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WO2004048528A3 - Sampling method and apparatus for amplification reaction analysis - Google Patents

Sampling method and apparatus for amplification reaction analysis Download PDF

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Publication number
WO2004048528A3
WO2004048528A3 PCT/US2003/037420 US0337420W WO2004048528A3 WO 2004048528 A3 WO2004048528 A3 WO 2004048528A3 US 0337420 W US0337420 W US 0337420W WO 2004048528 A3 WO2004048528 A3 WO 2004048528A3
Authority
WO
WIPO (PCT)
Prior art keywords
amplification
amplification reaction
quantitative
sampling method
reaction analysis
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/US2003/037420
Other languages
French (fr)
Other versions
WO2004048528A2 (en
Inventor
Vladimir I Slepnev
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Sention Inc
Original Assignee
Sention Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Sention Inc filed Critical Sention Inc
Priority to CA002509769A priority Critical patent/CA2509769A1/en
Priority to AU2003291144A priority patent/AU2003291144A1/en
Priority to JP2005510386A priority patent/JP2006507849A/en
Priority to EP03783738A priority patent/EP1563097A4/en
Publication of WO2004048528A2 publication Critical patent/WO2004048528A2/en
Publication of WO2004048528A3 publication Critical patent/WO2004048528A3/en
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

Links

Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L7/00—Heating or cooling apparatus; Heat insulating devices
    • B01L7/52—Heating or cooling apparatus; Heat insulating devices with provision for submitting samples to a predetermined sequence of different temperatures, e.g. for treating nucleic acid samples
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6851—Quantitative amplification
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/02—Burettes; Pipettes
    • B01L3/0289—Apparatus for withdrawing or distributing predetermined quantities of fluid
    • B01L3/0293—Apparatus for withdrawing or distributing predetermined quantities of fluid for liquids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/158—Expression markers
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
    • G01N2035/00465—Separating and mixing arrangements
    • G01N2035/00495—Centrifuges
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
    • G01N35/02—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor using a plurality of sample containers moved by a conveyor system past one or more treatment or analysis stations
    • G01N35/04—Details of the conveyor system
    • G01N2035/0401—Sample carriers, cuvettes or reaction vessels
    • G01N2035/0403—Sample carriers with closing or sealing means
    • G01N2035/0405—Sample carriers with closing or sealing means manipulating closing or opening means, e.g. stoppers, screw caps, lids or covers
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
    • G01N35/10—Devices for transferring samples or any liquids to, in, or from, the analysis apparatus, e.g. suction devices, injection devices
    • G01N2035/1027—General features of the devices
    • G01N2035/103—General features of the devices using disposable tips

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Molecular Biology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • General Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Immunology (AREA)
  • Analytical Chemistry (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Physics & Mathematics (AREA)
  • Genetics & Genomics (AREA)
  • Clinical Laboratory Science (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Other Investigation Or Analysis Of Materials By Electrical Means (AREA)

Abstract

The invention provides sampling methods and apparatuses that permit the quantitative analysis of nucleic acid amplification reactions. Quantitative information garnered during an amplification regimen permits the development of a detailed amplification profile that in turn permits the reliable determination of original template abundance. The methods disclosed are particularly useful for quantitative expression analysis of multiple genes or transcription units, both in the same amplification reaction and in multiple amplification reactions.
PCT/US2003/037420 2002-11-21 2003-11-21 Sampling method and apparatus for amplification reaction analysis Ceased WO2004048528A2 (en)

Priority Applications (4)

Application Number Priority Date Filing Date Title
CA002509769A CA2509769A1 (en) 2002-11-21 2003-11-21 Sampling method and apparatus for amplification reaction analysis
AU2003291144A AU2003291144A1 (en) 2002-11-21 2003-11-21 Sampling method and apparatus for amplification reaction analysis
JP2005510386A JP2006507849A (en) 2002-11-21 2003-11-21 Sampling method and apparatus for amplification reaction analysis
EP03783738A EP1563097A4 (en) 2002-11-21 2003-11-21 Sampling method and apparatus for amplification reaction analysis

Applications Claiming Priority (6)

Application Number Priority Date Filing Date Title
US42803802P 2002-11-21 2002-11-21
US60/428,038 2002-11-21
US43998203P 2003-01-14 2003-01-14
US44001003P 2003-01-14 2003-01-14
US60/439,982 2003-01-14
US60/440,010 2003-01-14

Publications (2)

Publication Number Publication Date
WO2004048528A2 WO2004048528A2 (en) 2004-06-10
WO2004048528A3 true WO2004048528A3 (en) 2004-11-11

Family

ID=32397974

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2003/037420 Ceased WO2004048528A2 (en) 2002-11-21 2003-11-21 Sampling method and apparatus for amplification reaction analysis

Country Status (6)

Country Link
EP (1) EP1563097A4 (en)
JP (1) JP2006507849A (en)
KR (1) KR20050075436A (en)
AU (1) AU2003291144A1 (en)
CA (1) CA2509769A1 (en)
WO (1) WO2004048528A2 (en)

Families Citing this family (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20030077611A1 (en) 2001-10-24 2003-04-24 Sention Methods and systems for dynamic gene expression profiling
US7445893B2 (en) 2002-04-12 2008-11-04 Primera Biosystems, Inc. Sampling method for amplification reaction analysis
US7081339B2 (en) 2002-04-12 2006-07-25 Primera Biosystems, Inc. Methods for variation detection
JP4981295B2 (en) * 2005-10-04 2012-07-18 キヤノン株式会社 DNA processing method and DNA processing apparatus
EP2021113A2 (en) * 2006-05-11 2009-02-11 Raindance Technologies, Inc. Microfluidic devices
DE102013200193A1 (en) 2013-01-09 2014-07-10 Hamilton Bonaduz Ag Sample processing system with dosing device and thermocycler
KR101816288B1 (en) * 2016-08-11 2018-01-09 한국기초과학지원연구원 Method of pre-treating sample with super absorbent polymer and device therefor
WO2022132821A1 (en) * 2020-12-15 2022-06-23 Quantum-Si Incorporated Systems and methods for chip regeneration
JP7090256B1 (en) 2022-01-14 2022-06-24 ヨダカ技研株式会社 Temperature control device, drive device, gene amplification system, gene amplification method, and gene amplification program
WO2025009019A1 (en) * 2023-07-03 2025-01-09 株式会社日立ハイテク Dna analysis system

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20020028452A1 (en) * 1999-03-30 2002-03-07 Wittwer Carl T. Method for quantification of an analyte
US6482615B2 (en) * 2001-03-02 2002-11-19 Integrated Genetic Devices Ltd. Method and apparatus for effecting rapid thermal cycling of samples in microtiter plate size
US6495326B2 (en) * 1999-04-20 2002-12-17 Japan Bioindustry Association Method for determining a concentration of target nucleic acid molecules, nucleic acid probes for the method, and method for analyzing data obtained by the method
US6531282B1 (en) * 2000-05-30 2003-03-11 Oligotrail, Llc Multiplex amplification and analysis of selected STR loci

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2278465C (en) * 1997-01-30 2010-04-27 University Technology Corporation Diagnosis and treatment of myocardial failure
CA2369363A1 (en) * 1999-04-29 2000-11-09 Marcy Engelstein Device for rapid dna sample processing with integrated liquid handling, thermocycling, and purification
WO2001055454A1 (en) * 2000-01-28 2001-08-02 Althea Technologies, Inc. Methods for analysis of gene expression
AU2001286756A1 (en) * 2000-08-25 2002-03-04 Genome Therapeutics Corporation Device for identifying the presence of a nucleotide sequence in a dna sample
US20030077611A1 (en) * 2001-10-24 2003-04-24 Sention Methods and systems for dynamic gene expression profiling

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20020028452A1 (en) * 1999-03-30 2002-03-07 Wittwer Carl T. Method for quantification of an analyte
US6495326B2 (en) * 1999-04-20 2002-12-17 Japan Bioindustry Association Method for determining a concentration of target nucleic acid molecules, nucleic acid probes for the method, and method for analyzing data obtained by the method
US6531282B1 (en) * 2000-05-30 2003-03-11 Oligotrail, Llc Multiplex amplification and analysis of selected STR loci
US6482615B2 (en) * 2001-03-02 2002-11-19 Integrated Genetic Devices Ltd. Method and apparatus for effecting rapid thermal cycling of samples in microtiter plate size

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
See also references of EP1563097A4 *

Also Published As

Publication number Publication date
KR20050075436A (en) 2005-07-20
JP2006507849A (en) 2006-03-09
WO2004048528A2 (en) 2004-06-10
AU2003291144A1 (en) 2004-06-18
EP1563097A4 (en) 2006-03-15
CA2509769A1 (en) 2004-06-10
EP1563097A2 (en) 2005-08-17

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