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WO2003035829A3 - Nanoparticles having oligonucleotides attached thereto and uses therefor - Google Patents

Nanoparticles having oligonucleotides attached thereto and uses therefor Download PDF

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Publication number
WO2003035829A3
WO2003035829A3 PCT/US2002/032088 US0232088W WO03035829A3 WO 2003035829 A3 WO2003035829 A3 WO 2003035829A3 US 0232088 W US0232088 W US 0232088W WO 03035829 A3 WO03035829 A3 WO 03035829A3
Authority
WO
WIPO (PCT)
Prior art keywords
nanoparticles
methods
nucleic acid
provides
oligonucleotides
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/US2002/032088
Other languages
French (fr)
Other versions
WO2003035829A2 (en
Inventor
So-Jung Park
Thomas A Taton
Chad A Mirkin
Robert L Letsinger
Robert C Mucic
James J Storhoff
Robert Elghanian
Viswanadham Garimella
Zhi Li
Gang Lu
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Nanosphere LLC
Original Assignee
Nanosphere LLC
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from US10/008,978 external-priority patent/US6984491B2/en
Application filed by Nanosphere LLC filed Critical Nanosphere LLC
Priority to AU2002363062A priority Critical patent/AU2002363062B2/en
Priority to CA2463323A priority patent/CA2463323C/en
Priority to JP2003538330A priority patent/JP4347049B2/en
Priority to EP02799155A priority patent/EP1478774A4/en
Publication of WO2003035829A2 publication Critical patent/WO2003035829A2/en
Anticipated expiration legal-status Critical
Publication of WO2003035829A3 publication Critical patent/WO2003035829A3/en
Ceased legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Immunology (AREA)
  • Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

The invention provides methods of detecting a nucleic acid. The methods comprise contacting the nucleic acid with one or more types of particles having oligonucleotides attached thereto. In one embodiment of the method, the oligonucleotides are attached to nanoparticles and have sequences complementary to portions of the sequence of the nucleic acid. A detectable change (preferably a color change) is brought about as a result of the hybridization of the oligonucleotides on the nanoparticles to the nucleic acid. The invention also provides compositions and kits comprising particles. The invention further provides methods of synthesizing unique nanoparticle-oligonucleotide conjugates, the conjugates produced by the methods, and methods of using the conjugates. In addition, the invention provides nanomaterials and nanostructures comprising nanoparticles and methods of nanofabrication utilizing nanoparticles. Finally, the invention provides a method of separating a selected nucleic acid from other nucleic acids.
PCT/US2002/032088 2001-10-09 2002-10-08 Nanoparticles having oligonucleotides attached thereto and uses therefor Ceased WO2003035829A2 (en)

Priority Applications (4)

Application Number Priority Date Filing Date Title
AU2002363062A AU2002363062B2 (en) 2001-10-09 2002-10-08 Nanoparticles having oligonucleotides attached thereto and uses therefor
CA2463323A CA2463323C (en) 2001-10-09 2002-10-08 Nanoparticles having oligonucleotides attached thereto and uses therefor
JP2003538330A JP4347049B2 (en) 2001-10-09 2002-10-08 Nanoparticles with oligonucleotides attached and methods of use
EP02799155A EP1478774A4 (en) 2001-10-09 2002-10-08 Nanoparticles having oligonucleotides attached thereto and uses therefor

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US32786401P 2001-10-09 2001-10-09
US60/327,864 2001-10-09
US10/008,978 2001-12-07
US10/008,978 US6984491B2 (en) 1996-07-29 2001-12-07 Nanoparticles having oligonucleotides attached thereto and uses therefor

Publications (2)

Publication Number Publication Date
WO2003035829A2 WO2003035829A2 (en) 2003-05-01
WO2003035829A3 true WO2003035829A3 (en) 2004-08-26

Family

ID=32684383

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2002/032088 Ceased WO2003035829A2 (en) 2001-10-09 2002-10-08 Nanoparticles having oligonucleotides attached thereto and uses therefor

Country Status (5)

Country Link
EP (1) EP1478774A4 (en)
JP (1) JP4347049B2 (en)
AU (1) AU2002363062B2 (en)
CA (1) CA2463323C (en)
WO (1) WO2003035829A2 (en)

Cited By (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6962786B2 (en) 1996-07-29 2005-11-08 Nanosphere, Inc. Nanoparticles having oligonucleotides attached thereto and uses therefor
US6974669B2 (en) 2000-03-28 2005-12-13 Nanosphere, Inc. Bio-barcodes based on oligonucleotide-modified nanoparticles
US6986989B2 (en) 1996-07-29 2006-01-17 Nanosphere, Inc. Nanoparticles having oligonucleotides attached thereto and uses therefor
US7098320B1 (en) 1996-07-29 2006-08-29 Nanosphere, Inc. Nanoparticles having oligonucleotides attached thereto and uses therefor
US7115688B1 (en) 1998-11-30 2006-10-03 Nanosphere, Inc. Nanoparticles with polymer shells
US7135055B2 (en) 2001-05-25 2006-11-14 Nanosphere, Inc. Non-alloying core shell nanoparticles
US7147687B2 (en) 2001-05-25 2006-12-12 Nanosphere, Inc. Non-alloying core shell nanoparticles
US7169556B2 (en) 1996-07-29 2007-01-30 Nanosphere, Inc. Nanoparticles having oligonucleotides attached thereto and uses therefor
US7208587B2 (en) 1996-07-29 2007-04-24 Nanosphere, Inc. Nanoparticles having oligonucleotides attached thereto and uses therefor
US7250499B2 (en) 1996-07-29 2007-07-31 Nanosphere Inc. Nanoparticles having oligonucleotides attached thereto and uses therefor
US7253277B2 (en) 2002-07-02 2007-08-07 Nanosphere, Inc. Nanoparticle polyanion conjugates and methods of use thereof in detecting analytes
US7985539B2 (en) 2002-05-07 2011-07-26 Northwestern University Nanoparticle probes with raman spectroscopic fingerprints for analyte detection
US11845986B2 (en) 2016-05-25 2023-12-19 Becton, Dickinson And Company Normalization of nucleic acid libraries

Families Citing this family (61)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7396677B2 (en) * 2003-11-07 2008-07-08 Nanosphere, Inc. Method of preparing nucleic acids for detection
JP4619012B2 (en) * 2004-02-19 2011-01-26 トヨタ自動車株式会社 Construction method of 3D DNA network
ES2242528B1 (en) 2004-03-25 2006-12-01 Consejo Sup. Investig. Cientificas MAGNETIC NANOPARTICLES OF NOBLE METALS.
JP2007534944A (en) * 2004-04-23 2007-11-29 オクソニカ・インコーポレーテッド Surface-enhanced spectroscopy activity-composite nanoparticles
EP2330208B1 (en) * 2004-05-24 2017-10-18 Midatech Ltd. Nanoparticles comprising RNA ligands
JP4822753B2 (en) * 2005-07-11 2011-11-24 一般社団法人オンチップ・セロミクス・コンソーシアム Cell component sorting chip, cell component analysis system, and cell component analysis method using them
KR100816419B1 (en) * 2005-10-14 2008-03-27 래플진(주) Method for Isothermal Amplification of Nucleic Acids and Method for Detecting Nucleic Acids Using Simultaneous Isothermal Amplification of Nucleic Acids and Signal Probe
EP1801593A1 (en) * 2005-12-22 2007-06-27 Koninklijke Philips Electronics N.V. A method of imaging biological specimens using inorganic nanoparticles as label agents
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JP5391744B2 (en) * 2009-03-09 2014-01-15 住友ベークライト株式会社 Array substrate
US8835358B2 (en) 2009-12-15 2014-09-16 Cellular Research, Inc. Digital counting of individual molecules by stochastic attachment of diverse labels
JP5200069B2 (en) * 2010-07-27 2013-05-15 トヨタ自動車株式会社 3D DNA network
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US9335292B2 (en) 2011-10-13 2016-05-10 Auburn University Electrochemical proximity assay
US10941396B2 (en) 2012-02-27 2021-03-09 Becton, Dickinson And Company Compositions and kits for molecular counting
US9567645B2 (en) 2013-08-28 2017-02-14 Cellular Research, Inc. Massively parallel single cell analysis
CN106062210A (en) 2013-12-23 2016-10-26 昆士兰大学 Nucleic acid detection method and kit
JPWO2015159535A1 (en) * 2014-04-16 2017-04-13 富士フイルム株式会社 Detection solution and its preparation method, biosensing method
WO2016138496A1 (en) 2015-02-27 2016-09-01 Cellular Research, Inc. Spatially addressable molecular barcoding
EP3277843A2 (en) 2015-03-30 2018-02-07 Cellular Research, Inc. Methods and compositions for combinatorial barcoding
WO2016172373A1 (en) 2015-04-23 2016-10-27 Cellular Research, Inc. Methods and compositions for whole transcriptome amplification
CN107636162B (en) 2015-06-01 2021-12-10 加利福尼亚技术学院 Compositions and methods for screening T cells with antigens directed to specific populations
WO2017044574A1 (en) 2015-09-11 2017-03-16 Cellular Research, Inc. Methods and compositions for nucleic acid library normalization
US11078475B2 (en) * 2016-05-03 2021-08-03 Sinai Health System Methods of capturing cell-free methylated DNA and uses of same
CA3023256A1 (en) * 2016-05-18 2017-11-23 Integrated Nano-Technologies, Inc. Method for detection of a pcr product
US10640763B2 (en) 2016-05-31 2020-05-05 Cellular Research, Inc. Molecular indexing of internal sequences
US10202641B2 (en) 2016-05-31 2019-02-12 Cellular Research, Inc. Error correction in amplification of samples
KR102522023B1 (en) 2016-09-26 2023-04-17 셀룰러 리서치, 인크. Measurement of protein expression using reagents with barcoded oligonucleotide sequences
WO2018119128A1 (en) * 2016-12-22 2018-06-28 Burris Robert Barton Methods for non-enzymatic amplification of a signal and uses thereof to detect and quantify a target analyte
EP3577232A1 (en) 2017-02-01 2019-12-11 Cellular Research, Inc. Selective amplification using blocking oligonucleotides
US12258613B2 (en) 2017-03-08 2025-03-25 California Institute Of Technology Pairing antigen specificity of a T cell with T cell receptor sequences
WO2018165566A1 (en) 2017-03-09 2018-09-13 Auburn University Differential circuit for background correction in electrochemical measurements
CA3059559A1 (en) 2017-06-05 2018-12-13 Becton, Dickinson And Company Sample indexing for single cells
US11505799B2 (en) 2017-07-07 2022-11-22 Innamed, Inc. Aptamers for measuring lipoprotein levels
KR20190011117A (en) * 2017-07-24 2019-02-01 사회복지법인 삼성생명공익재단 Method for detection of genetic mutation by using aggregation of gold nanoparticles
EP4234717A3 (en) 2018-05-03 2023-11-01 Becton, Dickinson and Company High throughput multiomics sample analysis
CN112243461B (en) 2018-05-03 2024-07-12 贝克顿迪金森公司 Molecular barcoding at opposite transcript ends
US11560565B2 (en) 2018-06-13 2023-01-24 Auburn University Electrochemical detection nanostructure, systems, and uses thereof
US20210275588A1 (en) * 2018-07-10 2021-09-09 The Regents Of The University Of California Biomolecule Coated Particles and Films and Uses Thereof
BR112020024296A2 (en) 2018-08-27 2021-03-09 Regeneron Pharmaceuticals, Inc. METHODS FOR THE PRODUCTION OF A CONCENTRATED PROTEIN PURIFICATION INTERMEDIATE, FOR MONITORING AND CONTROL OF CRITICAL QUALITY ATTRIBUTES IN A PROTEIN PURIFICATION INTERMEDIATE AND TO MONITOR AND CONTROL THE EXCIPIENT LEVELS IN THE CULTURE AND CULTURE FLUID FLUID. PROTEIN, AND, PROTEIN PURIFICATION INTERMEDIATE
EP3861134B1 (en) 2018-10-01 2024-09-04 Becton, Dickinson and Company Determining 5' transcript sequences
US11932849B2 (en) 2018-11-08 2024-03-19 Becton, Dickinson And Company Whole transcriptome analysis of single cells using random priming
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WO2020154247A1 (en) 2019-01-23 2020-07-30 Cellular Research, Inc. Oligonucleotides associated with antibodies
US12071617B2 (en) 2019-02-14 2024-08-27 Becton, Dickinson And Company Hybrid targeted and whole transcriptome amplification
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MX2023000345A (en) * 2020-07-08 2023-02-13 Illumina Inc Beads as transposome carriers.
US11932901B2 (en) 2020-07-13 2024-03-19 Becton, Dickinson And Company Target enrichment using nucleic acid probes for scRNAseq
WO2022026909A1 (en) 2020-07-31 2022-02-03 Becton, Dickinson And Company Single cell assay for transposase-accessible chromatin
US11739443B2 (en) 2020-11-20 2023-08-29 Becton, Dickinson And Company Profiling of highly expressed and lowly expressed proteins
CN116685850A (en) 2020-12-15 2023-09-01 贝克顿迪金森公司 Single cell secretome analysis
WO2022182125A1 (en) * 2021-02-23 2022-09-01 모던밸류 주식회사 Heat controller-integrated, turn-off-type droplet loading cartridge for measuring optical signal
CN113088517B (en) * 2021-03-15 2023-10-03 华南师范大学 Construction method of nano probe
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CN115369153B (en) * 2022-10-25 2023-03-10 中国医学科学院北京协和医院 Smad4 gene detection biosensor and preparation method thereof

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6025202A (en) * 1995-02-09 2000-02-15 The Penn State Research Foundation Self-assembled metal colloid monolayers and detection methods therewith
US6214560B1 (en) * 1996-04-25 2001-04-10 Genicon Sciences Corporation Analyte assay using particulate labels

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1301625B1 (en) * 2000-03-28 2010-11-03 Nanosphere, Inc. Nanoparticles having oligonucleotides attached thereto and uses therefor

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6025202A (en) * 1995-02-09 2000-02-15 The Penn State Research Foundation Self-assembled metal colloid monolayers and detection methods therewith
US6214560B1 (en) * 1996-04-25 2001-04-10 Genicon Sciences Corporation Analyte assay using particulate labels

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
See also references of EP1478774A4 *

Cited By (17)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7169556B2 (en) 1996-07-29 2007-01-30 Nanosphere, Inc. Nanoparticles having oligonucleotides attached thereto and uses therefor
US7259252B2 (en) 1996-07-29 2007-08-21 Nanosphere, Inc. Nanoparticles having oligonucleotides attached thereto and uses therefor
US8323888B2 (en) 1996-07-29 2012-12-04 Nanosphere, Inc. Nanoparticles having oligonucleotides attached thereto and uses therefor
US6986989B2 (en) 1996-07-29 2006-01-17 Nanosphere, Inc. Nanoparticles having oligonucleotides attached thereto and uses therefor
US7098320B1 (en) 1996-07-29 2006-08-29 Nanosphere, Inc. Nanoparticles having oligonucleotides attached thereto and uses therefor
US7250499B2 (en) 1996-07-29 2007-07-31 Nanosphere Inc. Nanoparticles having oligonucleotides attached thereto and uses therefor
US6962786B2 (en) 1996-07-29 2005-11-08 Nanosphere, Inc. Nanoparticles having oligonucleotides attached thereto and uses therefor
US7208587B2 (en) 1996-07-29 2007-04-24 Nanosphere, Inc. Nanoparticles having oligonucleotides attached thereto and uses therefor
US6969761B2 (en) 1996-07-29 2005-11-29 Nanosphere, Inc. Nanoparticles having oligonucleotides attached thereto and uses therefor
US7115688B1 (en) 1998-11-30 2006-10-03 Nanosphere, Inc. Nanoparticles with polymer shells
US6974669B2 (en) 2000-03-28 2005-12-13 Nanosphere, Inc. Bio-barcodes based on oligonucleotide-modified nanoparticles
US7147687B2 (en) 2001-05-25 2006-12-12 Nanosphere, Inc. Non-alloying core shell nanoparticles
US7135055B2 (en) 2001-05-25 2006-11-14 Nanosphere, Inc. Non-alloying core shell nanoparticles
US7238472B2 (en) 2001-05-25 2007-07-03 Nanosphere, Inc. Non-alloying core shell nanoparticles
US7985539B2 (en) 2002-05-07 2011-07-26 Northwestern University Nanoparticle probes with raman spectroscopic fingerprints for analyte detection
US7253277B2 (en) 2002-07-02 2007-08-07 Nanosphere, Inc. Nanoparticle polyanion conjugates and methods of use thereof in detecting analytes
US11845986B2 (en) 2016-05-25 2023-12-19 Becton, Dickinson And Company Normalization of nucleic acid libraries

Also Published As

Publication number Publication date
CA2463323C (en) 2010-12-21
CA2463323A1 (en) 2003-05-01
EP1478774A4 (en) 2006-11-29
AU2002363062B2 (en) 2007-03-22
EP1478774A2 (en) 2004-11-24
JP2005525084A (en) 2005-08-25
JP4347049B2 (en) 2009-10-21
WO2003035829A2 (en) 2003-05-01

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