[go: up one dir, main page]

WO2003033695A1 - Procede de purification de proteine fusionnee recombinee et procede de production de proteine utilisant ledit procede de purification - Google Patents

Procede de purification de proteine fusionnee recombinee et procede de production de proteine utilisant ledit procede de purification Download PDF

Info

Publication number
WO2003033695A1
WO2003033695A1 PCT/JP2002/010578 JP0210578W WO03033695A1 WO 2003033695 A1 WO2003033695 A1 WO 2003033695A1 JP 0210578 W JP0210578 W JP 0210578W WO 03033695 A1 WO03033695 A1 WO 03033695A1
Authority
WO
WIPO (PCT)
Prior art keywords
protein
recombinant fused
dockerin
fused protein
target protein
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/JP2002/010578
Other languages
English (en)
French (fr)
Inventor
Tsuyoshi Nomura
Hidekazu Nagaya
Akio Shimamura
Shinichi Naya
Toshimichi Kanaya
Kazuo Sakka
Kunio Ohmiya
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Katakura Industries Co Ltd
Original Assignee
Katakura Industries Co Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Katakura Industries Co Ltd filed Critical Katakura Industries Co Ltd
Priority to JP2003536422A priority Critical patent/JPWO2003033695A1/ja
Priority to EP02801543A priority patent/EP1441030A4/en
Priority to US10/490,737 priority patent/US20050106700A1/en
Publication of WO2003033695A1 publication Critical patent/WO2003033695A1/ja
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00Preparation of peptides or proteins
    • C12P21/02Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K1/00General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14Extraction; Separation; Purification
    • C07K1/16Extraction; Separation; Purification by chromatography
    • C07K1/22Affinity chromatography or related techniques based upon selective absorption processes
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K14/00Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/33Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Clostridium (G)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/11DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/62DNA sequences coding for fusion proteins
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6803General methods of protein analysis not limited to specific proteins or families of proteins
    • G01N33/6845Methods of identifying protein-protein interactions in protein mixtures
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K2319/00Fusion polypeptide
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K2319/00Fusion polypeptide
    • C07K2319/20Fusion polypeptide containing a tag with affinity for a non-protein ligand
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K2319/00Fusion polypeptide
    • C07K2319/70Fusion polypeptide containing domain for protein-protein interaction
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/195Assays involving biological materials from specific organisms or of a specific nature from bacteria
    • G01N2333/33Assays involving biological materials from specific organisms or of a specific nature from bacteria from Clostridium (G)

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Biomedical Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Medicinal Chemistry (AREA)
  • Microbiology (AREA)
  • Zoology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Urology & Nephrology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Immunology (AREA)
  • Wood Science & Technology (AREA)
  • Hematology (AREA)
  • Analytical Chemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Food Science & Technology (AREA)
  • Cell Biology (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Plant Pathology (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
PCT/JP2002/010578 2001-10-11 2002-10-11 Procede de purification de proteine fusionnee recombinee et procede de production de proteine utilisant ledit procede de purification Ceased WO2003033695A1 (fr)

Priority Applications (3)

Application Number Priority Date Filing Date Title
JP2003536422A JPWO2003033695A1 (ja) 2001-10-11 2002-10-11 組換え融合タンパク質の精製方法およびこれを利用するタンパク質の製造方法
EP02801543A EP1441030A4 (en) 2001-10-11 2002-10-11 PROCESS FOR CLEANING A RECOMBINANT FUSION PROTEIN AND METHOD FOR PRODUCING PROTEIN THEREWITH
US10/490,737 US20050106700A1 (en) 2001-10-11 2002-10-11 Method of purifying recombinant fused protein and method of producing protein using the same

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
JP2001314079 2001-10-11
JP2001-314079 2001-10-11

Publications (1)

Publication Number Publication Date
WO2003033695A1 true WO2003033695A1 (fr) 2003-04-24

Family

ID=19132446

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/JP2002/010578 Ceased WO2003033695A1 (fr) 2001-10-11 2002-10-11 Procede de purification de proteine fusionnee recombinee et procede de production de proteine utilisant ledit procede de purification

Country Status (4)

Country Link
US (1) US20050106700A1 (ja)
EP (1) EP1441030A4 (ja)
JP (1) JPWO2003033695A1 (ja)
WO (1) WO2003033695A1 (ja)

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2009028531A1 (ja) * 2007-08-27 2009-03-05 Katakura Industries Co., Ltd. ドックリンポリペプチドおよびそれを利用した組換え融合タンパク質の精製方法
JP2010518020A (ja) * 2007-02-02 2010-05-27 ベイラー リサーチ インスティテュート ヒト化ターゲティングモノクローナル抗体と複合体を形成する複数の可変抗原
JP2011528233A (ja) * 2008-07-16 2011-11-17 ベイラー リサーチ インスティテュート 最大限のgag及びnefを樹状細胞に対して標的化することに基づくhivワクチン
CN104844685A (zh) * 2015-06-12 2015-08-19 中国科学院植物研究所 一种变性抗原亲和纯化抗体方法

Families Citing this family (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU2008214032B2 (en) 2007-02-02 2012-06-28 Baylor Research Institute Vaccines based on targeting antigen to DCIR expressed on antigen-presenting cells
TWI422594B (zh) 2007-02-02 2014-01-11 Baylor Res Inst 經由樹狀細胞去唾液酸糖蛋白受體(dc-asgpr)接合抗原呈現細胞之藥劑
BRPI0807617A2 (pt) * 2007-02-23 2014-07-22 Baylor Res Inst Aplicações terapêuticas de ativação de antígeno humano presente em células através de dectin-1.
US20110151538A1 (en) * 2008-08-07 2011-06-23 Ramot At Tel-Aviv University Ltd. Affinity purification by cohesin-dockerin interaction
CN103045627B (zh) * 2012-11-09 2015-04-15 逢甲大学 于细胞内制造蛋白复合物的方法
CA2949081C (en) 2014-05-16 2023-03-07 Baylor Research Institute Methods and compositions for treating autoimmune and inflammatory conditions
WO2016118787A1 (en) * 2015-01-21 2016-07-28 University Of Florida Research Foundation, Inc. Engineered receptor/ligand system for delivery of therapeutic agents
CA3114549A1 (en) 2017-02-21 2018-08-30 University Of Florida Research Foundation, Incorporated Modified aav capsid proteins and uses thereof
CN111518851B (zh) * 2019-11-06 2023-05-23 上海健康医学院 一种固定化酶连续制备[14/15n]-l-瓜氨酸的方法

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1994016083A1 (en) * 1993-01-15 1994-07-21 Genetics Institute, Inc. Cloning of enterokinase and method of use
JP2000157282A (ja) * 1998-11-30 2000-06-13 Toyota Central Res & Dev Lab Inc 酵素配列複合体及び固定化酵素配列複合体とそれらの製造方法、担体分子及び酵素

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU726108B2 (en) * 1995-10-17 2000-11-02 Genencor International, Inc. Enzymatic array and process of making same

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1994016083A1 (en) * 1993-01-15 1994-07-21 Genetics Institute, Inc. Cloning of enterokinase and method of use
JP2000157282A (ja) * 1998-11-30 2000-06-13 Toyota Central Res & Dev Lab Inc 酵素配列複合体及び固定化酵素配列複合体とそれらの製造方法、担体分子及び酵素

Non-Patent Citations (6)

* Cited by examiner, † Cited by third party
Title
KAKIUCHI M. ET AL.: "Cloning and DNA sequencing of the genes encoding Clostridium josui scaffoloding protein CipA and cellulase CelD and identification of their gene products as major components of the cellulosome", JOURNAL OF BACTERIOLOGY, vol. 180, no. 16, August 1998 (1998-08-01), pages 4303 - 4308, XP002966853 *
PAGES S. ET AL.: "Interaction between the endoglucanase CelA and the scaffolding protein CipC of the Clostridium cellulolyticum cellulosome", JOURNAL OF BACTERIOLOGY, vol. 178, no. 8, April 1996 (1996-04-01), pages 2279 - 2286, XP002966851 *
PARK J.S. ET AL.: "Cohesin-dockerin interactions of cellulosomal subunits of Clostridium cellulovorans", JOURNAL OF BACTERIOLOGY, vol. 183, no. 18, September 2001 (2001-09-01), pages 5431 - 5435, XP002966852 *
See also references of EP1441030A4 *
STOFKO-HAHN R.E. ET AL.: "A single step purification for recombinant proteins characterization of a microtuble associated protein (MAP2) fragment which associates with the type II cAMP-dependent protein kinase", FEBS LETTERS, vol. 302, no. 3, May 1992 (1992-05-01), pages 274 - 278, XP000267798 *
TAVARES G.A. ET AL.: "The crystal structure of a type I cohesin domain at 1.7 a resolution", JOURNAL OF MOLECULAR BIOLOGY, vol. 273, no. 3, October 1997 (1997-10-01), pages 701 - 713, XP002966850 *

Cited By (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2010518020A (ja) * 2007-02-02 2010-05-27 ベイラー リサーチ インスティテュート ヒト化ターゲティングモノクローナル抗体と複合体を形成する複数の可変抗原
WO2009028531A1 (ja) * 2007-08-27 2009-03-05 Katakura Industries Co., Ltd. ドックリンポリペプチドおよびそれを利用した組換え融合タンパク質の精製方法
US8536302B2 (en) 2007-08-27 2013-09-17 Sysmex Corporation Dockerin polypeptide and method of purifying recombinant fused protein using the same
JP5307716B2 (ja) * 2007-08-27 2013-10-02 シスメックス株式会社 ドックリンポリペプチドおよびそれを利用した組換え融合タンパク質の精製方法
JP2011528233A (ja) * 2008-07-16 2011-11-17 ベイラー リサーチ インスティテュート 最大限のgag及びnefを樹状細胞に対して標的化することに基づくhivワクチン
JP2015071598A (ja) * 2008-07-16 2015-04-16 ベイラー リサーチ インスティテュートBaylor Research Institute 最大限のgag及びnefを樹状細胞に対して標的化することに基づくhivワクチン
US9109011B2 (en) 2008-07-16 2015-08-18 Baylor Research Institute Dendritic cell-specific antibody conjugate comprising anti-CD40 monoclonal antibodies conjugated to HIV-1 Gag/Nef
CN104844685A (zh) * 2015-06-12 2015-08-19 中国科学院植物研究所 一种变性抗原亲和纯化抗体方法
CN104844685B (zh) * 2015-06-12 2018-10-16 中国科学院植物研究所 一种变性抗原亲和纯化抗体方法

Also Published As

Publication number Publication date
US20050106700A1 (en) 2005-05-19
EP1441030A4 (en) 2007-08-15
EP1441030A1 (en) 2004-07-28
JPWO2003033695A1 (ja) 2005-02-03

Similar Documents

Publication Publication Date Title
WO2003033695A1 (fr) Procede de purification de proteine fusionnee recombinee et procede de production de proteine utilisant ledit procede de purification
ATE301187T1 (de) Plastidtransformation in arabidopsis thaliana
WO2003000840A3 (en) Nitrilases
PL352332A1 (en) Expression and export of interferon-alpha proteins as fc fusion proteins
WO2001029242A3 (en) Post-translational modification of recombinant proteins produced in plants
WO2000011033A3 (en) Expression and export of angiostatin and endostatin as immunofusins
GB0129105D0 (en) Expression control using variable intergenic sequences
WO2000023593A3 (en) Molecular pathogenicide mediated plant disease resistance
WO2003097810A3 (en) Nitrilases, nucleic acids encoding them and methods for making and using them
ATE488584T1 (de) Verfahren und materialien zur erzeugung von sh3 domänen mit angepassten bindungseigenschaften
ATE278785T1 (de) Neues verfahren zur gewinnung und reinigung von fusionsproteinen
WO2001064889A3 (en) Tnf-alpha variants for the treatment of tnf-alpha related disorders
ATE417056T1 (de) Verfahren zur proteinreinigung
WO2003076561A3 (en) Recombinase fusion protein with enhanced cellular uptake
DE69835813D1 (de) Verfahren zur kultivierung von zellen und zur vermehrung von viren
WO2002016611A3 (en) Interleukin-22 polypeptides, nucleic acids encoding the same and methods for the treatment of pancreatic disorders
EP1358318B8 (en) Hybridoma cell line g250 and its use for producing monoclonal antibodies
AU2003247537A1 (en) Chd5 encoding nucleic acids, polypeptides, antibodies and methods of use thereof
WO2006018650A3 (en) Method of in vitro polypeptide selection using the arc dna binding domain fused to the polypeptide to be selected
WO2001064912A3 (en) Triazine degrading enzymes
WO2004087912A8 (en) Inhibitor proteins of a protease and use thereof
WO2001083754A3 (en) Cellular regulators of infectious agents and methods of use
WO2002088338A3 (fr) Procede de replication du virus de l'hepatite c
WO2001066752A3 (en) Reproduction-specific genes
WO2004000007A3 (en) Methods of modulating cytokinin related processes in a plant using b3 domain proteins

Legal Events

Date Code Title Description
AK Designated states

Kind code of ref document: A1

Designated state(s): AE AG AL AM AT AU AZ BA BB BG BY BZ CA CH CN CO CR CU CZ DE DM DZ EC EE ES FI GB GD GE GH HR HU ID IL IN IS JP KE KG KP KR LC LK LR LS LT LU LV MA MD MG MN MW MX MZ NO NZ OM PH PL PT RU SD SE SG SI SK SL TJ TM TN TR TZ UA UG US UZ VC VN YU ZA ZM

AL Designated countries for regional patents

Kind code of ref document: A1

Designated state(s): GH GM KE LS MW MZ SD SL SZ UG ZM ZW AM AZ BY KG KZ RU TJ TM AT BE BG CH CY CZ DK EE ES FI FR GB GR IE IT LU MC PT SE SK TR BF BJ CF CG CI GA GN GQ GW ML MR NE SN TD TG

DFPE Request for preliminary examination filed prior to expiration of 19th month from priority date (pct application filed before 20040101)
121 Ep: the epo has been informed by wipo that ep was designated in this application
WWE Wipo information: entry into national phase

Ref document number: 2003536422

Country of ref document: JP

WWE Wipo information: entry into national phase

Ref document number: 2002801543

Country of ref document: EP

WWE Wipo information: entry into national phase

Ref document number: 10490737

Country of ref document: US

WWP Wipo information: published in national office

Ref document number: 2002801543

Country of ref document: EP