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WO2003039459A3 - Viral vector production methods and compositions - Google Patents

Viral vector production methods and compositions Download PDF

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Publication number
WO2003039459A3
WO2003039459A3 PCT/US2002/035049 US0235049W WO03039459A3 WO 2003039459 A3 WO2003039459 A3 WO 2003039459A3 US 0235049 W US0235049 W US 0235049W WO 03039459 A3 WO03039459 A3 WO 03039459A3
Authority
WO
WIPO (PCT)
Prior art keywords
viral vector
compositions
production methods
vector production
provides methods
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/US2002/035049
Other languages
French (fr)
Other versions
WO2003039459A2 (en
Inventor
Lee-Cheng Liu
Perry Newton
Shoupeng Lai
Stephen Morris
Chad Atwell
Christon Hill
Megan Fitzpatrick
Sami Cardak
Alena Lizonova
Lu Qin
Miguel E Carrion
Brenk K Harris
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Genvec Inc
Original Assignee
Genvec Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Genvec Inc filed Critical Genvec Inc
Priority to AU2002348151A priority Critical patent/AU2002348151A1/en
Publication of WO2003039459A2 publication Critical patent/WO2003039459A2/en
Publication of WO2003039459A3 publication Critical patent/WO2003039459A3/en
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C12N15/86—Viral vectors
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/08—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from viruses
    • C07K16/081—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from viruses from DNA viruses
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2710/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA dsDNA viruses
    • C12N2710/00011—Details
    • C12N2710/10011—Adenoviridae
    • C12N2710/10311—Mastadenovirus, e.g. human or simian adenoviruses
    • C12N2710/10341—Use of virus, viral particle or viral elements as a vector
    • C12N2710/10343—Use of virus, viral particle or viral elements as a vector viral genome or elements thereof as genetic vector
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2710/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA dsDNA viruses
    • C12N2710/00011—Details
    • C12N2710/10011—Adenoviridae
    • C12N2710/10311—Mastadenovirus, e.g. human or simian adenoviruses
    • C12N2710/10351—Methods of production or purification of viral material

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Virology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Biomedical Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Immunology (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

The present invention provides methods of preparing viral vector particles and viral vector particle compositions.
PCT/US2002/035049 2001-11-05 2002-10-31 Viral vector production methods and compositions Ceased WO2003039459A2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU2002348151A AU2002348151A1 (en) 2001-11-05 2002-10-31 Viral vector production methods and compositions

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US33287301P 2001-11-05 2001-11-05
US60/332,873 2001-11-05
US33212001P 2001-11-13 2001-11-13
US60/332,120 2001-11-13

Publications (2)

Publication Number Publication Date
WO2003039459A2 WO2003039459A2 (en) 2003-05-15
WO2003039459A3 true WO2003039459A3 (en) 2003-11-20

Family

ID=26988068

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2002/035049 Ceased WO2003039459A2 (en) 2001-11-05 2002-10-31 Viral vector production methods and compositions

Country Status (2)

Country Link
AU (1) AU2002348151A1 (en)
WO (1) WO2003039459A2 (en)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US11911440B2 (en) 2009-05-02 2024-02-27 Genzyme Corporation Gene therapy for neurodegenerative disorders

Families Citing this family (26)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2007016250A1 (en) * 2005-07-28 2007-02-08 Genvec, Inc. Method of purifying protein
US20130052165A1 (en) * 2010-01-12 2013-02-28 Livnat Bangio Methods of Producing Adenovirus Vectors and Viral Preparations Generated Thereby
HUE036742T2 (en) 2011-11-24 2018-07-30 Genethon Scalable lentivirus vector production system compatible with industrial medical applications
WO2013154928A1 (en) * 2012-04-08 2013-10-17 Kapre Subhash V Systems and methods of virus propagation in cell culture for vaccine manufacture
MX367025B (en) 2013-02-22 2019-08-02 Genzyme Corp Microcarrier perfusion culturing methods and uses thereof.
HUE042094T2 (en) * 2013-02-22 2019-06-28 Genzyme Corp Microcarrier perfusion culturing methods and uses thereof
US20140323556A1 (en) 2013-03-15 2014-10-30 The Children's Hospital Of Philadelphia Scalable manufacturing process to produce recombinant lentiviral vectors in serum-free suspension cell culture system
WO2015010030A1 (en) * 2013-07-18 2015-01-22 Children's Hospital Medical Center Methods of improving titer in transfection-based production systems using eukaryotic cells
US11008547B2 (en) 2014-03-25 2021-05-18 Terumo Bct, Inc. Passive replacement of media
TW202204596A (en) 2014-06-06 2022-02-01 美商健臻公司 Perfusion culturing methods and uses thereof
TWI707949B (en) 2014-06-09 2020-10-21 美商健臻公司 Seed train processes and uses thereof
KR20250025516A (en) 2014-12-22 2025-02-21 젠자임 코포레이션 Methods of culturing a mammalian cell
EP3054007A1 (en) 2015-02-09 2016-08-10 Institut National De La Sante Et De La Recherche Medicale (Inserm) Recombinant adeno-associated virus particle purification comprising an immuno-affinity purification step
WO2017004592A1 (en) 2015-07-02 2017-01-05 Terumo Bct, Inc. Cell growth with mechanical stimuli
CN109415696A (en) 2016-05-25 2019-03-01 泰尔茂比司特公司 Cell amplification
US11685883B2 (en) 2016-06-07 2023-06-27 Terumo Bct, Inc. Methods and systems for coating a cell growth surface
US11104874B2 (en) 2016-06-07 2021-08-31 Terumo Bct, Inc. Coating a bioreactor
MX2019004784A (en) * 2016-11-04 2019-08-12 Baxalta Inc Adeno-associated virus purification methods.
EP3601521A2 (en) 2017-03-31 2020-02-05 Terumo BCT, Inc. Cell expansion
AU2018253088B2 (en) 2017-04-10 2024-09-19 Epicentrx, Inc. Method for producing recombinant virus
US11603527B2 (en) * 2017-12-27 2023-03-14 Global Life Sciences Solutions Usa Llc Method and kit for viral vector isolation
CN110317832B (en) 2018-03-28 2022-07-05 西比曼生物科技（香港）有限公司 Method for preparing purified preparation of recombinant lentiviral vector on GMP scale
CN110317791A (en) 2018-03-29 2019-10-11 西比曼生物科技（香港）有限公司 The method of GMP grades of serum free suspension cell large-scale production slow virus
CN116590346B (en) * 2020-09-15 2024-05-14 上海药明巨诺生物医药研发有限公司 Lentiviral vector purification method
EP4232081A4 (en) 2020-10-23 2025-05-07 Resilience Government Services, Inc. METHOD FOR INFECTING CELLS WITH A VIRUS
IL307715A (en) * 2021-04-14 2023-12-01 Genzyme Corp Methods of perfusion culturing a mammalian cell

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6143548A (en) * 1995-08-30 2000-11-07 Genzyme Corporation Chromatographic purification of adeno-associated virus (AAV)
US6168941B1 (en) * 2000-04-07 2001-01-02 Genvec, Inc. Method of producing adenoviral vector stocks
US6194191B1 (en) * 1996-11-20 2001-02-27 Introgen Therapeutics, Inc. Method for the production and purification of adenoviral vectors
US6303362B1 (en) * 1998-11-19 2001-10-16 The Board Of Trustees Of The Leland Stanford Junior University Adenoviral vector and methods for making and using the same
US20010036657A1 (en) * 1996-12-13 2001-11-01 Tang John Chu-Tay Methods for purifying viruses

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6143548A (en) * 1995-08-30 2000-11-07 Genzyme Corporation Chromatographic purification of adeno-associated virus (AAV)
US6194191B1 (en) * 1996-11-20 2001-02-27 Introgen Therapeutics, Inc. Method for the production and purification of adenoviral vectors
US20010036657A1 (en) * 1996-12-13 2001-11-01 Tang John Chu-Tay Methods for purifying viruses
US6303362B1 (en) * 1998-11-19 2001-10-16 The Board Of Trustees Of The Leland Stanford Junior University Adenoviral vector and methods for making and using the same
US6168941B1 (en) * 2000-04-07 2001-01-02 Genvec, Inc. Method of producing adenoviral vector stocks

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US11911440B2 (en) 2009-05-02 2024-02-27 Genzyme Corporation Gene therapy for neurodegenerative disorders
US11975043B2 (en) 2009-05-02 2024-05-07 Genzyme Corporation Gene therapy for neurodegenerative disorders
US12350311B2 (en) 2009-05-02 2025-07-08 Genzyme Corporation Gene therapy for neurodegenerative disorders

Also Published As

Publication number Publication date
WO2003039459A2 (en) 2003-05-15
AU2002348151A1 (en) 2003-05-19

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