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WO2003106032A8 - Hybridization Chamber - Google Patents

Hybridization Chamber

Info

Publication number
WO2003106032A8
WO2003106032A8 PCT/EP2003/006253 EP0306253W WO03106032A8 WO 2003106032 A8 WO2003106032 A8 WO 2003106032A8 EP 0306253 W EP0306253 W EP 0306253W WO 03106032 A8 WO03106032 A8 WO 03106032A8
Authority
WO
WIPO (PCT)
Prior art keywords
microarray
hybridization chamber
cover
base
cover surface
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/EP2003/006253
Other languages
German (de)
French (fr)
Other versions
WO2003106032A1 (en
Inventor
Ralph Mueller
Torsten Gerboth
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Sygnis Pharma AG
Original Assignee
Axaron Bioscience AG
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from DE10254564A external-priority patent/DE10254564A1/en
Application filed by Axaron Bioscience AG filed Critical Axaron Bioscience AG
Priority to AU2003238501A priority Critical patent/AU2003238501A1/en
Publication of WO2003106032A1 publication Critical patent/WO2003106032A1/en
Publication of WO2003106032A8 publication Critical patent/WO2003106032A8/en
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

Links

Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/06—Auxiliary integrated devices, integrated components
    • B01L2300/0627—Sensor or part of a sensor is integrated
    • B01L2300/0636—Integrated biosensor, microarrays
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0809—Geometry, shape and general structure rectangular shaped
    • B01L2300/0822—Slides
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0861—Configuration of multiple channels and/or chambers in a single devices
    • B01L2300/0877—Flow chambers
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/04—Moving fluids with specific forces or mechanical means
    • B01L2400/0403—Moving fluids with specific forces or mechanical means specific forces
    • B01L2400/0406—Moving fluids with specific forces or mechanical means specific forces capillary forces
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502707—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by the manufacture of the container or its components
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502715—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by interfacing components, e.g. fluidic, electrical, optical or mechanical interfaces
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/50273—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by the means or forces applied to move the fluids
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/508—Containers for the purpose of retaining a material to be analysed, e.g. test tubes rigid containers not provided for above
    • B01L3/5088—Containers for the purpose of retaining a material to be analysed, e.g. test tubes rigid containers not provided for above confining liquids at a location by surface tension, e.g. virtual wells on plates, wires

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Dispersion Chemistry (AREA)
  • Analytical Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Hematology (AREA)
  • Clinical Laboratory Science (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Automatic Analysis And Handling Materials Therefor (AREA)
  • Sampling And Sample Adjustment (AREA)

Abstract

L'invention concerne une chambre d'hybridation comprenant une base (10) dans laquelle est ménagé un évidement (14) destiné à loger un microréseau (16). Cette chambre comprend en outre un couvercle (12) qui peut être disposé sur la base (10), ce qui permet de former un espace hermétiquement clos pour le microréseau. Le couvercle (12) comporte une surface de couvercle (18) qui est espacée du microréseau d'approximativement 20 mu m et s'étend parallèlement à celui-ci. Une solution échantillon (38) est attirée sous l'effet de forces capillaires dans l'espace situé entre le microréseau et la surface de couvercle et s'y répartit uniformément sans qu'aucune pompe soit nécessaire. La chambre d'hybridation selon l'invention permet de travailler avec des quantités d'échantillon moindres sans pertes de volumes.The invention relates to a hybridization chamber comprising a base (10) in which is formed a recess (14) intended to accommodate a microarray (16). This chamber further comprises a cover (12) which can be arranged on the base (10), which makes it possible to form a hermetically sealed space for the microarray. The cover (12) has a cover surface (18) which is spaced from the microarray by approximately 20 µm and extends parallel to it. A sample solution (38) is drawn under the effect of capillary forces into the space between the microarray and the cover surface and is distributed there evenly without the need for a pump. The hybridization chamber according to the invention makes it possible to work with smaller quantities of sample without loss of volumes.

PCT/EP2003/006253 2002-06-14 2003-06-13 Hybridization chamber Ceased WO2003106032A1 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU2003238501A AU2003238501A1 (en) 2002-06-14 2003-06-13 Hybridization chamber

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
DE10226750.2 2002-06-14
DE10226750 2002-06-14
DE10254564A DE10254564A1 (en) 2002-06-14 2002-11-21 hybridization chamber
DE10254564.2 2002-11-21

Publications (2)

Publication Number Publication Date
WO2003106032A1 WO2003106032A1 (en) 2003-12-24
WO2003106032A8 true WO2003106032A8 (en) 2004-02-26

Family

ID=29737606

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/EP2003/006253 Ceased WO2003106032A1 (en) 2002-06-14 2003-06-13 Hybridization chamber

Country Status (2)

Country Link
AU (1) AU2003238501A1 (en)
WO (1) WO2003106032A1 (en)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE102004046724B3 (en) * 2004-09-27 2006-02-02 Zell-Kontakt Gmbh Reaction container, comprises outlet surrounding reaction surface and provides supporting cover unit in order to form reaction chamber by arranging plane central area of cover unit in preset distance to surface
DE202007016855U1 (en) * 2007-11-30 2008-02-14 DKFZ Deutsches Krebsforschungszentrum Stiftung des öffentlichen Rechts Incubation tray for protein array incubations
DE202009008601U1 (en) 2009-06-19 2009-09-24 Deutsches Krebsforschungszentrum Stiftung des öffentlichen Rechts Incubation tray for high throughput procedures in protein array incubations

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5051237A (en) * 1988-06-23 1991-09-24 P B Diagnostic Systems, Inc. Liquid transport system
WO1992007655A1 (en) * 1990-10-30 1992-05-14 Hypoguard (Uk) Limited Collection and display device
US5849486A (en) * 1993-11-01 1998-12-15 Nanogen, Inc. Methods for hybridization analysis utilizing electrically controlled hybridization
US6287850B1 (en) * 1995-06-07 2001-09-11 Affymetrix, Inc. Bioarray chip reaction apparatus and its manufacture
DE19610146C1 (en) * 1996-03-15 1997-06-12 Wolf Prof Dr Bertling Container for biological or medical samples comprising body and lid
WO2001041931A2 (en) * 1999-12-09 2001-06-14 Motorola, Inc. Multilayered microfluidic devices for analyte reactions
DE10142788A1 (en) * 2001-08-31 2003-03-27 Advalytix Ag To form a thin liquid film on a carrier, for chemical/biological sample analysis, the flat carrier is shrouded by a spaced cover, for liquid to pass through a passage drilling and spread by capillary action

Also Published As

Publication number Publication date
AU2003238501A8 (en) 2003-12-31
WO2003106032A1 (en) 2003-12-24
AU2003238501A1 (en) 2003-12-31

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