014-11-04 Google Inc. Symmetrically arranged sensor electrodes in an ophthalmic electrochemical sensor
KR101456306B1 (ko) 2006-05-03 2014-11-04 더 차이니즈 유니버시티 오브 홍콩 산전 진단 및 모니터링을 위한 신규 태아 마커
WO2014189892A1 (fr) * 2013-05-21 2014-11-27 Johnson & Johnson Vision Care, Inc. Lentille ophtalmique dotée d'un système de coloration basé sur un événement passif
US20140346695A1 (en) * 2013-05-21 2014-11-27 Johnson & Johnson Vision Care, Inc. Method of manufacturing an ophthalmic lens with a passive event-based coloration system
US8909311B2 (en) 2012-08-21 2014-12-09 Google Inc. Contact lens with integrated pulse oximeter
US8919953B1 (en) 2012-08-02 2014-12-30 Google Inc. Actuatable contact lenses
WO2014210526A1 (fr) * 2013-06-28 2014-12-31 Google Inc. Procédé pour former un canal à travers une couche de polymère au moyen d'une ou plusieurs couches de résine photosensible
US8926809B2 (en) 2013-01-25 2015-01-06 Google Inc. Standby biasing of electrochemical sensor to reduce sensor stabilization time during measurement
US20150037262A1 (en) * 2012-01-27 2015-02-05 Ecole Polytechnique Federale De Lausanne (Epfl) Device for the transplantation of cells in suspension
US8950068B2 (en) 2013-03-26 2015-02-10 Google Inc. Systems and methods for encapsulating electronics in a mountable device
US8965478B2 (en) 2012-10-12 2015-02-24 Google Inc. Microelectrodes in an ophthalmic electrochemical sensor
US8960899B2 (en) 2012-09-26 2015-02-24 Google Inc. Assembling thin silicon chips on a contact lens
US8960898B1 (en) 2012-09-24 2015-02-24 Google Inc. Contact lens that restricts incoming light to the eye
US8979271B2 (en) 2012-09-25 2015-03-17 Google Inc. Facilitation of temperature compensation for contact lens sensors and temperature sensing
US8985763B1 (en) 2012-09-26 2015-03-24 Google Inc. Contact lens having an uneven embedded substrate and method of manufacture
US8989834B2 (en) 2012-09-25 2015-03-24 Google Inc. Wearable device
US9009958B2 (en) 2013-03-27 2015-04-21 Google Inc. Systems and methods for encapsulating electronics in a mountable device
EP2761364A4 (fr) * 2011-09-29 2015-05-13 Ehren Burton Lentilles de contact à changement de couleur
US9063351B1 (en) 2012-09-28 2015-06-23 Google Inc. Input detection system
US9111473B1 (en) 2012-08-24 2015-08-18 Google Inc. Input system
EP2906989A4 (fr) * 2013-01-17 2015-09-16 Google Inc Procédé de mise en place d'une structure en forme d'anneau dans un dispositif à pose oculaire
US9158133B1 (en) 2012-07-26 2015-10-13 Google Inc. Contact lens employing optical signals for power and/or communication
US9176332B1 (en) 2012-10-24 2015-11-03 Google Inc. Contact lens and method of manufacture to improve sensor sensitivity
US9184698B1 (en) 2014-03-11 2015-11-10 Google Inc. Reference frequency from ambient light signal
US9298020B1 (en) 2012-07-26 2016-03-29 Verily Life Sciences Llc Input system
US9307901B1 (en) 2013-06-28 2016-04-12 Verily Life Sciences Llc Methods for leaving a channel in a polymer layer using a cross-linked polymer plug
US9320460B2 (en) 2012-09-07 2016-04-26 Verily Life Sciences Llc In-situ tear sample collection and testing using a contact lens
US9326710B1 (en) 2012-09-20 2016-05-03 Verily Life Sciences Llc Contact lenses having sensors with adjustable sensitivity
US9332935B2 (en) 2013-06-14 2016-05-10 Verily Life Sciences Llc Device having embedded antenna
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US9523865B2 (en) 2012-07-26 2016-12-20 Verily Life Sciences Llc Contact lenses with hybrid power sources
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US9636050B1 (en) 2013-11-07 2017-05-02 Verily Life Sciences Llc Methods and apparatus for forming a channel through a polymer layer using a protrusion
US9654674B1 (en) 2013-12-20 2017-05-16 Verily Life Sciences Llc Image sensor with a plurality of light channels
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US9696564B1 (en) 2012-08-21 2017-07-04 Verily Life Sciences Llc Contact lens with metal portion and polymer layer having indentations
CN107072602A (zh) * 2014-09-04 2017-08-18 威里利生命科学有限责任公司 通道形成
US9757056B1 (en) 2012-10-26 2017-09-12 Verily Life Sciences Llc Over-molding of sensor apparatus in eye-mountable device
US9789655B1 (en) 2014-03-14 2017-10-17 Verily Life Sciences Llc Methods for mold release of body-mountable devices including microelectronics
US9814387B2 (en) 2013-06-28 2017-11-14 Verily Life Sciences, LLC Device identification
US9884180B1 (en) 2012-09-26 2018-02-06 Verily Life Sciences Llc Power transducer for a retinal implant using a contact lens
US9948895B1 (en) 2013-06-18 2018-04-17 Verily Life Sciences Llc Fully integrated pinhole camera for eye-mountable imaging system
US9965583B2 (en) 2012-09-25 2018-05-08 Verily Life Sciences, LLC Information processing method
US10001661B1 (en) 2014-03-06 2018-06-19 Verily Life Sciences Llc Body-mountable devices having an optical polarizer
US10010270B2 (en) 2012-09-17 2018-07-03 Verily Life Sciences Llc Sensing system
US10232531B1 (en) 2014-07-08 2019-03-19 Verily Life Sciences Llc Methods and apparatus for forming a polymer layer around a structure using a plurality of protrusions

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2001013783A1 (fr) * 1999-08-26 2001-03-01 Novartis Ag Detecteur d'analyte dans un liquide oculaire
WO2002003855A1 (fr) * 2000-07-07 2002-01-17 Christopher John Stanley Dispositif optique de mesure d'analysats dans des larmes
US20020049389A1 (en) * 1996-09-04 2002-04-25 Abreu Marcio Marc Noninvasive measurement of chemical substances

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20020049389A1 (en) * 1996-09-04 2002-04-25 Abreu Marcio Marc Noninvasive measurement of chemical substances
WO2001013783A1 (fr) * 1999-08-26 2001-03-01 Novartis Ag Detecteur d'analyte dans un liquide oculaire
WO2002003855A1 (fr) * 2000-07-07 2002-01-17 Christopher John Stanley Dispositif optique de mesure d'analysats dans des larmes

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
MARCH W F ET AL: "INTRAOCULAR LENS GLUCOSE SENSOR", DIABETES TECHNOLOGY AND THERAPEUTICS, MARY ANN LIEBERT, LARCHMONT, NY, US, vol. 2, no. 1, 2000, pages 27 - 30, XP001028056, ISSN: 1520-9156 *
RUSSELL R J ET AL: "A FLUORESCENCE-BASED GLUCOSE BIOSENSOR USING CONCANAVALIN A AND DEXTRAN ENCAPSULATED IN A POLY(ETHYLENE GLYCOL) HYDROGEL", ANALYTICAL CHEMISTRY, AMERICAN CHEMICAL SOCIETY. COLUMBUS, US, vol. 71, no. 15, 1 August 1999 (1999-08-01), pages 3126 - 3132, XP000851425, ISSN: 0003-2700 *

Cited By (97)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7137952B2 (en) 2001-06-29 2006-11-21 Ecole Polytechnique Federale De Lausanne-Service Des Relations Industrielles Intraocular pressure recording system
KR101456306B1 (ko) 2006-05-03 2014-11-04 더 차이니즈 유니버시티 오브 홍콩 산전 진단 및 모니터링을 위한 신규 태아 마커
WO2008142158A3 (fr) * 2007-05-24 2009-05-28 Eyesense Ag Implant d'hydrogel pour la détection par capteurs de métabolites dans des tissus corporels
AU2008252936B2 (en) * 2007-05-24 2013-11-07 Eyesense Ag Hydrogel implant for sensing metabolites in body tissue
US8647271B2 (en) 2007-05-24 2014-02-11 Eyesense Ag Hydrogel implant for sensing metabolites in body tissue
EP2761364A4 (fr) * 2011-09-29 2015-05-13 Ehren Burton Lentilles de contact à changement de couleur
US20150037262A1 (en) * 2012-01-27 2015-02-05 Ecole Polytechnique Federale De Lausanne (Epfl) Device for the transplantation of cells in suspension
US9047512B2 (en) 2012-05-15 2015-06-02 Google Inc. Contact lenses
US8798332B2 (en) 2012-05-15 2014-08-05 Google Inc. Contact lenses
US9158133B1 (en) 2012-07-26 2015-10-13 Google Inc. Contact lens employing optical signals for power and/or communication
US10256919B1 (en) 2012-07-26 2019-04-09 Verily Life Sciences Llc Employing optical signals for power and/or communication
US9523865B2 (en) 2012-07-26 2016-12-20 Verily Life Sciences Llc Contact lenses with hybrid power sources
US10873401B1 (en) 2012-07-26 2020-12-22 Verily Life Sciences Llc Employing optical signals for power and/or communication
US8864305B2 (en) 2012-07-26 2014-10-21 Google Inc. Facilitation of contact lenses with capacitive sensors
US9735892B1 (en) 2012-07-26 2017-08-15 Verily Life Sciences Llc Employing optical signals for power and/or communication
US9298020B1 (en) 2012-07-26 2016-03-29 Verily Life Sciences Llc Input system
US8857981B2 (en) 2012-07-26 2014-10-14 Google Inc. Facilitation of contact lenses with capacitive sensors
US10120203B2 (en) 2012-07-26 2018-11-06 Verliy Life Sciences LLC Contact lenses with hybrid power sources
US8919953B1 (en) 2012-08-02 2014-12-30 Google Inc. Actuatable contact lenses
US8909311B2 (en) 2012-08-21 2014-12-09 Google Inc. Contact lens with integrated pulse oximeter
US8971978B2 (en) 2012-08-21 2015-03-03 Google Inc. Contact lens with integrated pulse oximeter
US9696564B1 (en) 2012-08-21 2017-07-04 Verily Life Sciences Llc Contact lens with metal portion and polymer layer having indentations
US9111473B1 (en) 2012-08-24 2015-08-18 Google Inc. Input system
US8820934B1 (en) 2012-09-05 2014-09-02 Google Inc. Passive surface acoustic wave communication
US9320460B2 (en) 2012-09-07 2016-04-26 Verily Life Sciences Llc In-situ tear sample collection and testing using a contact lens
US10729363B1 (en) 2012-09-11 2020-08-04 Verily Life Sciences Llc Cancellation of a baseline current signal via current subtraction within a linear relaxation oscillator-based current-to-frequency converter circuit
US9737248B1 (en) 2012-09-11 2017-08-22 Verily Life Sciences Llc Cancellation of a baseline current signal via current subtraction within a linear relaxation oscillator-based current-to-frequency converter circuit
US9398868B1 (en) 2012-09-11 2016-07-26 Verily Life Sciences Llc Cancellation of a baseline current signal via current subtraction within a linear relaxation oscillator-based current-to-frequency converter circuit
US10010270B2 (en) 2012-09-17 2018-07-03 Verily Life Sciences Llc Sensing system
US10932695B2 (en) 2012-09-17 2021-03-02 Verily Life Sciences Llc Sensing system
US9326710B1 (en) 2012-09-20 2016-05-03 Verily Life Sciences Llc Contact lenses having sensors with adjustable sensitivity
US8960898B1 (en) 2012-09-24 2015-02-24 Google Inc. Contact lens that restricts incoming light to the eye
US8870370B1 (en) 2012-09-24 2014-10-28 Google Inc. Contact lens that facilitates antenna communication via sensor impedance modulation
US8979271B2 (en) 2012-09-25 2015-03-17 Google Inc. Facilitation of temperature compensation for contact lens sensors and temperature sensing
US9965583B2 (en) 2012-09-25 2018-05-08 Verily Life Sciences, LLC Information processing method
US8989834B2 (en) 2012-09-25 2015-03-24 Google Inc. Wearable device
US9488853B2 (en) 2012-09-26 2016-11-08 Verily Life Sciences Llc Assembly bonding
US8960899B2 (en) 2012-09-26 2015-02-24 Google Inc. Assembling thin silicon chips on a contact lens
US10099049B2 (en) 2012-09-26 2018-10-16 Verily Life Sciences Llc Power transducer for a retinal implant using using a contact lens
US8985763B1 (en) 2012-09-26 2015-03-24 Google Inc. Contact lens having an uneven embedded substrate and method of manufacture
US9054079B2 (en) 2012-09-26 2015-06-09 Google Inc. Assembling thin silicon chips on a contact lens
US8821811B2 (en) 2012-09-26 2014-09-02 Google Inc. In-vitro contact lens testing
US9884180B1 (en) 2012-09-26 2018-02-06 Verily Life Sciences Llc Power transducer for a retinal implant using a contact lens
WO2014052125A1 (fr) * 2012-09-26 2014-04-03 Google Inc. Facilitation du recueil et de l'analyse d'échantillons de liquide lacrymal à l'aide d'une lentille de contact
US9063351B1 (en) 2012-09-28 2015-06-23 Google Inc. Input detection system
US10342424B2 (en) 2012-09-28 2019-07-09 Verily Life Sciences Llc Input detection system
US9775513B1 (en) 2012-09-28 2017-10-03 Verily Life Sciences Llc Input detection system
US9724027B2 (en) 2012-10-12 2017-08-08 Verily Life Sciences Llc Microelectrodes in an ophthalmic electrochemical sensor
US9055902B2 (en) 2012-10-12 2015-06-16 Google Inc. Microelectrodes in an ophthalmic electrochemical sensor
US8965478B2 (en) 2012-10-12 2015-02-24 Google Inc. Microelectrodes in an ophthalmic electrochemical sensor
US9176332B1 (en) 2012-10-24 2015-11-03 Google Inc. Contact lens and method of manufacture to improve sensor sensitivity
US9757056B1 (en) 2012-10-26 2017-09-12 Verily Life Sciences Llc Over-molding of sensor apparatus in eye-mountable device
US10004457B2 (en) 2013-01-15 2018-06-26 Verily Life Sciences Llc Encapsulated electronics
US8874182B2 (en) 2013-01-15 2014-10-28 Google Inc. Encapsulated electronics
US8886275B2 (en) 2013-01-15 2014-11-11 Google Inc. Encapsulated electronics
US9289954B2 (en) 2013-01-17 2016-03-22 Verily Life Sciences Llc Method of ring-shaped structure placement in an eye-mountable device
EP2906989A4 (fr) * 2013-01-17 2015-09-16 Google Inc Procédé de mise en place d'une structure en forme d'anneau dans un dispositif à pose oculaire
CN104937478A (zh) * 2013-01-17 2015-09-23 谷歌公司 在眼部可安装设备中布置环形结构的方法
JP2016507074A (ja) * 2013-01-17 2016-03-07 グーグル インコーポレイテッド 眼部装着可能型デバイスにおける環状構造の位置決めの方法
CN104937478B (zh) * 2013-01-17 2017-03-29 威里利生命科学有限责任公司 在眼部可安装设备中布置环形结构的方法
US8926809B2 (en) 2013-01-25 2015-01-06 Google Inc. Standby biasing of electrochemical sensor to reduce sensor stabilization time during measurement
US9636016B1 (en) 2013-01-25 2017-05-02 Verily Life Sciences Llc Eye-mountable devices and methods for accurately placing a flexible ring containing electronics in eye-mountable devices
US9161712B2 (en) 2013-03-26 2015-10-20 Google Inc. Systems and methods for encapsulating electronics in a mountable device
US8950068B2 (en) 2013-03-26 2015-02-10 Google Inc. Systems and methods for encapsulating electronics in a mountable device
US9009958B2 (en) 2013-03-27 2015-04-21 Google Inc. Systems and methods for encapsulating electronics in a mountable device
US9113829B2 (en) 2013-03-27 2015-08-25 Google Inc. Systems and methods for encapsulating electronics in a mountable device
JP2016521862A (ja) * 2013-05-21 2016-07-25 ジョンソン・アンド・ジョンソン・ビジョン・ケア・インコーポレイテッドＪｏｈｎｓｏｎ ＆ Ｊｏｈｎｓｏｎ Ｖｉｓｉｏｎ Ｃａｒｅ， Ｉｎｃ． 受動的な事象に基づく着色システムを有する眼用レンズを製造する方法
US20140346695A1 (en) * 2013-05-21 2014-11-27 Johnson & Johnson Vision Care, Inc. Method of manufacturing an ophthalmic lens with a passive event-based coloration system
WO2014189892A1 (fr) * 2013-05-21 2014-11-27 Johnson & Johnson Vision Care, Inc. Lentille ophtalmique dotée d'un système de coloration basé sur un événement passif
JP2016526187A (ja) * 2013-05-21 2016-09-01 ジョンソン・アンド・ジョンソン・ビジョン・ケア・インコーポレイテッドＪｏｈｎｓｏｎ ＆ Ｊｏｈｎｓｏｎ Ｖｉｓｉｏｎ Ｃａｒｅ， Ｉｎｃ． 受動的な事象に基づく着色システムを有する眼用レンズ
AU2014268742B2 (en) * 2013-05-21 2018-01-18 Johnson & Johnson Vision Care, Inc. An Ophthalmic Lens with a passive event-based coloration system
US9915829B2 (en) * 2013-05-21 2018-03-13 Johnson & Johnson Vision Care, Inc. Method of manufacturing an ophthalmic lens with a passive event-based coloration system
CN105209962A (zh) * 2013-05-21 2015-12-30 庄臣及庄臣视力保护公司 具有基于事件的无源着色系统的眼科镜片
US9332935B2 (en) 2013-06-14 2016-05-10 Verily Life Sciences Llc Device having embedded antenna
JP2017060868A (ja) * 2013-06-17 2017-03-30 ヴェリリー ライフ サイエンシズ エルエルシー 眼科用電気化学センサ内で対称配置されるセンサ電極
US9662054B2 (en) 2013-06-17 2017-05-30 Verily Life Sciences Llc Symmetrically arranged sensor electrodes in an ophthalmic electrochemical sensor
JP2016526412A (ja) * 2013-06-17 2016-09-05 ヴェリリー ライフ サイエンシズ エルエルシー 眼科用電気化学センサ内で対称配置されるセンサ電極
US9084561B2 (en) 2013-06-17 2015-07-21 Google Inc. Symmetrically arranged sensor electrodes in an ophthalmic electrochemical sensor
US8880139B1 (en) 2013-06-17 2014-11-04 Google Inc. Symmetrically arranged sensor electrodes in an ophthalmic electrochemical sensor
US9948895B1 (en) 2013-06-18 2018-04-17 Verily Life Sciences Llc Fully integrated pinhole camera for eye-mountable imaging system
US9685689B1 (en) 2013-06-27 2017-06-20 Verily Life Sciences Llc Fabrication methods for bio-compatible devices
US9307901B1 (en) 2013-06-28 2016-04-12 Verily Life Sciences Llc Methods for leaving a channel in a polymer layer using a cross-linked polymer plug
US9492118B1 (en) 2013-06-28 2016-11-15 Life Sciences Llc Pre-treatment process for electrochemical amperometric sensor
WO2014210526A1 (fr) * 2013-06-28 2014-12-31 Google Inc. Procédé pour former un canal à travers une couche de polymère au moyen d'une ou plusieurs couches de résine photosensible
US9028772B2 (en) 2013-06-28 2015-05-12 Google Inc. Methods for forming a channel through a polymer layer using one or more photoresist layers
US9814387B2 (en) 2013-06-28 2017-11-14 Verily Life Sciences, LLC Device identification
US9636050B1 (en) 2013-11-07 2017-05-02 Verily Life Sciences Llc Methods and apparatus for forming a channel through a polymer layer using a protrusion
US9654674B1 (en) 2013-12-20 2017-05-16 Verily Life Sciences Llc Image sensor with a plurality of light channels
US9572522B2 (en) 2013-12-20 2017-02-21 Verily Life Sciences Llc Tear fluid conductivity sensor
US10001661B1 (en) 2014-03-06 2018-06-19 Verily Life Sciences Llc Body-mountable devices having an optical polarizer
US9366570B1 (en) 2014-03-10 2016-06-14 Verily Life Sciences Llc Photodiode operable in photoconductive mode and photovoltaic mode
US9184698B1 (en) 2014-03-11 2015-11-10 Google Inc. Reference frequency from ambient light signal
US9789655B1 (en) 2014-03-14 2017-10-17 Verily Life Sciences Llc Methods for mold release of body-mountable devices including microelectronics
US10232531B1 (en) 2014-07-08 2019-03-19 Verily Life Sciences Llc Methods and apparatus for forming a polymer layer around a structure using a plurality of protrusions
CN107072602A (zh) * 2014-09-04 2017-08-18 威里利生命科学有限责任公司 通道形成
US9907498B2 (en) 2014-09-04 2018-03-06 Verily Life Sciences Llc Channel formation
EP3169217A4 (fr) * 2014-09-04 2018-02-14 Verily Life Sciences LLC Formation de canal

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